PCR products were normalized to RNU6. (c) Nfatc1, Acp5,. Mmp9, Clcn7 and Ctsk mRNA levels in whole-bone tissues of. HS and Control mice. (d,e) Serum ALP ...
d Control HS
10
5
0 *
15
3 **
e Control HS *
*
10
5
0
k
Control HS
C ts
*
lc n7
0
*
C
1
2
p9
0.0 2
4
M m
0.5
5
5
1.0 *
Relative mRNA level in whole-bone tissue
4
Ac p
1.5 3
tc 1
2.0
N fa
tr o l H S
2.5
NT XI
*
Concentration (ng/ml)
C on
* Relative miR-214 level in serum exosomes
b
CT XI
N
15
C
C on tro l H S
a
O
AL P
Concentration (ng/ml)
Relative miR-214 level in serum
Supplementary Figure 8 c
**
**
1
0
Supplementary Figure 8. Changes in circulating miR-214 levels in exosomes from hind-limb suspended mice and their inhibitory role on osteoblast activity. (a) qRT-PCR analysis of miR-214 levels in serum of HS and control mice. Control, n=3, HS, n=5. The PCR products were normalized to cel-miR-39. (b) qRT-PCR analysis of miR-214 levels in exosomes from serum of HS and control mice. The PCR products were normalized to RNU6. (c) Nfatc1, Acp5, Mmp9, Clcn7 and Ctsk mRNA levels in whole-bone tissues of HS and Control mice. (d,e) Serum ALP, OCN, CTX-I and NTX-I levels in HS and control mice were analyzed by ELISA. n=3. The data represent the mean ± SEM. *P
