Proceedings of the XLIX Italian Society of Agricultural Genetics Annual Congress Potenza, Italy – 12/15 September, 2005 ISBN 88-900622-6-6 Poster Abstract - C.01
ASSESSMENT OF GENETIC DIVERSITY OF EUROPEAN EMMER WHEAT POPULATIONS BY EST-SSR, ISSR AND SSR MOLECULAR MARKERS L. MONDINI*, M. A. PAGNOTTA*, H. GRAUSGRUBER**, E. PORCEDDU* *) Department of Agrobiology and Agrochemistry - Tuscia University, Via S. C. de Lellis 01100 Viterbo, Italy -
[email protected] **) BOKU - University of Natural Resources and Applied Life Sciences, Department of Applied Plant Sciences and Plant Biotechnology, Institute of Agronomy and Plant Breeding, Gregor Mendel Str. 33, A1180 Vienna, Austria
Triticum dicoccum, EST-SSR, ISSR, SSR, variability Emmer wheat has been rediscovered in the last decades due to its characteristics. Moreover, its wild behaviour made its cultivation in the marginal lands interesting from the economical point of view. A total of 38 emmer wheat (Triticum dicoccum) accessions, collected around Europe from: Germany (15 accessions), Austria, Italy (8 accessions), Spain (3 accessions), Slovakia (2 accessions), and Israel, were evaluated utilizing both agro-morphological characteristics and molecular markers. The agronomic traits evaluated were: vernalisation response, winter hardiness, date of heading and flowering, lodging, plant height at harvest, and resistances against powdery mildew (Erysiphe graminis), leaf rust (Puccinia recondita), and yellow rust (Puccinia striiformis). Quality control was also performed measuring the protein content, gluten quality and quantity, and a baking test was performed. The molecular evaluation was carried out utilizing 12 microsatellite (EST-SSR and SSR) and 6 ISSR primers for a total of 107 loci analysed. Some of the EST-SSR markers have homology to genes of known function such as: ABA induced protein, PM-19, alcohol dehydrogenase, ADP/ATP translocator gene, and CERI/like 3' gene. Mean 1000 kernel weight ranges from 31.6 to 39.0 g for winter emmer accessions and from 22.9 to 42.6 for spring emmer accessions. Almost all spring emmer accessions showed resistance to powdery mildew. The protein content of both winter and spring emmer was significantly effected by environment and genotype (P
