ABC - PLOS

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NeuroD1. AGGCCCCAGGGTTATGAGAC TGAGTCCTCCTCTGCGTTCA. 218. Nkx2.2. GCACCGAGGGCCTTCAGTA. CCGGGGTCTCCTTGTCATT. 123. Pax4.
A. Template

Forward (5’-3’)

Reverse (5’-3’)

Gapdh GFP HA-Ngn3 hsNgn3 NeuroD1 Nkx2.2 Pax4 Pcp4 Pcsk1 Rab26 Scg3 Scgn Sim1

ACCACAGTCCATGCCATCAC GTCCAGGAGCGCACCATCT GCTACCCATACGATGTTCCA CCACGGCCCTCGCTGCTC AGGCCCCAGGGTTATGAGAC GCACCGAGGGCCTTCAGTA CGGCTAGCAGTCAGTGGAAT CAACCAATGGAAAAGACA GGGTGGAGAAGATGGTGGAT CAGGAAGAAGACCCCCAAGA CAACCCAGGAGGAAAGACAG CAGAAGTGGATGGGTTTGTC AGAAGCCTATGAAAACAGCA

TCCACCACCCTGTTGCTGTA GTTCTTCTGCTTGTCGGCCA CAGTCACCCACTTCTGCTTC GCGGACGTGGGGCAGGTC TGAGTCCTCCTCTGCGTTCA CCGGGGTCTCCTTGTCATT GCAGGACTCGGTTGATGGA CCAGCCTTCTTCTTCTG CGCAGGGTAAGGAAGAAGCA TTTGTTCCGGAAGTCAATGC CCTCGGCTTCTTCCTTGT GTTGATTTTCAGCCCAAGAC CATTAATGGAAGCCAGTTG

cDNA PCR product (bp) 452 200 193 155 218 123 292 151 460 307 203 167 272

B. Template Dll1 Gcg Gck Hes1 Ins Insm1 MafA NeuroD1 Nkx2.2 Pax4 Ppia Syp

Assay ID Hs00194509_m1 Hs00174967_m1 Hs00277220_m1 Hs00172878_m1 Hs00355773_m1 Hs00357871_s1 Hs01651425_s1 Hs00159598_m1 Hs00159616_m1 Hs00173014_m1 Hs99999904_m1 Hs00300531_m1

C. Template

Forward (5’-3’)

Reverse (5’-3’)

Ngn3 NeuroD1 BRCA-1 CTLA-4

TGGCCCGGGCGAAGCAGATA CCCTCTATCCCCGTCCCTTCTG GAGTCCTAGCCCTTTCACCCATAC ACAAGGCTCAGCTGAACCTGGGT

GCCCCAGCCCCAAAGGAGAAAA CCACGTGACCTGCCCATTTGTA GTGATGTTCCTGAGATGCCTTTG CTGCCGCCCAACTGCTCC

genomic PCR product (bp) 117 162 288 259