ZMBH Alliance), Germany; 2Institute of Pathology, Heidelberg University, Germany; 3Institute of. Pathology, Technical University Munich, Germany; 4Department ...
Appendix Hepatic stellate cells limit hepatocellular carcinoma progression through the orphan receptor endosialin Carolin Mogler,1,2,3*, Courtney König1,4*, Matthias Wieland1,4, Anja Runge1,4, Eva Besemfelder1, Dorde Komljenovic5, Thomas Longerich6, Peter Schirmacher2, and Hellmut G. Augustin1,4,7
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Division of Vascular Oncology and Metastasis, German Cancer Research Center Heidelberg (DKFZ-
ZMBH Alliance), Germany; 2Institute of Pathology, Heidelberg University, Germany; 3Institute of Pathology, Technical University Munich, Germany; 4Department of Vascular Biology and Tumor Angiogenesis (CBTM), Medical Faculty Mannheim, Heidelberg University, Germany; 5Department of Medical Physics in Radiology, German Cancer Research Center Heidelberg, Germany; 6Institute of Pathology, RWTH Aachen, Germany; 7German Cancer Consortium, Heidelberg Germany.
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Table of contents Appendix Figure S1: Endosialin expression on human dysplastic nodules (whole tissue slides). Appendix Figure S2: Heterogeneous Endosialin expression on HCC tissue microarray punches. Appendix Figure S3: Co-stainings of Endosialin and CD31 on human HCC. Appendix Figure S4: Immunohistochemical analysis of non-tumorigenic WT and Endosialindeficient livers. Appendix Figure S5: Computer tomographical analysis of tumorigenic WT and Endosialindeficient mice. Appendix Figure S6: Expression profiling and functional analysis of conditioned media from nsEN and shEN HSC. Appendix Table S1: Complete list of cytokines analyzed in the conditioned media of shEN and nsEN HSC.
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Appendix Figure S1
Appendix Figure S1. Endosialin expression on human dysplastic nodules (whole tissue slides). Immunohistochemical staining for Endosialin on whole tissues slides of Dysplastic nodules (200x magnification). Scale bar: as indicated.
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Appendix Figure S2
Peritumorous liver
HCC
HCC
Appendix Figure S2. Heterogeneous Endosialin expression on HCC tissue microarray punches. Immunohistochemical stainings of Endosialin on 57 HCC and peritumorous liver parenchyma tissue microarray punches. Scale bars: as indicated. HCC=hepatocellular carcinoma.
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Appendix Figure S3
Appendix Figure S3. Co-stainings of Endosialin and CD31 on human HCC. Immunohistochemical double staining of Endosialin (brown) and CD31 (red) of different HCC samples (n=8). Scale bars: as indicated.
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Appendix Figure S4
Appendix Figure S4. Immunohistochemical analysis of non-tumorigenic WT and Endosialin-deficient livers. HE stainings of WT::iAST and ENKO::iAST livers in unchallenged settings do not display any overt phenotype. Scale bars: as indicated.
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Appendix Figure S5
Appendix Figure S5. Computer tomographical analysis of tumorigenic WT and Endosialin-deficient mice. Computer tomography of WT::iAST (A, C,) and ENKO::iAST (B,D) mice seven weeks after tumor induction as described in Figure 2. 2-dimensionally and 3-dimensionally reconstructed images. n= nodule; st=stomach; vc= vertebral cord.
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Appendix Figure S6
Appendix Figure S6. Expression profiling and functional analysis of conditioned media from nsEN and shEN HSC. Cytokine array of CM from nsEN and shEN HSC highlighting the top 5 differentially expressed cytokines (A). EdU proliferation assay of Huh7 tumor cells stimulated with the top 2 cytokine candidates: CCl5 (B) or RBP4 (C). n=1 with 3 technical replicates. Data are expressed as mean ± SD. Statistical analysis: Student´s t-test.
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Appendix Table S1. List of cytokines. Human cytokine array from the conditioned media of shEN and nsEN HSC.
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