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Asian Pac J Trop Biomed 2013; 3(9): 682-691
Contents lists available at ScienceDirect
Asian Pacific Journal of Tropical Biomedicine journal homepage: www.elsevier.com/locate/apjtb
Document heading
doi: 10.1016/S2221-1691(13)60139-2
襃 2013
by the Asian Pacific Journal of Tropical Biomedicine. All rights reserved.
comparative study of the antioxidant, antimicrobial, cytotoxic and thrombolytic potential of the fruits and leaves of Spondias dulcis A
Shawkat Md. Aminul Islam, Kh Tanvir Ahmed*, Mohammad Kawsar Manik, Md. Arif Wahid, Chowdhury Shafayat Ibne Kamal Department of Pharmacy, East West University, Aftabnagar, Dhaka-1212, Bangladesh
PEER REVIEW
ABSTRACT
Peer reviewer Z obaer A l M ahmud, D epartment of Clinical Pharmacy and pharmacology, F aculty of P harmacy, U niversity of Dhaka, Dhaka-1000, Bangladesh. Tel: +8801722597925 Fax: 880-2-8615583 E-mail:
[email protected]
Objective: To investigate the antioxidant, antimicrobial, cytotoxic and thrombolytic property of the fruits and leaves of Spondias dulcis (S. dulcis). Methods: Methanolic extracts of fruits and leaves of S. dulcis were partitioned with chloroform and dichloromethane. The antioxidant potential of the crude extract and partitioned fractions were evaluated in terms of total phenolic content, total flavonoid content, DPPH radical scavenging potential, reducing potential and total antioxidant capacity by specific standard procedures. The antimicrobial activity was evaluated using disc diffusion method. The cytotoxicity was evaluated by using brine shrimp lethality bioassay and compared with vincristine sulfate. The thrombolytic activity was compared with streptokinase. Results: The methanolic fruit extract exhibited the highest phenolic content, flavonoid content and antioxidant capacity, among the other extracts, with the highest DPPH radical scavenging activity at a concentration of 10 µg/mL (IC50: 1.91 µg/mL) and maximum reducing power at a concentration of 100 µg/mL (EC50: 3.58 µg/mL). Though all extract showed moderate antimicrobial activity against the bacterial strains, weak or no activity against fungus. The range of LC50 value of all extracts was 1.335-14.057 µg/mL which was far lower than the cut off index for cytotoxicity. All extracts exhibited statistically significant (P
