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and Synovial Fluid-Derived Mesenchymal Stem

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Jan 18, 2017 - of fluorescence were measured using the BD FACS Diva software ..... Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, et al. ... Prado AA, Favaron PO, da Silva LC, Baccarin RY, Miglino MA, Maria. DA.
Original Research published: 18 January 2017 doi: 10.3389/fvets.2016.00121

Donor-Matched comparison of chondrogenic Potential of equine Bone Marrow- and synovial FluidDerived Mesenchymal stem cells: implications for cartilage Tissue regeneration Mohammed Zayed1,2, Christopher Caniglia3, Nabil Misk4 and Madhu S. Dhar1*  Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA,  Department of Animal Surgery, College of Veterinary Medicine, South Valley University, Qena, Egypt, 3Manor Equine Hospital, Monkton, MD, USA, 4 Department of Animal Surgery, College of Veterinary Medicine, Assiut University, Asyut, Egypt 1 2

Edited by: Debbie Guest, Animal Health Trust, UK Reviewed by: Rebeca Blázquez Durán, Centro de Cirugía de Mínima Invasión Jesús Usón, Spain Helen Elizabeth McCarthy, Cardiff University, UK *Correspondence: Madhu S. Dhar [email protected] Specialty section: This article was submitted to Veterinary Regenerative Medicine, a section of the journal Frontiers in Veterinary Science Received: 12 September 2016 Accepted: 19 December 2016 Published: 18 January 2017 Citation: Zayed M, Caniglia C, Misk N and Dhar MS (2017) Donor-Matched Comparison of Chondrogenic Potential of Equine Bone Marrowand Synovial Fluid-Derived Mesenchymal Stem Cells: Implications for Cartilage Tissue Regeneration. Front. Vet. Sci. 3:121. doi: 10.3389/fvets.2016.00121

Mesenchymal stem cells (MSCs) have been demonstrated to be useful for cartilage tissue regeneration. Bone marrow (BM) and synovial fluid (SF) are promising sources for MSCs to be used in cartilage regeneration. In order to improve the clinical outcomes, it is recommended that prior to clinical use, the cellular properties and, specifically, their chondrogenic potential must be investigated. The purpose of this study is to compare and better understand the in vitro chondrogenic potential of equine bone marrow-derived mesenchymal stem cells (BMMSCs) and synovial fluid-derived mesenchymal stem cells (SFMSCs) populated from the same equine donor. BM- and SF-derived MSCs cultures were generated from five equine donors, and the MSCs were evaluated in vitro for their morphology, proliferation, trilineage differentiation, and immunophenotyping. Differences in their chondrogenic potentials were further evaluated quantitatively using glycosaminoglycan (GAG) content and via immunofluorescence of chondrogenic differentiation protein markers, SRY-type HMG box9, Aggrecan, and collagen II. The BMMSCs and SFMSCs were similar in cellular morphology, viability, and immunophenotype, but, varied in their chondrogenic potential, and expression of the key chondrogenic proteins. The SFMSCs exhibited a significant increase in GAG content compared to the BMMSCs (P