1. Patologia experimental â Congressos. I. Araújo, Eduardo José de Almeida. II. ..... EVALUATION OF rs11781889 POLIMORPHYSM OF NKX3.1 GENE AND ...
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Universidade Estadual de Londrina Graduate Program in Experimental Pathology
Editorial Board Alessandra Lourenço Cecchini Armani – Chair Eduardo José de Almeida Araújo – Chair Gláucia Eloisa Munhoz de Lion Siervo Glauco Akelinghton Freire Vitiello Kleber Paiva Trugilo Paulo da Silva Watanabe Victor Fattori (Universidade Estadual de Londrina)
Responsible for publishing: UNIVERSIDADE ESTADUAL DE LONDRINA PROGRAMA DE PÓS-GRADUAÇÃO EM PATOLOGIA EXPERIMENTAL Rodovia Celso Garcia Cid. PR445 Km 380, Campus Universitário / CEP: 86057-970 LONDRINA / PR / BRAZIL Contact: Eduardo José de Almeida Araújo / Alessandra Lourenço Cecchini Armani Telephone: +55 43 3371-5498 / +55 43 3371-4387
Graduate Program in Experimental Pathology
Londrina-PR
1st Edition
p.1-186
Catalogação na publicação elaborada pela Divisão de Processos Técnicos da Biblioteca Central da Universidade Estadual de Londrina. Dados Internacionais de Catalogação-na-Publicação (CIP)
I61a International Symposium of Experimental Pathology (1. : 2016 : Londrina, PR) Annals [of the] I International Symposium of Experimental Pathology [e do] VI Simpósio de Patologia Experimental da UEL [livro eletrônico] / Editorial board: Eduardo José de Almeida Araújo... [ et al.]. – Londrina : UEL, 2016. 1 Livro digital. Vários autores. Textos em português e inglês. Inclui bibliografia. Disponível em: http://www.uel.br/eventos/simposiopato/ ISBN 978-85-7846-411-0 1. Patologia experimental – Congressos. I. Araújo, Eduardo José de Almeida. II. Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-graduação em Patologia Experimental. III. Queen Mary University of London. IV. Simpósio de Patologia Experimental da UEL (6. : 2016 : Londrina, PR). V. Título. VI. Annals [do] VI Simpósio de Patologia Experimental da UEL.
CDU 616-092
2016
SUMMARY ABSTRACTS.................................................................................................................................. 13 NEGLECTED DISEASES CHALLENGES IN PARACOCCIDIOIDOMYCOSIS: NON-REACTIVE IMMUNODIFFUSION................ 14 PHYSICAL TRAINING MODULATES NITRIC OXIDE PRODUCTION AND CYTOKINE IMPROVING CARDIOVASCULAR RESPONSE AND RESISTANCE TO INFECTION TO Trypanosoma cruzi ........... 15 Caryocar coriaceum EXERTS EFFECTS AGAINST PROMASTIGOTE FORMS OF Leishmania amazonensis ................................................................................................................................ 16 ANTI-AMASTIGOTE EFFECT OF DIFFERENT EXTRACTS OF Caryocar coriaceum, A BRAZILIAN CERRADO PLANT ......................................................................................................................... 17 INHIBITION OF Paracoccidioides brasiliensis GROWTH BY SOIL YEAST CRUDE EXTRACT ........... 18 NANOTECHNOLOGY IN THE TREATMENT OF TOXOPLASMOSIS: A REVIEW ............................... 19 ROSUVASTATIN INHIBIT INFECTION AND PROLIFERATION OF TACHYZOITES OF Toxoplasma gondii (RH STRAIN) IN HeLa CELLS .............................................................................................. 20 LYMPHOTROPIC VIRUS INFLUENCE OF HUMAN T CELL TYPE 1 IN PATIENTS COINFECTED WITH HELMINTH Strongyloides stercoralis - A SYSTEMATIC REVIEW .................................................. 21 MACROPHAGES ACTIVATED WITH CONCANAVALIN-A INCREASE LEISHMANICIDE ACTIVITY BY REACTIVE OXYGEN SPECIES PRODUCTION .................................................................................. 22 CONCANAVALIN-A INDUCES TNF-α AND NITRIC OXIDE PRODUCTION BY PERITONEAL CELLS PRETREATED IN EXPERIMENTAL LEISHMANIASIS ....................................................................... 23 ACUTE Toxoplasma gondii INFECTION ALTERS THE MYELOPEROXIDASE ACTIVITY IN RAT DUODENUM ................................................................................................................................ 24 COMBINED BENZNIDAZOLE AND ASPIRIN CHEMOTHERAPY IN MICE INFECTED WITH Trypanosoma cruzi ...................................................................................................................... 25 CROSSTALK BETWEEN CYCLOOXYGENASE PATHWAY AND ADENYLATE-CYCLASE IN THE REGULATION OF ANTI-Trypanosoma cruzi ACTIVITY BY HUMAN MONOCYTES ......................... 26 NON-COMMUNICABLE DISEASES HORMONE THERAPY ATTENUATES THE SYSTEMIC PROOXIDANT STATUS IN POSTMENOPAUSAL WOMEN....................................................................................................................................... 27 PROTECTIVE ASSOCIATION OF TGF-βR2 GENE POLYMORPHISM AND CYTOKINE PLASMA LEVELS IN CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA................................................................... 28 PROBUCOL ATTENUATES LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION BY REDUCING LEUKOCYTE RECRUITMENT, CYTOKINE PRODUCTION AND NF-КB ACTIVITY ............................. 29 HASHIMOTO’S THYROIDITIS: A REVIEW ARTICLE ........................................................................ 30 MUSCLE RECONDITIONING IN CHRONIC OBSTRUTIVE PULMONARY DISEASE ........................... 31
EXPERIMENTAL MODEL PADRONIZATION OF MURINE ORAL’S MELANOMA: PILOT STUDY ...... 32 iNOS PARTICIPATION IN CARDIOVASCULAR AND OXIDATIVE PARAMETERS OF FEMALE RATS WITH ENDOTOXEMIA INDUCED BY LPS ...................................................................................... 33 INVOLVEMENT OF NITRIC OXIDE SYNTHASE ISOFORMS IN CARDIOVASCULAR, AUTONOMIC AND OXIDATIVE ASPECTS IN FEMALE RATS ................................................................................ 34 GENETIC POLYMORPHISMS AND EXPRESSION OF FOXP3 mRNA IN AGGRESSIVE BREAST CANCER SUBTYPES ...................................................................................................................... 35 CYTOKINE PROFILE IN PATIENTS WITH PROGRESSIVE MULTIPLE SCLEROSIS AND ITS ASSOCIATION WITH DISEASE PROGRESSION AND DISABILITY .................................................... 36 HISTOPATHOLOGICAL EVALUATION FOR ABERRANT CRYPT FOCI (ACF) STUDY IN EXPERIMENTAL MODEL OF COLORECTAL CANCER IN INDUCED BY 1,2-DIMETILHIDRAZINA (DMH) IN RATS WISTAR............................................................................................................... 37 INTRAUTERINE AND LACTATIONAL EXPOSURE TO FLUOXETINE INHIBITED THE AORTIC ADAPTATION INDUCED BY ACUTE RESTRAIN STRESS IN RATS ................................................... 38 DAMAGE CAUSED BY YELLOW FEVER VACCINE ON THE GESTATIONAL DEVELOPMENT ........... 39 MATERNAL EXPOSURE TO METFORMIN DID NOT INTERFERE WITH VASCULAR ENDOTHELIAL FUNCTION OF ADULT MALE OFFSPRING..................................................................................... 40 GENE EXPRESSION ANALYSIS IN CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA: CHEMOTHERAPY INFLUENCE IN KEY REGULATING GENES ......................................................... 41 BARK ETHANOLIC EXTRACT FROM Spondias dulcis FORST F. PROTECTS AGAINST ALTERATIONS OF THE REDOX STATUS INDUCED BY CYCLOPHOSPHAMIDE AND BENZO[a]PYRENE IN MICE ... 42 THE VANILLIC ACID INHIBITS THE EHLICH-TUMOR CELLS INDUCED PAIN LIKE-BEHAVIOR ........ 43 POSSIBLE EFFECTS OF TGFB1 SIGNAL PEPTIDE AND PROMOTER REGION GENETIC POLYMORPHISMS AND HAPLOTYPE STRUCTURES: POSSIBLE ROLE AS A SUSCEPTIBILITY MARKER....................................................................................................................................... 44 VITAMIN D DEFICIENCY IS ASSOCIATED WITH ACUTE ISCHEMIC STROKE, C-REACTIVE PROTEIN, AND SHORT-TIME OUTCOME ...................................................................................................... 45 IMMUNE RESPONSE AND VIRULENCE FACTORS ASSOCIATED CANDIDIASIS IN DIABETES: A REVIEW ........................................................................................................................................ 46 EVALUATION OF METABOLIC AND CARDIOVASCULAR PARAMETERS OF MALE AND FEMALE RATS EXPOSED TO METFORMIN DURING GESTATIONAL AND LACTATION PERIOD ................... 47 MULTIPLE ENDOCRINE NEOPLASIA TYPE 2A: CASE REPORT ....................................................... 48 PROTECTIVE EFFECTS OF NICOTINAMIDE ON MICE OFFSPRING EXPOSED TO CYCLOPHOSPHAMIDE DURING PREGNANCY .............................................................................. 49 ALTERATIONS IN REPRODUCTIVE PARAMETERS OF MICE EXPOSED TO WINE DURING SPERMATOGENESIS ..................................................................................................................... 50 ANTIBIOTIC SUSCEPTIBILITY OF Staphylococcus aureus ISOLATED FROM BLOODSTREAM INFECTIONS IN A UNIVERSITY HOSPITAL SOUTH BRAZIL FOR A PERIODO OF FIFTEEN YEARS ... 51
EVALUATION OF PHYSICAL AND REFLEXOLOGICAL DEVELOPMENT OF MICE OFFSPRING EXPOSED TO PROPOFOL IN DIFFERENT GESTACIONAL PERÍODS ................................................ 52 NITRIC OXIDE-GLUTAMATE INTERACTIONS IN ROSTRAL VENTROLATERAL MEDULLA OF OBESE RATS: INVOLVEMENT OF iNOS ISOFORM.................................................................................... 53 SKELETAL MUSCLE WASTING PROMOTES LIPID PEROXIDATION AND SARCOPLASMIC RETICULUM ALTERATIONS IN A FIBER TYPE DEPENDENT MANNER ........................................... 54 KAURENOIC ACID REDUCES EOSINOPHIL RECRUITMENT IN ASTHMA BY REDUCING TH2 CYTOKINE PRODUCTION IN MICE................................................................................................ 55 MACROPHAGES-EXPRESSING FOXP3 TRANSCRIPTION FACTOR: DO THEY REALLY EXIST? ......... 56 EFFECT OF METFORMIN IN ACUTE MODEL OF MURINE UVB IRRADIATION AND THE INVOLVEMENT OF SYSTEMIC ROS............................................................................................... 57 ROLE OF ROS AND IMMUNE RESPONSE OF LANGERHANS CELL IN AN ACUTE UVB IRRADIATION MURINE MODEL TREATED WITH METFORMIN ........................................................................... 58 ALUMINIUM CHLORIDE, DURING THE PERIBUBERTAL PERIOD, AFFECT THE CYTOKINES LEVEL AND MORFOLOGY IN TESTES OF RATS........................................................................................ 59 SYSTEMIC OXIDATIVE STRESS PROFILE DURING THE PROGRESSION OF EXPERIMENTAL METASTASIS OF MURINE MELANOMA ....................................................................................... 60 SYSTEMIC OXIDATIVE STRESS PROFILE DURING THE PROGRESSION OF EXPERIMENTAL METASTASIS OF MURINE MELANOMA ....................................................................................... 61 CYTOTOXICITY EFFECT IN TM3 CELL LINE (LEYDIG CELL) CAUSED BY BPA (BISPHENOL A) ......... 62 TANSFORMING GROWTH FACTOR TYPE 2 RECPTOR (TGFβR2) PROMOTER REGION POLYMORPHISM EXERT SUBTYPE SPECIFIC ROLES IN BREAST CANCER ..................................... 63 THE ROLE OF TRANSFORMING GROWTH FACTOR BETA 1 (TGFB1) FUNCTIONAL POLYMORPHISMS IN GENE EXPRESSION AND CYTOKINE PRODUCTION .................................... 64 METFORMIN REGULATES SKIN REDOX PROFILE IN UVB-INDUCED SQUAMOUS SKIN CELL CARCINOMA ................................................................................................................................ 65 Rhopalurus rochai VENOM-INDUCED INFLAMMATORY PAIN, OVERT-PAIN LIKE BEHAVIOR DEPENDS ON CELL RECRUITMENT AND CYTOKINES PRODUCTION ............................................ 66 Tityus serrulatus VENOM INDUCES INFLAMMATORY PAIN IN MICE .......................................... 67 MALIGNANT MELANOMA SKIN ................................................................................................... 68 OBSTRUCTIVE PULMONARY DISEASE: PATHOPHYSIOLOGY AND REHABILITATION PULMONARY ..................................................................................................................................................... 69 DOES MATERNAL EXPOSURE TO FLUOXETINE INDUCE METABOLIC ALTERATIONS IN ADULT MALE OFFSPRING? ...................................................................................................................... 70 KAURENOIC ACID REDUCES DICLOFENAC-INDUCED ACUTE KIDNEY INJURY THROUGH INHIBITION OF OXIDATIVE STRESS IN MICE ................................................................................ 71 EVALUATION OF BIOCHEMICAL PARAMETERS OF DIABETICS RATS SUBMITTED TO FOOD RESTRICTION DIET ....................................................................................................................... 72
CITRAL HAS CYTOTOXIC ACTION IN MURINE MELANOMA CELLS (B16F10) BY GENERATING REACTIVE OXYGEN SPECIES ......................................................................................................... 73 EFFECTS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS .................................................................. 74 LATEX C-SERUM FROM Hevea brasiliensis INDUCES NECROTIC CELL DEATH IN MURINE MELANOMA CELL LINE (B16F10)................................................................................................. 75 CLINICAL-EPIDEMIOLOGICAL CHARACTERIZATION AND EVALUATION OF ERYTHROCYTIC ANTIOXIDANT DEFENSES OF PATIENTS AFFECTED BY BASAL CELL CARCINOMA IN THE NORTHERN REGION OF PARANA ................................................................................................ 76 CELL ADHESION MOLECULES EVALUATION AS PREDICTORS OF DIAGNOSIS AND DISEASE ACTIVITY IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS. ............................................ 77 BARK ETHANOLIC EXTRACT FROM Spondias dulcis FORST F. PROTECTS AGAINST ALTERATIONS OF THE REDOX STATUS INDUCED BY CYCLOPHOSPHAMIDE AND BENZO[a]PYRENE IN MICE ... 78 THE PREDICTIVE VALUE OF TRANSFORMING GROWTH FACTOR-β IN WILMS TUMOR IMMUNOPATHOGENESIS ............................................................................................................ 79 TREATMENT WITH PIOGLITAZONE AND INSULIN IN WALKER-256 TUMOR BEARING RATS: EFFECTS ON PANCREATIC ISLETS................................................................................................. 80 CINNAMON, TURMERIC AND OKRA: EFFECTS OF DIET ON BODY WEIGHT AND BIOCHEMICAL PARAMETERS IN WISTAR RATS ................................................................................................... 81 Hypericum perforatum REDUCES INFLAMMATORY PAIN IN MICE ............................................. 82 CLINICAL-EPIDEMIOLOGICAL CHARACTERIZATION OF PATIENTS AFFECTED BY SQUAMOUS CELL CARCINOMA ALONE AND ASSOCIATED WITH OTHERS SKIN NEOPLASIAS IN THE NORTHERN REGION OF PARANA .................................................................................................................... 83 SIM2 AS A RNA CIRCULANTING MARKER FOR PROSTATE CANCER DIAGNOSIS AND PROGNOSIS ..................................................................................................................................................... 84 Tityus bahiensis VENOM INDUCES INFLAMMATORY PAIN IN MICE ........................................... 85 ANALGESIC AND ANTI-INFLAMMATORY EFFECT OF NARINGENIN IN TITANIUM DIOXIDEINDUCED ARTHRITS ..................................................................................................................... 86 CLINICAL-EPIDEMIOLOGICAL CHARACTERIZATION OF PATIENTS AFFECTED BY MULTIPLE SKIN NEOPLASIAS IN THE NORTHERN REGION OF PARANA. ............................................................... 88 EVALUATION OF rs11781889 POLIMORPHYSM OF NKX3.1 GENE AND PROSTATE CANCER RISK ..................................................................................................................................................... 89 POLYMORPHISM ANALYSIS OF GENE AR IN PATIENTS WITH PROSTATE CANCER...................... 90 INVERTED PAPILLOMA. CASE REPORT ........................................................................................ 91 CLINICO-PATHOLOGICAL PARAMETERS INVOLVED IN THE ASSOCIATION BETWEEN DIFFERENTIATED THYROID CANCER AND HASHIMOTO THYROIDITIS: A REVISION OF THE LITERATURE ................................................................................................................................. 92
INDUCTION OF LIPID PEROXIDATION AND OXIDATIVE INJURY OF DNA BY CURCUMIN IN HUMAN BREAST CANCER CELLS MCF7 AND MDA-MB-231 ........................................................ 93 SEARCH OF HUMAN MAMMARY TUMOUR VIRUS (MMTV-LIKE) DNA IN BREAST CANCER MICROENVIROMENT ................................................................................................................... 94 OXIDATIVE AND NITROSATIVE STRESS AND AUTOANTIBODIES ARE ASSOCIATED WITH LUPUS NEPHRITIS.................................................................................................................................... 95 CREATINE SUPPLEMENTATION INCREASES LIVER OXIDATIVE STRESS AND LIPIDS ACCUMULATION IN A MODEL OF MURINE ALCOHOLIC STEATOSIS ........................................... 96 METFORMIN PRETREATMENT PREVENTS DOXORUBICIN RESISTANCE INDUCTION IN MCF-7 AND MDA-MB-231 HUMAN BREAST CANCER CELLS THROUGH OXIDATIVE STRESS GENERATION, INDUCTION OF P53 AND TGF-Β1 AND DECREASE OF NRF2 ................................ 97 COLON HISTOPATOLOGICAL EVALUATION OF RATS WISTAR FOR ABERRANT CRYPT FOCI (ACF) STUDY AND ADENOMATOUS INTESTINAL TUMOR ..................................................................... 98 VINPOCETINE AS ANTI-INFLAMMATORY AGENT – A REVIEW .................................................... 99 ALBUMIN AND PROTEIN OXIDATION ARE PREDICTORS THAT DIFFERENTIATE RELAPSINGREMITTING FROM PROGRESSIVE CLINICAL FORMS OF MULTIPLE SCLEROSIS ......................... 100 EVALUATION OF ANTINFLAMMATORY AND ANTIOXIDANT EFFECTS OF HESPERIDIN-METHYLCHALCONE IN LPS-INDUCED ACUTE LUNG INJURY IN MICE. .................................................... 101 EVALUATION OF OCCUPATIONAL EXPOSURE AT PESTICIDES USING BIOCHEMICAL, GENOTOXIC AND OXIDATIVE MARKERS ........................................................................................................ 102 VINPOCETINE AMELIORATES ACETIC ACID-INDUCED COLITIS BY INHIBITING OXIDATIVE STRESS AND PRO-INFLAMMATORY CYTOKINES PRODUCTION IN MICE ............................................... 103 SYSTEMIC CHANGES IN THE REDOX STATUS IN A MURINE MODEL OF UVB-INDUCED NONMELANOMA SKIN CANCER ................................................................................................ 104 CLINICAL-EPIDEMIOLOGICAL CHARACTERIZATION AND EVALUATION OF ERYTHROCYTIC ANTIOXIDANT DEFENSES OF PATIENTS AFFECTED BY ACTINIC KERATOSIS AND NONMELANOMA SKIN CANCER IN THE NORTHERN REGION OF PARANA. ........................................................... 105 EFFECTS OF SLEEP RESTRICITON DURING PERIPUBERTY ON TESTICULAR DEVELOPMENT OF RATS .......................................................................................................................................... 106 INFLAMMATION AND OXIDATIVE STRESS IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS WITH METABOLIC SYNDROME – A REVIEW .............................................................................. 107 COMMUNICABLE DISEASES LEPROSY IN PRUDENTÓPOLIS: THE PORTRAIT OF ONE OF PARANÁ'S INLAND CITIES .............. 108 P2 ALLELE OF PVUII GENETIC POLYMORPHISM OF LOW-DENSITY LIPOPROTEIN RECEPTOR IS ASSOCIATED WITH INCREASED LIPID PEROXIDATION AND LIVER INJURY IN CHRONIC HEPATITIS C PATIENTS ................................................................................................................................ 109 THE PROFILE OF OXIDATIVE STRESS MARKERS IS DEPENDENT ON VITAMIN D LEVELS IN PATIENTS WITH CHRONIC HEPATITIS C ..................................................................................... 110
POLYMORPHISM rs3087465 IN THE TGFBR2 GENE IS ASSOCIATED WITH HUMAN PAPILLOMAVIRUS INFECTION ................................................................................................... 111 SYPHILIS: MOTHER TO SON ....................................................................................................... 112 THE INFLUENCE OF TGFB1 POLYMORPHISM RS1800469 (C.-1347C>T) IN THE HPV INFECTION AND CERVICAL LESIONS IMMUNOPATHOGENESIS ................................................................... 113 TGFB1 SIGNAL PEPTIDE POLYMORPHISMS INFLUENCE HPV INFECTION AND CERVICAL LESIONS DEVELOPMENT .......................................................................................................................... 114 INTESTINAL INFLAMMATION GENERATED BY TOXOPLASMOSIS PROMOTE LOSS NEURONAL AND GLIAL POPULATION OF ILEUM SUBMUCOSAL PLEXUS OF RATS Wistar ........................... 115 COINFECTION BETWEEN HPV AND Chlamydia trachomatis AND ITS ROLE IN CERVICAL LESIONS DEVELOPMENT .......................................................................................................................... 116 ANTIBODIES IgY ANTI-NP OF THE VIRUS H1N1 FOR DEVELOPMENT OF DIAGNOSTIC TESTS FOR THE DETECTION OF THE INFLUENZA VIRUS .............................................................................. 117 INFLUENCE OF CXCL12 RS1801157 POLYMORPHISM IN HPV INFECTION AND LESION DEVELOPMENT .......................................................................................................................... 118 GENETIC AND NON GENETIC FACTORS IN THE ETIOLOGY OF CARIES IN CHILD PRESCHOOL ... 119 PHYSIOLOGICAL AND MOLECULAR CHARACTERISTICS OF CARBAPENEM RESISTANCE IN Klebsiella pneumoniae AND Enterobacter aerogenes .............................................................. 120 PLACENTAL HISTOPATHOLOGIC ALTERATIONS AS A TOOL IN CONGENITAL ZIKA VIRUS INFECTION DIAGNOSIS: A CASE REPORT ................................................................................... 121 CYTOKINE PROFILE FOLLOWING INTRAPERITONEAL INFECTION BY Escherichia coli ENTEROHEMORRHAGIC IN SWISS MICE ................................................................................... 122 EXPANDED ABSTRACTS ............................................................................................................ 123 CARACTERIZAÇÃO DE POLIMORFISMOS DO TGFB1 E EXPRESSÃODO TGFB1, TGFBR1 e TGFBR2 EM PACIENTES COM CÂNCER CERVICAL ................................................................................... 124 AVALIAÇÃO DOS EFEITOS DA DIETA HIPERLIPÍDICA ISOLADA OU ASSOCIADA AO HIPOTIREOIDISMO E HIPERTIREOIDISMO SOBRE O TESTÍCULO E EPIDÍDIMO DE CAMUNONGOS C57BL/6: ANÁLISES MORFOFISIOLÓGICAS E MOLECULARES .................................................... 128 AVALIAÇÃO DA PERFORMANCE REPRODUTIVA DE CAMUNDONGOS EXPOSTOS AO CLORIDRATO DE BUPROPIONA DURANTE A ESPERMATOGÊNESE E DOS POSSIVEIS EFEITOS TERATOGÊNICOS, DESENVOLVIMENTO PÓS NATAL E COMPORTAMENTAIS NA PROLE .......... 132 AVALIAÇÃO DOS EFEITOS DO INSETICIDA MALATION SOBRE O DESENVOLVIMENTO DO SISTEMA GENITAL MASCULINO DE RATOS DESDE O PERÍODO JUVENIL ATÉ A PUBERDADE ... 135 O PAPEL DE AGRECANAS EM DISTÚRBIOS GASTROINTESTINAIS DE HUMANOS E CAMUNDONGOS ....................................................................................................................... 139 O PAPEL DE FOSFACANAS EM DISTÚRBIOS GASTROINTESTINAIS DE HUMANOS E CAMUNDONGOS ....................................................................................................................... 143
ANÁLISE DO CÓLON DE RATOS SUBMETIDOS A DIFERENTE DURAÇÕES DE INFECÇÃO CAUSADA POR Toxoplasma gondii............................................................................................................. 147 ESTUDO COMPARATIVO DA FISIOPATOLOGIA NO ESCORPIONISMO INDUZIDO POR PEÇONHAS DOS Tityus serrulatus E Rhopalurus rochai ............................................................................... 150 ESTABELECIMENTO DA DIFERENÇA CRITICA EM PARÂMETROS DE ESTRESSE OXIDATIVO UTILIZANDO PROTOCOLO DE EXERCÍCIO EXCÊNTRICO............................................................. 153 ESTRESSE OXIDATIVO E PERFIL INFLAMATÓRIO SISTÊMICO NA DOENÇA ARTERIAL OCLUSIVA PERIFÉRICA (DAOP) EM HUMANOS .......................................................................................... 157 ESTUDO DOS NÍVEIS DE VITAMINA D E POLIMORFISMO VRD-FOKI EM PACIENTES COM CÂNCER COLORRETAL: CORRELAÇÃO COM O ESTADIAMENTO DA DOENÇA. ........................................ 161 AVALIAÇÃO DA REATIVAÇÃO DO CITOMEGALOVÍRUS EM PACIENTES COM SEPSE BACTERIANA EM UNIDADES DE TERAPIA INTENSIVA ..................................................................................... 165 AVALIAÇÃO DOS EFEITOS ESPINAIS DO MEDIADOR LIPÍDICO PRÓ-RESOLUÇÃO RESOLVINA D1 EM MODELO DE GOTA INDUZIDA POR CRISTAIS DE MONOURATO DE SÓDIO (MSU) ............. 169 AVALIAÇÃO DOS EFEITOS ESPINAIS DO MEDIADOR LIPÍDICO PRÓ RESOLUÇÃO RESOLVINA D2 EM MODELO DE ARTRITE GOTOSA INDUZIDA POR CRISTAIS DE MONOURATO DE SÓDIO (MSU) ................................................................................................................................................... 173 AVALIAÇÃO DO EFEITO ANTINOCICEPTIVO E ATIVIDADE ANTIOXIDANTE DO DIOSMIN EM MODELO DE DOR NEUROPÁTICA INDUZIDA PELA CONSTRIÇÃO DO NERVO CIÁTICO EM CAMUNDONGOS ....................................................................................................................... 177 INDEX......................................................................................................................................... 181
PROGRAM
ABSTRACTS
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CHALLENGES IN PARACOCCIDIOIDOMYCOSIS: NON-REACTIVE IMMUNODIFFUSION Adriane Lenhard-Vidal1, João Paulo Assolini1, Nilson de Jesus Carlos1, Eiko Nakagawa Itano1 1 Laboratório
de Imunologia Aplicada, Depto de Ciências Patológicas/CCB, Universidade Estadual de Londrina, Rodovia Celso Garcia Cida (PR-445), km 380, 86057-970 Londrina, PR, Brasil.
Introduction: Paracoccidioidomycosis (PCM) is caused by the thermodimorphic fungi Paracoccidioides spp. In the Brazilian public health system, its diagnosis relies on the use of immunodiffusion (ID), that despite having low costs and being easy to carry out, has lower sensibility than other tests. Objective: to evaluate the rate of misdiagnosis of PCM by the use of ID. Material and methods: Current research was approved by the Internal Scientific Commission and the Research Bioethics Committee of the State University of Londrina (n. 33455 2011.06, CAEE 0314.0.268.000-11). The 32 sera were from patients already clinically diagnosed, living in the northern and west regions of Paraná State, Brazil (HC-HURNP-COU UEL; Municipal Laboratory Foz do Iguaçu; HC UNIOESTE). Cell free antigens (CFA) and exoantigens (ExoAg) were produced from P. brasiliensis strain B339. The ID tests were performed in duplicates, with the antigens in the central position and titration of the sera (pure and up to 1/32) in the surrounding wells (25 μL/well). In addition, IgG was detected by indirect ELISA (CFA 25 µg/mL, ExoAg 1/4000). Results: Ten samples (31%) were negative in ID, out of which seven were negative in both CFA and ExoAg ID. If ELISA was co-employed as part of the diagnosis, these patients would be considered positive, but the majority (70%) had low or intermediate titers/optical density readings. In addition to the difficulty to compare results among institutions due to the lack of antigen standardization (strain, production, concentration, etc.), the employment of solely ID is another obstacle. The use of a second immunological method when ID turns out negative seems advisable. Conclusion: There would be a high rate of false negative results if only ID was employed. Although ID may be cost-effective and easy to perform in any laboratory, it is necessary to investigate other more sensitive methods.
Keywords: ELISA Immunodiagnosis; Immunoenzymatic assay. Grants: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Araucária/PR and Pró-reitorias de Pós-graduação e Extensão from Londrina State University (PROPPG/PROEX/UEL). Thematic area: Neglected diseases.
14
PHYSICAL TRAINING MODULATES NITRIC OXIDE PRODUCTION AND CYTOKINE IMPROVING CARDIOVASCULAR RESPONSE AND RESISTANCE TO INFECTION TO Trypanosoma cruzi Bruno Fernando Cruz Lucchetti1, Nágela Ghabdan Zanluqui1, Hiviny de Ataides Raquel1, Maria Isabel Lovo-Martins1, Vera Lúcia Hideko Tatakihara1, Mônica de Oliveira Belém 1, Lisete Compagno Michelin2, Eduardo José de Almeida Araújo1, Phileno Pinge-Filho1, Marli Cardoso Martins Pinge1. 1Universidade 2Universidade
Estadual de Londrina, Londrina-PR, de São Paulo, São Paulo-SP
Introduction: Trypanosoma cruzi infection causes innate immune response initiated in the acute phase responsible for host resistance to the parasite. It is observed progressive heart inflammation, fibrosis with changes in the architecture and functionality of the heart. Recent studies have shown that chronic physical activity (PA) of moderate intensity can act as a resistance factor against T. cruzi infection in animals. However, the cardiovascular parameters of mean arterial pressure and heart rate (HR), and nitric oxide levels (NO) accompanying the acute phase of infection in animals previously trained, are not clear. Objective: Evaluate the effects of previous physical conditioning on inflammatory, cardiovascular and biochemical aspects of resistance to infection by T. cruzi. Material and methods: Study approved by CEUA:28105.2014.72. Swiss mice were randomized into four groups: control sedentary, control trained, sedentary infected (SI), and trained infected (TI). Physical activity was performed on a treadmill for nine weeks. After PA mice were infected with 5x10³ trypomastigotes of T. cruzi (y strain). Results: We observe resting bradycardia and better performance in trained animals compared to sedentary. On the 20th day post-infection (dpi), we found a decrease in HR in SI compared with TI animals (699.73±42.37vs742.11±25.35bpm). There was also an increase in NO production in heart tissue in 20 dpi in SI, and normalized in the TI group (20.73±2.74vs6.51±1.19). An increase in the plasma concentration of pro-inflammatory cytokines (IL12, TNF-α, IFN-γ,) and MCP-1 was found in the SI group, compared to TI. An increase in parasitaemia in 15 and 17 dpi in the SI group, since the TI group this increase was attenuated. In addition, TI animals showed increased survival compared to the SI. Conclusion: Our results suggest that previous PA plays a preventive role in resistance to infection by T. cruzi, modulating the inflammatory response and NO levels in infected mice.
Keywords: Chagas disease; Inflammation; Arterial pressure; Heart rate Grants: CAPES and CNPq. Thematic area: Neglected diseases.
15
Caryocar coriaceum EXERTS EFFECTS AGAINST PROMASTIGOTE FORMS OF Leishmania amazonensis Fernanda Tomiotto-Pellissier1; Daniela Ribeiro Alves2; Selene Maia de Morais 2, Milena Menegazzo Miranda-Sapla1, Bruna Taciane da Silva Bortoleti1, João Paulo Assolini1, Manoela Daiele Gonçalves1, Virginia Marcia Concato1, Cláudia Stoeglehner Sahd1, Alan Ferreira Chagas1, Idesania Nazareth Costa1, Ivete Conchon-Costa1, Wander Rogério Pavanelli1. 1 State 2
University of Londrina, Londrina 86057-970, Paraná, Brazil State University of Ceará, Fortaleza 60.714.903, Ceará, Brazil
Introduction: Leishmaniasis is caused by species of the protozoa Leishmania. The lack of effective drugs and the side effects, coupled with the inability of host to eliminate the parasite, have been the driving force behind the search for new chemotherapeutic agents. Plants of Caryocar genus are widely distributed Brazilian cerrado trees, that have been used as food and popular medicine. Studies have demonstrated several biological effects of Caryocar sp., as activity against bacteria, fungi, protozoa and tumor cells. Objective: In this sense, the aim of the present study was to verify the leishmanicide effect of extracts from different parts of the plant C. coriaceum on promastigotes forms of L. amazonensis. Material and methods: It was used different plant extracts, according to the origin and solvent, respectively: (1) fruit pulp, ethanol; (2) fruit peel, ethanol; (3) leaf, ethyl acetate. The extracts were tested at 0.05 mg/mL. Promastigotes forms of L. amazonensis were maintained in 199 medium. The antipromastigote assay was performed through neubauer chamber counting and samples were analyzed by Scanning Electron Microscopy (SEM). Results: Our data showed that all the tested extracts were able to reduce the amount of viable pomastigotes after 24 (pT polymorphism in a recessive manner with increased susceptibility to TW (OR: 10.68; CI: 3.685 to 30.95; pT polymorphism in a recessive manner, showed a tendency to risk (OR: 2.800; CI: 0.9692 to 8.089, p:0.0644). Was observed a significant difference in the distribution of haplotype structures between control children and WT (p:0.001), whose haplotype GCTG was associated with protection (OR: 0.2361 CI: 0.1045 to 0.0534; p:0.0002) and GTTG haplotype associated with susceptibility to WT (OR: 12.00, Cl: 4.202 to 34.27; p
