Activity and expression of Na /H exchanger isoforms in ... - Springer Link
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Activity and expression of Na /H exchanger isoforms in ... - Springer Link
Mar 17, 2005 - activity in the term placental syncytiotrophoblast is ab- sent from BM. NHE activity in the MVM is attributable predominantly to NHE1. Keywords ...
P. F. Speake Æ K. J. Mynett Æ J. D. Glazier S. L. Greenwood Æ C. P. Sibley
Activity and expression of Na+/H+ exchanger isoforms in the syncytiotrophoblast of the human placenta
Received: 22 December 2004 / Accepted: 3 January 2005 / Published online: 17 March 2005 Springer-Verlag 2005
Abstract The purpose of this study was to compare Na+/H+ exchanger (NHE) activity in the microvillous (MVM) and basal (BM) plasma membrane of the human placental syncytiotrophoblast and to determine the relative contribution of various NHE isoforms to this activity. Uptake of 22Na into isolated MVM vesicles in the presence of a H+ gradient, at initial rate, was fourto fivefold higher than that by BM vesicles (214±28 vs. 49±9 pmol/mg protein per 30 s, respectively, means±SEM, n=8, 6, P