Activity and expression of Na /H exchanger isoforms in ... - Springer Link

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Mar 17, 2005 - activity in the term placental syncytiotrophoblast is ab- sent from BM. NHE activity in the MVM is attributable predominantly to NHE1. Keywords ...
Pflugers Arch – Eur J Physiol (2005) 450: 123–130 DOI 10.1007/s00424-005-1382-x

I O N CH A N N E L S , T R A NS P O R T ER S

P. F. Speake Æ K. J. Mynett Æ J. D. Glazier S. L. Greenwood Æ C. P. Sibley

Activity and expression of Na+/H+ exchanger isoforms in the syncytiotrophoblast of the human placenta

Received: 22 December 2004 / Accepted: 3 January 2005 / Published online: 17 March 2005  Springer-Verlag 2005

Abstract The purpose of this study was to compare Na+/H+ exchanger (NHE) activity in the microvillous (MVM) and basal (BM) plasma membrane of the human placental syncytiotrophoblast and to determine the relative contribution of various NHE isoforms to this activity. Uptake of 22Na into isolated MVM vesicles in the presence of a H+ gradient, at initial rate, was fourto fivefold higher than that by BM vesicles (214±28 vs. 49±9 pmol/mg protein per 30 s, respectively, means±SEM, n=8, 6, P

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