10 20 30 40 50 60 70 80 90 - PLOS

Download PDF
38 downloads 0 Views 339KB Size Report
Comment
PQFLRWFTEWAEE FCRIRK KLGKVKNECR--GETSGKRYCSGDGYDCTKTD ISRNI FYMDLDCPRCEEE CRKYDEWI ENKENELDKQKNKY - C.
A 10 20 30 40 50 60 70 80 90 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|... - he l i x 6 SD3 L o op - he l i x 7 XP_ 00 1 3 5 1 5 6 1 . 1 AAN35 6 0 5 . 1 AAN36 0 8 9 . 1 AAQ73 9 2 3 . 1 AAQ73 9 2 5 . 1 AAQ73 9 2 7 . 1 AAQ73 9 2 8 . 1 ABM88 7 5 3 . 1 ABM88 7 5 4 . 1 ABM88 7 8 0 . 1 ABM88 7 8 1 . 1 ABM88 7 8 2 . 1 ABM88 7 8 3 . 1 CAD20 8 6 7 . 1 CAD20 8 6 8 . 1 CAG25 1 7 3 . 1 ABM88 7 7 7 . 1 CAL 80 7 3 0 . 1 ACJ 68 3 2 8 . 1 PFDG_ 0 0 0 9 5 PFDG_ 0 0 1 7 5 PFDG_ 0 0 6 5 5 PFDG_ 0 0 7 0 1 PFDG_ 0 0 8 6 2 PFHG_ 0 3 2 3 4 PFHG_ 0 3 8 4 0 PFHG_ 0 5 0 5 2 XP_ 00 1 3 4 9 5 1 2 . 1 XP_ 00 1 3 4 9 5 1 3 . 1 XP_ 00 1 3 4 9 7 4 0 . 1 XP_ 00 1 3 5 1 3 2 1 . 1 XP_ 00 1 3 5 1 8 7 6 . 1

PQF LRWF E EWAEE F CR I KK I KLGKVKEACR - - DDSKKL - YCSHNGYDCTKT I RNKD I LSDNPKCTGCSVKCKVYE LWLRNQRNE F EKQKKKY PQF LRWF T EWAEE F CR I RK I KLGKVKNECR - - GE TSGKRYCSGDGYDCTKTD I SRN I F YMDLDCPRCE EECRKYDEW I ENKENELDKQKNKY PQF LRWF E EWAEE F CR I KK I KL ENVKNACR - - DEKKRK - YCSLNGFDCTQT I WKKKVFGRGNDCTNCSF KCF PYE I WLGNQREAFRKQKEKY PQF LRWFDEWAEE F CRRNK I K I DKTKE ECR - - GGNNGK - NCSREGYDCKRTDLGNN I I FMDL ECPNCADACKMYNRW I ENKKSE FNKQKQKY PQF LRWFNEWAEDF CR I KN I K I EHVTKTCT - - GESNNK - HCSREGYDCKRTDLKKNE I FMDLECPRCE E ECTSYNEWLKKKEGE FNKQKKKY PQF LRWFNEWAEE F CRVREHKLKK I KEACR - - GGNNKK - ECSKEGYDCKKTN I KRNE I F VDLDCPRCE EECTSYNEWLKKKEGE FNKQNNKY PQF LRWFDEWADDF CR I RNHKLQKVKEACR - - DERRGK - YCSLNGFDCTQT I WKKGVLHWSNECTDCSVKCNLYE I WLGNQREAFRKQKEKY PQYLRWFQEWAEDF CR I KK I KL E LAKKACR - - DESSKL - YCSHNGYDCTKR I EKGKSCSRDSKCTECSTKC I PYEYWLEKQQNE FNMQKDKY PQF LRWF E EWAEE F CRKKK I KLGNVKKACR - - DESSKL - YCSHNGYDCTQT I RNKD I C I RESKCTDCSTKCKLYE LWL EKQENE F KKQTKKY PQF LRWFNEWAEE F CR I RK I K I DKTKEEC I - - GENNGK - NCSREGYDCNKTNLKLNE I F VDLDCPRCEKACTSYNEW I ENKKKE F KKQKRKY PQYLRWF E EWAEDF CRKRN I KLKSAKNACR - - NESKQL - YCSHNGYDCTKR I EKGKSCSRDSKCT ECSTKC I PYEYWL EKQQNE FNMQKDKY PQF LRWFDEWSEDF CR I KK I KLGRVKKACR - - DESSKL - YCSHNGYDCTKT I RNKD I C I RESKCTGCLVKCNPYE I WLENQRKE FDKQKEKY PQF LRWYDEWAE E F CR I RK I K I EN I KKVCR - - DESNE I - YCSGDGHDCTQTNLSHNQ I F VDLDCPRCQDQC I KYNEW I VKKLE E F SKQKKKY PQYLRWFDEWGEE F CRKRN I KLKKVKDSCC - - NDKERL - YCCHDGYDCT TT I WKKGSLHLDNKCTDCL TKCKVF EVWLGNQQEAF KKQKEKY PQYLRWFDEWGEE F CRKRN I KLKKVKDSCR - - NYKERL - YCSHNGYDCTT T I WRKG I LHLDNKCTDCSTKCKVF EVWLGNQQEAF KKQKEKY PQF LRWFDEWSEE F CRKRN I TLKSAKEECQ - - NDSKKL - YCSLNGYNCTRL I PNKNYCSRDP I CTPCSNKC I PYDLWLRNRRDE FNMQKGKY PQF LRWYDEWADDF CR I RNHKLQKVKDTCQGYNNSGYR I YCSGDGEDCTN I LKQNFN I VSDF F CPSCKT ECTNYKKW I NKKQGE FNKQKKKY PQF LRWFDEWAEE F CR I RK I KL ENVKKECR - - DEPNNK - YCSGDGHDCKRTYLKDNT I F I DLNCPRCENACSNYTKW I E I QRKQFDKQKRKY PQF LRWF E EWSEE F CR I KK I K I DKVKKECR - - DEQNKK - YCSGDGHDCTQTNLSHNQ I F VDLDCPRCQDQC I KYNEW I VKKLE E F YKQNLKY PQF LRWF E EWAEE F CR I KK I KL ENVKKACR - - DESSKL - YCSHNGYDCTQT I RNKD I C I RESKCTDCSTKCKLYELWLEKQENE F KKQKQKY PQF LRWYDEWAEDF CR I RYHKLKR I KDACR - - NEKNGK - YCSHNGYDCRQMSWKKN I ESREHYCTGCF SACS I YNMWVDKQKKE F EKQKEKY PQYLRWFDEWAEDF CR I RKDKLKK I KNACR - - NYANNL - YCSFNGYDCTK I I WKEHNF SNDSKCTKCHNECLRYENW I KDQKLKF EKQRDKY PQF LRWFNEWAEE F CR I RK I KLGRVKKACR - - DESSKL - YCSHNGYDCTKT I RNKD I C I RESKCTDCSTKCKLYE I WLGNQRKE F EKQTKKY PQF LRWF E EWAEE F CR I RYHKLKR I KDACR - - NDSKKL - YCSHNGYDCTKTSWKKGVLHWSNECTDCSVKCKLYE I WLGNQREAFRKQKEKY PQYLRWFDEWSEE F CR I KN I K I GKVKNACR - - GEKHEK - DCSREGYDCKRTDLKKNE I FMDLECPNCADACKMYNRW I ENKKSE FNKQKQKY PQF LRWFNEWAEE F CR I RK I KL ENVKKACR - - DDSKKL - YCSHNGYDC I E T I RNED I LSDNPKCTGCSVKCKVYELWLRNQRNE F EKQKKKY PQF LRWFDEWADDF CR I KK I KL ENVKNACR - - DEKKRK - YCSLNGFDCTQT I WKKGVLHRSNECTGCLVKCNPYE I WLGNQREAFRKQKEKY PQF VRWF E EWADDF CR I RKHKLKN I KDVCR - - NESKPL - YCSLNGYDCTKTSWKNDF ESREHYCTPCF SACSLYK I WVANQKKE F EKQKKKY PQF LRWFDEWAEE F CR I KN I K I GN I KKSCT - - GESNNK - HCSREGYDCNKTNLRLNE I FMDL ECPRCADDCKSYE TWVENKKKE FNKQKEKY PQF LRWLNEWSE E F CR I KN I K I GN I KKSCT - - GESNNK - HCSREGYDCNKTNLRLNE I FMDL ECPRCADDCKSYE TWVEKKKKE FNKQKKKY PQF LRWFDEWAEE F CR I RNHKLRK I KDACR - - NDKERL - YCSQNGYDCTKR I EKGSSCSRENKCTGCSNKCVDYDFWL EKQQNE F K I QKDKY PQYLRWFDEWGEE F CRKRK I KL ENVEKACR - - KDSEKL - YCRHNGYDCTKT I KNEN I L FDDPKCTDCL I KCSLYE I WLDNQQKE F EKQKEKY -

cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys4 cys2 cys2 cys4 cys2 cys2 cys2 cys2 cys2 cys1 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2 cys2

AAA75 3 9 8 . 1 AAB60 2 5 1 . 1 AAC05 2 2 0 . 1 AAC05 7 3 0 . 1 AAF 18 9 8 0 . 1 AAK49 7 4 2 . 1 AAL 12 8 4 5 . 1 AAN35 6 0 4 . 1 AAN36 0 9 3 . 1 AAN36 2 7 5 . 1 AAN36 4 7 6 . 1 AAN36 4 7 7 . 1 AAN36 4 7 8 . 1 AAN36 6 1 5 . 1 AAO67 4 1 1 . 1 AAS89 2 5 9 . 1 ABM88 7 4 9 . 1 ABM88 7 5 1 . 1 ABM88 7 5 7 . 1 ABM88 7 5 8 . 1 ABM88 7 6 0 . 1 ABM88 7 6 1 . 1 ABM88 7 6 3 . 1 ABM88 7 6 7 . 1 ABM88 7 6 8 . 1 ABM88 7 6 9 . 1 ABM88 7 7 0 . 1 ABM88 7 7 1 . 1 ABM88 7 7 2 . 1 ABM88 7 7 4 . 1 ABM88 7 7 6 . 1 ACT 90 6 3 5 . 1 AEL 87 2 7 0 . 1 CAB39 1 1 5 . 2 CAG24 9 9 0 . 1 CAG25 1 3 7 . 1 CAG25 1 4 0 . 1 I T4 v a r 6 1 I T4 v a r 6 2 I T4 v a r 6 3 I T4 v a r 6 6 I T4 v a r 6 7 I T4 v a r 6 8 PFDG_ 0 0 0 7 2 PFDG_ 0 0 1 4 1 PFDG_ 0 0 2 5 9 PFDG_ 0 0 2 8 4 PFDG_ 0 0 3 5 3 PFDG_ 0 0 3 5 5 PFDG_ 0 0 3 5 7 PFDG_ 0 0 4 1 2 PFDG_ 0 0 6 0 4 PFDG_ 0 0 6 0 7 PFDG_ 0 1 6 5 2 PFDG_ 0 1 6 8 2 PFDG_ 0 1 7 4 3 PFDG_ 0 2 0 6 8 PFDG_ 0 2 3 7 2 PFDG_ 0 2 4 2 2 PFHG_ 0 0 5 9 2 PFHG_ 0 2 4 1 9 PFHG_ 0 2 4 2 1 PFHG_ 0 2 4 2 3 PFHG_ 0 2 4 2 5

PQYLRWF E EWAEDF CRKKKKYVD I VKTNCR - - NY - - SRNLYCSGNGLDCQE T I RV I GHHV I GSECSKCSVWCRRYKKW I DNQKEE F LKQKKKC PQYLRWF E EWAEDF CRKRKKK I ENA I KNCRGE - K - GNER - YCDLNGYNCEE TARGAE I F VKGDDCHKCSVACDRF VKW I DNQRKE FDKQKKKY PQF LRWF E EWAEDF CRLRKHKLKDA I QKCREKHK - GAKKLYCDLNRYDCEQTASGKHDF F EEDDCKGCQYSCARF VNW I DNQKKE F LKQKKKY PQYLRWF E EWAEDF CRKKKKKL ENL EKQCRGKD - KSDEYRYCSRNGYDCEQT I SRKGKVRMGKGCTDCF F ACHSYENW I DNQRKQFDKQKKYT PQYLRWF E EWAEDF CR I KQLKLQNVKNSCRG - EDD - - - NKYCSRNGYDCEQT I NK I GHRRFGNGC I KCL F ECNPYVKW I GNKEKDF EKQKKKC PQYLRWF E EWAEDF CRLRKHKL ENAKNKCRKPN - - - GVEKYCDLNRYDCEKTASGKHDF F EDDVCKDCQYSCAP F VDW I DNQKLE F LKQRNKY PQYLRWF E EWAEDF CTKRKHKLQNA I K I CRGDS - - - GNDRYCDLNGYDCTKTARGKNKRF SNDECYKCSLPCDHF VPW I DNQQKE F EKQKKKY PQYLRWF E EWAEDF CRKKKHKLKDA I DKCRGQYQ - D - ADRYCDLNGYDCEKTKRG I NMYRWDHKCTGCF LSCSHFRTW I DNQKEQFHKQKK I Y PQYLRWF E EWAE E F CR I KE LKL ENVKTRCRG - EKDG - - EKYCSRNGYDCTGT I I KRN I FRPDPECTNCL F ECNHYQDW I DNKKKE F EKQKKKC PQYLRWF E EWAEDF CRKKNKKLKDVKTNCRGDSG - - - TDRYCSGNGYDCTQT I RA I GKLV I GEHCTNCSVWCRLYEKW I DNQKLE F LKQRNKY PQYLRWF E EWAEDF CRKKKKKLPNVKTNCR - GEN - - NK - KYCSGDGFDCTKT I RAKY I YA I GDECTKCSFWCGF YKKWLENQKQE F LKQKKKY PQYLRWF E EWAEDF CRKKNKKLNDVKKQCR - GDN - - GTDRYCSGNGYDCTKT I RA I GKYA I GDECTKCSFWCRMYETW I DNQKKE F LKQKRKY PQYLRWF E EWAEDF CRKKK I YVG I VKKYCL - - - DE T E - EKYCSLNGCDCTQTVRAKGKLRYGNRCTDCL F ACHRYEKW I DNQRKQF LKQRNKY PQYLRWF E EWAEDF CRLRKHKLKDA I DKCRGGS - - - SNDKYCSGNGFDCVE TVRGDEHF VEK - DCHDCSYSCSP F VKW I DNQKLE LDKQKKKY PQYLRWFDEWT EDF CRKRKSQL ENA I QNCRGKDE - YGE EKYCSPNGHDCKT T I RAKD I YS I SKECAKCF F SCHRYVKW I DNQKLE F EKQKENY PQYLRWF E EWAEDF CRKKKKF LD I VKTNCR - GEN - - GSERYCSFNGYDCEKT I NK I DHLVMGNNCTKCSVACRLYQKW I ENQKQE F I KQKKKY PQYLRWLQEWAEDF CTKRKHKLKDA I QKCRGQYQ - - GKERYCDLNGYDCKGTARGKNKYKYEHDC I ECSF SC I P F TNW I DNQKLE F LKQKEKY PQYLRWF E EWAEDF CRKREYKL ENAKNKCRGNYE - NGEPKYCSRNGYDCKKTVRGKN I LVSDT ECTKCSVVCTP F VKW I DNQKEE F LKQKEKY PQYLRWF E EWAEDF CRKRKHKL ENA I QKCRYN - E - NGE EKYCSRNGYDCTRT I RGKNKL F SDT ECTNCSVVCTP F VDW I DNKKKE F EKQKGKY PQYLRWF E EWAEDF CRKKKKKL ENLDTQCRGTDA - SKEPRYCSLNGCDCTKTVRARGKLRYGNRCTDCL F ACNPYVHW I DKKKEE FNKQVKKY PQYLRWFDEWSEDF CRKKKKKL ENVQKQCRGKYGDDDT ERYCSRNGYDCEQTVNARGKLRYGKQC I SCLYACNPYVEW I DKQRKQFDKQKNRY PQF LRWF E EWSEDF CRKKNKK I KDVKRNCRGER - - - DGEKYCSRNGYDCEKTKRA I GKYHMGNQCTKCL F ACNPYVEW I DNQRKQFDKQVKKY PQYLRWF E EWAE E F CTKRKHKLQNA I TNCR - DY - - - AQNLYCSGNGYNCKE T I RAENKLVEGDDCHKCSVSCKP F VKW I DNQKLE F EKQKKKY PQYLRWF E EWAEDF CRKRKHKL ENA I KNCRGE - - - KGNERYCSRNGYDC I NTVR I QGKLVKGSECDDCYSSCTP F VKW I DDKKQE F LKQKKKY PQYLRWF E EWAEDF CGKKKKF LD I VKTNCR - GKN - - GTDRYCSFNGYDCEKT I NK I DHLVMGNNCTKCSVACRLYQKW I ENQKQE F I KQKKKY PQF LRWF E EWAEDF CRKKKKKL ENL EQQCRGEG - - - GKERYCSGNGYDCEKT I YKKGKLV I SSECTKCSVWCRMYETW I DNQKKE F I KQKRKY PQYLRWF E EWAEDF CRKRKRKL EDA I QKCRKPK - - - GQPKYCDLNRHDCVE T I RGNHVF F EEDDCKDCQYSCSHF VNW I DNQKLE F LKQKEKY PQYLRWF E EWAEDF CRKRKKKL ENA I KNCRY - DE - SGEPKYCDLNGYNCEE TARGRNKF APDSNCNKCSVACKP F VEWLDNQQKE F EKQKKKY PQYLRWF E EWAEDF CRKKKKYVN I VKK FCRE - - GENGE EKYCSRNGYDCTKTKPA I GKYRMGNQCTKCL F ACNPYVEW I EKQKEQFDKQKEKY PQYLRWF E EWAEDF CRKKKKKVENLQKQCRGQY - Q - GEKRYCSRNGCDCTKTVRAKGKLRYGKQC I SCLYACNPYVEW I DNQKEQFDKQKQRY PQF LRWF E EWAEDF CRLRKRKLKDA I QKCRGKDK - DGKKLYCDLNRYDCEKTASGKHDF F EEDDCKDCHF SCARF VKW I DNQKLE F LKQKQKY PQYLRWF E EWAEDF CRKKNKKVENVKKQCRKKDN - SSDDRYCSRNGYDCEKTKRA I GKLRYGKGCTECF F ACNPYVEW I EKQKEQFDKQKKKY PQYLRWF E EWAEDF CRKRKKQL ENAKKYCRGEDG - SGKELYCDLNRYDCKKT I SAEQKLVKGDDCNKCSVVC I P FGPW I DNQKLE F LKQKNKY PQYLRWF E EWAEDF CRLRKHKLQNAKNKCREKHK - DGKKLYCDLNGFDCTQTARGKNKYKYEHDC I ECYSSCDHF VHW I DNQKKE F EKQKNKY PQHLRWFDEWSEE F CRKRKDQLK I SL EKCRGKNK - D - - - KYCSLNGCNCKTTVRGKKKFDYQQECNDCLVACDP F VHW I DNEKLE F LKQKKKY PQYLRWF E EWAEDF CRKKK I KVENVKKSCRGEDK - ESKDRYCSRNGYDCEKTKRA I GKLRYGKQC I SCLYGCNPYVDW I EKQKEQFDKQKK I Y PQYLRWF E EWAEDF CRKKKKYVD I VKTNCR - GES - - GKDKYCSGDGFDCTKTVRAKG I YA I GDDCHKCSFWCGF YKKWLANQKQE F LKQKKKC PQF LRWF E EWAEDF CRKRKHKL ENA I DKCRGKN - - - GT ERYCDLNGYDCEQT I RGKNKLVEGEDCHKCTVPCDNF VHW I DNQQKE F EKQKKKY PQYLRWF E EWAEDF CRKKKKKL ENLQKQCRGQ - - FDKEPRYCSRNGYDCEKTKPA I GRLRMGKGCTDCF F ACNPYVEW I DNQRKQFDKQKKKY PQYLRWF E EWAEDF CRKKKKYVN I VKK FCREGEN - - GK - I YCSGDGFDCTKT I RA I G I YA I GDECTKCSVACRPYKRWLENQKQE F LKQKKKC PQYLRWF E EWAEDF CRKRKHKLKDV I EKCRGEN - - - GSERYCSGNGFDCKE TVRGDEHF VEK - DCHDCSYSCSP F VKW I DNQKLE F LKQRKKY PQYLRWF E EWAEDF CRKRKKK I ENA I KNCRY - DE - RGEPKYCDLNGFDCTKTASGEKKF VKGQDCHKCSVACNP F VDW I ENKKKE F EKQKNKY PQYLRWF E EWAEDF CRKKKKKVENLQKQCRGQN - DSGEPRYCSRNGYDCEKTKRA I GKLRYGKQC I SCLYACNPYVEW I EKQKEQFDKQVKKY PQYLRWF E EWAEDF CRKKNKK I KDVKRNCREKDK - NSE ERYCSRNGYDCEKTVRARGKLRYGKQC I SCLYGCNPYVEW I EKQKEQFDKQKNKY PQYLRWF E EWAEDF CRKRKHKL ENA I QKCRG - QY - QGKDRYCDLNGYDCEKTKRGRNKYRWDYKCTGCF LSCSHFRTW I DNQRKQF LKQKNKY PQYLRWF E EWAEDF CRKKKKKVENL EKSCRGEY - - QGADRYCSRNGYDCEKT I RK I E L LRMGKGCTDCLYACNPYVDW I ENQRKQF LKQKEKY PQF LRWFDEWAEDF CRLRKHKLQNAKEQCRGKN - - - GKEKYCDLNRYDCTQTASGEKKF VEDDVCKDCQYSCSHF VNW I DNQKLE F LKQKEKY PQF LRWF E EWGEE F CRLRKHKLQNA I NKCRYDEN - - KKERYCDLNGYDCKGTARGENKYKYEHDC I QCSF SCTP F TNW I DNQKEE F LKQKNRY PQYLRWF E EWSEDF CRKKKKYVN I VKTYCRG - EDKEGKERYCSRNGYDCEKTKRA I GKYRMGKQC I SCLYACNPYVEW I DNQRKQF LKQKKKY PQF LRWF E EWAEDF CRKKKKKVENLQKQCRGK - DKNDQERYCSRNGYDCEQTVNARGKLRYGKQCTDCLYACYPYVDW I DNQRKQFDKQKNKY PQHLRWF E EWAEDF CRKKKKKVENLQKQCRGQYQGAD - - RYCSRNGYDCEKTVNARGKLRYGNRC I DCLYACNPYVEW I DNQRKQF LKQKKKY PQYLRWF E EWGE E F CRLRKHKLQNA I KNCRGDSE - GGKKLYCDLNGFDCTKTAKGKNQLVEGEGCKKCTVPCDNF VHW I DNQKQE F EKQKNKY PQYLRWF E EWSEDF CRKKNKK I KDVKRNCRGPS - - - GTDKYCSRNGYDCTKTKRA I GKYRMGNQCTKCL F ACNPYVDW I NNQKEQFDKQVKKY PQF LRWF E EWAEDF CRKKKKKL ENL EKQCRGKYGDDGT ERYCSRNGFDCEKTVNARGKVRMGKGCTDCF F ACNPY I DW I DNQRKQFDKQKQKY PQYLRWF E EWAEDF CR I KQLKLKKL EKECRGKD - - - GKERYCSGNGYDCTKT I YKKGR I V I GSECTKCSVWCRMYESW I DNQKKE F LKQKRKY PQYLRWF E EWAEDF CRKKKHKL EDAKNKCRGTDS - SGKERYCDLNGYDCEKTKRGRNKYRWDYKCTGCF LSCSHFRTW I DNQRKQF LKQRNKY PQYLRWFDEWGEE F CRKRKKQL ENAKNKCRGYYE - NGQPKYCSLNGCNCKTTVRGKKKFDYEQECNDCLVACDP F I HW I DNQKLE F LKQKKKY PQHLRWFDEWSEE F CRKRKKQL ENAKKKCRGENN - D - - - KYCSLNGCDCKT TVREKKKFDYQRECNDCMVACDP F VQW I ENQE LE F LKQKKKY PQYLRWF E EWAEDF CRKKKQKL ENVQKQCRGEN - - - GKDKYCSRNGYDCEKTKRA I GKYRMGNQCTKCL F ACNPYVDW I DNQRKQFDKQVKKY PQYLRWFDEWT EE F CRKKKKYVD I VKKYCR - - - DESE - GKYCSLNGHDCT ET I RK I GL LRMGNGCTNCLHACTHYRGWLAKQQEE F EKQKKKY PQF LRWF E EWGEDF CRKKKKKL ENVQKQCRGEY - - QGE ERYCSRNGYDCEKTVNARGKVRMGKGCTDCF F ACNPY I DW I NNQRKQF LKQKERY PQF LRWF E EWGEDF CRKKKKKL ENVQKQCRGEYQ - - GE ERYCSRNGYDCEKTKRA I GKYRMGNQCTKCL F ACNPYVEW I DNQRKQFDKQKKKY PQYLRWF E EWAEDF CRKKKKYVD I VKTYCRG - KDKDGKKRYCSRNGFDCEKTKLA I GKLRYGKGCTDCF F ACNPYVHW I DNQRKQFDKQVKKY PQYLRWF E EWAEDF CRKRKHKL ENA I DKCRGDGK - GGKKLYCDLNGYNCERTKYK I GYF VEGADCKKCSVACKRF VEW I GKQKE E F LKQKQKY

cys4 cys5 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys2 cys4 cys4 cys4 cys4 cys2 cys4 cys4 cys3 cys4 cys4 cys4 cys4 cys4 cys4 cys5 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys2 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4 cys4

                                      10        20        30        40        50        60        70        80        90                                                ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|...  α­helix 6     SD3 Loop        α­helix 7      PFHG_02429                   PQYLRWLHEWAEDFCRKKNKKIKDVKRNCR­­GP­SGTDKYCSRNGYDCEKTVRARGKLRYGNRCIDCLYACNPYVHWIDKKKEEFDKQKKKY cys4  PFHG_03232                   PQFLRWFDEWAEDFCRKRKHKLQNAIKNCR­­TP­NGKDKYCDLNGYNCEETAKKENKLFPDSECHKCSVACNPFVPWIDNQKKEFEKQKGKY cys4  PFHG_03416                   PQYLRWFEEWAEDFCRKKKKKIPNVKTNCRQVKE­S­KEKYCTVGGYNCDGTIRKQYIYRLDTDCTKCSLACKTFAEWIDNQKQEFLKQKQKY cys4  PFHG_03480                   PQYLRWFEEWAEDFCRKKNKKIKDVQKQCRGTDS­SGKKRYCSRNGYDCEKTKRAIGKYRMGNQCISCLYGCNPYVEWIDNQRKQFDKQVKKY cys4  PFHG_03516                   PQYLRWFEEWAEDFCRKKNKKLEDAIKKCRGQDG­SGEKRYCDLNGYDCKRTFSAKKKYRWDHKCTGCFLSCSHFRTWIDNQKEQFLKQKNKY cys4  PFHG_04014                   PQFLRWFEEWAEDFCRKKAKKLQNAIKNCRGPN­­­GNDKYCSRNGYDCTKTKRAIGKYRMGNQCTKCLFACNPYVEWIDKKKEEFDKQVKKY cys2  PFHG_04015                   PQYLRWFDEWSEDFCRKKKKKLENVQKQCRGKYGDDDTERYCSGNGYDCERTIYKKGKLVIGYQCTNCSVLCRIYEKWIDNQKEQFLKQKGKY cys4  PFHG_04368                   PQYLRWFEEWAEDFCRKKKKKVENLEQQCRGKSEE­GEPRYCDRNGFDCERTKYKKGYFVIDKGCNTCSVWCRLYESWIDNQKKEFLKQKKKC cys4  PFHG_04491                   PQYLRWFEEWAEDFCRLRKHKLQNAKSKCRGEKN­D­­­KYCSRNGCDCTKTVRGKNKFSYEHECNDCLVACDPFVHWIDNQKLEFLKQKNKY cys4  PFHG_04593                   PQYLRWFDEWGEEFCRKRKKQLENAKKKCRGQDK­EGKERYCDLNGYDCEKTKRGRNMYRWDYKCTGCFLSCSHFRTWIDNQKEQFLKQRNKY cys4  PFHG_04749                   PQYLRWLQEWAEDFCRKKKIKVENVKKSCRGEN­­­GKDKYCSRNGYDCTKTKRAIGKLRYGNRCIDCLYACNPYVHWIDNQKEQFDKQVKKY cys4  PFHG_04770                   PQYLRWFEEWAEDFCRLRKHKLKDAIKKCRGDN­­­GTERYCDLNRHDCTKTASGKHDFFEGDDCIGCHFSCSHFVKWIDNQKLEFLKQKKKC cys4  PFHG_04910                   PQYLRWFQEWAEEFCRKKKKYVNIVKKYCR­­­DEKE­GKYCSLNGHDCTQTIRKIGLLRMGNECTKCLHACSHYRGWLANQKKEFEKQKEKY cys2  PFHG_05400                   PQYLRWFDEWTEEFCRLRRKKIEILEKICRG­KNDSGQPKYCSRNGCDCEQTINKIGRIRLGNGCTNCLFACNRYIDWINNKKKEFLKQKKKY cys4  XP_001347288.1               PQYLRWFEEWAEDFCRKKKIYVDIVKKYCR­­­GERDGEKYCSLNGCDCTKTVRARGKLRYGNRCTDCLFACHRYENWIDNQRKQFLKQRNKY cys5  XP_001347690.2               PQYLRWFEEWAEEFCRLRKHKLQNAIKNCRGEN­­­­NEKYCDLNGYDCEKTIRGKKKLFEGADCKKCTVTCDNFVPWIKNQKQEFDKQKNKY cys4  XP_001349030.1               PQYLRWFEEWAEDFCRKKKKKVQNLQKQCRDKY­­QGDDRYCSRNGYDCEQTINKIGKLVIGKGCINCLYACNPYVDWINNQKEQFDKQVKKY cys4  XP_001349031.1               PQYIRWFEEWAEDFCRKKKKKVQNLQKQCRGTD­ASKEPRYCSRNGFDCEKTISRIGKVRMGKGCTDCFFACHRYENWIDNQRKQFLKQKNIY cys4  XP_001349032.1               PQYLRWFEEWAEDFCRKKKKYVDIVKTYCRG­KYQ­GEERYCSRNGFDCEKTVNARGKVRMGKGCTDCFFACNPYVDWIDNQRKQFLKQRNKY cys4  XP_001349033.1               PQYLRWFEEWAEEFCRKRKKKLQNAQKFCR­­DE­SSK­LYCSLNGYDCKATIRANDEYTISQACAKCFFSCHRFVKWIDNQKLELEKQKKKY cys3  XP_001349034.1               PQYLRWFEEWAEDFCRKKKKYVDIVKTYCREQDKSGNQ­RYCSRNGYDCTKTVRARGKYRMGNQCTKCLFACNPYVEWIENQRKQFDKQVKKY cys5  XP_001349035.1               PQYLRWFEEWAEDFCRKRKKQLTDAIKNCREEDE­NGENRYCDLNGYDCKNTAKGKNKYKHDQECIKCSSVCIPFGPWIKNQKQEFEKQKNKY cys4  XP_001349036.1               PQYLRWFEEWAEDFCRKKKKYVDIVKKFCRE­­GENGKEKYCSLNGYDCTKTKLAVGKYRMGNQCTKCFFACYPYENWIEKQKEQFDKQKKIY cys4  XP_001349219.1               PQYLRWFEEWAEDFCRKKNKKIKDVKTNCRDE­­­­­KEKYCSGNGYDCRKTIYKKGKLVIGEHCTNCSVWCRLYEKWIDNQKKEFLKQKNKY cys4  XP_001349434.1               PQYLRWFEEWAEDFCRKRKHKLQNAKEQCRGKNG­EDK­­YCDLNGYDCKRTISAEKKLFPDSDCNKCSYSCIPFRTWIDNQKLEFLKQKNKY cys4  XP_001349437.1               PQYLRWFEEWGEDFCRKKKKKVENAKKKCRGENN­DK­­­YCSRNGCDCEKTVRAKGKLRYGNRCTDCLYACYPYVHWIDKKKEEFDKQVKKY cys4  XP_001349438.1               PQYLRWFEEWAEDFCRKKKHKLNDAIQKCRGEDK­YGKDRYCDLNGYDCEKTKRGRNMYRWDHKCTGCFLSCSHFRTWIDNQKLEFLKQKEKY cys4  XP_001349514.1               PQYLRWFEEWAEDFCRKRKYKLENAIEKCRG­KT­KGE­KYCDLNGFDCTQTASGEKKFVKGHNCHNCSVTCIPFGPWIDNQKKEFLKQRNKY cys4  XP_001349515.1               PQYLRWFEEWAEDFCRKKKKKIPNVKTNCRQVQR­G­KEKYCDRDGYNCDGTIRKQYIYRLDTDCTKCSLACKTFAEWIDNQKEQFDKQKQKY cys4  XP_001349737.2               PQYLRWFEEWAEDFCRKKKKKLEKLEQQCRDYK­­­­QNLYCSGNGYDCTKTIYKKGKLVIGEHCTNCSVWCRLYESWIDNQKLEFLKQKQKY cys4  XP_001350409.1               PQYLRWFEEWAEDFCRKKKKKLKDVKRNCRDE­­­­­TVKYCSGNGYDCTKTIYKKGKLVIGSECTKCSVWCRLYEKWIDNQKKEFLKQRNKY cys4  XP_001350936.1               PQYLRWFEEWAEDFCRLRKHKLKDAKNKCRGDS­­­GNDRYCDLNRYDCTQTIRGNEHFVEKDDCKGCQYSCAHFVNWIDNQKLEFEKQKEKY cys4  XP_001351079.1               PQFLRWFEEWAEDFCRLRKHKLKDAIDKCRTPK­­­GKEKYCDLNRYDCEQTIRGDHDFVEDDVCKGCQYSCSHFVNWIDNQKLEFDKQKRKY cys3  XP_001351318.1               PQYLRWFEEWTEEFCRKKKKKLEDVIKKCRYDEN­­NERKYCSRNGFDCKDTIRAQEKLVKGYDCHKCSVACDDFEPWIKNQKQEFEKQKGKY cys4  XP_001351319.1               PQYLRWFEEWAEDFCRLRKHKLKDAIKKCRGKN­­­GEEKYCDLNRYDCKNTASGKHVFFEDFDCKDCQYSCAPFVDWIDNQKLEFLKQRKKY cys4  XP_001351435.1               PQYLRWFEEWAEDFCRKKKKYVNIVKTYCRKK­DNSSEERYCSRNGFDCEKTKRAIGKLRYGKGCTDCFFACYPYEKWIDNKKKEFLKQKEKY cys4  XP_001351437.1               PQYLRWFEEWAEDFCRKKKIYVGIVKTYCREKYKSGNEPRYCSRNGYDCTKTKRAIGKYRMGNQCISCLYACNPYVDWINNQKEQFDKQKQKY cys4  XP_001351514.1               PQFLRWFEEWAEDFCRKKKKKLEKLEQQCRGVY­­EGKERYCSRNGFDCEKTVNARGKVRMGKGCTDCFFACNPYIDWIEKQKEQFDKQKQKY cys5  XP_001351515.1               PQYLRWFEEWAEDFCRKRKHKLKDVKTNCR­GEN­­GTDRYCSGNGLDCTKTIRAKYIYAIGSECTKCSFLCGFYKKWIDNQKKEFLKQKKKC cys4  XP_001351517.1               PQFLRWFEEWAEDFCRKKKKYVDIVKTYCREKDKY­GNERYCSGNGYDCTKTIYKKGRIVVGYECTSCSVWCRMYEKWIDNQKKEFLKQRNKY cys4  XP_001351563.1               PQYLRWFEEWAEDFCRKRKYKLENAKEQCREKDK­YYQERYCSGNGYDCTQTVRAQEEYSMENNCHKCFFACNPFVKWLGNQKLEFDKQKKKY cys4  XP_001351564.1               PQYLRWFEEWAEDFCRIRKHKLKNAKEQCREKYK­SGTDRYCSRNGYDCTQTIRGRNILVSDSECTNCSVVCTPFVKWIENKKLEFEKQKGKY cys4  XP_001351877.1               PQFLRWFEEWAEDFCRKKKHKLKDVKTNCR­ND­­­EEQRYCSGNGFDCTKTIYKKGRIVIGEHCTKCSVWCRMYESWIDNQKKEFLKQKRKY cys4  XP_001352242.1               PQYLRWFEEWAEDFCRKKKKYVDIVKTYCRG­KDKNSEERYCSRNGFDCEKTKRAIGKLRYGKQCISCLYACNPYVEWIDNQRKQFLKQKQKY cys4  XP_002808728.1               PQFLRWFEEWAEDFCRKKKKYVDIVKTYCR­­­­NETKGKYCSLNGYDCTKTKPAIGRLRMGNQCTKCFFACYPYEKWIDKKKEEFLKQKQKY cys4  XP_002808906.1               PQYLRWFEEWAEDFCRKKKKKVQNLEQQCRGQD­ISGNQRYCSRNGYDCEKTKPAIGRLRMGKGCTDCFFACNPYIDWINNQRKQFDKQKNKY cys4   

 

B 1 10 20 30 40 50 60 70 80 90 . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . .| . . . . | . . . . | . . . SD3 Group A P Q F L R W F x E W x E x F C R x x x x K L x x x K x x C x - - x x x x x x - - Y C S x x G Y D C x x x x x x x x x x x x x x x C x x C x x x C x x Y x x W xx x x x x x F x K Q K x K Y SD3 Group B P Q Y L R W F E E W A E D F C R K x K x K x x x x x x x C R x x x x x x x x x x Y C x x N G x D C x x T x x x x x x x x x x x x C x x C x x x C x x x x x W I D N Q K x x F x K Q K x KY

SD3 Group A 1 10 20 30 40 50 60 70 80 90 . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . .| . . . . | . . . . | . . . P Q F L R W F x E W x E x F C R x x x x K L x x x K x x C x - - x x x x x x - - Y C S x x G Y D C x x x x x x x x x x x x x x x C x x C x x x C x x Y x x W xx x x x x x F x K Q K x K Y

QP FLRWFDEWAEEFCR I KRK I KL Y

E

V

YN

L

S G

D

D

K

NH I G Y

R V NE D

T

K

E KN

RA D Q

S

KC

VI K A T

DE

E T

ES N T V

R

G

T

Q

C

I

E

G

GN

Y

N SR

E

E

C G DC

N KT I K T Y Y S K

D SNK L ND K K

I R

H

H

N

D E

E L R

G

C R

D

F H E

N

L

DL K

KN I F

I CSR NE WN DE QR D ME NR K S G LH SP D NR I I

I R

TL

E

G

RL NK E S S

M

I K

S

V

H R

V

F

D

L W

E

NH

Y

CC CYW TP

R D

G

N

E P

K

EN

ST A K

ED

AV N LE F

Q

E D

I L P

SS

T

D

V

NK

Q

I

E

L

FK

LI KNKQ E G

V

K R

D V D I

RK KE

QN

EG

RL

F KQK KY

E A

S K

Q

L

N

E

E K

R D

K

M I

ND Q T

R

N R

SD3 Group B 1 10 20 30 40 50 60 70 80 90 . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |. . . . | . . . . | . .. P Q Y L R W F E E W A E D F C R K x K x K x x x x x x x C R x x x x x x x x x x Y C x x N G x D C x x T x x x x x x x x x x x x C x x C x x x C x x x x x W I D N Q K x x F x K Q K x KY

P Q Y L R W F E E W A E D F CR F

H

L

D

Q

G S T

E

L

G T

CR Y C N D C T

KKRKKKLV NVAK G I

N

HY I I

Q

EK Q

D N

D

KK I TN

I LQ

E

Q

N

E D R

Q Y

KD YK

EE

S

G EKR K

ED

ND D D Q NE E N S TP Y DK G K G K

D GE D S K

EN E

NK

Q R L

QN

SDLR GY G GR D

F C

H

EK

N

T

K

Q E R

G T

I

R A K GK L R G

D K SG I N Y VM NE Y D VN R F

A YK P

I

H ER EH D Q V D

K

Y

I

I

F E A

KQ D

E

S

H

G

CC C

V

R

T KD

I HN

K

G

NS

SF A

VS L FY W

Q

L

V

R S

D

R

L

T

M

W I N F K QK K Y

Y D QK K E L Q F

PV

NH

K

E E D R I

K

H N T

P

E

L

N A

G

K

E

K K RL Q Q

D E

KN

RE VQ

I R

G

R

C