A Randomized Study Comparing Methylprednisolone Plus ...

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Plus. Chlorambucil. Versus. Methylprednisolone. Plus. Cyclophosphamide ... 6 mo: 3 mo with corticosteroids and. 3 mo with one cytotoxic drug. Among.
A Randomized Study Comparing Methylprednisolone Plus Chlorambucil Versus Methylprednisolone Plus Cyclophosphamide in Idiopathic Membranous Nephropathy CLAUDIO PONTICELLI,* PAOLO ALTIERI,t FRANCESCO SCOLARI, PATRIZIA PASSERINI,* DARIO ROCCATELLO, BRUNO CESANA,” PATRIZIA MELIS,t BRUNELLA VALZORIO, MAURO SASDELLI,1 SONIA PASQUALI,# CLAUDIO POZZI,** GIUSEPPE PICCOLI, ANTONIO SIRO SEGAGNI, FRANCESCO ANTONUCCI,5 MAURO DUGO,111’ MARILENA MINARI,11 ALFIO SCALIA, LUCIANO PEDRINI,*** GABRIELE PISANO,ttt CLAUDIO GRASSI,t MARCO FARINA, and ROBERTO BELLAZZI’1111 *Dj,jsjone

di Nefrologia

Regionale

Spedali

Ospedale

Maggiore

Lecco;

“Ospedale

Provinciale

Feltre;

Zonaie

Uboldo

Magenta;

mide

To may

assess

have

whether

idiopathic merubranous mens based on a 6-mo methylpnednisobone with cycbophosphamide. nous

ncphropathy

domized

and

to be given

3 consecutive mg/kg

per

Civile

1Ospedale

chborarubucil

or index

chborambucib Patients with

by

cycbophospha-

in patients

with

or mcthylprcdnisobone biopsy-proven membrasyndrome

were

ran-

(1 g intravenously

oral

ructhylpnednisobone,

27 d) alternated

d for

(0.2

every other month either per d for 30 d) or cycbophosphamide

rug/kg

with

Among

87 patients followed for at beast 1 yr. 36 of 44 (82%; confidence interval [CI], 67.3 to 91 .8%) assigned to

methylprcdnisobone tial

remission 95%

There

is now

CI, 80.9

methylprednisobonc

3 mo

and chborambucib of

(93%;

whole

the to

controlled and

ncphrotic

98.5%)

treatment with

lasted

one

entered syndrome,

Parma;

##Ospedale

Provinciale

Lodi;

and

hiOspedale

chbonambucil

complete versus

on par40

group

(30.5%)

group

(25%)

had

of

43

and a relapse

(P

remained

0.01)

and

values

in the

in the

phamide

ncphrotic both

and

treatments

developed may

renal

9: 444-450,

two

cancer.

in the

is

but

at least

(1-3)

and

3 yr.

of side

none

in the

zoster.

concluded

One that

in

nephropathy

in favoring

for

Six

cycbophos-

herpes

It

with 3 yr.

because

merubranous

effective

function

2 and

group from

idiopathic

be

attained chloram-

compared for

the treatment

suffered

with

when

chlorambucil

group group

who

cycbophosphamidc syndrome between

followed

group

in the

patients

preserving

groups

did not complete

cycbophosphamide per

unchanged

cohort

patients

patients

1 1 of 36 in the

of plasma creatininc improved in for I yr for both treatment groups

chborambucib

group Four

Of

10 of 40 in the of the nephrotic

reciprocal followed




with

corticosteroids,

a positive

serum

with

albumin

con-

The

per

a

syndrome

for the study.

creatinine

or cycbosporine;

of

and

3.5

were

a plasma

previous

drugs,

bodies antigen

between

gIL),

criteria

deciliter

(150

irumunosupprestest

for anti-DNA

anti-

(and antinuclear antibodies in many patients) or hepatitis B or hepatitis C virus antibodies; a positive Veneral Disease

Research plement

Laboratory component

test: or low serum C3 or C4. A clinical

litus. malignancy. sure to drugs that

chborambucil but

at a dose

count

decreased

dose

wt per d given cycle

concentrations of the diagnosis of diabetes

systemic lupus erythematosus, could induce membranous

infections, nephropathy

was

mg/kg

group

mide

the was

mg/kg

5000

cornmel-

or expowere also

Steroids

period.

The

were use

discouraged

but

Clinical

excretion

a remission.

at

I wk.

a normal

plasma

syndrome

was

3.5 g/d

worsening

was

Gbomerular

stages

reich

ci’

with

tubular

were

atrophy

biopsies

were

were

was an open-label by the institutional patient

ruent

regimens,

sample

size

probability

gave were

informed

written

assigned

according a decrease

to of about

ruethylprednisobone

25%

with

year,

and

plus

every

side were

cytotoxic

effects

of therapy.

measured

every

drug,

and

In our previous criteria,

only

start

early,

to prevent

24-h was

syndrome

without complications

a run-in

period,

was from

of

treat-

order.

thereafter. urinary questioned

At each protein about

of efficacy

and

administration

rates and platelet of the

visit.

patients

had a spontaneous

6 mo (4). Therefore, a potentially

of the nephrotic

with

syndrome.

the same

reciprocal

beneficial Patients

treatment assigned

to

remission 0.2 1 and

2

concentration. as an

increase

in

who

had

in plasma

the baseline

value.

remission,

complete

was estimated according to of the hazard functions

performed

on

or partial

of the plasma

creatinine

treatments were compared measurements (10), with

patient”

the

cumulative

remission.

A further

of the remission

factor

at two

levels

(every

and

and

time

6 mo).

proteinuria

cedure icance

for correction of the degrees of freedom of the within-patient terms was followed. been

carried

trend (second degree). creasing-increasing-decreasing

out

The

as a fixed,

at 2 1 levels

has

by

two

values

for

“within-patient”

Geisser-Greenhouse

means

( I I ) pro-

for statistical In addition,

of orthogonal

of a linear and then

signifa trend

polynomial

increasing/decreasing decreasing (or

higher than the have therapeutic

third). Indeed, impact.

trend versa)

vice

of an increasing-decreasing-increasing trend (third degree) of a mean

ating pattern (degree overall pattern could

time

The test.

by mixed-factorial ANOVA for treatment as a fixed, “between-

factor

or defluctu-

the shape

of an

Results Among tion,

97 patients

two

were

underlying after

remission

we preferred

partial

(partial or complete) on the total follow-up for each patient. treatments were then compared by the Wilcoxon rank sum

of

excretion symptoms

remission.

as an increase

as the percentage

a baseline

plasma

been

was obtained

contrasts to assess the presence (first degree), of an increasing

at of

was

permitted.

fulfilled as suggested by the log have been shown for illustrative

complete

analysis

visit,

selected

have

with

A

erythrocyte,

patients

tests

of patients

to

of about

and

complete/partial

be considered survival curves

necessary

(2) at power

10 d during

with

within

random

of response

at each

experience

was apcenter,

of the two

per group

Leukocyte,

one of 39 untreated

of the nephrotic

fob-

0.05). Patients were examined 6 ruo. every 2 mo until the end

7 to

repeated

to one

chborambucil =

3 to 6 ruo

creatinine concentrations and were measured, and the patient counts

No

At the coordinating

a center-stratified 50 patients

with a two-tailed test (alpha every month during the first

possible

fibrosis

or absent.

consent.

consecutively

80%

first

index

cannot plots,

only;

proportion

The

0.80 least the

interstitial

as present

purposes

of Ehren-

multicenter study. The protocol review board of the coordinating

of approximately

demonstrate

and

biopsy

microscopy.

to the system

sclerosis

classified

renal

study

Analyses

Cumulative

the two repeated

Design

patients

according

each electron

performed.

This proved center,

examined and

glornerular

Study

each

center

classified

al. (8). Mesangial

low-up

and

study

immunofluorescence.

the

I wk in patients

over

proportionality (-log) survival

at each

were

defined

50%

Histology light.

of

creatinine

defined

of at least

Renal

by

less of

inhibitors

to between

for at least

remission alone, and thereafter of relapses Kaplan and Meier (9). Because assumption

pathologist

end

drugs

excretion

for exclusion.

The

the

discon-

3 ruo with the same of the two cytotoxic the

Hypolipemic

criteria

specimen

it was

enzyme

of protein

with

concentration

Statistical

stopped

g/d for at least

than

Stable

and

or partial

nephrotic

creatinine

by 50%.

leukocyte b0’1L),

X

in the rate of urinary

Relapse

the

(5

as a complete

1 wk,

to more

cycbophospha-

as a reduction

g/d for at least of

by oral

to the

of methyl-

defined

in the rate

proteinuria

cycles

was defined to 0.2

as a reduction

three

6 mo for both groups; and 3 mo with either

was

of treatment

assigned

cycle if the leukocyte count was (3 X b0#{176}IL).The total duration

not prohibited.

remission

protein

Patients

angiotensin-converting

response

Complete

with days

27 d, in a single 1 mo

millimeter

reduced

completely

of

for by

same

cubic

was

treatment

wt per d. If a patient’s

per

agent

orally

replaced

body

of

445

on 3 consecutive

followed

received

below

cycles

per d, orally).

chlorambucil

of 2.5

of the cytotoxic

drugs.

three

each (0.2

Nephropathy

intravenously

body

and

treatment, therefore, was doses of methylprednisobone

exceeding

of