appendix appendix table s1 detailed statistical results figure 2b figure

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Figure 2B. Figure 3B. Figure 4B. N value exact p value asterics. N value exact p value asterics. N value exact p value asterics. UNT. 11. N/A. UNT. 8. N/A. UNT. 5.
APPENDIX APPENDIX TABLE S1 DETAILED STATISTICAL RESULTS FIGURE 2B FIGURE 2D FIGURE 2E FIGURE 2F FIGURE 3B FIGURE 3C FIGURE 3F FIGURE 3G FIGURE 4B FIGURE 4C FIGURE 4D FIGURE 4E EXPANDED FIGURE EV1D

Figure 2B

Figure 3B UNT Black Yellow GFP-+ GFP++

N value 11 5 5 12 12

exact p value N/A 0.4988 6.11362E-06 0.027629843 3.12849E-06

ns **** * ****

2-3 days

UNT GFP-+ GFP++

N value 8 8 8

exact p value N/A 0.2533 0.0002

15-20 days

UNT GFP-+ GFP++

7 7 7

N/A 0.0227 1.99367E-05

UNT GFP-+

6 6

N/A 0.0269

*

GFP++

6

0.0002

***

Figure 2D

1 day

15 days

N value 3 3

exact p value N/A 0.0145

asterics

UNT GFP-+ GFP++

3

0.0016

**

UNT GFP-+ GFP++

3 3 3

N/A 0.2317 0.006

*

23-27 days

1 day

15 days

N value 6 5 6

exact p value N/A 0.852 0.0013

UNT GFP-+ GFP++

4 3 3

N/A 0.5998 0.0242

asterics UNT GFP-+ GFP++ WT UNT GFP-+ GFP++ WT UNT GFP-+

N value 5 5 5 4 6 5 5 4 6 5

exact p value N/A 0.0021 0.0268 0.0005 N/A 0.0011 0.0321 0.0011 N/A 0.0012

GFP++

5

0.0494

*

WT UNT GFP-+ GFP++ WT

4 6 5 5 4

0.0055 N/A 0.0006 0.0178 0.0002

**

N value 6 5 5 4

exact p value N/A 0.0003 0.0002 0.0002

asterics

UNT GFP-+ GFP++ WT

N value 4 5 5 2

exact p value N/A 0.0589 0.0026 N/A

asterics

UNT GFP-+ GFP++ WT

N value 6 6 6 6 6 6 6 6 6

exact p value N/A 0.075 0.0315 N/A 0.0211 0.1533 N/A 0.4805 0.5787

asterics

UNT GFP-+ GFP++ UNT GFP-+ GFP++ UNT GFP-+ GFP++

1 day

N value 10 9 9

exact p value N/A 0.5635 0.0023

asterics

UNT GFP-+ GFP++

15 days

UNT GFP-+ GFP++

4 4 3

N/A 0.7572 9.00458E-05

ns ***

Basal

* ****

Maximal

Spare capacity

Figure 3C ns ** 2-3 days

UNT GFP-+ GFP++

N value 7 7 7

exact p value N/A 0.131 8.96389E-05

15-20 days

UNT GFP-+ GFP++

5 6 6

N/A 0.9012 0.00051

UNT GFP-+ GFP++

6 6 6

N/A 0.382 0.00251

Figure 2E UNT GFP-+ GFP++

Figure 4B

asterics

asterics

ATP-linked

ns ****

asterics ** * *** ** * ** **

*** * ***

asterics ns **

ns *

23-27 days

Figure 4C ns *** Coupling efficiency (%) ns **

*** *** ***

Figure 4D Figure 2F

1 day

UNT GFP-+ GFP++

N value 6 6 6

exact p value N/A 0.1692 0.3796

asterics ns ns

Figure 3F

2 day

15 days

N value 4 4 4 4 3 3 3 3

exact p value N/A 0.0036 0.0145 0.0001 N/A 0.7704 0.0087 3.2429E-05

asterics

UNT GFP-+ GFP++ GFP+++ UNT GFP-+ GFP++ GFP+++

** ****

NDUFB8

N value 7 7 7 4 4 4 4 4

exact p value N/A 0.2742 0.00006 3.74249E-12 N/A 0.9277 0.0907 0.5048

asterics

COXI

UNT GFP-+ GFP++ GFP+++ UNT GFP-+ GFP++ GFP+++

ns **** ****

SDHA

Mitochondrial Protein synthesis

** * *** Figure 4E

Figure 3G

2 day

15 days

ns **

ns ns ns

ns * ns * ns ns ns ns

Fig. EV1D

ns **

ns ****