Figure S1. Comparison of MALDI-TOF mass spectra obtained from phage 3A purified by 500 kDa Pellicon XL 50 ultrafiltration cassette before and after 10 min ...
Intens. [a.u.]
x104 1.50
3A, ultrafiltration 2783
1.25
1.00
2999 3641 4216
0.75
6420 8095
0.50
9639 10486
0.25
13609
15852
Intens. [a.u.]
23328 25254
30383 32335
0.00
5000
3A, ultrafiltration, bME treated 2814 3003
4000
3223 3663
3000
6431
2000
7390 9635
1000
0
11320
5000
10000
13219
15859 15000
23326 20000
30381 25000
30000
35000
40000
Figure S1. Comparison of MALDI‐TOF mass spectra obtained from phage 3A purified by 500 kDa Pellicon XL 50 ultrafiltration cassette before and after 10 min treatment with β‐mercaptoethanol (bME) in 1:10 v/v ratio.
45000
m/z
Intens. [a.u.]
x104
7818
1×109 PFU/mL
1.5 23062
1.0 0.5
3904
6106
Intens. [a.u.]
0.0 8000
9550
11528
15792 14474
19104 32211
34895
38860
7817
42179
46141
48441
1×108 PFU/mL
6000 4000 2000
3901
6105
10207
23067 15797 17703
46129
Intens. [a.u.]
0
1×107 PFU/mL
1500 7812
1000
Intens. [a.u.]
500 0
1×106 PFU/mL
600
400
200
0 5000
10000
15000
20000
25000
30000
35000
40000
45000
Figure S2. MALDI‐TOF mass spectra obtained from series of 10‐fold dilutions of CsCl purified Kayvirus K1/420 with an initial titer 1×109 PFU/mL.
