Virucidal Activity of Some Disinfectants, Chelatinging and Detergent Agents Used In Egyptian Poultry Industry Against Two Locally Isolated HPAI Circulating In Egypt A. Gamal; E. Ismael; S. Moubarak; H. Hussein; M. Hamoud; M. Zaki; M. A. Metwally a and O. Zahran b a
[email protected]
Introduction
; b
[email protected]
Material and Methods
Virucidal Activity of Some Disinfectants, Clutching and Detergent Agents Used In Egyptian Poultry Industry against Two Locally Isolated HPAI Circulating In Egypt
Virucidal Activity of Some Disinfectants, Clutching and Detergent Agents Used In Egyptian Poultry Industry against Two Locally Isolated HPAI Circulating In Egypt
Results First strain (A/Ck/Egypt/13VIR952/ 2011) variant DISNFECTANT
VIRKON S 5 MIN 10 MIN 15 MIN 30 MIN
ALDEKOL 5 MIN 30 MIN
BIOSENTERY 904 5 MIN 30 MIN
BIOSENTERY IODINE 5 MIN 30 MIN
Positive Control (PC)*/ (log 10)
4.5
4.9
4.6
4.5
Average Titer (Ta)**/ (log 10)
1.2
1.2
1.2
1.2
Neutralizing index (NI)***
3.3
3.7
3.4
3.3
Second strain (A/chicken/Egypt/13VIR3729-4/2013) classical
Recoverable viruses
Nil
Nil
Nil
Nil
Positive Average Titer Neutralizing Recoverable (Ta)**/ DISNFECTANT Control (PC)*/ index viruses (log 10) (log 10) (NI)***
VIRKON S 5 MIN 10 MIN 15 MIN 30 MIN
4.6
1.2
3.4
Nil
ALDEKOL 5 MIN 30 MIN
4.5
1.2
3.3
Nil
BIOSENTERY 904 5 MIN 30 MIN
4.6
1.2
3.4
Nil
BIOSENTERY IODINE 5 MIN 30 MIN
4.6
1.2
3.4
Nil
Virucidal Activity of Some Disinfectants Chelating and Detergent Agents Used In Egyptian Poultry Industry against Two Locally Isolated HPAI Circulating In Egypt
Results
3.8
The effect of different disinfectants on both A/Ck/Egypt/13VIR952/2011 (FA-2) and A/chicken/Egypt/13VIR3729-4/2013 (FA-6) viruses on 5 and 30 min. contact times FA-2
Neutralization Index
3.7
FA-6
3.6 3.5 3.4 3.3 3.2 3.1
5
30 Virkon-S
5
30
Biocentery 904
5
30 Aldekol
Contact time (min) / Disinfectants
5
30 min. Iodine
Conclusion
Virucidal Activity of Some Disinfectants, Clutching and Detergent Agents Used In Egyptian Poultry Industry against Two Locally Isolated HPAI Circulating In Egypt
• Most of the tested disinfectants effectively inactivated
HPAI on cement surface (coupons) which was designed specifically to resemble poultry farm floors used in Egypt according to the guidelines of EPA after 5 min contact time. • The exception was (Calcium hypochlorite after 10 min,
sodium hypochlorite, PERAC LEAN 5% ® and Formic acid after 30 min). • When the same disinfectants were tested against the two identified HPAI strains no difference in inactivation ability were found.
