After washing, samples were incubated in primary antibodies, followed by secondary antibodies with DAPI for nuclear staining. Primary antibodies used were ...
Stem Cell Reports, Volume 8
Supplemental Information
Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells David J. Tischfield, Junho Kim, and Stewart A. Anderson
Supplemental Figure S1. aPKCi increases the proportion of Ccnd2-expressing Nkx2.1 progenitors in two additional mESC lines, Related to Figure 1. (A). Quantification of the proportion of Nkx2.1 progenitors that co-express Ccnd2 at DD11. J1 (ATCC® SCRC-1010™) is the parent line of JQ27, used in this study. JQ59 is another J14 (Maroof et al 2010) subclone that also contains the Nkx2.1::mCherry BAC. The mCherry reporter expression in this line, however, is minimal. R1/E (ATCC® SCRC1036™) is a separate stem cell line that was differentiated using the protocol described in this study. *p
