Diabetologia (2014) 57:347–351 DOI 10.1007/s00125-013-3097-4
SHORT COMMUNICATION
Adipose tissue INSR splicing in humans associates with fasting insulin level and is regulated by weight loss Dorota Kaminska & Maija Hämäläinen & Henna Cederberg & Pirjo Käkelä & Sari Venesmaa & Pekka Miettinen & Imre Ilves & Karl-Heinz Herzig & Marjukka Kolehmainen & Leila Karhunen & Johanna Kuusisto & Helena Gylling & Markku Laakso & Jussi Pihlajamäki
Received: 5 August 2013 / Accepted: 9 October 2013 / Published online: 7 November 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Aims/hypothesis The insulin receptor (INSR) has two protein isoforms based on alternative splicing of exon 11. INSR-A promotes cell growth whereas INSR-B predominantly regulates glucose homeostasis. In this study we investigated whether weight loss regulates INSR alternative splicing and the expression of splicing factors in adipose tissue. Methods To determine the relative ratio of the INSR splice variants, we implemented the PCR-capillary electrophoresis method with adipose tissue samples from two weight-lossintervention studies, the Kuopio Obesity Surgery study (KOBS, n = 108) and a very low calorie diet (VLCD)
Electronic supplementary material The online version of this article (doi:10.1007/s00125-013-3097-4) contains supplementary material, which is available to authorized users. D. Kaminska : M. Hämäläinen : M. Kolehmainen : L. Karhunen : H. Gylling : J. Pihlajamäki (*) Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, 70210 Kuopio, Finland e-mail:
[email protected] H. Cederberg : J. Kuusisto : M. Laakso Department of Medicine, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland P. Käkelä : S. Venesmaa : P. Miettinen : I. Ilves Department of Surgery, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland K.
