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a) b) c - PLOS
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a) b) c - PLOS
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A verage Number of. Parameters After Refinement igf1 ras pi3k il1a traf6 il6 stat3 lps tgfa tnfa mkk4
map3k7
jnk12 mek12 map3k1 akt p70s6 p90rsk msk12 irs1s.
Average MSE After Refinement
0.18
70 65
0.16
60
0.14
55
0.12
50
0.1
45 40
0.08
35
0.06
30
0.04
25
0.02 -4 10
b)
tnfa
-3
-2
10 10 10 Selection Threshold
il1a
lps
map3k7
map3k1
mkk4 jnk12
20 0 10
-1
tgfa
igf1
ras
pi3k
traf6 akt
il6
mek12
ikk p38 msk12 p53
c) NO-CYTO
TNFa
ikb
gsk3
p90rsk
histh3
creb
Ligand Stimulation IL1a
IL6
NO-INHIB MEK12i p38i PI3Ki IKKi mTORi GSK3i JNK12i
hsp27
NO-INHIB MEK12i p38i PI3Ki IKKi mTORi GSK3i JNK12i
cjun
Average Number of Parameters After Refinement
a)
IGF1
mtor irs1s
TGFa
p70s6
LPS
AKT
Ikb JNK12 p38 p70S6 p90RSK STAT3 cJUN CREB HistH3 HSP27 IRS1s MEK12
Inhibitor Measured value not reliable
0
0.25
0.5
0.75
Absolute Error
1
NO-INHIB MEK12i p38i PI3Ki IKKi mTORi GSK3i JNK12i
NO-INHIB MEK12i p38i PI3Ki IKKi mTORi GSK3i JNK12i
NO-INHIB MEK12i p38i PI3Ki IKKi mTORi GSK3i JNK12i
NO-INHIB MEK12i p38i PI3Ki IKKi mTORi GSK3i JNK12i
p53 NO-INHIB MEK12i p38i PI3Ki IKKi mTORi GSK3i JNK12i
Species’ phosphorylation
GSK3
stat3
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