Oct 1, 2014 - Background: The epithelialmesenchymal transition (EMT) is an essential process in the tumor progression and metastasis. In human prostate ...
01/07/2016
Abstract 88: Epithelialmesenchymal transition occurs in preneoplastic and neoplastic lesions of canine prostate | Cancer Research
Tumor Biology
Abstract 88: Epithelialmesenchymal transition occurs in preneoplastic and neoplastic lesions of canine prostate Carlos Eduardo FonsecaAlves, Igor Simoes Tiagua Vicente, Luis Gabriel Rivera Calderon, Andre Augusto Justo, Silvia Regina Rogatto, Renée LauferAmorim DOI: 10.1158/15387445.AM201488 Published 1 October 2014
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Proceedings: AACR Annual Meeting 2014; April 59, 2014; San Diego, CA Abstract Background: The epithelialmesenchymal transition (EMT) is an essential process in the tumor progression and metastasis. In human prostate carcinoma (PCa), the upregulation of cytokeratin and Ecadherin and downregulation of vimentin have been associated with aggressive phenotype and poor prognosis. Due to the importance of canine cancer model it was evaluated the immunoexpression of AE1/AE3, Ecadherin and vimentin in canine prostatic lesions. Patients and Methods: A total of 75 prostatic tissues formalinfixed paraffin embedded from dogs was selected: 10 normal prostatic tissues, 20 benign prostatic hyperplasia (BPH), 25 proliferative inflammatory atrophy (PIA) and 20 PCa. AE1/AE3 was detected with a monoclonal antibody (Invitrogen, 180132) at a 1:300 dilution, applied for 45 min at room temperature (RT). The antibody against Vimentin (V9, Invitrogen) and E cadherin (NCH38, Dako cytomatiomn) were monoclonal mouse antibodies, used at a 1:300 and 1:200, respectively, for 45 min at RT. The immunolabelling was performed by a polymer method (Histofine, Nichirei Biosciences,). A negative control was performed for all antibodies by omitting the primary antibody and substituting with Trisbuffered saline. The percentage of CMYC, Ecadherin, and p63 positive cells per lesion was evaluated according to Prowatke et al. (2007). The samples were scored separately according to staining intensity and graded semiquantitatively as negative, weakly positive, moderately positive, and strongly positive. The score was done in one 400 magnification field, considering only the lesion, since this was done in a TMA core of 1 mm. For statistical analyses, the immunostaining classifications were reduced to two categories: negative and positive. The negative category included negative and weakly positive staining. Chi
http://cancerres.aacrjournals.org/content/74/19_Supplement/88
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01/07/2016
Abstract 88: Epithelialmesenchymal transition occurs in preneoplastic and neoplastic lesions of canine prostate | Cancer Research
square or Fisher exact test was used to determine the association between the categorical variables. Results: All prostatic normal and BPH tissue were positive for cytokeratin, Ecadherin and negative for vimentin. Similarly, all PIA samples were positive for AE1/AE3. From those samples, 48% (12/25) were also positive for vimentin. 55% of PCa (11/25) was positive for vimentin and among these samples 75% (6/11) was also positive for AE1/AE3 and 45% (5/11) was negative for AE1/AE3. PIA and PCa presented a higher number of vimentin positive cells when compared with normal tissue (p=0.032). Ecadherin expression had no statistical difference among diagnosis groups, but we found a higher number of positive cases, with more than 51% of positive immunostaining in BPH and PIA (81.25% and 78.60% of the cases, respectively) than in PCa (55.55%). Conclusion: The carcinogenesis process regarding prostatic epithelial cells in dogs showed higher vimentin protein expression associated with concomitant loss of the cytokeratin and Ecadherin, similar in humans. Financial Support: Sao Paulo State Foundation (FAPESP) and CNPq. Citation Format: Carlos Eduardo FonsecaAlves, Igor Simoes Tiagua Vicente, Luis Gabriel Rivera Calderon, Andre Augusto Justo, Silvia Regina Rogatto, Renée LauferAmorim. Epithelialmesenchymal transition occurs in preneoplastic and neoplastic lesions of canine prostate. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 59; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 88. doi:10.1158/15387445.AM201488 ©2014 American Association for Cancer Research.
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