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Biochemical Laboratory, Turkey. Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus. Abstract.
ORIGINAL RESEARCH Omur Tabak MD1 Remise Gelisgen PhD2 Hayriye Erman MD2 Fusun Erdenen MD1 Cüneyt Muderrisoglu MD1 Hale Aral PhD3 Hafize Uzun PhD2
Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus
1 Istanbul Education and Research Hospital,
Internal Medicine Clinic, İstanbul, Turkey 2 Department of Biochemistry, Istanbul University, Cerrahpasa Medical Faculty, İstanbul, Turkey 3 Istanbul Education and Research Hospital, Biochemical Laboratory, Turkey
Abstract Purpose: The purpose of this study was to determine the effects of diabetic complications on oxidation of proteins, lipids, and DNA and to investigate the relationship between oxidative damage markers and clinical parameters. Methods: The study group consisted of 69 type 2 diabetic patients (20 patients without complication, 49 patients with complication) who attended internal medicine outpatient clinics of Istanbul Education and Research Hospital and 19 healthy control subjects. In serum samples of both diabetic patients and healthy subjects, 8-hydroxy-2’deoxyguanosine (8-OHdG), as a marker of oxidative DNA damage, Nε-(hexanoyl)lysine (HEL) and 15F2t-iso-prostaglandin (15-F2t-IsoP). as products of lipooxidative damage, advanced oxidation protein products (AOPP), as markers of protein damage, and paraoxonase1 (PON1) as antioxidant were studied. Results: 15-F2t-IsoP (p