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Application of standard addition to eliminate ... - Springer Link
Mossoba, M.M., R.E. McDonald, J.A.G. Roach, D.D. Fingerhut,. M.P. Yurawecz, and N. Sehat, Spectral Confirmation of Trans Mo- nounsaturated C18 Fatty Acid ...
Application of Standard Addition to Eliminate Conjugated Linoleic Acid and Other Interferences in the Determination of Total Trans Fatty Acids in Selected Food Products by Infrared Spectroscopy M.M. Mossobaa,*, J.K.G. Kramerb, J. Fritschea, M.P. Yuraweczc, K.D. Eulitzc, Y. Kuc, and J.I. Raderc U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, aOffice of Scientific Analysis and Support and cOffice of Nutritional Products, Labeling, and Dietary Supplements, Washington, DC 20204, and bFood Research Program, Agriculture and Agri-Food Canada, Guelph, Ontario, Canada N1G 5C9
ABSTRACT: A novel and rapid (5 min) attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopic method AOCS Cd 14d-99 for the determination of total isolated trans fatty acids, which absorb at 966 cm−1, was recently developed, collaboratively studied, and applied to food products containing 1–50% trans fat (as percentage of total fat). Attempts to apply the ATR-FTIR method to biological matrices of low trans fat and/or low total fat content, and to dairy and other products were not satisfactory due to interfering IR absorptions in the trans region. One group of interfering compounds with absorption bands near 985 and 948 cm−1 was the cis/trans positional isomers of conjugated linoleic acid (CLA) found in dairy and meat products from ruminants at levels of
