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CORRELATION BETWEEN KI-67 AND TELOMERASE EXPRESSION WITH IN SITU HYBRIDIZATION FOR HIGH-RISK HUMAN PAPILLOMAVIRUS ARIANNA VEGA-PEÑA1, BERENICE ILLADES-AGUIAR1, EUGENIA FLORES-ALFARO1, ESTHER LÓPEZ-BAYGHEN2, ELBA REYES-MALDONADO3 and LUZ DEL CARMEN ALARCÓN-ROMERO1* 1
Laboratorio de Investigación en Citopatología y de Biomedicina Molecular, Unidad Académica de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México 2 Laboratorio de Genética y Biología Molecular, CINVESTAV, DF, México 3 Laboratorio de Citología, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, DF, México Abstract - The objective of this research was to evaluate the relationship of Ki-67 and telomerase expression with the progression of cervical intraepithelial neoplasia (CIN) and the physical state of the DNA of high-risk human papillomavirus (HR-HPV) types. A comparative study was done on 80 biopsies of human (female) cervical tissue, distributed in the following manner: 20 CIN-negative biopsies and 60 CIN-positive biopsies of varying grades. The detection of the proteins Ki-67 and telomerase was performed through immunohistochemistry; the detection of HR-HPV, by in situ hybridization. The expression of Ki-67 and telomerase increased with the progression of the CIN lesion (p
