phagocytosis assays in which target cells were labelled with PKH67. Percentage phagocytosis was determined by the frequency of dual expressing ...
Cancer-Oncolytic Viruses II phagocytosis assays in which target cells were labelled with PKH67. Percentage phagocytosis was determined by the frequency of dual expressing CD15+/PKH67+ cells, relative to total CD15+ cells. We established that the‘high’CD20NALM6 cells generated a significantly greater neutrophil-mediated phagocytic response to rituximab alone than the ‘low’CD20NALM6 cells with a maximum of 57% at E:T ratio of 1:5. Next, we investigated neutrophil-mediated phagocytosis following MV infection. Complement was not added to the medium. At 24 hours post infection with MVNSe, there was only a background level of phagocytosis (
