Assessment of Chlorine & Non Chlorine Oxidizing Agent against HP Avian Influenza virus. A. M. Gamal1, M. M. Hamoud2, A.M. Metwally3, Elshaimaa Ismael1, ...
Assessment of Chlorine & Non Chlorine Oxidizing Agent against HP Avian Influenza virus A. M. Gamal1, M. M. Hamoud2, A.M. Metwally3, Elshaimaa Ismael1, M.A. Rahim 3, Shimaa A.E. Nasr1 S. T. Moubarak1, Manal M. Zaki1, M. M. Ali1, H.A. Hussein3, O.K Zahran1 1
Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt 2 Department of Poultry disease and management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt 3 Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt SUMMARY. Inactivation of AIV on the surfaces of objects or in the air at poultry farms would significantly reduce and or limit the chance for its circulation and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is still a need for their evaluation under different conditions and in different ways. Four chemical disinfectants were evaluated for their virucidal ability against two H5N1 HPAIVs AIV). The first virus A/chicken/Egypt/VRLCU67/ 2011) variant subclade 2.2.1.1, while the second virus (A/chicken/Egypt/13VIR3729-4/2013) classic subclade 2.2.1/C. The purpose from using local Egyptian H5N1viruses in the evaluation was to achieve maximum simulation of Egyptian field reality as the two viruses represents the two main sublineages (classic 2.2.1/c and variant 2.2.1.1.) currently circulating in Egypt. The used disinfectants were 1% virkon S©, 325 ppm, calcium hypochlorite (bleaching powder) (65%), 250 ppm Sodium hypochlorite (5%) and Peraclean 5%®. The disinfectants were tested individually for effectiveness against high pathogenic avian influenza virus for 5, 10, 15 and 30 minutes’ contact time. A numerical method and neutralization test were used to express the ability of each disinfectant agent to inactivate virus. Each one of the previously mentioned disinfectants can be effective against avian influenza virus when we increase the contact time more than 15 minutes except in Virkon -s which was effective even at short contact time 5 minutes. We could conclude that chlorine & non chlorine oxidizing agent can be used to inactivate AIV at the farm level. Key words: HPAI virus; virkon- S©, calcium hypochlorite; Sodium hypochlorite; Peraclean; poultry; Egypt
