depends on the photogenerated current Iph(Ï) and the open circuit voltage Voc(Ï). A PC1D [12] model determines both Iph(Ï) as well as Voc(Ï) depending on the ...
Nov 6, 2007 - However, the determination of the concentration of this metabolite ... (1H MRS) in order to perform absolute quantification of carnosine in vivo.
9 Oct 2009 - foods requires estimation of the amount of the transgenic event relative ... ratio of these absolute copy numbers determined by dPCR was found ...
thetic isotope-labeled peptides were used as internal standards, to measure the molar abundance of 32 key. PSD proteins in forebrain and cerebellum.
A need exists for technologies that permit the direct quantification of differences in ... proteomics seeks to provide information about both protein and modified ...
... is extremely selective, requires only a single dispensing step on a stan- .... Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, ...
dependent creep-recovery response induced by acoustic radiation force. The shear ..... [8] D. T. Blackstock, Fundamentals of physical acoustics: Wiley,. 2000.
(B) Real-time RT-PCR. The reverse transcription reaction is carried out as described above. All subsequent steps are carried out by the thermal cycler/detector ...
Apr 14, 2013 - (for example, PIAS4, NFIB, SMAD2, RARγ and FOSL2) were expressed within approximately the same copy-number range as. PPARγ or RXRα ...
Introduction. Absolute quantification (AQ) of MR-spectra is a useful tool for detailed studies of tis- sue metabolism in vivo in a non-invasive manner. Previously ...
... free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for .... The de novo peptide sequence is included in bold as part of SuSy2 isoform. ... Stable isotope-labelled leucine residues are marked in bold. TC code. Name.
Oct 15, 2013 - profiling and absolute quantification of proteins involved in molecular ... of chemically synthesized peptide standards known as AQUA peptides ...
Dec 3, 2015 - 1 Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis ... Institute of Biomedical Imaging and Bioengineering.
Jun 4, 2008 - Koch W, Mustafa M, Zach C, Tatsch K. Influence of movement on FP-CIT SPECT ... Cherry S, Sorenson JA, Phelps ME. Physics in nuclear ...
the PDZ domains of the PSD-95 family of scaffold proteins, which are highly ...... perhaps the best characterized scaffold protein of the PSD. Befitting its fame ...
âsomatomedinâ hypothesis, IGF-I is mainly produced in the liver under the control of GH from the anterior pituitary. After its release into the circulation, IGF-I acts ...
Sep 20, 2014 - Some studies quantify peptides using labeled peptide internal standards as a ... and deuterium water (D2O) were obtained from ISOTEC Inc.
not been investigated to date. Hence we used the heavy isotope-labeled tryptic peptides spanning the inhibitory site as internal standards and quantified all four ...
ory, RIKEN-MIT Neuroscience Research Center, Howard Hughes. Medical ...... Link, A. J., Eng, J., Schieltz, D. M., Carmack, E., Mize, G. J., Morris, D. R.,. Garvik ...
Biotechnology, Research Triangle Park, NC, 5BASF Plant Science,. Research Triangle Park, ND, 6Dow AgroSciences, Indianapolis, IN,. 7Bayer SAS, Sophia ...
Nov 8, 2014 - Practice; Waveland Press: Prospect Heights, IL, 1990. (6) Li, M.; Guha, S.; Zangmeister, R.; Tarlov, M. J.; Zachariah, M. R.. Aerosol Sci. Technol.
Methods: The SimSET Monte Carlo (MC) code was used ... For correspondence or reprints contact: Dome`nec Ros, PhD, Unitat de ... projector equation (24,25). ..... SPECT in diagnostic work-up of Parkinson's disease: a decision-analytic ap-.
Jun 15, 2003 - 3.2 Monte Carlo codes for particle transport . ... 3.4 Validation of the modified SimSET code . .... C. D
is the state of the art and is in general recommended. ..... general, the distribution of the radioactivity might not follow this assumption. Seo et al. ... should get a full-rank transfer matrix. .... SPECT/CT systems and iterative reconstruction so
Nov 17, 2010 - Isotope labeled heavy peptides were used for absolute quantification of specific .... AQUA peptides were obtained from ThermoFisher Scientific.
TECHNICAL NOTE pubs.acs.org/jpr
Absolute Quantification of Allergens from Complex Mixtures: A New Sensitive Tool for Standardization of Allergen Extracts for Specific Immunotherapy Ulla Sepp€al€a,*,†,# Claire Dauly,‡,# Sarah Robinson,§ Martin Hornshaw,§ Jørgen Nedergaard Larsen,† and Henrik Ipsen† †
Global Research, ALK-Abello, Hørsholm, Denmark Thermo Fisher Scientific, Courtaboeuf Cedex, France § Thermo Fisher Scientific, Hemel Hempstead, United Kingdom ‡
ABSTRACT: Products for specific diagnosis and immunotherapy of IgE-mediated allergies are currently based on natural extracts. Quantification of major allergen content is an important aspect of standardization as important allergens particularly impact vaccine potency. The aim of the study was to develop a mass spectrometry (MS) based assay for absolute quantification of Timothy (Phleum pratense) pollen allergens Phl p 1 and Phl p 5 in P. pratense extract. High-resolution and accurate mass (HRAM) MS was selected for its ability to detect peptides with high selectivity and mass accuracy (
