13. Table S3. Primer sequences used in ChIP experiments. Primer sequences used in ChIP experiments for Src PPREs. Forward (5' -> 3'). Reverse (5' -> 3').
Page 1. Table 1: Primer-Oligonucleotide Sequences. Name. Primer Sequence 5'-3'. Fragment length (including Primers). H2Dt1F. GGATGCTATGACTCACCA.
siRNA Target Gene. siRNA sequence (5' to 3'). Human c-Kit. 1. UCAUUCUUGAUGUCUCUGGtt. 2. UUUGAGUUCAGACAUGAGGtt. 3.
Table S3. Primer sequences for qPCR. Gene. Forward primer. Reverse primer. TNN1. GGGTGACTGGAGGAAGAACG. AGCAAGCTGGCCTCTATTGT. CTST.
Supplemental Table 1. Sequences of specific primer pairs. Primers. 5'-3'. CDK2 (human). CATGGATGCCTCTGCTCTCAC. GACCCGATGAGAATGGCCAGAA.
Rat qPCR Primer Sequences. MMP2. F: GCTGATACTGACACTGGTACTG. R: CACTGTCCGCCAAATAAACC. MMP9. F: CTTGAAGTCTCAGAAGGTGGATC.
F. TAAGGCTCCAGTCACCTGCT. 255. R TTCCAGCTTTGGCTGTCTCT. Ccl12 mouse. F. GGGAAGCTGTGATCTTCAGG. 177. R GGGAACTTCAGGGGGAAATA.
Hg mix hg19. (chry). 16,638,804. 7,879,415 hg38. (chry). 14,526,924. 8,011,374 chang e. A to G. A to C. 12. 5.36. M438, P215. S238, L460. 12 approx. hg: 1- ...
DETECTION OF TRYPANOSOMA BRUCEI GAMBIENSE ... in diagnosis, treatment, and epidemiology of human African trypanosomiasis (sleeping sickness).
entry site found in the SIV leader sequence but not in HIV-1. (26, 32). ... generate mutant constructs (primers pSD and pSgag1 were used for SD deletion,.
To ensure higher accuracy and stringency of the regions amplified, two rounds of ‘denaturation-annealing-extension’ (step 2a-c, and 3a-c) were done.
A.,B
Annealing
temperature varied depending on the assay and they are summarized in (c).C Number of cycles varied as well depending on the assay and they are summarized in (c). (c) Assay
Number of cycles for step 3a-c in (b)
Line-1
Annealing Temperature Step 2b
Step 3b
30
58
61
Alu
40
58
60
H19 promoter DMR
40
60
60
IGF2/H19 ICR
40
56
60
Supplementary Table 2. Genomic coordinates for CpG unit analysed for IGF2/H19 ICR, H19 promoter DMR, Alu and LINE-1. CpG unit IGF2/H19 ICR IGF2/H19 ICR CpG1
H19 promoter DMR CpG17-18 H19 promoter DMR CpG20 Alu Alu CpG6 CpG6 n/aa Alu CpG7 CpG7 n/aa Alu CpG16 CpG16 n/aa LINE-1 LINE-1 CpG1 CpG1 n/aa LINE-1 CpG2 CpG2 n/aa LINE-1 CpG3 CpG3 n/aa a n/a indicates the genomic coordinates for Alu and LINE-1 are not available (n/a) as they are repeat elements.
Analysed as a discontinuous variable, with ‘yes’ for more than 400mg/day of folic acid supplement intake at least 1 month during the 3 months before pregnancy. Periconceptional Analysed as a discontinuous variable, with ‘yes’ for more folate supplements than 400mg/day folic acid supplement intake at least 4 weeks during the first 12 gestational weeks Serum vitamin B12 Analysed as a continuous variable, measuring maternal serum vitamin B12 at 28 weeks of gestation (third trimester). Serum folate Analysed as a continuous variable, measuring maternal serum folate at 28 weeks of gestation (third trimester). Periconceptional Analysed as a discontinuous variable, with ‘yes’ for any alcohol quantity of alcohol consumption on a weekly basis before knowing they were pregnant Periconceptional Analysed as a discontinuous variable, with ‘yes’ for any smoking quantity of cigarettes on a weekly basis before knowing they were pregnant. Gestational diabetes Analysed as a discontinuous variable, with ‘yes’ for presence of gestational diabetes Maternal Analysed as a continuous variable. High correlations within macronutrients measures of carbohydrate, protein and energy intake (data obtained via validated food frequency questionnaire) were handled by using a principle components analysis to derive summary variables, whereby the first principle component obtained was used as the ‘macronutrient’ variable in the analysis. Maternal stress Analysed as a continuous variable, measuring maternal stress at third trimester. Ratio of serum Analysed as a continuous variable whereby the ratio was vitamin B12/serum calculated by dividing maternal serum vitamin B12 values folate with maternal serum folate values 28 weeks of gestation IVF Analysed as discontinuous variable, with ‘yes’ for twins conceived through IVF or ICSI, and ‘no’ for twins conceived without medical help. Placenta weight Analysed as a continuous variable. Total placental weight value was divided equally between co-twins when values for separate placental weight values were unavailable. Umbilical cord Analysed as a discontinuous variable, with cord inserted insertion within the 50% radius of the placenta were considered ‘Central’ cord insertion. Data for all continuous variables were converted to z-scores by subtracting the mean of the variables from the variable value, and divide by the standard deviation.
Supplementary Table 4. Univariate regression analysis of absolute within-pair methylation discordance associated with all IVF twins Region Coefficient P-value 95% Confident Interval LINE -0.4%* 0.016 - 0.7% - 0.1% Alu 0.2% 0.656 -0.7% 1.1% IGF2/H19 ICR -0.3% 0.636 -1.7% 1.0% H19 promoter DMR 0.7% 0.421 -1.1% 2.6%
Supplementary Table 5. Inter-individual variation in DNA methylation in Alu, LINE-1, IGF2/H19 ICR, and H19 promoter DMR Assay Alu LINE-1 IGF2/H19 ICR H19 promoter DMR
