0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10 Epilimnion No. of ... - Nature

(A)

No. of lakes where the read proportion was >2% in each layer ● bacI−A1 ● acI−A7

10 ●Iluma−A1

9

● acI−B1 ● Lhab−A1

Hypolimnion

8 7 ●CL500−11

6 5

● CL500−37

4

●CL500−3

3 2 1 0

●Iluma−A2

● CL500−15 LiUU−9−330 ● ● LD28 ● acIV−B ● acI−C2 ● Nitrosoarchaeum ●●●●● ●●●●● ● bacVI

●PnecB

●●●●● ●●●● ●LD2 ●acI−A1 ●LD19* ●●● ●Lhab−A2

613 OTUs ●●●●● ●●

0 Actinobacteria

1

Bacteroidetes

2

3

Alphaproteobacteria

4

● bacI ● acI−C2* ● acI−A4

5 6 Epilimnion

Betaproteobacteria

7

Planctomycetes

8 Chloroflexi

9

10

Verrucomicrobia

Others

(B) No. of lakes where the read proportion was >0.5% in each layer 10 9 Hypolimnion

8

● acI−C2 ● acIV−B

●plaI−A

●CL500−37 ● LiUU−9−330 ●CL500−3

●acI−C1

● CL500−11 ● LD19

7

●Lhab−A4

6

●CL500−15 ● bacVI* ● plaI−D

● bacIII−A

5

● plaI−E ● Opitutus

●Iluma−B1

4 3 2 1 0

● acI−A7 ●bacI−A1 ●Iluma−A1 ● acI−B1 ● Lhab−A1

●Iluma−A2 ● LD28

●● ●●

● ●● ●●

●PnecB

●Iluma−C1

● LD19*

● bacVI

●bacV

●●●●● bacI ●●●●● ● ● ●●● ●●●

● acI ●●

36 OTUs

● Cyanobacteria ● LD12 ●

●●●●● ●●●●● ● ●●● ●

491 OTUs 50 OTUs ●●●●

0 Actinobacteria

1

Bacteroidetes

2

3

Alphaproteobacteria

4

●Lhab−A2 ● acI−A1

● acI−A4 ● LD2

8

9

● acI−C2* ● Lhab−A2 ● bacI ● acTH1

5 6 Epilimnion

Betaproteobacteria

7

Planctomycetes

Chloroflexi

10

Verrucomicrobia

Others