Aug 23, 2012 ... Cryptococcus sp. – all drugs. M27-A3. • Drug Preparation. • AMB. • DMSO ... MIC
testing methods (typically 2 dilutions above the modal MIC).
Apr 1, 2008 - Reference Method for Broth Dilution. Antifungal Susceptibility Testing of Yeasts;. Approved StandardâThird Edition. This document addresses ...
Apr 1, 2008 - studies are included in the current M27-A3 and M27-S3 (Informational Supplement)13 documents. Key Words antifungal, broth macrodilution ...
Mar 31, 2003 - resco, Solon, Ohio), while itraconazole was prepared in poly- ethylene glycol (Sigma Biochemical Company) (molecular weight. 8000); all of ...
Jul 5, 2006 - compare results of antifungal susceptibility testing of fungal and filamentous ... *Corresponding author (email: ).
Mar 13, 2002 - Denning, D. W., S. A. Radford, K. L. Oakley, L. Hall, E. M. Johnson, and ... Espinel-Ingroff, A., M. Bartlett, V. Chaturvedi, M. Ghannoum, K. C. ...
Jul 5, 2006 - dermatophytes (n=5) and Penicillium marneffei (n=4)). Yeasts. Accepted : 03-03-06 were grown on Sabouraud dextrose agar (HiMedia ...
27 Scedosporium prolificans, 24 Fusarium solani, and 13 Fusarium oxysporum isolates. The isolates were maintained as a suspension in sterile distilled water ...
psilopsis 47 (5.6%), Candida kefyr 38 (4.5%), Candida krusei 23 (2.7%), Candida dubliniensis 13 (1.5%) and C. intermedia, Candida lusitaniae and Candida ...
Remove the strips from the package, gripping the handle of the strip (area labelled E). Separate the ... Template for application. Use a template for optimal positioning of strips on the agar plate. .... Clinical Microbiology Newsletter , vol.22, no.
tometer (Bausch & Lomb, Pacisa, Madrid, Spain). All adjusted suspensions were quantified by plating on Sabouraud agar plates. The inocula were agitated for.
Mar 31, 2003 - vis-a`-vis Amphotericin B and Itraconazole. Rama Ramani,1 Madhurama Gangwar,1â and Vishnu Chaturvedi1,2*. Mycology Laboratory ...
JOHN H. REX,'* MICHAEL A. PFALLER,2 MICHAEL G. RINALDI,3. ANAMARIE .... mented techniques included broth dilution, agar dilution, and turbidimetric ...
Mar 29, 2018 - 2018 Khan et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at ...
Antifungal Susceptibility Testing for Dermatophytosis. When and How? (A Brief Review). 1Hari Pankaj Vanam, and 2P.Narsimha Rao. 1 Assistant Professor ...
on Clinical Laboratory Standards), has released four standard methods for
antifun- gal susceptibility testing including M27-A3 [ 1] for macrobroth and
microtiter ...
8/28/2012 jhindler CLSI M100-S22 Update. 1. 1. Update on the 2012-2013 CLSI
Standards for. Antimicrobial Susceptibility Testing: What's New with the.
of 13.7% of the isolates were found to be resistant, 47.1% were intermediate and 39.2% were sensitive to the drug. The rate of resistance of the yeasts against ...
Nov 1, 2013 - considerable differences in clinical susceptibility breakpoints between the ... breakpoints than CLSI, in particular for Gram-negative bacteria,.
azole, itraconazole and voriconazole) for the 108 Aspergillus spe- cies isolated from patients included in this study were determined by CLSI M38-A MD3 and ...
List of the various primers used for amplification and sequencing. ...... nog op morphologie en op fysiologische tests die dagen tot weken in beslag nemen en ...
Chapter 8. In vitro activities of eight antifungal drugs against 55 clinical isolates of ..... the growth in the wells containing the drug with the drug-free control. ...... PCR Protocols: A guide to methods and ...... 4.61 (Applied Maths, Kortrijk,
Copyright 0 1983, American Society for Microbiology. Vol. 23, No. 1. Comparison ... ed with 5% sheep blood (BBL Microbiology Systems,. Cockeysville, Md.) and ...
The author goes on to discuss susceptibility testing methodologies and present an approach to ... Organization (WHO) ini
Aug 23, 2012 - clinical breakpoints. ⢠Chosen by taking into account the MIC distribution of a species, the modal distribution, and the inherent variability in.
8/23/2012
ANTIFUNGAL SUSCEPTIBILITY TESTING METHODS • Macrobroth dilution • Microbroth dilution • Disk diffusion • Agar diffusion
CLSI UPDATES FOR ANTIFUNGAL SUSCEPTIBILITY TESTING ANNETTE FOTHERGILL*ASSOCIATE PROFESSOR * UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER
2
CLSI (NCCLS) PUBLICATIONS
M27-A3
• 2008 – M27-A3
• Inoculum
• Third edition
• 0.5-2.5 x 104 • 0.5 McFarland at 530 nm wavelength
• 2008 – M38-A2
• Incubation
• Second edition
• 24 hours
• 2004 – M44-A
• AMB, ANID, CAS, MICA, FLU, POSA, VORI
• Approved Standard
• 48 hours
• 2010 – M51-A
• AMB, FLU, 5FC, ITRA, POS, RAV, VORI
• 72 hours
• Approved Standard
• Cryptococcus sp. – all drugs 3
M27-A3 • Drug Preparation • AMB • DMSO
• Candins • DMSO (CAS and MICA previously water)
• Azoles • DMSO (FLU previously water)
• 5FC • DMSO (previously water)
M27-A3 • End Points • AMB • 100 % Inhibition
• Candins • 50% Inhibition (24 hours ONLY)
• Azoles • 50% Inhibition
• 5FC • 50% Inhibition
1
8/23/2012
EPIDEMIOLOGICAL CUTOFF VALUES • A value used to assist with interpretation of MIC data where sufficient clinical data is not available for the purpose of establishing clinical breakpoints • Chosen by taking into account the MIC distribution of a species, the modal distribution, and the inherent variability in MIC testing methods (typically 2 dilutions above the modal MIC)
EPIDEMIOLOGICAL CUTOFF VALUES • Although the exact percent varies per publication, most agree that the wild-type strains should comprise 95-99% of any test set • Ideally, test sets contain in excess of 1000 strains • Strains with MICs above the ECV are considered to have potentially acquired mechanisms of resistance
7
M27-A3 PROPOSED BREAKPOINTS CANDIDA ALBICANS
CURRENTLY PUBLISHED BREAKPOINTS FOR CANDIDA SPP. (µG/ML) 5FC FLU ITRA VORI ANID CAS MICA
S 2
**There are no breakpoints for Cryptococcus spp., Trichosporon spp., Saccharomyces spp., etc.
