Application of Stochastic Labeling with Random-Sequence ... - PLOS
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Application of Stochastic Labeling with Random-Sequence ... - PLOS
Mar 3, 2017 - PLoS ONE 12(1): e0169431. doi:10.1371/journal.pone.0169431 PMID: 28052139. PLOS ONE | DOI:10.1371/journal.pone.0173546 March 3, ...
CORRECTION
Correction: Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes Tatsuhiko Hoshino, Fumio Inagaki
There are errors in the fourth and fifth sentences of the Quantification by qSeq subsection of the Materials and Methods section. The correct sentences are: The SPE conditions consisted of initial denaturation at 98˚C for 2 min, cooling to 55˚C at 0.3˚C/s, and elongation at 68˚C for 10 min. Subsequently, excess primers were digested by addition of 4 μl of Exonuclease I (5 U/ μl, TaKaRa Bio) and incubation at 37˚C for 120 min, followed by inactivation of the enzyme by incubation at 80˚C for 30 min.
Reference 1.
Hoshino T, Inagaki F (2017) Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes. PLoS ONE 12(1): e0169431. doi:10.1371/journal.pone.0169431 PMID: 28052139