May 25, 2010 - genes if they conform to the 'two-hits' or. âmultiple hitsâ hypothesis which postulates that ..... [37] Knudson AG. Hereditary cancer: two hits revis-.
Int J Clin Exp Pathol 2010;3(5):482-491 www.ijcep.com /IJCEP1004010
Original Article Association of the promoter methylation and protein expression of Fragile Histidine Triad (FHIT) gene with the progression of differentiated thyroid carcinoma De-Tao Yin1,4, Lin Wang2, Jianrui Sun3, Fengyan Yin1, Qingtao Yan4, Rulong Shen4, Gang He4, Jian-Xin Gao4 1Department
of General Surgery, 2Department of Information, and 3Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, P. R. China; 4Department of Pathology and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA. Received April 27, 2010, accepted May 18, 2010, available online: May 25, 2010 Abstract: The role of aberrant methylation of fragile histidine triad (FHIT) promoters in the differentiated thyroid carcinoma (DTC) is not yet clear. Therefore, we investigated the association of the status of FHIT promoter methylation and FHIT protein expression with the clinicopathological progression of DTC, using PCR-based methylation assay and immunohistochemical technique. While no FHIT gene promoter methylation was observed in the matched noncancerous epithelium (NCE) specimens, 24.6% of DTC samples demonstrated methylation in the FHIT promoter region. The protein expression of FHIT in NCE and DTC was 100.0% and 41.5% (P
