Open Forum Infectious Diseases Advance Access published October 15, 2016
Altered Monocyte and Endothelial Cell Adhesion Molecule Expression is linked to Vascular Inflammation in HIV-infection Manjusha Kulkarni1, Emily Bowman1, Janelle Gabriel1, Taylor Amburgy1, Elizabeth
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Mayne2, David A. Zidar3, Courtney Maierhofer4, Abigail Norris Turner4, Jose A. Bazan4, Susan L. Koletar4, Michael M. Lederman5, Scott F. Sieg5, Nicholas T. Funderburg1
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Ohio State University, Columbus, Ohio, USA
National Health Laboratory Service and Faculty of Health Sciences, University of
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Witwatersrand , Johannesburg 3
Harrington Heart & Vascular Institute, University Hospitals Case Medical Center
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Cleveland, OH, USA 4
Department of Medicine, Division of Infectious Diseases, Ohio State University,
Columbus, OH, USA
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Department of Internal Medicine, Division of Infectious Diseases, Case Western
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Reserve University/University Hospitals of Cleveland, Cleveland, OH, USA
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Keywords: HIV, monocytes, adhesion molecules, fractalkine, Lipoprotein-associated phospholipase A2
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Running Title: Monocyte adhesion and vascular inflammation
Corresponding Author
Nicholas Funderburg 453 W. 10th Ave. 535A Atwell Hall © The Author 2016. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact
[email protected].
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School of Health and Rehabilitation Sciences, Division of Medical Laboratory Science,
Columbus OH, 43210 Fax: 614 292 0210 Phone: 614 292 7303
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Email:
[email protected]
Total Word Count: 3484 words Total Abstract Count: 194 words
2 Supplemental Tables
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Number of References: 49
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Number of Inserts: 4 Figures, 1 Table
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Abstract
Background: HIV-infected individuals have increased risk for vascular thrombosis, potentially driven by interactions between activated leukocytes and the endothelium.
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Methods: Monocyte subsets (CD14+CD16-, CD14+CD16+, CD14DimCD16+) from HIV-
and antiretroviral therapy (ART)-treated HIV+ participants (N=19 and 49) were analyzed
by flow cytometry for adhesion molecule expression (LFA-1, Mac-1, CD11c/CD18, VLA4) and the fractalkine receptor (CX3CR1); these receptors recognize ligands (ICAMs,
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Plasma markers of monocyte (sCD14, sCD163) and EC (VCAM-1, ICAM-1,2,
inflammation were measured by ELISA.
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fractalkine) activation and systemic (TNFR-I, TNFR-II) and vascular (Lp-PLA2)
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Results: Proportions of CD16+ monocyte subsets were increased in HIV+ participants. Among all monocyte subsets, levels of LFA-1 were increased and CX3CR1 levels were
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decreased in HIV+ participants (p
