2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
Traders Hotel, Manila February 21-22, 2013
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
TABLE OF CONTENTS Message from the DOST Secretary Message from the DOST Undersecretary for R&D Message from the SEI Director Message from the ASTHRDP-NSC Steering Council Chairman About ASTHRDP and the NSC In Retrospect: ASTHRDP in 2012 Oral and Poster Presentation Sessions Plenary Speakers’ Profile
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ABSTRACTS OF TECHNICAL ORAL PRESENTATIONS
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Quorum Sensing Inhibition By Non-Polar Fractions from Halymenia Durvillaei Bory De Saint-Vincent From Bolinao, Pangasinan, Philippines, Espita, Dang Marviluz L./Montaño, Marco Nemesio, UPD
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Isolation, Characterization, and Molecular Cloning of A Novel Serine Protease-Like Gene of the Elastase Sub-Class from Moringa Oleifera Seed Embryos., Maini/ZA/Panes/VP/Canlas/MKC/RN, Garcia/VS, Tolentino, ADMU
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Synthesis, Morphological, and Structural Characterization of Moistureabsorber Para-Toluenesulfonic Acid-Doped Polyaniline, Bendoy, Anelyn P./Uy, Mylene M./Vequizo, Reynaldo M., MSU-IIT
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Isolation of High Molecular Weight Community DNA From Rumen Digesta for Metagenomic Studies, Sabinay, Stpehen G./Laurena, Antonio C./Sevilla, Cesar C./Yebron, Medino Gedeun N. Jr., UPLB
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Cyclic Diarylheptanoid Garugamblin-I Isolated From the Bark of Garuga Floribunda (“Bogo”), Pautong, Putlih Adzra T./Uy, Mylene M., MSU-IIT Removal of Organic and Inorganic Pollutants From Aqueous System By Chitosan Molecularly Imprinted Polymer in Modified and Unmodified Form, Templonuevo, Xyza Jane/Chakraborty, Soma, ADMU Functional Elucidation of Three Contigs of Marsupenaeus Japonicus to White Spot Syndrome Virus (WSSV) By dsRNA Inteference, Tare, Maria Violeta/Maningas, Mary Beth/Shitara, Aiko/Kondo, Hidehiro/Hirono, Ikuo, UST Taxonomy and New Distributions of invasive Suckermouth Armored Catfishes (Pisces: Pterygoplichthys), Chavez, Joel, DLSU Identification of Mangroves, Seagrasses and Seaweeds of Nogas Island, Anini-Y, Antique, Morillo-Manalo, Laureen/ Torrechilia-Guzman, Armi May, UPV Diversity of Some Endemic Nepenthes Species of the Philippines Based on Morphology, rbc L, mat K, and 18S rDNA Sequences, Acil, Rachel Y./Diaz, Ma. Genaleen Q./Amoroso, Victor B./Laude, Rita P./Mendioro, Merlyn S./ Laurena, Antonio C., UPLB Immunomodulatory and Chemopreventive Properties of Sulfated Polysaccharides from Sargassum Siliquosum J. G. Agardh, Vasquez, Ross D./Ramos, John Donnie, UST Gene Expressions of Pituitary Adenylate Cyclase Activating Polypeptide (tPACAP38)and Vitellogenin (Vtg) in Tilapia Exposed to De-71 and 17β- Estradiol Under Freshwater and Saline Water Conditions, Calma, Rosemarie R./ Heralde, Francisco M. III/Collazo, Margarita C./Schlenk, Daniel, DLSU Mcshane and Henstock Equi-integrability of Functions Taking Values in Locally Convex Topological Vector Space, Acapulco, Juniven P./Benitez, Julius V., MSU-IIT A Spectrally Negative Lévy Risk Process with Piecewise Constant Tax Structure, Veras, Kathleen Zoe E./Sy, Polly W./Valdez, Adrian Roy, UPD
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Construction and Structural Stability Analysis of Models of Dopamine D1 Receptor Trafficking in Renal Proximal Tubule Cells, Villar, John Justine S./Arceo, Carlene Perpetua P./Mendoza, Eduardo R., UPD
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S-Orbit Colourings of Square and Hexagonal, Raymundo, Gregorio Abaya III/Baraya-Walo, Ma. Lailani, UPD
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In the Complement of A Minimum Dominating Set in the Join, Corona and Composition of Graphs, Kiunisala, Edward M./Jamil, Ferdinand, MSU-IIT Dually-Weighted Stirling-Type Sequences, Gonzales, Ken M./Manuel Joseph C. Loquias/Corcino, Roberto B., UPD GIS-Based Assessment of Groundwater Vulnerability to Contamination in Boracay Island Using Drastic Model, Linan, Efren L./Ella, Victor B./Florece, Leonardo L., UPLB Zooplankton Composition and Abundance in Lamon Bay, Northeast Luzon, Philippines, Bollozos, Iris/Yniguez, Aletta T./Palermo, Joseph Dominic/Camoying, Marianne/Lopez, Mary Rose/Villanoy, Cesar, UPD
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
TABLE OF CONTENTS Response of the Juvenile Sea Cucumber Holothuria Scabra To Salinity Stress, Gorospe, Jay R C./Juinio-Meñez, Marie Antonette, UP-Diliman Growth Performance and Nutritional Efficiency of Silkworm (Bombyx Mori L.) Strains in Sericulture Center, University of the Philippines Los Baños, Laguna, Medillo, Japhia V./Ocampo, Virginia R., UPLB Mapping the Salinity Tolerance QTL of Hasawi At Seedling Stage Using F6 Recombinant inbred Lines Population,
Pascual, Evangeline D./Dela Viña, Celia B./Gregorio, Glenn B./Sajise, Andy/Amas, Junrey/Arceta, Marydee/ Barretto, Aniceta/Vispo, Aiza Naireen, UPLB Characteristics and Origin of Soils Along the Central Mountain Range in Leyte, Maranguit, Deejay S./Asio, Victor B., VSU Electrically Conducting Nis-Montmorillonite Nanocomposite Prepared By Ultrasonic Intercallation and Solid-Solid Reaction, Bermudez, Stephanie Ann O./Obiles, Romelyn Jane V./Cabañero, Joel M. Jr./Somintac, Armando B. , UP-Diliman Graphene / PEDOT-PSS Composites as Supercapacitor Electrode Materials, Chua, Stephanie L./Binag, Christina A., UST Incorporating Graphene in Anthocyanin Dye Sensitizer for Dye-Sensitized Solar Cells, San Esteban, Anna Carissa M./Enriquez, Erwin P., ADMU
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Fabrication and Characterization of Poly(Hydroxylauric Acid- e-Caprolactone) –Clay Nanofiber Composites: A New Potential Scaffold for Tissue Engineering, Monserate, Juvy J./Sumera, Florentino C., UP-Diliman
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Functionalization of Glass Substrates for DNA Sensing, Calupitan, Jan Patrick/Enriquez, Erwin P./Rojas, Nina Rosario L., ADMU
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Charge Pattern Writing on ZnO Microribbon Using AFM Electric Lithography, Permejo, Michael Francis P./ Singidas, Bess G./Vargas, Ray M./Sayson, Luce Vida/Sarmago, Roland V., UP-Diliman
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Ion Currents of Cu- Ion Beams in a Plasma Sputter Type Negative Ion Source and Its Correlation With Sputtering and Negative Ion Formation, Mahinay, Christian Lorenz S./Malapit, Giovanni M./Ulano, April M./Poral, Matthew D./Mascariñas, Venice F./Ramos, Henry J., UP-Diliman Complexity in Human Online Communication: Analysis and Modeling of Sending Patterns in e-Mail and Twitter, Solatorio, Aivin V./Lim, May T., UP-Diliman Characterization of Silica-Modified Polyaniline and Zinc Oxide Nanocomposites for Solar Cell Application, Rico, Christine Adelle L./Alguni, Arnold C./Vequizo, Reynaldo M., MSU-IIT Experimental Investigation of the Dynamics of a Granular, Hamoy, Efren/Confesor, Mark Nolan, MSU-IIT Isolation and Identification of Seaweed-Associated Carrageenase-Producing Bacteria, Tremedal, Paul Andrew/ David, Anne Marjorie E./Lluisma, Arturo O., UP-Diliman Degradation of Hydrocarbon By Halophilic Bacteria Isolated From Two Local Salterns in Luzon, Nanca, Carolyn L./ Dedeles, Gina Rio, UST An in Silico Analysis of the Effect of Drug Resistance On Virulence Genes of Entamoeba Histolytica, Palma, Juvy Ann C./Penuliar, Gil M., UP-Diliman Occurrence and Antimicrobial Activities of Fungal Endophytes From Philippine Mangroves, Moron, Llewelyn S./ dela Cruz, Thomas Edison E., UST Biodegradation of Untreated, Heat and UV-Treated Polyethylene and Oxo-Biodegradable Plastics By Bacteria Isolated From Soil, Vargas, Ma Christina B./Chan, Merab A., ADMU Safety And Bioefficacy Evaluation Of The Essential Oil From Pogostemon Cablin (Blanco) Benth. (Lamiaceae) Against Aedes Aegypti, Dacanay, Aleth Therese L./Osi, Marina O., UST Platelet – increasing Property of Euphorbia Hirta Linn. (Euphorbiaceae) in Thrombocytopenic Rat Models, Apostol, Jovencio G./Santiago, Librado A./Ysrael, Mafel Y., UST Cutaneous Bacteria of Frogs Collected From Mt Palay Palay Mataas Na Gulod National Park, Cavite for AntiChytridiomycosis Activity, Eda, Rae Abigail R./Diesmos, Arvin C./Chan, Merab A., ADMU Immunomodulatory and Anti-Scabies Properties of Tinospora Rumphii Boerl Lotion in Sarcoptes Scabiei Var Hominis -infected Patients and Its Predicted Shelf-Life : A Pilot Study, Castillo, Agnes L./Osi, Marina O./Ramos, John Donnie A./De Francia, Jean L./Dyjunco, Marylaine U./Quilala, Peter F., UST Antioxidant and Chemopreventive Properties of Sargassum Siliquosum and its Preformulation, Corpuz, Mary Jho-Anne T./Osi, Marina O./Santiago, Librado A., UST
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
TABLE OF CONTENTS ABSTRACTS OF TECHNICAL POSTER PRESENTATIONS 3D Reconstruction of Superhydrophobic Surfaces, Buendia, Johanna Evi P./Balili, Ryan B./Candidato, Rolando T., MSU-IIT
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A Biodiversity Report On the Myxomycete Assemblages in Leaf Litter, Twigs, Dungs, and Inflorescences, Buaya, Anthony T./dela Cruz, Thomas Edison E., UST Acceptability in Goats and Rumen in Situ Degradability of Foliage With Varying pH Levels From Selected Trees and Shrubs, Aban, Maita L./Bestil, Lolito C., VSU
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Analysis of Ethynylestradiol and Levonorgestrel in River Sludge, Enerio, Pio Gerard A./Dayrit, Fabian M., ADMU
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Analytical Calculation of the Radius of Gyration of a Polymer In Fractional Brownian Motion, Bantayan, Sim P./ Cabahug, Cresente O./Baybayon, Roel N./Arogancia, Dennis C./Gemao, Beverly V./Bornales, Jinky B ., MSU-IIT
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Biological Control of Pectinolytic, Fruit Soft Rot Agents Using Phylloplane Bacteria, Mateo, Bea C./Dedeles, Gina R., UST
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Biosorption On Cadmium (II) By Yeasts From Fruit Peels, Casamorin, Jovy Ann M./Dedeles, Gina R., UST
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Characteristics and Nutrient Status of Highly Weathered Soils in Silago, Southern Leyte, Piamonte, Maria Katrina B./Asio, Victor B., VSU Characterization of the Role of Fluctuations in Predator-Prey Dynamics Driven By Demographic Stochasticity, Palenzuela, Karlo/Juanico, Dranreb Earl, ADMU
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Decadal and Genre Comparison of Musical Networks From Midi Files, Valdez, Nicole/Cabatbat, Josephine Jill/ Roxas-Villanueva, Marianne/Tapang, Giovanni, UPD
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Describing Compartmentalization in the fore-Wing of Populations of the Rice Grasshoppers, Oxya sp. (Acrididae: Orthoptera), Coronel, Kimverly Hazel I./Torres, Mark Anthony J./Demayo, Cesar G., MSU-IIT Design of Electrostatic Ion Accelerator and Focusing System for the Plasma Sputter-Type Negative Ion Source (PSTNIS), Poral, Matthew D./Ramos, Henry V., UPD
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DNA Barcoding of the Philippine Traditional and Modern Rice Varieties, Uy, Mary Rose DL/Abella, Evaristo A./ Tabanao, Dindo Agustin A., CLSU
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Duadic Codes Over
, Florida, Veronica B./Dicuangco-Valdez, Lilibeth B./Betty, Rowena Alma L., UPD
Effects of Cultured Yeast Supplementation On Growing Kids Fed With Napier Grass-Concentrate Ration, Abela, Mark V./Bestil, Lolito C., VSU Effects of Substrate Orientation on the Growth of Zinc Sulfide Nanostructures, Odarve, Hananish Joy G./Alguno, Arnold C./Vequizo, Reynaldo M., MSU-IIT Efficiency of the Estimation of the Hurst Exponent in a Time Series Based on Frequency Domain Bootstrapping, Madronero, Jonathan/Evardone, Chita, MSU-IIT Ethnobotany of Some Pili (Canarium Ovatum) Varities Native To Gubat, Sorsogon, Chavez, Judith R./Cadiz, Nina M., UPLB Fabrication of Aluminum Nitride/Air-Gap Distributed Bragg Reflectors From Wet Etching of Sacrificial Aluminum Layers, Abat, Jonathan Ray R./Mangila, Nemesio S. IV, Salvador, Arnel A./Somintac, Armando S. , UPD Floristic Diversity and Avifaunal Composition in the Mangrove Ecosystem of Prieto Diaz, Sorsogon, Philippines , Labutap, Noel P./Gonzales-Geronimo, Rhia Odessa/Balatibat, Juancho B./Pampolina, Nelson M., UPLB NRC Fnesting Technique Using Regional Climate Model Version 3, Badrina, Robert B./Francisco, Raquel dV., UPD Gene Silencing of Vp9 Gene Impairs WSSV Infectivity on Macrobrachium Rosenbergii, Alenton, Rod Russel R./ Maningas, Mary Beth B., UST Growth and Characterization of Emeraldine Salt Polyaniline (PANi) Film On Silicon (n-Si) Wafer Substrate By Chemical Polymerization for Diode Application, Alcantara, Patrick Alvin M./Sambo, Bianca Rae B./Vequizo, Reynaldo M., MSU-IIT Heavy Metal Cadmium in Feeds and Tissues of Female Domestic Mallard (Anas Platyrhynchos Domesticus L.) in Victoria, Laguna and Candaba, Pampanga, Philippines, Octura, Josh Elisha R./Vega, Renato S.A./Sevilla, Cesar C./Tirol, Ma. Stella C./Capitan, Severino S./Lambio, Angel, UPLB In Situ Rumen Degradability of Varying Qualities of Basal Diets With Jackfruit By-Product and Soybean Meal Supplementation in Cattle, Zamora, Fatima A./Bestil, Lolito C., VSU
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
TABLE OF CONTENTS In Vitro Screening of the Vasorelaxant Activity of Diospyros Discolor Willd. (Ebenaceae) Leaf Extracts, Cadiang, Renz K. G./Osi, Marina O./Apostol, Jovencio G./Ysrael, Mafel C., UST Invasion Biology of Suckermouth Armored Catfishes (Pisces: Pterygoplichthys) , Chavez, Joel M./Carandang, Jose Santos R. VI, DLSU Isolation and Characterization of the Oil Bodies and Oleosins From the Seeds of Moringa Oleifera Lam., Uy, Abigail Loren T./Panes, Vivian A., ADMU
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Landmark-Based Geometric Morphometric Analysis On Bodyshape Variation of Mesopristes Cancellatus (Cuvier, 1829), Barazona, Christina A./Torres, Mark Anthony J./Gorospe, Jessie G./Demayo, Cesar G. , MSU-IIT
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Magnetic Field Dependence of the Transition Width of Bi2Sr2-Xmnxcacu2Oy Superconducting Films, Bambao, Leonalyn V./Sarmago, Roland V., UPD Mechanized Dry Direct Seeded Rice Social Enterprise Upholding Farmer Fair Trade, Gabunada, Lourd Franz M., UPLB Mineral Resources in the Philippines: Nickel Laterites, Ong, Russel M./Arcilla, Carlo A., UPD
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Norm-Preserving inverse Best Approximation in Hilbert Spaces, Orge, Karlo S./Artes, Rosalio G. Jr., MSU-IIT
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Occurrence of Fungal Foliar Diseases On Selected Mangrove Species in Oil-Spilled Areas in Taklong Island National Marine Reserve, Nueva Valencia, Guimaras, Philippines, Panaguiton, Diana Dalino/Sadaba, Resurreccion B., UPV
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Pathogenic Yeast Sterilization Via Microwave Atmospheric Pen Plasma (APP), Soriano, Joey Kim T./Del Rosario,
Leo Mendel D./Lacdan, Ma. Camille C./Lee, Henry V. Jr./Ting, Julie Anne S./Ramos, Henry J./Siringan, Maria Auxilia T./Tumlos, Roy B., UPD Performance and Meat Qualities of Broiler Fed Diets With Different Levels of Jackfruit (Artocarpus Heterophyllus, L.) Seed Meal, Gerebise, Ma. Rosa De Lima C./Taveros, Alberto A., VSU Performance of Growing Lambs As influenced By Liquid Acid Whey Supplementation, Poliquit, Angie R./Sanchez, Serena L., VSU Phenotypic and Genetic Characterization of Half-Sib Progenies of Three Eucalyptus (Eucalyptus Deglupta X E. Pellita) Hybrids in Northern Mindanao, Philippines, Piñon, Albert A./Carandang, Wilfredo M./Fernando, Edwino S,/Nuevo, Cesar C./Castillo, Manuel L./Galang, Marco A./Quimado, Marilyn O., UPLB Population Dynamics and Diversity of insects in Pummelo Grown Under Organic and Conventional Pest Management Systems, Aceres, Larry V./Ceniza, M.J.C./Noriel, L.M./Vasquez, E.A./Gonzaga, Z.C., VSU Relative Warp and Correlation Analysis Based On Distances of the Morphological Shell Shape Patterns of Pomacea Canaliculata Lamarck From Japan and the Philippines, Moneva, Carlo Stephen O./Torres, Mark Anthony J./ Wada, Takashi/Joshi, Ravindra/Demayo, Cesar G., MSU-IIT Screening of Actinomycete Isolates for Plant Growth-Promoting Activities (GPA), Cruz, Jayvee A./Paterno, Erlinda S., UPLB Seasonal Variability of Meiofaunal Community in A Seagrass Bed in Southern Guimaras, Philippines, Burgos, Lorelie A./del Norte-Campos, Annabelle, UPV Sexual Dimorphism in Modularity and integration in the fore and Hind Wing of the Common Evening Brown Butterfly Melanitisleda Linnaeus 1758 (Nymphalidae: Lepidoptera), Tumang, Vanessa Mae C./Torres, Mark Anthony J./Demayo, Cesar G., MSU-IIT Simulation Study of the Filtered Back Projection Method for Proton Computed Tomography of Bone Tissue, Perez, Ronn Marr M./Nawang, Salasa A., MSU-IIT Situation Analysis of Solid Waste Management at A Local High School in Region IV: A Benchmark for Establishing Solid Waste Management Plans in Public High Schools in the Southern Tagalog Region, Pasion, Daryl C/Erasga, Dennis S., DLSU
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Structural and Superconducting Effect of Doping Manganese On Strontium-Site of Bi2Sr-2Cacu2O8+δ, Santiago,
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The Flory Index of Fractional Brownian Polymer Using Monte Carlo Simulation, Baybayona, Roel N./Cabahug, Cresente O./Bantayan, Sim P./Bornales, Jinky B./Gemao, Beverly B./Arogancia, Dennis A., MSU-IIT
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Using GIS as Tool Towards a Climate-Disaster Resilient Community: Flood Risk Mapping in Marikina City, Philippines, Marquez, Teodorico L. Jr./Pulhin, Perlyn M., UPLB Vomifoliol, A Megastigmane Sesquiterpene From the Leaves of Psychotria Gitingensis Elmer, Eusebio, Jameson A./ Alejandro, Grecebio Jonathan D./Tan, Mario A., UST Yield and Nutrient Uptake of Lowland Rice As Affected By Application of Chicken Manure Under Different Water Management Systems, Ratilla, Melanie D./Escinas, Alfredo B., VSU
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
DEPARTMENT OF SCIENCE AND TECHNOLOGY Office of the Secretary
My warmest greetings to the organizers and participants of the 2nd National DOST-SEI ASTHRDP-NSC Scholar’ Conference. This conference serves as a good venue by which our graduate scholars can present their research outputs to the scientific and academic communities, primarily showcasing their potentials as future researchers and technologists. With the theme, “Innovations to Accelerate S&T Human Resource Development”, this event is expected to be an intellectually stimulating and socially rewarding experience. The presence of our eminent guest speakers and S&T experts will surely inspire our participants to actively discuss their research findings. The Department of Science and Technology (DOST) continues to affirm its commitment of supporting and providing a proper environment that will enhance and develop the S&T competitiveness of our nation’s most vital asset - its human capital. Science and Technology is associated with modernization and is an essential tool for a country’s rapid economic progress. It is the centerpiece of any nation’s development. With the products of science and technology, people’s lives become comfortable. This is made possible because of the significant contributions and ingenuity of individuals in the field of science and technology. It is hoped that through this annual gathering of graduate scholars in the various fields of science, engineering and technology, with the able guidance of their advisers and mentors, our ultimate goal of developing a culture for research and public appreciation of Science and Technology will come unto full measure. To the participants, may this conference motivate you to pursue your invaluable work in the field of scientific research and bring forth innovative ideas for practical applications that will result to a better quality of life of our people. May you always aspire for excellence and the improvement of your capabilities in your chosen field of expertise. Congratulations to all the presenters! Mabuhay tayong lahat!
MARIO G. MONTEJO Secretary
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
DEPARTMENT OF SCIENCE AND TECHNOLOGY Office of the Undersecretary for R&D
My warmest congratulations to the Science Education Institute (SEI) for holding the 2nd National DOST-SEI Accelerated Science and Technology Human Resource Development Program—National Science Consortium (ASTHRDP-NSC) Scholars’ Conference. This activity shows that we value the hardwork and achievements of our scholars. It shows further that the DOST remains very supportive of programs/projects aimed at building the nation’s capacity to uplift the status of science and technology as well as research and development in the country. Kudos to all the members of the organizing committee for the success of this endeavor. Be assured that we shall work hand-in-hand with you as we continue to develop the future leaders of our beloved country. Keep up the good work!
AMELIA P. GUEVARA, Ph.D. Undersecretary for Research &Development
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
SCIENCE EDUCATION INSTITUTE Office of the Director
My warmest greetings to the members of the National Science Consortium and the participants of this 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference In its 2nd serving, this conference hopes to spawn a healthier convergence among our much valued graduate scholars as we encourage them to go into research and development (R&D) that shall lead into innovations that would cater to the needs of our industries. The dynamic nature of science gives constant pressure to our industries as to how to adapt to the rapid advancements in technology and the production process. It is therefore critical that we create a sound scientific environment where R&D is at its crest in order to ensure the stable rise of the economy. We are of the firm belief that the participants of this conference, as evidenced by the kind of works presented in this conference are competent enough to influence the creation of such type of scientific environment. This conference is one of the many ways that the Science Education Institute supports to create a critical mass of scientists and engineers that will lead the country in finding S&T-based solutions to the pressing issues of this time. We hope that through this conference, we are able to do that and more. I congratulate all our participants and look forward to seeing you as active members of the science community. Thank you and mabuhay!
FILMA G. BRAWNER, Ph.D. Director
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
NATIONAL SCIENCE CONSORTIUM
February 12, 2013 On behalf of the Steering Committee of the National Science Consortium, I warmly welcome all the faculty, students and guests to the 2nd ASTHRD-NSC Student Research Conference! In 2010, the Science Education Institute convened the 10 top science universities in the country into the National Science Consortium (NSC) to assist it to implement the Accelerated Science & Technology Human Resource Development Program (ASTHRD) to fast-track the PhD and MS science scholarship programs of the country. The institutions which were invited into the NSC accepted this challenge. The NSC is a large and strategic consortium. All combined, the NSC has more than 700 PhD faculty and offers 33 PhD and 51 MS science degree programs. Currently, there are about 500 MS and 100 PhD ASTHRD scholars in the NSC. This is a heavy responsibility, and also a great opportunity to contribute to the development of the country. Beyond the management of the scholars, the NSC has taken the challenge to enhance our science programs in three ways: by improving of the training of our scholars, by promoting innovation in research, and by forming collaborative programs in strategic areas. I am pleased to announce that the DOST has responded positively by providing financial resources so that we can undertake our plans. This conference will feature two important concerns of the NSC: how to build an effective consortium and mentorship. Together, these two concerns are keys to a successful NSC. We are thankful to have with us the best resource speakers on these topics. Rev. Bienvenido F. Nebres, S.J. and Dr. Ester A. Garcia were two of the pillars of the first DOST science consortium; Dr. Lourdes Cruz, Dr. Oscar Zamora, and Dr. Florencia Claveria are exemplars of effective mentors in three areas of science. Their messages are addressed to both the faculty and scholars of the NSC. Of course, the main participants of this conference are the scholars. Unlike the usual conferences, this is an opportunity to share one’s work, test one’s ideas, and, yes, also to practice and improve. Secondly, this conference is also a unique opportunity for the NSC to meet and form shared arrangements, whether it is to teach and develop courses or to undertake collaborative research. It is our hope that this 2nd Student Research Conference, and its future editions, will become one of the foundations for a strong Filipino science community! Sincerely yours,
FABIAN M. DAYRIT, Ph.D. Chair, NSC Steering Council
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ABOUT ASTHRDP The Accelerated Science and Technology Human Resource Development Program (ASTHRDP) is a graduate program being implemented by the Department of Science and Technology (DOST) through the Science Education Institute and the Councils. The main objective of the program is to help improve the country’s global competitiveness and capability to innovate through S&T and to accelerate the production of high-level human resources needed for research and development in S&T.
ABOUT NSC The National Science Consortium (NSC) is a consortium of ten (10) member-universities, namely the ADMU, CLSU, DLSU, MSU-IIT, UP-Diliman, UP-Los Baños, UP-Manila, UP-Visayas, UST and VSU. The NSC was developed to support and coordinate national programs to accelerate the development of high level human resources in basic, applied sciences and mathematic for the country’s sustainability and competitiveness.
ASTHRDP ADVISORY COMMITTEE
NATIONAL SCIENCE CONSORTIUM STEERING COUNCIL
Chair:
Dr. FABIAN M. DAYRIT Professor, Department of Chemistry, ADMU
Vice-Chair:
Dr. SOLEDAD M. ROGUEL Vice President for Academic Affairs, CLSU
Chair:
Engr. MARIO G. MONTEJO Secretary, DOST
Members:
Dr. AMELIA P. GUEVARA Undersecretary for R&D, DOST
Dr. ARLENE A. PASCASIO Dean, College of Science, DLSU Dr. EVELYN C. CREENCIA Dean, College of Science and Mathematics, MSU-IIT
Members:
Dr. FILMA G. BRAWNER Director, SEI-DOST
Dr. JOSE MARIA P. BALMACEDA Dean, College of Science, UP-Diliman
Dr. ROWENA CRISTINA L. GUEVARA Executive Director, PCIEERD
Dr. JOSE V. CAMACHO, Jr. Dean, Graduate School, UP-Los Baños
Dr. JAIME C. MONTOYA Executive Director, PCHRD
Dr. NINA G. GLORIANI Dean, College of Public Health, UP-Manila
Dr. PATRICIO S. FAYLON Executive Director, PCAARRD
Dr. MA. LUISA E. MABUNAY Dean, College of Arts and Sciences, UP-Visayas
Dr. FABIAN M. DAYRIT Chair, ASTHRDP-NSC Steering Council
Dr. MARIBEL G. NONATO Assistant Rector for Research and Innovation, UST Dr. VICTOR B. ASIO Dean, College of Agriculture, VSU
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
IN RETROSPECT: ASTHRDP IN 2012
1st National DOST-ASTHRDP Scholars’ Conference February 2012, College of St. Benilde Hotel, Manila
ASTHRDP Orientation and Signing of the Scholarship Agreement July 2012, DOST, Bicutan, Taguig City
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
IN RETROSPECT: ASTHRDP IN 2012 In Touch with Excellence July 2012, Hyatt Hotel, Manila
ASTHRDP-NSC Meeting May 2012, Guimaras
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ORAL PRESENTATION SESSION 1 Venue: Time
Embassy Ballroom 1 Session
Name
1:30 - 1:50
Espita, Dang Marviluz L./Montaño, Marco S01-1 Nemesio
1:50 - 2:10
S01-2
2:10 - 2:30 S01-3
Title Quorum Sensing Inhibition By Non-Polar Fractions from Halymenia Durvillaei Bory De Saint-Vincent From Bolinao, Pangasinan, Philippines
Maini/ZA/Panes/VP/Canlas/MKC/RN, Garcia/ Isolation, Characterization, and Molecular Cloning of A Novel Serine Protease-Like VS, Tolentino Gene of the Elastase Sub-Class from Moringa Oleifera Seed Embryos. Bendoy, Anelyn P./Uy, Mylene M./Vequizo, Reynaldo M.
School UPD ADMU
Synthesis, Morphological, and Structural Characterization of Moistureabsorber Para MSU-IIT -Toluenesulfonic Acid-Doped Polyaniline
Sabinay, Stpehen G./Laurena, Antonio C./ 2:30 - 2:50 S01-4 Sevilla, Cesar C./Yebron, Medino Gedeun N. Jr.
Isolation of High Molecular Weight Community DNA From Rumen Digesta for Metagenomic Studies
2:50 - 3:10 S01-5 Pautong, Putlih Adzra T./Uy, Mylene M.
Cyclic Diarylheptanoid Garugamblin-I Isolated From the Bark of Garuga Floribunda (“Bogo”)
MSU-IIT
3:10 - 3:30 S01-6 Templonuevo, Xyza Jane/Chakraborty, Soma
Removal of Organic and Inorganic Pollutants From Aqueous System By Chitosan Molecularly Imprinted Polymer in Modified and Unmodified Form
ADMU
Venue:
Embassy Ballroom 2
Time
Session
1:30 - 1:50
S02-1
Name Tare, Maria Violeta/Maningas, Mary Beth/ Shitara, Aiko/Kondo, Hidehiro/Hirono, Ikuo
1:50 - 2:10 S02-2 Chavez, Joel Morillo-Manalo, Laureen/TorrechiliaGuzman, Armi May Acil, Rachel Y./Diaz, Ma. Genaleen Q./ 2:30 - 2:50 S02-4 Amoroso, Victor B./Laude, Rita P./Mendioro, Merlyn S./Laurena, Antonio C. 2:10 - 2:30 S02-3
2:50 - 3:10 S02-5 Vasquez, Ross D./Ramos, John Donnie
3:10 - 3:30 S02-6
Venue: Time 1:30 - 1:50
Calma, Rosemarie R./Heralde, Francisco M. III/Collazo, Margarita C./Schlenk, Daniel
Title Functional Elucidation of Three Contigs of Marsupenaeus Japonicus to White Spot Syndrome Virus (WSSV) By dsRNA Inteference
Session
Name
S03-1 Acapulco, Juniven P./Benitez, Julius V.
2:50 - 3:10 S03-5 Kiunisala, Edward M./Jamil, Ferdinand
UST
Taxonomy and New Distributions of invasive Suckermouth Armored Catfishes (Pisces: Pterygoplichthys)
DLSU
Identification of Mangroves, Seagrasses and Seaweeds of Nogas Island, Anini-Y, Antique
UPV
Diversity of Some Endemic Nepenthes Species of the Philippines Based on Morphology, rbc L, mat K, and 18S rDNA Sequences
UPLB
Immunomodulatory and Chemopreventive Properties of Sulfated Polysaccharides from Sargassum Siliquosum J. G. Agardh
UST
Gene Expressions of Pituitary Adenylate Cyclase Activating Polypeptide (tPACAP38) and Vitellogenin (Vtg) in Tilapia Exposed to De-71 and 17β- Estradiol Under Freshwater and Saline Water Conditions
Title Mcshane and Henstock Equi-integrability of Functions Taking Values in Locally Convex Topological Vector Space
DLSU
Carlota Session S04-1
Name Linan, Efren L./Ella, Victor B./Florece, Leonardo L.
Bollozos, Iris/Yniguez, Aletta T./Palermo, 1:50 - 2:10 S04-2 Joseph Dominic/Camoying, Marianne/Lopez, Mary Rose/Villanoy, Cesar 2:10 - 2:30 S04-3
Gorospe, Jay R C./Juinio-Meñez, Marie Antonette
2:30 - 2:50 S04-4 Medillo, Japhia V./Ocampo, Virginia R.
School MSU-IIT
A Spectrally Negative Lévy Risk Process with Piecewise Constant Tax Structure
UPD
Construction and Structural Stability Analysis of Models of Dopamine D1 Receptor Trafficking in Renal Proximal Tubule Cells
UPD
S-Orbit Colourings of Square and Hexagonal
UPD
In the Complement of A Minimum Dominating Set in the Join, Corona and Composition of Graphs
3:10 - 3:30 S03-6 Gonzales, Ken M./Manuel Joseph C. Loquias/ Dually-Weighted Stirling-Type Sequences
1:30 - 1:50
School
Ambassador Sala
Veras, Kathleen Zoe E./Sy, Polly W./Valdez, 1:50 - 2:10 S03-2 Adrian Roy Villar, John Justine S./Arceo, Carlene Perpetua 2:10 - 2:30 S03-3 P./Mendoza, Eduardo R. Raymundo, Gregorio Abaya III/Baraya-Walo, 2:30 - 2:50 S03-4 Ma. Lailani
Venue: Time
UPLB
Title GIS-Based Assessment of Groundwater Vulnerability to Contamination in Boracay Island Using Drastic Model
MSU-IIT UPD
School UPLB
Zooplankton Composition and Abundance in Lamon Bay, Northeast Luzon, Philippines
UPD
Response of the Juvenile Sea Cucumber Holothuria Scabra To Salinity Stress
UPD
Growth Performance and Nutritional Efficiency of Silkworm (Bombyx Mori L.) Strains in Sericulture Center, University of the Philippines Los Baños, Laguna
UPLB
Pascual, Evangeline D./Dela Viña, Celia B./ Gregorio, Glenn B./Sajise, Andy/Amas, Junrey/ Mapping the Salinity Tolerance QTL of Hasawi At Seedling Stage Using F6 Arceta, Marydee/Barretto, Aniceta/Vispo, Aiza Recombinant inbred Lines Population Naireen 3:10 - 3:30 S04-6 Maranguit, Deejay S./Asio, Victor B. Characteristics and Origin of Soils Along the Central Mountain Range in Leyte
2:50 - 3:10 S04-5
16
UPLB VSU
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ORAL PRESENTATION SESSION 2 Venue: Time
Embassy Ballroom 1 Session
Name
Title
School
Bermudez, Stephanie Ann O./Obiles, Romelyn Electrically Conducting Nis-Montmorillonite Nanocomposite Prepared By 4:00 - 4:20 S05-1 Jane V./Cabañero, Joel M. Jr./Somintac, Ultrasonic Intercallation and Solid-Solid Reaction Armando B.
UPD
4:20 - 4:40 S05-2 Chua, Stephanie L./Binag, Christina A.
UST
Graphene / PEDOT-PSS Composites as Supercapacitor Electrode Materials
San Esteban, Anna Carissa M./Enriquez, Erwin Incorporating Graphene in Anthocyanin Dye Sensitizer for Dye-Sensitized Solar 4:40 - 5:00 S05-3 P. Cells 5:00 - 5:20 S05-4 Monserate, Juvy J./Sumera, Florentino C. 5:20 - 5:40 S05-5
Venue: Time
Calupitan, Jan Patrick/Enriquez, Erwin P./ Rojas, Nina Rosario L.
Fabrication and Characterization of Poly(Hydroxylauric Acid- e-Caprolactone) – Clay Nanofiber Composites: A New Potential Scaffold for Tissue Engineering Functionalization of Glass Substrates for DNA Sensing
ADMU UPD ADMU
Embassy Ballroom 2 Session
Name
Title
School
Permejo, Michael Francis P./Singidas, Bess G./ 4:00 - 4:20 S06-1 Vargas, Ray M./Sayson, Luce Vida/Sarmago, Charge Pattern Writing on ZnO Microribbon Using AFM Electric Lithography Roland V.
UPD
Mahinay, Christian Lorenz S./Malapit, 4:20 - 4:40 S06-2 Giovanni M./Ulano, April M./Poral, Matthew D./Mascariñas, Venice F./Ramos, Henry J.
Ion Currents of Cu- Ion Beams in a Plasma Sputter Type Negative Ion Source and Its Correlation With Sputtering and Negative Ion Formation
UPD
4:40 - 5:00 S06-3 Solatorio, Aivin V./Lim, May T.
Complexity in Human Online Communication: Analysis and Modeling of Sending Patterns in e-Mail and Twitter
UPD
5:00 - 5:20 S06-4
Rico, Christine Adelle L./Alguni, Arnold C./ Vequizo, Reynaldo M.
5:20 - 5:40 S06-5 Hamoy, Efren/Confesor, Mark Nolan
Venue: Time
Characterization of Silica-Modified Polyaniline and Zinc Oxide Nanocomposites for Solar Cell Application
MSU-IIT
Experimental Investigation of the Dynamics of a Granular
MSU-IIT
Ambassador Sala Session
Name
Title
Tremedal, Paul Andrew/David, Anne Marjorie 4:00 - 4:20 S07-1 Isolation and Identification of Seaweed-Associated Carrageenase-Producing Bacteria E./Lluisma, Arturo O.
School UPD
4:20 - 4:40 S07-2 Nanca, Carolyn L./Dedeles, Gina Rio
Degradation of Hydrocarbon By Halophilic Bacteria Isolated From Two Local Salterns in Luzon
UST
4:40 - 5:00 S07-3 Palma, Juvy Ann C./Penuliar, Gil M.
An in Silico Analysis of the Effect of Drug Resistance On Virulence Genes of Entamoeba Histolytica
UPD
5:00 - 5:20 S07-4
Moron, Llewelyn S./dela Cruz, Thomas Edison Occurrence and Antimicrobial Activities of Fungal Endophytes From Philippine E. Mangroves
5:20 - 5:40 S07-5 Vargas, Ma Christina B./Chan, Merab A.
Venue: Time
Carlota Session
Name
4:00 - 4:20 S08-1 Dacanay, Aleth Therese L./Osi, Marina O. Apostol, Jovencio G./Santiago, Librado A./ 4:20 - 4:40 S08-2 Ysrael, Mafel Y. 4:40 - 5:00 S08-3
Eda, Rae Abigail R./Diesmos, Arvin C./Chan, Merab A.
Biodegradation of Untreated, Heat and UV-Treated Polyethylene and OxoBiodegradable Plastics By Bacteria Isolated From Soil
Title Safety And Bioefficacy Evaluation Of The Essential Oil From Pogostemon Cablin (Blanco) Benth. (Lamiaceae) Against Aedes Aegypti Platelet – increasing Property of Euphorbia Hirta Linn. (Euphorbiaceae) in Thrombocytopenic Rat Models Cutaneous Bacteria of Frogs Collected From Mt Palay Palay Mataas Na Gulod National Park, Cavite for Anti-Chytridiomycosis Activity
Castillo, Agnes L./Osi, Marina O./Ramos, John Immunomodulatory and Anti-Scabies Properties of Tinospora Rumphii Boerl Lotion in 5:00 - 5:20 S08-4 Donnie A./De Francia, Jean L./Dyjunco, Sarcoptes Scabiei Var Hominis-infected Patients and Its Predicted Shelf-Life : A Pilot Marylaine U./Quilala, Peter F. Study 5:20 - 5:40 S08-5
Corpuz, Mary Jho-Anne T./Osi, Marina O./ Santiago, Librado A.
Antioxidant and Chemopreventive Properties of Sargassum Siliquosum and its Preformulation
17
UST ADMU
School UST UST ADMU
UST
UST
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
POSTER PRESENTATIONS 1 2
3D Reconstruction of Superhydrophobic Surfaces, Buendia, Johanna Evi P./Balili, Ryan B./Candidato, Rolando T. A Biodiversity Report On the Myxomycete Assemblages in Leaf Litter, Twigs, Dungs, and Inflorescences, Buaya, Anthony
T./dela Cruz, Thomas Edison E.
MSU-IIT UST
3
Acceptability in Goats and Rumen in Situ Degradability of Foliage With Varying pH Levels From Selected Trees and Shrubs, Aban, Maita L./Bestil, Lolito C.
4
Analysis of Ethynylestradiol and Levonorgestrel in River Sludge, Enerio, Pio Gerard A./Dayrit, Fabian M.
5
Analytical Calculation of the Radius of Gyration of a Polymer In Fractional Brownian Motion, Bantayan, Sim P./Cabahug, Cresente O./Baybayon, Roel N./Arogancia, Dennis C./Gemao, Beverly V./Bornales, Jinky B.
6
Biological Control of Pectinolytic, Fruit Soft Rot Agents Using Phylloplane Bacteria, Mateo, Bea C./Dedeles, Gina R.
UST
7
Biosorption On Cadmium (II) By Yeasts From Fruit Peels, Casamorin, Jovy Ann M./Dedeles, Gina R.
UST
8 9 10
Characteristics and Nutrient Status of Highly Weathered Soils in Silago, Southern Leyte, Piamonte, Maria Katrina B./Asio,
Victor B. Characterization of the Role of Fluctuations in Predator-Prey Dynamics Driven By Demographic Stochasticity, Palenzuela,
Karlo/Juanico, Dranreb Earl Decadal and Genre Comparison of Musical Networks From Midi Files, Valdez, Nicole/Cabatbat, Josephine Jill/Roxas-
Villanueva, Marianne/Tapang, Giovanni
11
Describing Compartmentalization in the fore-Wing of Populations of the Rice Grasshoppers, Oxya sp. (Acrididae: Orthoptera), Coronel, Kimverly Hazel I./Torres, Mark Anthony J./Demayo, Cesar G.
12
Design of Electrostatic Ion Accelerator and Focusing System for the Plasma Sputter-Type Negative Ion Source (PSTNIS), Poral, Matthew D./Ramos, Henry V.
13
DNA Barcoding of the Philippine Traditional and Modern Rice Varieties, Uy, Mary Rose DL/Abella, Evaristo A./Tabanao,
Dindo Agustin A.
14 Duadic Codes Over 15 16 17
, Florida, Veronica B./Dicuangco-Valdez, Lilibeth B./Betty, Rowena Alma L.
Effects of Cultured Yeast Supplementation On Growing Kids Fed With Napier Grass-Concentrate Ration, Abela, Mark V./
Bestil, Lolito C. Effects of Substrate Orientation on the Growth of Zinc Sulfide Nanostructures, Odarve, Hananish Joy G./Alguno, Arnold
C./Vequizo, Reynaldo M. Efficiency of the Estimation of the Hurst Exponent in a Time Series Based on Frequency Domain Bootstrapping,
Madronero, Jonathan/Evardone, Chita
18 Ethnobotany of Some Pili (Canarium Ovatum) Varities Native To Gubat, Sorsogon, Chavez, Judith R./Cadiz, Nina M. 19 20 21 22 23
Fabrication of Aluminum Nitride/Air-Gap Distributed Bragg Reflectors From Wet Etching of Sacrificial Aluminum Layers,
Abat, Jonathan Ray R./Mangila, Nemesio S. IV, Salvador, Arnel A./Somintac, Armando S. Floristic Diversity and Avifaunal Composition in the Mangrove Ecosystem of Prieto Diaz, Sorsogon, Philippines , Labutap,
Noel P./Gonzales-Geronimo, Rhia Odessa/Balatibat, Juancho B./Pampolina, Nelson M. Fnesting Technique Using Regional Climate Model Version 3, Badrina, Robert B./Francisco, Raquel dV. Gene Silencing of Vp9 Gene Impairs WSSV Infectivity on Macrobrachium Rosenbergii, Alenton, Rod Russel R./Maningas,
Mary Beth B. Growth and Characterization of Emeraldine Salt Polyaniline (PANi) Film On Silicon (n-Si) Wafer Substrate By Chemical Polymerization for Diode Application, Alcantara, Patrick Alvin M./Sambo, Bianca Rae B./Vequizo, Reynaldo M.
Heavy Metal Cadmium in Feeds and Tissues of Female Domestic Mallard (Anas Platyrhynchos Domesticus L.) in Victoria, 24 Laguna and Candaba, Pampanga, Philippines, Octura, Josh Elisha R./Vega, Renato S.A./Sevilla, Cesar C./Tirol, Ma. Stella
VSU ADMU MSU-IIT
VSU ADMU UPD MSU-IIT UPD CLSU UPD VSU MSU-IIT MSU-IIT UPLB UPD UPLB UPD UST MSU-IIT
UPLB
C./Capitan, Severino S./Lambio, Angel 25
In Situ Rumen Degradability of Varying Qualities of Basal Diets With Jackfruit By-Product and Soybean Meal Supplementation in Cattle, Zamora, Fatima A./Bestil, Lolito C.
18
VSU
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
POSTER PRESENTATIONS 26 27 28 29 30 31
In Vitro Screening of the Vasorelaxant Activity of Diospyros Discolor Willd. (Ebenaceae) Leaf Extracts, Cadiang, Renz K. G./Osi,
Marina O./Apostol, Jovencio G./Ysrael, Mafel C. Invasion Biology of Suckermouth Armored Catfishes (Pisces: Pterygoplichthys) , Chavez, Joel M./Carandang, Jose Santos R.
VI Isolation and Characterization of the Oil Bodies and Oleosins From the Seeds of Moringa Oleifera Lam., Uy, Abigail Loren T./
Panes, Vivian A. Landmark-Based Geometric Morphometric Analysis On Bodyshape Variation of Mesopristes Cancellatus (Cuvier, 1829),
Barazona, Christina A./Torres, Mark Anthony J./Gorospe, Jessie G./Demayo, Cesar G. Magnetic Field Dependence of the Transition Width of Bi2Sr2-Xmnxcacu2Oy Superconducting Films, Bambao, Leonalyn
V./Sarmago, Roland V. Mechanized Dry Direct Seeded Rice Social Enterprise Upholding Farmer Fair Trade, Gabunada, Lourd Franz M.
32 Mineral Resources in the Philippines: Nickel Laterites, Ong, Russel M./Arcilla, Carlo A. 33 Norm-Preserving inverse Best Approximation in Hilbert Spaces, Orge, Karlo S./Artes, Rosalio G. Jr. 34
35
36 37
Occurrence of Fungal Foliar Diseases On Selected Mangrove Species in Oil-Spilled Areas in Taklong Island National Marine Reserve, Nueva Valencia, Guimaras, Philippines, Panaguiton, Diana Dalino/Sadaba, Resurreccion B. Pathogenic Yeast Sterilization Via Microwave Atmospheric Pen Plasma (APP), Soriano, Joey Kim T./Del Rosario, Leo
Mendel D./Lacdan, Ma. Camille C./Lee, Henry V. Jr./Ting, Julie Anne S./Ramos, Henry J./Siringan, Maria Auxilia T./ Performance and Meat Qualities of Broiler Fed Diets With Different Levels of Jackfruit (Artocarpus Heterophyllus, L.) Seed Meal, Gerebise, Ma. Rosa De Lima C./Taveros, Alberto A. Performance of Growing Lambs As influenced By Liquid Acid Whey Supplementation, Poliquit, Angie R./Sanchez, Serena
L.
Phenotypic and Genetic Characterization of Half-Sib Progenies of Three Eucalyptus (Eucalyptus Deglupta X E. Pellita) Hybrids 38 in Northern Mindanao, Philippines, Piñon, Albert A./Carandang, Wilfredo M./Fernando, Edwino S,/Nuevo, Cesar C./
UST DLSU ADMU MSU-IIT UPD UPLB UPD MSU-IIT UPV
UPD
VSU VSU UPLB
Castillo, Manuel L./Galang, Marco A./Quimado, Marilyn O. 39
Population Dynamics and Diversity of insects in Pummelo Grown Under Organic and Conventional Pest Management Systems, Aceres, Larry V./Ceniza, M.J.C./Noriel, L.M./Vasquez, E.A./Gonzaga, Z.C.
Relative Warp and Correlation Analysis Based On Distances of the Morphological Shell Shape Patterns of Pomacea 40 Canaliculata Lamarck From Japan and the Philippines, Moneva, Carlo Stephen O./Torres, Mark Anthony J./Wada,
VSU
MSU-IIT
Takashi/Joshi, Ravindra/Demayo, Cesar G. 41 42 43 44 45 46 47
48 49
50
Screening of Actinomycete Isolates for Plant Growth-Promoting Activities (GPA), Cruz, Jayvee A./Paterno, Erlinda S. Seasonal Variability of Meiofaunal Community in A Seagrass Bed in Southern Guimaras, Philippines, Burgos, Lorelie A./
del Norte-Campos, Annabelle Sexual Dimorphism in Modularity and integration in the fore and Hind Wing of the Common Evening Brown Butterfly Melanitisleda Linnaeus 1758 (Nymphalidae: Lepidoptera), Tumang, Vanessa Mae C./Torres, Mark Anthony J./Demayo, Cesar G. Simulation Study of the Filtered Back Projection Method for Proton Computed Tomography of Bone Tissue, Perez, Ronn
Marr M./Nawang, Salasa A. Situation Analysis of Solid Waste Management at A Local High School in Region IV: A Benchmark for Establishing Solid Waste Management Plans in Public High Schools in the Southern Tagalog Region, Pasion, Daryl C/Erasga, Dennis S. Structural and Superconducting Effect of Doping Manganese On Strontium-Site of Bi2Sr-2Cacu2O8+δ, Santiago, Alvin
Carl M./Sarmago, Roland V. The Flory Index of Fractional Brownian Polymer Using Monte Carlo Simulation, Baybayona, Roel N./Cabahug, Cresente
O./Bantayan, Sim P./Bornales, Jinky B./Gemao, Beverly B./Arogancia, Dennis A. Using GIS as Tool Towards a Climate-Disaster Resilient Community: Flood Risk Mapping in Marikina City, Philippines,
Marquez, Teodorico L. Jr./Pulhin, Perlyn M. Vomifoliol, A Megastigmane Sesquiterpene From the Leaves of Psychotria Gitingensis Elmer, Eusebio, Jameson A./Alejandro,
Grecebio Jonathan D./Tan, Mario A. Yield and Nutrient Uptake of Lowland Rice As Affected By Application of Chicken Manure Under Different Water Management Systems, Ratilla, Melanie D./Escinas, Alfredo B.
19
UPLB UPV MSU-IIT MSU-IIT DLSU UPD MSU-IIT
UPLB UST
VSU
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
PLENARY SPEAKERS’ PROFILE
Dr. Ester A. Garcia Dr. Ester Albano Garcia has been the President of the University of the East (UE) from 2006 to present and concurrent member of the UE and UERMMMC Board of Trustees and Steering Committee, South and Southeast Asia and Taiwan Universities Presidents’ Forum. She finished her bachelor’s degree in Chemistry at the University of the Philippines Diliman, Cum Laude and PhD degree at the Ohio State University. After which, she has been an international research fellow of the ICN Nucleic Acid Research Institute in California and Syracuse University. She was a Professor of the University of the Philippines from 1970 to 2003 and became the Professorial Lecturer of the same university from 2003 to 2006. She considers her being a faculty member of the University of the Philippines as her most important achievement in her professional career. She has published 20 scientific papers in peer-reviewed journals of national and international circulation, and has written at least 130 other papers on various issues related to science, education, technology, environment and gender. Dr. Garcia helped organize two (2) government agencies: the Philippine Council for Advanced Science and Technology Research and Development (PCASTRD) under the DOST and Commission on Higher Education (CHED). She initiated scholarship programs in these agencies. She was the Deputy Executive Director of the DOST-PCASTRD in 1988 and later on became a Commissioner of the Commission on Higher Education (CHED) in 1994 and Chair of the Commission in 1999. Moreover, Dr. Garcia conceptualized major development projects for CHED, particularly on institutional strengthening funded by ADB and the strengthening of the whole higher education sector funded under the Higher Education Development Fund and Mindanao Advanced Education Project and other faculty development programs funded by CHED. The Engineering and Science Education Project, a World Bank-funded DOST project which supported science and engineering colleges all over the country was initiated by Dr. Garcia during her years of service in the PCASTRD. Among the numerous prestigious awards she received were: The National Research Council of the Philippines Achievement Award in Chemistry; Philippines Federation of Chemistry Societies Achievement Award in Chemical Education; Professional Regulations Commission Chemistry Professional of the Year in 1996; Government Service Award of the Ateneo De Manila University in 2003; Doctor of Laws, Honoris Causa of the De La Salle University; Outstanding Women Chemists of the Philippines during the International Year of Chemistry, 26th Philippine Chemistry Congress in 2011; and Award of Highest Honor at the Soka Women’s College, Japan, in March 2012.
Fr. Bienvenido F. Nebres, S.J. Fr. Bienvenido F. Nebres, S.J. served as President of the Ateneo de Manila University for 18 years 1993-2011. He was Dean of the Ateneo College of Arts and Sciences 1973-1980. He was Provincial Superior of the Jesuits in the Philippines 1983-89 during the time of democratic transition to President Corazon Aquino after the years of martial law under President Marcos. He was also President of Xavier University in Cagayan de Oro in the early 1990s. He did extensive work to develop science and mathematics in the Philippines and in Southeast Asia and was elected in 2011 as National Scientist by the National Academy of Science and Technology (NAST). He was conferred the award by President Benigno Aquino III in September 2011. After retirement as President, he continues as Professor of Mathematics at the Ateneo de Manila and is engaged in various programs to help overcome poverty in the country: improving public education in over 400 public schools with the Ateneo Center for Educational Development (ACED) and in over 200 municipalities, particularly in the Autonomous Region of Muslim Mindanao, with Synergeia Foundation; building homes and communities for the poor with Gawad Kalinga; and supporting young people engaged in social enterprises for poor communities. Fr. Nebres did his early studies in classics and philosophy in the Jesuit Scholasticate, Berchmans College, and completed an AB and MA in philosophy. He obtained a PhD in mathematics from Stanford University.
20
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
PLENARY SPEAKERS’ PROFILE Dr. Lourdes J. Cruz Dr. Lourdes J. Cruz is a National Scientist and a Professor Emeritus at the Marine Science Institute, University of the Philippines-Diliman (UPD). She finished her baccalaureate degree in Chemistry at the UPD in 1962 and pursued her MS and PhD degree in Biochemistry at the University of Iowa, Iowa City, U.S.A. Dr. Cruz is concurrent President of the Center for BioMolecular Science Foundation and the Bataan Center for Innovative S&T, Inc. She was a President of the National Research Council of the in 2012. She has been a professor of the University of the Philippines-Manila, College of Medicine in 1970; part-time Research Professor of the University of Utah, Department of Biology in 1987. She has held various positions in research institutions and the academe. Because of her achievements as a researcher and educator, she received international and local awards such as: Sven Brohult Award, International Foundation for Science, Sweden in 1993; Outstanding ASEAN Scientist and Technologist Award, Brunei Darrusalam in 2001; L’Oreal-UNESCO Award For Women In Science, Asia Pacific Region (First Filipino & ASEAN to win the award), Paris, France in 2010; Distinguished Alumnus Award, University of Iowa (Roy & Lucille Carver College of Medicine), Iowa City, Iowa, USA in 2011; Outstanding Young Scientists Award (Chemistry), NAST in 1981; Achievement Award, NRCP in 1982; The Outstanding Women in the Nation's Service (Science) TOWNS Foundation and Lion's Club (Philippine Chapter) in 1986; and Academician, National Academy of Science and Technology (NAST) in 1987 among others. She was conferred the Order of National Scientist (Presidential Proclamation No. 1167, signed on 10 Nov 2006) in 2008. In 2012, she was the Representative of the Philippines in the International Council of Scientific Unions, Regional Committee for Asia and the Pacific and a Fellow of the Third World Academy of Science. To date, she has acted as adviser to 9 PhD, 17 MS and 42 BS graduates. She has published a total of 137 papers on different research works in her field of specialization and hold patents for 12 of her works.
Dr. Evelyn Mae T. Mendoza Dr. Evelyn Mae Tecson-Mendoza, a member of the National Academy of Science and Technology (NAST) Philippines since 1992 , is a retired Research Professor and UP Scientist III at the Institute of Plant Breeding, College of Agriculture, University of the Philippines Los Baños (UPLB). She headed the Crop Biotechnology Program of the Crop Science Cluster, and was chair of both the BS Agricultural Biotechnology and UPLB Molecular Biology and Biotechnology Program Management Committees. She holds a BS in Chemistry cum laude (Gold Medalist) from the Mapua Institute of Technology and an MS and PhD in Biochemistry from the University of Massachusetts Amherst. She has worked extensively on biochemical aspects of Philippine agricultural and underexploited crops published in more than 100 technical papers in refereed journals. Her more recent researches are on developing transgenic papaya with long shelf life and virus resistance, protein engineering of mungbean storage proteins and molecular studies of coconut storage and oil body proteins. A recipient of several national, professional and institutional awards, she has served as editor of three scientific journals. She was the Editor-in-Chief of the Philippine Agricultural Scientist (2004-2009) and the Philippine Journal of Crop Science (1983-1987; 1990-2000) and has been an Overseas Editor of the Bioscience, Biotechnology and Biochemistry since 2007. With colleagues, she led the development and institution of the MS and PhD Molecular Biology and Biotechnology Program and the BS Agricultural Biotechnology Program at UPLB.
Dr. Florencia G. Claveria Dr. Florencia G. Claveria is a full time professor of the Biology Department, College of Science, De La Salle University-Manila. She obtained her BS in Biology at the St. Louis University in 1973 and MS in Zoology in Ghent State University, Belgium in 1975. She finished her PhD in Biological Science at the University of Cincinnati, Ohio, USA in 1987. She has published 67 research papers in manuscripts and publications. Her research interests are Protozoan zoonotic infections and their vectors (Babesia, Cryptosporidium, Toxoplasma, Sarcocystis, among others (immunological and parasitological aspects); Parasitic infections/helminths in farm animals; and Developmental Biology and Avian infections. Dr. Claveria was a Visiting Professor of the National Research Center for Protozoan Diseases in Obihiro University, Hokkaido, Japan; Visiting Lecturer of Health Sciences, Henan University, China in 2003; and Post-Doctoral Fellow of the Department of Veterinary Physiology and Protozoan Immunology, Obihiro University, Hokkaido Japan in 1990. She is currently the president of the Philippine Society of Parasitology, Inc. She is also a full member of the National Research Council of the Philippines (NRCP); member of the Governing Board, Center for Tropical Biology, Bogor, Indonesia, SEAMEO BIOTROP, Editorial Board, Philippine Journal of Science, STII, Department of Science and Technology, Manila; Chair, Technical Committee of Biology and Molecular Biology, Commission on Higher Education (CHED); and Member, Technical Panel of Science and Mathematics, Commission on Higher Education TPSM (CHED). She is a recipient of various awards and distinctions: Grantee, Fulbright-Hays, Ph. D. Biological Sciences, University of Cincinnati, Ohio, USA (September 1982 to August 1987); Most Outstanding Ph.D. Student, Department of Biological Sciences, University of Cincinnati in 1987; Grantee, Government of Belgium, M. S. Zoology, Ghent State University (September 1973 to October 1975); St. Miguel Febres Cordero Award, DLSU-Manila in 2004; Outstanding Teacher Award, College of Science, DLSU-Manila in 1995, among others.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
PLENARY SPEAKERS’ PROFILE Dr. Oscar B. Zamora Dr. Oscar B. Zamora is currently a Professor 12 of the Department of Crop Science and Vice Chancellor for Instruction of the University of the Philippines-Los Baños (UPLB). He graduated with a degree in BS Agricultural Chemistry and MS Agronomy at UPLB. He finished his PhD in Crop Physiology/Agricultural Systems at the University of Queensland, Australia. He is a member of nine honor/professional societies. He attended, participated and served as resource person, organizer/co-organizer, facilitator and technical coordinator in at least 75 national and international conferences/workshops/trainings/meetings in the Philippines, Belgium, Brazil, Cambodia, Democratic People's Republic of Korea, Germany, India, Indonesia, Italy, Japan, Malaysia, Singapore, Spain, Sweden, Switzerland, Thailand, The Netherlands and Vietnam. Dr. Zamora is the Member of the Philippine delegation to the Second Conference of Parties of the Convention on Biological Diversity (COP) held at Jakarta, Indonesia in 1995 and the 4th FAO International Technical Conference on Plant Genetic Resources for Food and Agriculture (PGRFA) held at Leipzig, Germany. He is also the Visiting Professor of the Katholieke Universiteit Leuven, Belgium and University of Waggeningen University, Netherlands. He is an Editor in Chief of the Journal of Environmental Science and Management since 2006. His long years of experience in research and development work on sustainable/alternative agriculture, alternative crop breeding strategy, organic farming, genetic resources conservation, media development and environment concern in collaboration students, NGO and colleagues in the academe, he has published as sole author or co-author 22 technical papers in refereed journals; eight magazine articles on sustainable agriculture, participatory rural development, farmer-scientist partnership, IPR and sui generis system, genetic engineering and food security; and more than 80 papers presented in national and international conferences (published as proceedings) on topics such as sustainable agriculture (conceptual, curriculum development and indicators), on-farm conservation of plant genetic resources, biotechnology/genetic engineering, biosafety, IPR and sui generis system, farmers’ rights and benefit sharing, participatory development, farmer-scientist partnership for development, food security issues, globalization and liberalization. Dr. Zamora was a member of the NSC Steering Council representing UPLB from 2010 to May 2012.
Dr. Ludwig Streit Ludwig P. A. E. Streit is an expert in quantum field theory, quantum mechanics, nuclear physics, stochastic processes and infinite dimensional analysis, analysis of complex networks and polymer physics. He finished his education in Doctor of Philosophy from the Univ. Graz, Austria with distinction in 1962. Dr. Streit has been a “Honorarprofessor” of Graz University in 1972 and Agregação at the Univ. do Minho, Portugal in 1988. In his professional career, he has held various positions in the academe in different countries as well as in research laboratories. Dr. Streit was a professor of the Physics Department of the Syracuse University, University Bielefeld in Germany and Universidade do Minho. He was appointed as the Director of various offices such as Center for Interdisciplinary Research, BiBoS Research Center for Stochastics and Applications in Universidade da Madeira and Centro de Ciências Matemáticas in 1995. Dr. Streit was a member of the IUPAP Committee for Mathematical Physics in 1981, a member of the Executive Committee International Association for Mathematical Physics (IAMP) in 1979 and later on became the Secretary of the IAMP in 1982. He has also served as consultant in many notable projects, one close to our heart, is his membership in the Scientific Advisory Committee of the Research Center for Theoretical Physics in Bohol. As an authority in his areas of specialization, he was involved various editorial activities from 1973 up to 2002. He has also extended visits to numerous research institutions and universities, and currently, he is a Visiting Professor at the Mindanao State UniversityIligan Institute of Technology. He has mentored research students and fellows from France, India, Malaysia, Nigeria, Austria, Poland, Portugal, Tunisia, Ukraine and the Philippines. He has also served as referee for scientific journals, books, fellowship, research projects and hiring committees.
Mr. Andrew Michael S. Ong Mr. Ong is the former Deputy Director General for Legal, Policy and International Relations of the Intellectual Property Office of the Philippines. His fixed term appointment ended on December 31, 2012. He is an architect by formal training, having completed his Bachelor of Science in Architecture from the University of San Carlos in Cebu and topping the Government National Licensure Examination in 1986, while he practices law, after being called to the Philippine Bar, as a 6th Placer, in 1993, and finishing his Bachelor of Laws from the University of the Philippines. He specializes in intellectual property and technology law as well as alternative dispute resolution (ADR), more specifically in negotiation and mediation. He is an accredited mediator of the Court of the Appeals and qualified patent agent in the Philippines. Besides practicing law and before joining public service in 2009, Mr. Ong occupied several top managerial positions, including project administrator for a major development project of the Supreme Court of the Philippines on justice reforms. He was the AssistantVice President for Corporate Affairs and Administration for Apo Cement Corporation under J.G. Summit Holdings, Incorporated, a conglomerate in the Philippines, and Vice-President for Legal and Administration in Golden ABC Incorporated, a Fashion Brand Company in Asia with a brand portfolio that includes Penshoppe, Memo, Forme, Regatta, Tyler and Oxygen. Mr. Ong also teaches in the Philippine Judicial Academy of the Supreme Court of the Philippines and taught at the College of Law and Governance of the University of San Carlos in Cebu and Ateneo Law School, LL.M. Program. He coauthored the book on trademarks entitled “Protecting Marks of Ownership.”
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
QUORUM SENSING INHIBITION BY NON-POLAR FRACTIONS FROM HALYMENIA DURVILLAEI BORY DE SAINT-VINCENT FROM BOLINAO, PANGASINAN, PHILIPPINES Dang Marviluz L. Espita1,2 and Marco Nemesio E. Montaño1* 1 The Marine Science Institute, Seaweed Chemistry Laboratories and Pilot Plant Velasquez St., National Science Complex, College of Science, University of the Philippines, Diliman 1101, Quezon City 2 Institute of Chemistry ,National Science Complex, College of Science, University of the Philippines, Diliman 1101, Quezon City Email:
[email protected] Various important biological functions including luminescence, antibiotic response, plasmid transfer, motility, virulence, biofilmformation and biofouling have been found to be regulated through the interference of bacterial communication mediated by signal molecules. Theprocess of signal interference, termed as quorum sensing inhibition (QSI), has brought about researches that screen for lead compounds that are able to block bacterial crosstalk. Bioassays involving the use of biosensors like the Chromobacteriumviolaceum have provided high-throughput means of screening for bioactive fractions from several samples. The pigment production of C. violaceum is elicited via a density-dependent cell-to-cell signaling system. The inhibition of pigment production indicates positive QSI. The availability of this method allows the screening of samples even at the field, which is advisable for samples of marine origin. Halymeniadurvillaei is a red marine algae belonging to the Order Cryptonemiales. It is commonly found in rocky substrates with frequent wave action, and is seldomlyinfestated by epiphytes and other biofouling organisms. This observation gives insight into its rich phytochemistry that may play an essential in the prevention ofits surface colonization. In this study, H. durvillaei samples were collected from Bolinao, Pangasinan in Northwestern Luzon, Philippines. Voucher specimens were deposited to the G.T. Velasquez Herbarium at the UP Marine Science Institute for proper identification and authentication. Samples for crude extraction were weighed, frozen, and lyophilized prior to extraction. Freeze-dried samples were homogenized, weighed, and macerated in methanol for 3-5 days. Following solvent extraction, bioassay-guided fractionation manifests quorum sensing inhibitory activities in DCM and hexane-drivedfractions of H. durvillaei. All experiments were done in at least triplicate. Current work is on the purification and characterization of the QSI compounds.
ISOLATION, CHARACTERIZATION, AND MOLECULAR CLONING OF A NOVEL SERINE PROTEASE-LIKE GENE OF THE ELASTASE SUB-CLASS FROM MORINGA OLEIFERA SEED EMBRYOS. Maini, ZA, Panes, VP*, Canlas, MKC, Garcia RN, Tolentino VS. Serine proteases are a wide variety of enzymes that hydrolize peptide bonds in proteins and thus have varied regulatory uses in several members of the plant genera (Antao & Malcata, 2005). In this study, RNA from varying developmental stages of Moringa oleifera were isolated and subjected to reverse transcription polymerase chain reaction (RT-PCR) and Random Amplification of cDNA Ends polymerase chain reaction (RACE-PCR) using designed gene-specific primers. Sequencing results of gel purified amplification products show a 734 and 544 base pair cDNA amplicons with a blastx (putative amino acid homolgy) score of 83% and 88% similarity to the Homo sapiens Neutrophil Elastase gene (GB ACC78413) over 155 and 140 translated amino acid residues respectively, both in the (+2) frame over three of nine different embryological developmental stages. DNA extraction to verify the gene identity of the amplicons is currently in progress, with a 1500 bp amplicon having been produced using the same gene specific primers. Sequencing and subsequent cloning of the PCR products will be perfomed. Preliminary data showing resemblance to the neutrophil elastase gene may show potential involvement in terms of elastin breakdown during seed germination, and may also indicate possible antibacterial and antiviral properties of the embryo.
SYNTHESIS, MORPHOLOGICAL, AND STRUCTURAL CHARACTERIZATION OF MOISTURE ABSORBER PARA-TOLUENE SULFONIC ACID-DOPED POLYANILINE Anelyn P. Bendoy,1 *Mylene M. Uy,1 and *Reynaldo M. Vequizo2 1 Chemistry Department, MSU-Iligan Institute of Technology 2 Physics Department, MSU-Iligan Institute of Technology A greenish black sample of polyaniline which is indicative of its emeraldine salt form was successfully synthesized through oxidative chemical polymerization using aniline monomer, ammonium peroxydisulfate as an oxidant and p-toluene sulfonic acid as acidic medium and dopant. The polymerization process was done inside an inert environment fabricated gloved box to ensure minimum initial interaction of water molecules to the samples. Dopant concentrations were varied from 0.2 M to 1.0 M at 0.2 increments. * Adviser
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Structural characterization of polyaniline samples via Fourier Transform-Infrared (FT-IR) Spectroscopy revealed a broad absorption peak at ~1115 cm-1 which is assigned C-H in-plane bending an indication that the sample is in an emeraldine salt form. In addition, it is validated by the calculated values of degree of oxidation (~40%). On the other hand, the sulfonated polyaniline was confirmed by an absorption peak at ~1034 cm-1 characteristic of SO3- group present in the structure. Hence, a site for water molecule accommodation is present which is supported by the hollow centred rod-like structure formation observed in the surface morphology of the sample using Scanning Electron Microscopy (SEM). This is substantiated by the new peaks observed at ~3239 cm-1 in the FT-IR spectra, indicative of intermolecular hydrogen bonding O-H stretch, which were obtained after exposure of sample to moisture. Furthermore, increase in weights of the sample was observed after exposure to moisture. Therefore, pTSA-doped polyaniline is a moisture absorber compound and can be used as a prototype for humidity sensing materials.
ISOLATION OF HIGH MOLECULAR WEIGHT COMMUNITY DNA FROM RUMEN DIGESTA FORMETAGENOMIC STUDIES Stephen G. Sabinay1, Antonio C. Laurena2*, Cesar C. Sevilla3, and Medino Gedeun N. Yebron, Jr.3 1 Physical Sciences Department, West Visayas State University, Iloilo City; 2Biochemistry Laboratory, 2 Institute of Plant Breeding, University of the Philippines Los Baños, Laguna; 3Animal Science Cluster, University of the Philippines Los Baños, Laguna Microorganisms in rumen digesta provide a rich source of genes for biotechnological applications. However, these microbes are difficult to grow in vitro, thus understanding their roles in rumen fermentation remains a challenge. In order to overcome the limitations of culture-based techniques, metagenomic analysis can be applied. In this study an indirect DNA extraction method was developed and evaluated by comparing it against four routinely used method designed for soil, bacteria and plant samples. The modified method facilitates the concentration of microorganims from rumen digesta and employs the use of bead-beating in the presence of high concentrations of sodium dodecyl sulfate (SDS), salt, and EDTA, with subsequent purifcation of ammonium acetate and isopropanol. This method resulted in a 2.2- to 4.4-fold increase in DNA yield when compared to four other widely used method. The community DNA recovered has an estimated maximum size of 9.4- to 23 kb and was pure enough for restriction digestion and PCR amplification making it ideal for metagenomic library construction and microbial diversity studies. In addition, the method developed in this study does not use hazardous substances such as phenol and chloroform nor does it utilize expensive spin columns for purification.
CYCLIC DIARYLHEPTANOID GARUGAMBLIN-I ISOLATED FROM THE BARK OF GARUGA FLORIBUNDA (“BOGO”) Mylene M. Uy* and Putlih Adzra T. Pautong Air-dried bark sample of Garuga floribunda (“Bogo”) was extracted with 95% ethanol and sequentially partitioned with chloroform. The concentrated chloroform extract (GFC) was fractionated employing gravity column chromatography (gcc) using gradient mixtures of hexane-ethyl acetate through ethyl acetate-methanol at 10% increment. The eluates were pooled based on their tlc profiles, resulting to a total of eight fractions which were subjected to brine shrimp lethality test, with GFC4 showing the highest toxicity (LC50=47.8 ppm). Purification of GFC4 afforded five sub-fractions (GFC4.1 to GFC4.5), among which GFC4.1 exhibited the highest toxicity (87.1% mortality) against A. salina. It was then purified, resulting to seven sub-fractions, three of which (GFC4.1.1GFC4.1.3) were sent for NMR spectroscopic analysis. Among the remaining sub-fractions, GFC4.1.5 was further purified through gcc on the bases of relative purity and weight. This in turn afforded six sub-fractions, with GFC4.1.5.2, GFC4.1.5.5, and GFC4.1.5.6 subjected to NMR spectral analyses. On the other hand, formation of crystals in GFC3, indicative of high purity, was the basis for purifying the said fraction. This was done by simply repeatedly washing the crystals with methanol since they were found to be insoluble in it. The filtrate (dark-green in color) was labeled GFC3.1. Total purification was then performed by washing the white crystals with hexane, with the filtrate labeled as GFC3.2. The isolated crystals (GFC3.3) were then submitted for NMR, MS, and X-ray Crystallographic analyses. Out of the eight sub-fractions sent, only GFC3.3 was completely characterized. Based on spectroscopic analyses, the identity of the isolate (crystals) was deduced to be garugamblin-I (C21H22O4).
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REMOVAL OF ORGANIC AND INORGANIC POLLUTANTS FROM AQUEOUS SYSTEM BY CHITOSAN MOLECULARLY IMPRINTED POLYMER IN MODIFIED AND UNMODIFIED FORM Xyza Jane Templonuevo and Soma Chakraborty, Ph.D.* Department of Chemistry, School of Science and Engineering, Ateneo de Manila University, Loyola Heights, Quezon City Water availability and the ecological and toxicological effects of both organic and inorganic pollutants render water pollution a major global concern. The target organic pollutant selected for this study is a triacylglyceride (a lipid type) which can be found in wastewater from slaughterhouses and meat processing facilities. When present in significant amount, lipids can deplete the amount of dissolved oxygen available for aquatic organisms causing disruption of the aquatic ecosystem such as loss of aquatic communities by death or migration. An economically attractive mode of water remediation is the use of chitosan, a biopolymer derived from deacetylation of the structural polysaccharide chitin. Chitin is the second most abundant natural polymer and can further be obtained by recycling discarded crustacean shell wastes from canning industries. In this study, molecular imprinting was performed on chitosan to increase its selectivity for the target pollutant. Chitosan was molecularly imprinted by crosslinking with glutaraldehyde in the presence of triolein (a triacylglyceride) as the template. Crosslinking was found to be complete after 2h reaction time at 50°C. To increase the interaction of chitosan with the template, hydrophobic modification was also done by N-acylation using octanoyl chloride. Characterization by FTIR of the molecularly imprinted and acylated chitosan are currently being performed. Template rebinding will also be investigated as a function of initial template concentration and contact time. Furthermore, since numerous studies have shown that chitosan sequesters heavy metal ion pollutants by chelation through its amino groups, the efficiency of the molecularly imprinted chitosan in simultaneous removal of an organic pollutant (triolein) and an inorganic pollutant (a heavy metal ion) in aqueous system will also be studied. FUNCTIONAL ELUCIDATION OF THREE CONTIGS OF MARSUPENAEUS JAPONICUS TO WHITE SPOT SYNDROME VIRUS (WSSV) BY dsRNA INTERFERENCE Maria Violeta Tarea, Mary Beth Maningasa,b*, Aiko Shitarac, Hidehiro Kondoc, Ikuo Hironoc a Graduate School, University of Santo Tomas, España Manila, Philippines, b Department of Biological Science, College of Science, University of Santo Tomas, España, Manila, Philippines, c Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan White spot syndrome virus remains to be the main threat in shrimp farming due to its high mortality rate of up to 100% upon infection in 3 to 7 days. To develop strategies to combat the virus, studies on different genes involved in the host-virus interactions are done to reveal their functions. In this study, contigs 17, 31 and 34 expressed in the genome of kuruma shrimp was silenced using RNA interference to elucidate its role in the shrimp immune system challenged with WSSV. Genomic DNA of the shrimp expressed all three contigs, followed by DNA sequencing. Blast analysis revealed that all three are homologous to the WSSV genome. Mortality data showed that contig 34 provided the highest protection at 25% survival at day 7 p.i. with the samples in the controls groups PBS and GFP all dead on the same sampling date. TAXONOMY AND NEW DISTRIBUTIONS OF INVASIVE SUCKERMOUTH ARMORED CATFISHES (PISCES: Pterygoplichthys) Joel M. Chavez Department of Biology, College of Science, De La Salle University The invasion of suckermouth armoured catfishes (SACs) is one of the more dramatic and traumatic events in Philippine ecology in recent times. The effects of this invasion have rippled through socio-economic dimensions as reported in local media that it has left an imprint in the consciousness of many Filipinos on the ill-effects of a lowly aquarium fish fondly called as “janitor fish.” In this paper, the complications of the mis-identities of the fish are discussed. Discussed as well are the new areas of distribution for SACs and assessments of their invasion threat levels in these areas. IDENTIFICATION OF MANGROVES, SEAGRASSES AND SEAWEEDS OF NOGAS ISLAND, ANINI-Y, ANTIQUE Laureen Morillo-Manalo and Armi May Torrechilia-Guzman* Institute of Marine Fisheries and Oceanography, College of Fisheries and Ocean Sciences, University of the Philippines Visayas Miagao, Iloilo A field survey was conducted in Nogas Island Fish Sanctuary, one of the hundreds of marine protected areas in the Philippines to identify the species of mangroves, seagrasses and seaweeds present in the area. This survey was conducted on July 2012 and participated by UP Visayas Fisheries students. Samples of mangroves were collected from the seaward to landward area while the seagrasses and seaweeds were collected on seagrass beds stretching along the front side of the island.
* Adviser
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Samples were packed in plastic bags and brought to the Fish Biology lab of the University of the Philippines VisayasCollege of Fisheries and Ocean Sciences for identification using field guides and identification keys. Eight mangrove species were found such as Avicennia marina, Bruguiera cylindrica, Ceriops tagal, Osbornia octodonta, Rhizophora apiculata, Rhizophora mucronata, Rhizophora stylosa and Sonneratia alba. Five species of seagrasses were also identified which include Cymodocea rotundata, Halodule pinifolia, Halophila ovalis, Syringodium isoetifolium and Thalassia hemprichii while there were seven species of seaweeds such as Enteromorpha sp., Gracilaria sp., Halimeda sp., Padina sp., Sargassum sp. and Turbinaria sp. The number of species found in the mangrove and seagrass beds implies that the island has a diverse and rich habitat which is beneficial for aquatic organisms like fish, molluscs and crustaceans as these areas are considered as spawning and nursery grounds where these organisms can seek refuge. DIVERSITY OF SOME ENDEMIC Nepenthes SPECIES OF THE PHILIPPINES BASED ON MORPHOLOGY, rbcL, matK, AND 18S rDNA SEQUENCES Rachel Y. Acil1, Ma. Genaleen Q. Diaz2*, Victor B. Amoroso3*, Rita P. Laude2*, Merlyn S. Mendioro2*, and Antonio C. Laurena4* 1 Graduate School, University of the Philippines, Los Baños, Laguna, 4031; 2 Genetics and Molecular Biology Division, Institute of Biological Sciences, CAS, UP Los Baños, 4031, Laguna; 3 Department of Biology, Central Mindanao University, Musuan, 8710 Bukidnon; 4 Institute of Plant Breeding, Crop Science Cluster, College of Agriculture, UP Los Baños, 4031, Laguna The form of resemblance using morphological characters were determined using numerical phenetic analysis; and the rbcL, matK, and 18s rDNA genes from the thirteen Philippine endemic Nepenthes species were partially sequenced to determine the extent of diversity of the thirteen endemic Nepenthes species. Numerical phenetic analysis partitioned the thirteen endemic species into seven distinct groupings. Molecular analysis and DNA barcoding was done using three genes: ribulose-1,5biphosphate carboxylase large sub-unit gene (rbcL), maturase-K (matK), and 18s ribosomal DNA, which were amplified and sequenced. The samples were all correctly identified up to genus level based on the three genes using nucleotide BLAST. Genetic distances were computed using Kimura 2-Parameter model and Jukes-Cantor method. The 18s rDNA gene sequences had the highest genetic divergence, followed by matK and rbcL. The phylogenetic tree based on the concatenated rbcL and matK sequences formed distinctive clades of the three individuals for each Nepenthes species. Thus, there is morphological and genetic variation among the thirteen endemic Nepenthes species. This study further proved that the two recommended barcodes, rbcL and matK, are not powerful enough to discriminate the thirteen Nepenthes species. IMMUNOMODULATORY AND CHEMOPREVENTIVE PROPERTIES OF SULFATED POLYSACCHARIDES FROM SARGASSUM SILIQUOSUM J. G. Agardh Ross D. Vasquez1,4, John Donnie A. Ramos 2,3,4 1 Department of Pharmacy, Faculty of Pharmacy 2 Department of Biology, College of Science 3 Research Center for the Natural and Applied Sciences 4 The Graduate School, University of Santo Tomas, España, Manila Sulfated polysaccharides from brown seaweeds attracted extensive interest due to their numerous biological activities and structural diversity. Sulfated polysaccharides isolated from Sargassum siliquosum J. G. Agardh were investigated for their immunomodulatory and chemopreventive potentials using peripheral blood mononuclear cells (PBMC), hepatocarcinoma cells (HepG2), renal carcinoma cells (RCC), colon carcinoma cells (HCT) and promyelocytic leukemic cells (PML) and analyzed using HPLC, Ashing-Acid Digestion Ion Chromatography and FT- IR. Chemical analysis showed that polysaccharides contain fucose (.35 mM) and mannose (37.762 mM) for AQ; fucose (.363 mM) and mannose (37.762 mM) for F1 and mannose (1.69 mM) for A1; with IR spectra comparable to standard alginate and fucoidan with sulfate with peaks at 1450-1350 cm-1, O-H stretch with strong signals between 3400-3200 cm-1, C-H stretch at 3000-2850 cm-1 ; C-O at 1300-1000 cm-1, C=O at around 1630-1695 cm-1; degree of sulfation were 11% (AQ), 3.5% (A1) and .21% (A1). AQ, F1 and A1 significantly induced proliferation of PBMCs and displayed significant antiproliferative activity in both HepG2 and renal carcinoma cells in vitro in dose-dependent manner and cytostatic effect in human colon cancer cells (p 2.5 mg/mL) radical scavenging assay (p