Biochip array technology immunoassay performance and quantitative ...
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Biochip array technology immunoassay performance and quantitative ...
Apr 23, 2015 - Biochip array technology immunoassay performance and quantitative confirmation of designer piperazines for urine workplace drug testing.
Anal Bioanal Chem (2015) 407:4639–4648 DOI 10.1007/s00216-015-8660-z
RESEARCH PAPER
Biochip array technology immunoassay performance and quantitative confirmation of designer piperazines for urine workplace drug testing Marisol S. Castaneto 1,2 & Allan J. Barnes 1 & Marta Concheiro 1,4 & Kevin L. Klette 3 & Thomas A. Martin 3 & Marilyn A. Huestis 1
Received: 17 February 2015 / Revised: 16 March 2015 / Accepted: 24 March 2015 / Published online: 23 April 2015 # Springer-Verlag Berlin Heidelberg (outside the USA) 2015
Abstract Designer piperazines are emerging novel psychoactive substances (NPS) with few high-throughput screening methods for their identification. We evaluated a biochip array technology (BAT) immunoassay for phenylpiperazines (PNP) and benzylpiperazines (BZP) and analyzed 20,017 randomly collected urine workplace specimens. Immunoassay performance at recommended cutoffs was evaluated for PNPI (5 μg/L), PNPII (7.5 μg/L), and BZP (5 μg/L) antibodies. Eight hundred forty positive and 206 randomly selected presumptive negative specimens were confirmed by liquid chromatography high-resolution mass spectrometry (LC-HRMS). Assay limits of detection for PNPI, PNPII, and BZP were 2.9, 6.3, and 2.1 μg/L, respectively. Calibration curves were linear (R2 >0.99) with upper limits of 42 μg/L for PNPI/PNII and 100 μg/L for BZP. Quality control samples demonstrated imprecision
