Automated Segmentation and Classification of ... - Stanford InfoLab

$####""#!" "#( "!!$$# #(

D;3@3@363 7AD9;3*:A?3E "7;F::7@93@6!3?7E/ -3@9 @F79D3F;H7;AE5;7@57E'DA9D3? 7B3DF?7@FA87>>$A>75G>3D;A>A9K 7B3DF?7@FA8'3F:A>A9KA>>797A8 @8AD?3F;A@)5;7@57E3@6*75:@A>A9K *:7'7@@EK>H3@;3)F3F7+@;H7DE;FK

*$" #" " ") ! %# # !% )!" " !" ! "#$( $## #(" $" +#!$$#, "# (""#$#!!#"$" $##)# )!" !% "#( "" $# ! # "! ## # !"$# %!#$ "" " !( $ #!! !# # & #%#" # %# "("#" ! $## ! #!)# "#( " !"# !##( ! $## "## ""# "#( " " &# # "#$( $" !% )! "($#""&###"### " # !" %$ (!" $# &# !" $" #" &($" !"" !#" ## # !"$# ""# "("# " " #!!# '#" !" !!

%*(&+* &%

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

;9 GDD7@F>34AD3FADKB;B7>;@78ADN:;9:F:DAG9:BGFO:;EFA>A9K *:7 EF7BEGBFA3@6;@5>G6;@96;9;F;L3F;A@53@473GFA?3F763@6 AD5A@6G5F76 ;@ B3D3>>7> 4GF F:7 AH7D3>> BDA57EE ;E D3F7>;?;F76 4K F:7 ?3@G3> 3@6 F76;AGEBDA57EEA8E5AD;@93@63@@AF3F;A@

,))%+&)+*"%0)"*!"$ %#/*"*

'D7E7@F>KF:7A@>K?3KE;E:3E5A?78DA?-A@9 3@65A>>739G7EI:A67H7>AB763?7F:A6018ADCG3@F;F3F;H7 @7GDA@3> B:7@AFKB;@9 43E76 A@ 3GFA?3F;5 3@3>KE;E A8 >;9:F ?;5DAE5AB7 ;?397E A8 >;H7 L74D38;E: 7?4DKAE I;F: 3>F7D76 7JBD7EE;A@ A8 >L:7;?7DPE 6;E73E7 >;@=76 97@7E @ B3DF;5G>3DF:;E?7F:A63>>AIE8ADCG3@F;F3F;H73EE7EE?7@FA8 @7GDA@ >AEE 3GFA?3F76 67F75F;A@ A8 EA?;F7E 9DAGBE A8 ?7EA67D?57>>EF:3F8AD?3>A@9F:7@7GD3>FG473@69AA@FA 6;887D7@F;3F7 ;@FA E=;@ ?GE5>7 3@6 H7DF74D37 3@6 67F7D?; @3F;A@A897@77JBD7EE;A@>7H7>E;@F:738875F767?4DKAE @ BD;@5;B>7 3GFA?3F76 ?7F:A6E EG5: 3E F:;E 53@ 47 D736;>K E53>34>7FA>3D97E53>7B:7@AFKB;@9EFG6;7E;@I:;5:?3@G3> ;?3973@3>KE;E;E;@788;5;7@F3@6H3D;34>701 *AAGD=@AI> 7697 :AI7H7D @A 5A?BGF7D;L76 ?7F:A6E 3D7 3H3;>34>7 8AD F:7 3GFA?3F76 CG3@F;F3F;H7 3@3>KE;E A8 ;?397E A8 L74D38;E:

245 c 2008 IEEE 978-1-4244-1813-8/08/$25.00

L3F;A@A83@7@F;D73DD3KA8>3DH37?3K475A@6G5F76;@B3D 3>>7>;@F:7E7@E7F:3F;@735:A8F:7E7EF7BE?G>F;B>7>3DH37 3D7 47;@9 BDA57EE76 FA97F:7D F:7 BDA57EE A8 E5AD;@9 735: ;?397 ;E D3F7 >;?;F;@9 A?BD7:7@E;H7 3@@AF3F;A@ A8 F:7 B:7@AFKB7D7CG;D7EE5AD;@9E7H7D3>>7H7>EB>3@3DE75F;A@EA8 735:>3DH3 -:;>7F:;EBDA57EE;E?3@39734>7I:7@?3@G3>>K 3@@AF3F;@9 3 E?3>> @G?47D A8 >3DH37 F:7 F3E= 475A?7E ;@ 5D73E;@9>K;?BD35F;53>3EF:7@G?47DA8>3DH37D;E7E A?B>; 53F;@9F:7?3FF7D;EF:7BDA4>7?A8EG4A9K ;?397E -:;>7 >3D97>K D7>;34>7 3@6 GE78G> 5>;@;53>>K F:7 CG3>;F3F;H7 3EB75FE A8 5GD D7@F :;EFA>A9;53> 3EE7EE?7@FE D7EG>F ;@ ;@FD3 3@6 ;@F7D A4E7DH7D H3D;34;>;FK 01 *:;E H3D;34;>;FK 53@ 47 6G7 FA 6;8 87D7@57E ;@ FD3;@;@9 34;>;FK F;?;@9 3@6 7JB7D;7@57 *:7D7 8AD7 ;@ AD67D FA ?3J;?;L7 F:7 78875F;H7@7EE A8 :;EFA>A9;53> EFG6;7E3E3BB>;76FA3@;?3>?A67>EKEF7?E;F;E5D;F;53>F:3F EA?7 8AD? A8 3GFA?3F;5 CG3@F;F3F;H7 ?7F:A6 47 67H7>AB76 8ADF:73@3>KE;EA8:;EFA>A9K;?397E

!"*+&#& / *A 366D7EE F:;E 678;5;7@5K I7 3D7 67H7>AB;@9 ) (/ )KEF7? A8 ;EFA>A9;53> ?397 (7FD;7H3> 3@6 @ @AF3F;A@ 8AD /AAB3F:A>A9K 3 @AH7> 5A?BGF3F;A@3> 8D3?7 IAD= 8AD F:7 3GFA?3F76 A@FA>A9K43E76 3@@AF3F;A@ 3@6 D7FD;7H3> A8 :;EFA>A9;53> ;?397E 8AD CG3@F;F3F;H7 N:;9: F:DAG9:BGFO B:7@AFKB;@9 ;@ 3@;?3> ?A67> EKEF7?E *:;E 8D3?7IAD= ;E G>F;?3F7>K ;@F7@676 8AD GE7 I;F: :;EFA>A9K ;?397E 8AD 3 H3D;7FK A8 3@;?3> ?A67>E 4GF AI;@9 FA AGD 7JB7D;7@57 ;@ L74D38;E: 97@7F;5 EFG6;7E 3@6 :;EFA>A9;53> 3@3>KE;E L74D38;E: E7DH7E 3E 3 5A?B7>>;@9 B;>AF ?A67> 8AD BDAFAFKB7 67H7>AB?7@F 7D7 I7 BD7E7@F AGD BD7>;?;@3DK IAD=A@F:7E79?7@F3F;A@873FGD77JFD35F;A@3@65>3EE;8;53 F;A@ ?7F:A6E F:3F I;>> 8AD? F:7 8AG@63F;A@ A8 F:7 ) (/ 8D3?7IAD=

3D7F:7@E79?7@F768DA?F:73D734AG@64KF:7 '#3@6F:7 ('3EEG?;@9F:7 '#7J;EFEI:;5:;E@AFF:753E7;@?3@K E7H7D7>K 6;EAD93@;L76 ?GF3@FE &@57 F:7 '(# 3@6 %# 3D7 8AG@6 F:7 # ;E ;EA>3F76 8DA? F:7 D7?3;@;@9 NG@E79?7@F76O3D73A8F:77K7 FE:AG>647@AF76F:3F3FF:7 >AID7EA>GF;A@3@6D76G5765A>ADEB357GE76;@F:7BDAFAFKB7 3BB>;53F;A@ F:7 &'# ;E GEG3>>K FAA F:;@ FA 47 67F75F76 I;F: 3@K679D77A8DA4GEF@7EE3@6EA;FD7?3;@EG@E79?7@F763E B3DFA87;F:7DF:7'(#AD %# ;9 E:AIE3@7J3?B>7A83 ?GF3@F 7K7 E79?7@F76 4K F:7 ) (/ BDAFAFKB7 ;@FA ;FE 5A@EF;FG7@F57>>>3K7DE

$*&) @AD67DFA67?A@EFD3F7BDAA8A8BD;@5;B>7I7:3H767H7> AB76 3 IAD=;@9 BDAFAFKB7 GE;@9 F:7 F75:@;53> 5A?BGF;@9 >3@9G397 $*# *:7 E79?7@F3F;A@ 3@6 873FGD7 7JFD35 F;A@ 3>9AD;F:?E 3E I7>> 3E 5>3EE;8;53F;A@ ?A67>E:3H7 477@ 67H7>AB76FA3@3>KL7;?397EA8>3DH3>L74D38;E:7K7D7F;@3 3@69GF;@F7EF;@7 -7:3H75:AE7@FA;@;F;3>>K7J3?;@77K7 3@6 9GF 4753GE7 F:7E7 F;EEG7E :3H7 3@ ;@:7D7@F BA>3D;FK 3@6 N6;D75F;A@3>O AD93@;L3F;A@ F:3F I:7@ 6;EDGBF76 D7EG>FE ;@ ?GF3@FB:7@AFKB7EF:3F3D7D7>3F;H7>K73EK8AD:G?3@7JB7DFE FA67F75F #"')')+"&%%"$ ')')&**"% /74D38;E: >3DH37 I7D7 5A>>75F76 8;J76 3@6 7?476676 3E 67E5D;476 ;@ 0 1 K7 3@6 9GF ;?397E I7D7 ?3@G3>>K 5DABB76 8DA? J H;DFG3> E>;67E A8 :7?3FAJK>;@ 7AE;@ EF3;@76 L74D38;E: E75F;A@E 53BFGD76 GE;@9 3@ B7D;A * E>;67 E53@@7D ;@ F:7 '7@@ )F3F7 /74D38;E: G@5F;A@3> 7@A?;5E35;>;FK @AD67DFAD76G57F:75A?BGF3F;A@3>5AEF A8;?397E79?7@F3F;A@3@6873FGD77JFD35F;A@F:7;?397EA8 F:7 L74D38;E: 7K7 3@6 9GF GE76 ;@ AGD BDAFAFKB7 I7D7 5A@ H7DF76FA9D3KE53>75A>ADEB3573@68;@3>>KE53>766AI@FA3 ECG3D7 ?3FD;J 85DABB;@9AGFF:73D73A8;@F7D7EF 5AG>6@AFK;7>63ECG3D7?3FD;JF:7;?397I3EB36676I;F: I:;F7B;J7>EFA8AD?3 ?3FD;J

/* $%++"&%%+,).+)+"&% *:7 L74D38;E: 7K7 5A@E;EFE A8 E7H7D3> 6;EF;@5F >3K7DE ;@ 5>G6;@9 F:7 >7@E F:7 93@9>;A@ 57>> >3K7D # F:7 ;@@7D B>7J;8AD?>3F7D '#F:7;@@7D@G5>73D>3K7D %#F:7AGF7D B>7J;8AD? >3K7D &'# F:7 B:AFAD757BFAD >3K7D '(# 3@6 F:7 D7F;@3> B;9?7@F76 7B;F:7>;G? (' 0 1 *:7 E79?7@F3 F;A@ A8 F:7 L74D38;E: >3DH3> 7K7 ;E 53DD;76 AGF 4K3 E7D;7E A8 ;?397 BDA57EE;@9 AB7D3F;A@E ;@5>G6;@9 :;EFA9D3? 7CG3>;L3 F;A@ 5A@@75F76 5A?BA@7@F 3@3>KE;E F:D7E:A>6;@9 7697 67F75F;A@ 3E I7>> 3E E7H7D3> ?ADB:A>A9;53> AB7D3F;A@E EG5: 3E 6;>3F;A@ 7DAE;A@ 5>AE;@9 3@6 AB7@;@9 *:7 B3D3?7F7DE GE76 8AD 735: A8 F:7E7 AB7D3F;A@E I7D7 :7GD;EF;53>>K 67F7D ?;@764GF3D73>AAE78G@5F;A@A8F:7E=7I@7EEA8F:7;?397 :;EFA9D3? 35:57>>>3K7D;@F:7L74D38;E:7K7GE7E36;887D 7@F 5A?4;@3F;A@ A8 F:7E7 ;?397 BDA57EE;@9 AB7D3F;A@E FA 35:;7H7 F:7 ?AEF DA4GEF 67F75F;A@ A8 >3K7D 4AG@63D;7E *:7 >3K7DE I;F: F:7 ?AEF 6;EF;@5F 4AG@63D;7E M F:7 >7@E F:7 ('3@6F:7 '#M3D767F75F768;DEF *:7'(#3@6F:7 %#

246

;9 (7EG>FE A8 3GFA?3F;5 E79?7@F3F;A@ 4K ) (/ A8 3 ?GF3@F L74D38;E: 7K7 ;?397 ;@FA ;@6;H;6G3> 57>> >3K7DEI;F: 3@ 7JB>A676 H;7I A@ F:7 D;9:F *:7 ?GF3@F B:7@AFKB7 A8 F:7 6;EDGBF76 ;@@7D B>7J;8AD? >3K7D '#E77GBB7DD;9:FB3@7>;E5>73D>K67F75F76

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

2007 IEEE/NIH Life Science Sysytems and Applications Workshop (LISSA 2007)

%"+/ *:7E7F7JFGD7873FGD7E3D75A?BGF768DA?364>A5=E;@E5D;476I;F:;@735:E79?7@F7657>>>3K7D

,+* $%++"&%%+,).+)+"&% *:7 L74D38;E: >3DH3> 9GF ;E 5:3D35F7D;L76 4K E7H7D3> 873 FGD7E ;@5>G6;@9 F:7 F:;5=@7EE 3@6 679D77 A8 8A>6;@9 A8 F:7 7B;F:7>;3> >;@;@9 F:7 BA>3D;FK A8 F:7 7B;F:7>;3> 57>>E "F:7 BAE;F;A@ A8 F:7 @G5>7; I;F: D7EB75F FA F:7 43E7?7@F ?7? 4D3@7A8F:77B;F:7>;G?F:7679D77A88A>6;@9A8F:7>G?7@ I:;5:?3K475:3D35F7D;L764KF:7@G?47DA86;EF;@5FH;>>; BDAFDG6;@9 ;@FA F:7 >G?7@ 3E I7>> 3E F:7 3?AG@F 3@6 N9D3@G>3D;FKO A8 57>>G>3D 674D;E 3@6 ?G5AGE F:3F :3E E>AG9:76A88A8F:7>;@;@9;@FAF:7>G?7@ *:7E79?7@F3F;A@ BDA576GD7 479;@E I;F: F:7 67F75F;A@ A8 F:7 >G?7@ 8A>>AI76 4K3@3BBDAJ;?3F7E79?7@F3F;A@A8F:77B;F:7>;3>>;@;@9F:7@ ;67@F;8;53F;A@A8F:74AG@63D;7EA87B;F:7>;3>57>>@G5>7;3@6 F:7679D77FAI:;5:F:77B;F:7>;3>57>>E:3H7477@BA>3D;L76 3E I7>> 3E F:7 67F75F;A@ A8 5;>;3 3@6 3@K E>AG9:;@9 A88 A8 674D;E 3@6 ?G5AGE ;@FA F:7 >G?7@ *:7 873FGD7 7JFD35F;A@ BDA576GD78AD9GF;?397E;EE;?;>3DFAF:3FGE768ADF:77K7E 4GF ;@ AGD BDAFAFKB7 A@>K 873FGD7E 3D7 7JFD35F76 8AD 735: 9GF;?3973EABBAE76FA 873FGD7E8ADF:77K7;?397E *:7 :;9:7D@G?47DA87K7;?397873FGD7ED7EG>FE?3;@>K8DA?F:7 835F F:3F E;?;>3D E7FE A8 873FGD7E 3D7 7JFD35F76 8AD 735: E79 ?7@F76 57>> >3K7D 3@6 F:7D7 3D7 3 9D73F7D @G?47D A8 EG5: >3K7DE;@F:77K7;?397EF:3@;@F:79GF;?397E #**""+"&% *:7 5GDD7@F H7DE;A@ A8 F:7 ) (/ BDAFAFKB7 GE7E 3 FD7743E76 3>9AD;F:? EB75;8;53>>K F:7 >3EE;8;53F;A@ @6 (79D7EE;A@ *D77 AD N(*O 3>9AD;F:? 01 FA 5>3EE;8K F:7 7K73@69GF;?397E355AD6;@9FAF:7;D679D77A834@AD?3>;FK *:7 (* 3>9AD;F:? IAD=E 4K 8;@6;@9 3 :;7D3D5:K A8 873 FGD7EF:3FK;7>6F:747EF6;E5D;?;@3F;A@3?A@9F:767E;D76E7F A8 5>3EE7E F F:7 NDAAF @A67O A8 F:7 FD77 I:;5: D7BD7E7@FE F:7 8G>> 63F3 E7F F:7 47EF 679D77 A8 6;E5D;?;@3F;A@ AD N47EF EB>;FO ;E 5A?BGF76 8AD 3>> 873FGD7E &@57 3 47EF EB>;F :3E 477@ 5:AE7@ 8AD F:7 DAAF @A67 F:7 63F3 ;E B3DF;F;A@76 ;@FA FIAN5:;>6OADN>738O@A67E *:;EBDA57EE;ED7B73F76G@F;>3 BD75:AE7@ EFABB;@9 5D;F7D;A@ ;E D735:76 EG5: 3E I:7@ F:7 63F3:3E477@B3DF;F;A@767@AG9: EAF:3F3>>>738@A67E5A@ F3;@ 3F ?AEF 3 EB75;8;76 ?3J;?G? @G?47D A8 E3?B>7E 8F7DF:7FD77;E5A@EFDG5F76;F?3K478AG@6F:3FNBDG@;@9O F:7 FD77 435= FA 3 E?3>>7D @G?47D A8 F7D?;@3> @A67E ?3K ;?BDAH7F:7AH7D3>>5>3EE;8;53F;A@355GD35K -:;>7 ;F ?3K AD?3K @AF @757EE3D;>K K;7>6 F:7 47EF 355G D35K3E5A?B3D76FAAF:7D?7F:A6EEG5:3E3GEE;3@?;JFGD7 ?A67>EEGBBADFH75FAD?35:;@7E),$AD3DF;8;5;3>@7GD3> @7FIAD=E F:7 (* 3>9AD;F:? ;E 7EB75;3>>K GE78G> 4753GE7 ;FGE7E3NI:;F74AJO?A67>I:7D7F:7BDA57EEA85>3EE;8;53 F;A@53@47H;EG3>;L763E3FD77AD8>AI5:3DF3@6;EF:7D78AD7 73EK8AD?AEFB7AB>7FA5A?BD7:7@6E77;9 I:;5:53@ :7>BFABDAH;673E7@E7A8A4A9;53> 3EE7EE?7@F A8F7@ 3 :;9:>K EG4;FE 53@ A@>K 47 B7D8AD?76 A@ A@7 6;?7@E;A@ 3F 3 F;?7 AD AGD 3BB>;53 F;A@ :AI7H7D F:7 6;?7@E;A@3>;FK A8 F:7 63F3 ;E D7>3F;H7>K >AI 5A?B3D76 FA ?AD7 5A?B>7J 63F3E7FE F:3F ?3K 47 ?AD7 3?7@34>7 FA AF:7D 5>3EE;8;53F;A@ ?7F:A6E EG5: 3E EGBBADF

H75FAD ?35:;@7E I:;5: ?3=7 GE7 A8 >;@73D 3@6 @A@>;@73D 5A?4;@3F;A@E A8 873FGD7E 0 1 *:7 FKB7E A8 873FGD7E 5GD D7@F>KGE76;@AGDBDAFAFKB73BB>;53F;A@?3K@AF47B3DF;5G >3D>K EG;F76 FA @A@>;@73D 5A?4;@3F;A@E 4GF 7H7@ EA I7 3D7 B>3@@;@9 FA 7JB>AD7 ),$ 3@6 AF:7D 5>3EE;8;53F;A@ ?7F:A6E B7D:3BE ;@ 5A@6E F:7 47EF355GD35K

;9 @ 7J3?B>7 675;E;A@ FD77 478AD7 BDG@;@9 3E 97@7D3F76 4K F:7 (*3>9AD;F:?8AD5>3EE;8K;@97K7:;EFA>A9K;?397E

()+#*) *:7 ) (/ BDAFAFKB7 :3E 477@ F7EF76 8AD ;?397E A8 F:7 L74D38;E: >3DH3> 7K7 3@6 ;?397E A8 F:7 >3DH3> 9GF D3@9;@9;@3978DA? FA63KEBAEF87DF;>;L3F;A@6B8 @ 97@7D3> F:7 E79?7@F3F;A@E A8 4AF: F:7 7K7 3@6 9GF ;?397E BDA6G577J57>>7@FD7EG>FE7H7@8ADF:7?AEFE7H7D7?GF3@FE @EA?753E7E3DF;835FED7EG>F;@98DA?7DDADE;@F:7:;EFA>A9K E>;67 BD7B3D3F;A@ BDA57EE ?3K 38875F F:7 CG3>;FK A8 F:7 E79?7@F3F;A@ 4GF 5A@F;@G76 7@:3@57?7@FE FA F:7 E79?7@F3F;A@ 3>9AD;F:?E 3D7 ;@5D7?7@F3>>K ;?BDAH;@9 B7D8AD?3@57 @ 366;F;A@ F:7 E79?7@F3F;A@ 3>9AD;F:?E 3D7 CG;F7DA4GEFFA6;887D7@57E;@;?3974D;9:F@7EE5A@FD3EF3@6 E:3DB@7EE F:3F ?3K D7EG>F 8DA? H3D;3F;A@E ;@ F:7 ;?397 53BFGD7BDAFA5A> AI7H7DEA?7;?397E6ABD7E7@F?AD7A8 3 BDA4>7? F:3@ AF:7DE B3DF;5G>3D>K ;@ F:7 53E7 A8 67F75F;@9 F:7 4AG@63DK 47FI77@ F:7 '(# 3@6 %# >3K7DE I:;5: 4K H;DFG7 A8 F:7;D E;?;>3D;FK ;@ EF3;@;@9 5A>AD 3D7 EA?7I:3F 6;88;5G>F FA 6;E5D;?;@3F7 7EB;F7 F:;E A4EF35>7 F:7 355GD35K A8F:7'(# %#4AG@63DK67F75F;A@BDA576GD7MI:;5:@AI D7>;7E A@ F:7 6;887D7@57E ;@ F:7 E:3B7E 3@6 AD;7@F3F;A@E A8 735: >3K7DPE 57>>E 3E ABBAE76 FA 5A>AD 3@6 F7JFGD7M:3E ;?BDAH76 5A@E;67D34>K E;@57 67H7>AB?7@F 4793@ 3@6 5A@F;@G7EFA;?BDAH7 'D7>;?;@3DK 5>3EE;8;53F;A@ F7EFE I7D7 B7D8AD?76 GE;@9 F:D77?A67EA85>3EE;8;53F;A@;@5>G6;@9"%)/"5>3EE;8; 53F;A@3E@AD?3> I;>6FKB7HE 34@AD?3> ?GF3@F+!)#** @AD?3> ?A67D3F7>K 34@AD?3> AD E7H7D7>K 34@AD?3> 3@6 "-#**@AD?3>HE A@7A88AGDBAEE;4>7?GF3@F5>3EE7EA8 ;@5D73E;@9679D77EA834@AD?3>;FK 753GE7A8F:7D7>3F;H7>K E?3>>@G?47DA8;?397E3H3;>34>73FF:7F;?7I75:AE7@AF FA:3H7E7B3D3F763F3E7FE8ADFD3;@;@93@6F7EF;@9A8F:75>3E

2007 IEEE/NIH Life Science Systems and Applications Workshop (LISSA 2007)

247

E;8;53F;A@ ?A67> 3@6 I7 ;@EF736 GE76 F7@8A>6 3@6 >73H7A@7AGF 5DAEEH3>;63F;A@ >3EE;8;53F;A@ 355GD35;7E I7D75A?BGF763EF:7B7D57@F397A8F:7;?397E;@F:7F7EF;@9 B3DF;F;A@I:AE73GFA?3F;55>3EE;8;53F;A@?3F5:76F:79DAG@6 FDGF:5>3EE;8;53F;A@3E67F7D?;@764KBD;AD:G?3@7H3>G3F;A@ E E:AI@ ;@ *34>7 F:7 4;@3DK 5>3EE;8;53F;A@ B7D8AD?76 I7>> 8AD 4AF: 7K7 3@6 9GF ;?397E I;F: 355GD35;7E D3@9;@9 8DA?FA4GF3EF:7@G?47DA85>3EE7E;@5D73E7F:7 5>3EE;8;53F;A@ 355GD35K 5ADD7EBA@6;@9>K 675D73E7E B3DF;5G >3D>K8ADF:79GF;?397E *# )+$$(.&/( ) )*&#&. $#)) * &%()+#*) +) % &#% #,&%& +*(&)),# * &% ( "

$# " 8A>6 #73H7 8A>6 #73H7 """ , A@7AGF , A@7AGF ;@3DK *:D77 5>3EE7E ;H7 5>3EE7E

, )+)) &% *:7E7D7EG>FE3D7CG;F77@5AGD39;@93>F:AG9:?AD7EA8AD F:7 7K7 ;?397E 7EB75;3>>K ;@ >;9:F A8 F:7 835F F:3F 3>> AD 93@EB75;8;5;?397E:3H7477@9DAGB76FA97F:7D8AD5>3EE;8; 53F;A@ D793D6>7EE A8 F:7 397 A8 F:7 >3DH3 E:AI@ ;@ 3 9;H7@ ;?397 7@7D3>>K ;F ;E 6;88;5G>F FA 6;EF;@9G;E: :;EFA>A9;53> 34@AD?3>;F;7E;@KAG@97D6B8>3DH374753GE757>>>3K7D 6;887D7@F;3F;A@;EEF;>>BDA9D7EE;@93FF:;EEF397 *:;E?3K;@ B3DF7JB>3;@F:76;EB3D;FK47FI77@F:77K73@69GF7DDADD3F7E 8AD?G>F;5>3EE5:3D35F7D;L3F;A@ 673>>K;?397EA8>3DH37A8 6;887D7@F397EE:AG>6475>3EE;8;76E7B3D3F7>K4GF6GD;@9F:;E ;@;F;3> B:3E7 A8 ) (/ 67H7>AB?7@F @AF 7@AG9: @AD?3> 3@6?GF3@F;?397E8AD3@K9;H7@397I7D73H3;>34>7FAK;7>6 EFDA@9>K 5A@5>GE;H7 5>3EE;8;53F;A@ D7EG>FE E;@57 BD7BDA57EE ;@9;E5GDD7@F>K3F76;AGEBDA57EE A@E7CG7@F>K3>>397EA8 >3DH37 I7D7 5>3EE;8;76 FA97F:7D -7 3D7 ;@ F:7 BDA57EE A8 BD7B3D;@93?G5:>3D97DE7FA8;?397EEAF:3FI753@5A@6G5F ;@6;H;6G3> 5>3EE;8;53F;A@ F7EFE 8AD EB75;?7@E 3F EB75;8;5 397E 3>>;?397EA86B8>3DH3>7K7EI;>>475>3EE;8;76;@A@7 F7EF3>>6B8>3DH3>7K7EI;>>475>3EE;8;76;@3@AF:7DF7EF3@6 EA 8ADF: 3@6 3>EA EA F:3F I7 53@ :3H7 7@AG9: EB75;?7@E D7BD7E7@F3F;H7A8735:5>3EE"@AD?3>HE H3DK;@9679D77E A834@AD?3>;FK , &%#+) &%)%+*+(-&(" -7:3H767H7>AB763BDAFAFKB7EKEF7?8ADF:73GFA?3F76 E79?7@F3F;A@ 3@6 5>3EE;8;53F;A@ A8 L74D38;E: :;EFA>A9K ;? 397E I:;5: FA F:7 47EF A8 AGD =@AI>7697 ;E F:7 8;DEF EG5: BG4>;E:76IAD= AI7H7DF:;E;E?7D7>KF:7EF3DF;@9BA;@FA8 AGD D7E73D5: ;@ F:7 3GFA?3F76 5:3D35F7D;L3F;A@ A8 3@;?3> :;EFA>A9K 3@6 I:;>7 AGD BD7>;?;@3DK D7EG>FE 3D7 BDA?;E;@9 F:7BDAFAFKB7:3EE7H7D3>ABBADFG@;F;7E8AD;?BDAH7?7@FF:3F I7 3D7 5GDD7@F>K 366D7EE;@9 AD 7J3?B>7 ;?397E79?7@F3 F;A@;E@AF3>I3KE355GD3F7EG5:3EI;F:D7EB75FFAF:767F75 F;A@ A8 F:7 '(# %# 4AD67D ;@ F:7 7K7 ;?397E @ 366;F;A@

248

F:7 BDAFAFKB7 5GDD7@F>K GF;>;L7E >AID7EA>GF;A@ 9D3KE53>7 ;?397E ?3@G3>>K 7JFD35F76 8DA? F:7 AD;9;@3> 5A>AD H;DFG3> E>;67E -:;>7 F:;E ?3K 47 36H3@F397AGE 8DA? 3 5A?BGF3 F;A@3>5AEFEF3@6BA;@FI7?3K47;9@AD;@9;?BADF3@F;?397 67F3;>EEG5:3E873FGD7EA8;@6;H;6G3>57>>@G5>7;F:3F5AG>6 A@>K AF:7DI;E7 47 67F75F76 GE;@9 :;9:D7EA>GF;A@ 5A>AD ;? 397E -7 I;>> F:7D78AD7 67F7D?;@7 F:7 ABF;?G? 7JF7@F A8 BD7BDA57EE;@9 @757EE3DK FA ?3J;?;L7 E79?7@F3F;A@ 3@6 873FGD7 7JFD35F;A@ 355GD35K I:;>7 ?;@;?;L;@9 4AF: ?3@G3> 788ADF 3@6 5A?BGF3F;A@3> 5AEF *A F:;E 7@6 I7 3D7 67H7>AB ;@9 ?7F:A6E FA 3GFA?3F;53>>K 7JFD35F F:7 7K7E 3@6 9GF 8DA? F:7AD;9;@3>H;DFG3>E>;67EEAF:3F?3@G3>;?3975DABB;@9;E G@@757EE3DK >3EE;8;53F;A@ 355GD35;7E 3D7 7JB75F76 FA ;?BDAH7 3E F:7 E7F A8 3H3;>34>7 ;?397E 9DAIE FA ;@5>G67 ?AD7 D7BD7E7@F3 F;H7E A8 3>> 397E 3@6 5>3EE7E A8 :;EFA>A9;53> EB75;?7@E -7 3>EA D75A9@;L7 F:3F ;@ EB;F7 A8 F:7 GE78G>@7EE A8 3 NI:;F7 4AJO5>3EE;8;7DEG5:3EF:7(*3>9AD;F:?GE76:7D75>3E E;8;53F;A@ 355GD35;7E ?3K 3>EA 47 ;?BDAH76 4K F:7 GE7 A8 EGBBADFH75FAD?35:;@7E3GEE;3@?;JFGD7?A67>EAD3DF;8; 5;3> @7GD3> @7FIAD=E AD 3 5A?4;@3F;A@ A8 F:7E7 B7D:3BE ;@F79D3F76I;F:(*I:;5:I7I;>>7JB>AD7 -7B>3@FA8GDF:7D67H7>ABAGD) (/BDAFAFKB78DA?3 L74D38;E:;?397E79?7@F3F;A@3@65>3EE;8;53F;A@EKEF7?;@FA 3B3D3>>7>;L34>7A@FA>A9K43E768D3?7IAD=8AD3GFA?3F;53>>K 3@@AF3F;@9;?397EA83@;?3>:;EFA>A9K3EI7>>3EBDAH;6;@93 ?73@E 8AD D7FD;7H3> A8 ;?397E 3@6 CG3@F;F3F;H7 B:7@AFKB7 ;@8AD?3F;A@ 8DA? 3@ A@>;@7 63F343E7 )G5: 3 EKEF7? I;>> :7>B FA 4D;@9 F:7 E5;7@F;8;5 5A??G@;FK 5>AE7D FA FDG>K N:;9:F:DAG9:BGFO B:7@AFKB;@9 3@6 3>EA FA 3EE;9@ BAEE;4>7 8G@5F;A@E FA BD7H;AGE>K G@5:3D35F7D;L76 97@7E *:7 B:7@A FKB7;@8AD?3F;A@EFAD76;@F:763F343E7I;>>4743E76A@F:7 ':7@AFKB7 3@6 *D3;F &@FA>A9K III 4;AA@FA>A9K AD9 I;=; ;@67J B:B '*&$3;@2'397 FA 7@EGD7 ;@F7D5A?B3F;4;>;FK I;F: AF:7D A@FA>A9K43E76 4;A>A9;53> 63F3 D7BAE;FAD;7E 3@6 835;>;F3F7;@F7D35F;A@E3?A@93@;?3>?A67>D7E73D5:7DE ((%) 0 1 %GEE>7;@,A>:3D6 3@6 ( 3:? 76E )"*! )+"# '')&!&J8AD6&J8AD6+@;H7DE;FK'D7EE 0 1 ) *E3A-G -747D# ( G697A@3@6" :7@993DAE7 7?476676F;EEG73DD3KE8AD:;EFA>A9;53@697@7F;53@3>KE;E "&+! %"(,*HA> BB 01 % )343>;3GE=3E AGFL 7F 3> ;9:F:DAG9:BGF L74D38;E: :;EFA>A9K +!&*HA> BB 01 ' A>CG:AG@ ! ! %A9G7D3E 7F 3> @F7DA4E7DH7D 3@6 ;@FD3A4E7DH7D 4;3E 7J;EFE ;@ F:7 ;@F7DBD7F3F;A@ A8 3@3> 6KEB>3E;3 "*&#&%+,$ HA> BB 01 * #;G ! #G 7F 3> A?BGF7D;L76 ;?397 3@3>KE;E 8AD CG3@F;F3F;H7 @7GDA@3> B:7@AFKB;@9 ;@ L74D38;E: ,)&*" +!&* HA>

BB 01 * #;G CG3@F;F3F;H7 L74D38;E: B:7@AFKB;@9 FAA> 8AD 67H7>AB?7@F3> 4;A>A9K 3@6 6;E73E7 ?A67>;@9 2

" %# )&**"% 0"% HA> BB 01 $ G ,;EG3> B3FF7D@ D75A9@;F;A@ 4K ?A?7@F ;@H3D;3@FE

)%*+"&%*&%%&)$+"&%!&)/HA> BB 01 ( $ 3D3>;5=" ):3@?G93? ;@EF7;@ 1*7JFGD7873FGD7E 8AD ;?397 5>3EE;8;53F;A@ 2

)%*+"&%* &% /*+$* % % /)%+"*HA> BB 01 # D7;?3@! D;76?3@ ! )FA@73@6( &>E:7@#**""+"&% % )**"&% )* 7>?A@F -36EIADF: @F7D@3F;A@3> DAGB 0 1 * 3EF;7( *;4E:;D3@;3@6! D;76?3@! #$%+*&++"*+" #)%"% %7I.AD=)BD;@97D

2007 IEEE/NIH Life Science Sysytems and Applications Workshop (LISSA 2007)