e-Proceeding of 1st International Nastaran Cancer Symposium-2015 www.nastaransymposium.com/proceeding2015
Anti-metastatic effect of Polysaccharide Extracted from Brittle star Ophiocoma erinaceus on human cervical cancer cells in vitro Farzaneh Salek_Abdollahi 1 , Javad Baharara 2 , Najme Nikdel 3 , Elaheh Amini4 1. Faculty of Biological Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran 2. Department of Biology, Research Center for Applied Bology, Mashhad Branch, Islamic Azad University, Mashhad, IranFaculty of Biological Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran 3. Department of Aniimal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Abstract Cervical cancer is one of the most common causes of gynecologic malignancies around the world. Unfortunately, there are severe side effects and chemo-resistance that limit the use of drugs in cancer treatment. Brittle stars are the most abundant group of echinoderm related to sea star which contains numerous bioactive metabolites. In the present study, we evaluated anti-metastatic effect of polysaccharide extracted from the brittle star on HeLa cancer cells. HeLa cells were cultured in RPMI 1640 medium supplemented with 10% FBS, 1% antibiotic. After overnight, cells were treated with brittle star organic extract at different concentrations (0 to 200 μg) and cultured for 24, and 48 h. Then MTT assay, DAPI, Acridine orange / propodium iodide staining and scratch assay was performed. Based on biological assays, isolated polysaccharide from brittle star suppressed HeLa cell growth with IC50 concentration of (40 µg/ml) mostly through apoptotic recruitment. In vitro scratch assay exhibited that cell migration was successfully blocked up to 40 µg/ml of extracted polysaccharide which indicating anti-metastatic effect of brittle star isolated polysaccharide on cervical cancer cells. Consequently, extracted polysaccharide from brittle star exerted cytotoxicity on cervical cancer cells can potentially prevent cervical cancer progression and migration which demonstrate anti-cancer efficacy of marine bioactive compounds. Keywords: Cervical cancer,Apoptosis, Polysaccharide,Brittle star, Metastasis Corresponding Author: Elaheh Amini Email:
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