Additional file 2 (page 1) Data validation: microarray ...

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Data validation: microarray data vs. real time RT-PCR data of adipo-related genes during adipogenesis for timepoints 5 (day+2), 7 (day+10), 8 (day+15). -6. -4.
Additional file 2 (page 1) D_tp

µArray_HAD_tp5

Data validation: microarray data vs. real time RT-PCR data of adipo-related genes during adipogenesis for timepoints 5 (day+2), 7 (day+10), 8 (day+15)

µArray_HAD_tp7 µArray_HAD_tp8 RT-PCR_HAD_tp5

Data validation: microarray data vs. real time RT-PCR data of adipo-related genes during osteogenesis for timepoints 5 (day+2), 7 (day+15), 8 (day+24)

-4

-2

log2 ratio Ref-TP

RT-PCR_HAD_tp8

2

4

6

8

-6

10

CEBPa

CEBPa

CEBPb

CEBPb

CEBPd

CEBPd

PPARa

PPARa

PPARd

PPARd

PPARg

PPARg

CREB

CREB

FXR

FXR

SREBP

SREBP

GPDH

GPDH

FABP4

FABP4

FABP5

FABP5

COL6A2

COL6A2

GLUT4

GLUT4

GCCR

GCCR

IP-R

IP-R

ADRB1

ADRB1

ADRB2

ADRB2

ADRB3

ADRB3

UCP2

Gene

Gene

-6

µArray_HOD_tp7 µArray_HOD_tp8 RT-PCR_HOD_tp5 RT-PCR_HOD_tp7

RT-PCR_HAD_tp7

log2 ratio Ref-TP 0

µArray_HOD_tp5

UCP2

UCP3

UCP3

AGT

AGT

LEP

LEP

PTX3

PTX3

DIPA

DIPA

PAI

PAI

IL6

IL6

PLIN

PLIN

TNFa

TNFa

Adiponectin

Adiponectin

Adipsin

Adipsin

LPL

LPL

FASN

FASN

HSL

HSL

PEPCK

PEPCK

GATA2

GATA2

GATA3

GATA3

Id1

Id1

Id2

Id2

Id3

Id3

FOXO1

FOXO1

-4

-2

0

RT-PCR_HOD_tp8

2

4

6

8

10

Additional file 2 (page 2) µArray_HAD_tp5

Data validation: microarray data vs. real time RT-PCR data of osteo-related genes during adipogenesis for timepoints 5 (day+2), 7 (day+10), 8 (day+15)

µArray_HAD_tp7 µArray_HAD_tp8 RT-PCR_HAD_tp5

Data validation: microarray data vs. real time RT-PCR data of osteo-related genes during osteogenesis for timepoints 5 (day+2), 7 (day+15), 8 (day+24)

-6

-4

-2

0

log2 ratio Ref-TP

RT-PCR_HAD_tp8

2

4

6

8

-6

10 CBFA1

CBFA1 Osteonectin

Osteonectin

Osteopontin

Osteopontin

MSX2

MSX2

COL1A1

COL1A1

COL1A2

COL1A2

Osteoprotegerin

Osteoprotegerin

BMP2

BMP2

BMP4

BMP4 BMP6

Gene

Gene

BMP6 BMPR1A

µArray_HOD_tp7 µArray_HOD_tp8 RT-PCR_HOD_tp5 RT-PCR_HOD_tp7

RT-PCR_HAD_tp7

log2 ratio Ref-TP

µArray_HOD_tp5

BMPR1A

BMPR1B

BMPR1B

BMPR2

BMPR2

ERa

ERa

VDR

VDR

MATN1

MATN1

MATN3

MATN3

NOG

NOG

FOSL1

FOSL1

FOS

FOS

FOSB

FOSB

MGP

MGP

-4

-2

0

RT-PCR_HOD_tp8

2

4

6

8

10

Additional file 2 (page 3) Data validation: microarray data vs. real time RT-PCR data of potential self-renewal-associated genes during adipogenesis for timepoints 5 (day+2), 7 (day+10), 8 (day+15)

µArray_HAD_tp5 µArray_HAD_tp7 µArray_HAD_tp8 RT-PCR_HAD_tp5

µArray_HOD_tp5

Data validation: microarray data vs. real time RT-PCR data of potential self-renewal-related genes during osteogenesis for timepoints 5 (day+2), 7 (day+15), 8 (day+24)

µArray_HOD_tp7 µArray_HOD_tp8 RT-PCR_HOD_tp5

RT-PCR_HAD_tp7

-4

RT-PCR_HOD_tp7

log2 ratio Ref-TP

RT-PCR_HAD_tp8

2

4

6

8

10

-6

CD29

CD29

CD34

CD34

CD44

CD44

CD49b

CD49b

CD71

CD71

CD90/Thy-1

CD90/Thy-1

endoglin

endoglin

CD120a

CD120a

CD124

CD124

c-kit

c-kit

SCF (c-kit ligand)

SCF (c-kit ligand)

BMI1

BMI1

STRO1

STRO1

TDGF-1/cripto

TDGF-1/cripto

germ cell nuclear factor

germ cell nuclear factor

TEP1

TEP1

TERF1

TERF1

PTCH

Gene

Gene

-6

log2 ratio Ref-TP -2 0

PTCH

PTCH2

PTCH2

BCRP1

BCRP1

LIF

LIF

LIFR

LIFR

NODAL

NODAL

FOXD3

FOXD3

FOXG1

FOXG1

FOXH1

FOXH1

FOXM1

FOXM1

NOTCH1

NOTCH1

NOTCH2

NOTCH2

NOTCH3

NOTCH3

NOTCH4

NOTCH4

SOX5

SOX5

SOX6

SOX6

SOX9

SOX9

SOX12

SOX12

SOX15

SOX15

SOX17

SOX17

-4

-2

0

RT-PCR_HOD_tp8

2

4

6

8

10

Additional file 2 (page 4) Data validation: microarray data vs. real time RT-PCR data of selected genes during adipogenesis for timepoints 5 (day+2), 7 (day+10), 8 (day+15)

µArray_HAD_tp5 µArray_HAD_tp7 µArray_HAD_tp8 RT-PCR_HAD_tp5

Data validation: microarray data vs. real time RT-PCR data of selected genes during osteogenesis for timepoints 5 (day+2), 7 (day+15), 8 (day+24)

-4

-2

log2 ratio Ref-TP 0

4

6

8

10

-6

SNK

SNK

CD55

CD55

FLJ14525

FLJ14525

TACC1

TACC1

TBC1D2

TBC1D2

SORT1

SORT1

SLUG

SLUG

KG62

KG62

TGFBR3

TGFBR3

DDIT4

DDIT4

DSCD75

DSCD75

MKNK2

MKNK2

DSCR1

SDF1

Gene

Gene

SDF1 VEGF-A

µArray_HOD_tp8 RT-PCR_HOD_tp5

RT-PCR_HOD_tp8

log2 ratio Ref-TP

RT-PCR_HAD_tp8

2

µArray_HOD_tp7

RT-PCR_HOD_tp7

RT-PCR_HAD_tp7

-6

µArray_HOD_tp5

VEGF-A DSCR1

TRIP13

TRIP13

FOXM1

FOXM1

AXL

AXL

TACC3

TACC3

TFPI2

TFPI2

STMN1

STMN1

PTTG1

PTTG1

PODXL

PODXL

FN14

FN14

MGC11266

MGC11266

KIAA1363

KIAA1363

MGC5576

MGC5576

KIAA0746

KIAA0746

FLJ11029

FLJ11029

-4

-2

0

2

4

6

8

10