Bph32 , a novel gene encoding an unknown SCR domain-containing protein confers resistance against the brown planthopper in rice
Juansheng Ren1*, Fangyuan Gao1*, Xianting Wu1*, Xianjun Lu1, Lihua Zeng3, Jianqun Lv1, Xiangwen Su1, Hong Luo2, and Guangjun Ren1** 1 Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China 2 Department of Genetics and Biochemistry, Clemson University,110 Biosystems Research Complex, Clemson, SC 29634-0318,USA 3 Sichuan Normal University, Chengdu, 610066, P.R. China *These authors contributed equally to the work. **Corresponding author e-mail:
[email protected] Table S4. Plant material used in this paper. Accession or variety*
origin country or region
121216 China 195B China BC-10-46 Philippines DARMALI Nepal GPNO-25912 El Salvador IR54751-1-2Philippines 44-15-2-3-B IR60 Philippines
taxon
BPH resistance gene**
Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza glaberrima
Bph32 Bph32 Bph32 Bph32 Bph32
3.6 3.6 2.3 4 4
Oryza sativa
Bph32
1
Oryza sativa
Bph32
4.3
Scale
IR64-D-518-6106 IR64-D-518-66IR70 IR78222-20-7148-2-B IR78525-150 IR80314-4-B1-3-B
Philippines
Oryza sativa
Bph32
3
Philippines Philippines
Oryza sativa Oryza sativa
Bph32 Bph32
3 4.3
Philippines
Oryza sativa
Bph32
3
Philippines
Oryza sativa
Bph32
2.3
Philippines
Oryza sativa
Bph32
3
Oryza sativa
Bph32
3
Oryza latifolia
Bph32
IR80340-23-BPhilippines 12-6-B Oryza Bolivia # latifolia Ptb33 India SHZ-2 Tin-Tin95 Myanmar UA-1012 Niger YuanhongChina # Wildrice Zengxiang Myanmar IR65482-4Philippines 136-2-2-B Bombon WC2811 BERLIN Padi Pohon Batu Italica Carolina KRASNODAR SKIJ 3352 Hi Muke Chuanxiang29 B JIN23B WC-3532 LUSITANO
p
Oryza sativa Oryza sativa Oryza sativa
Bph32, Bph3 , BPH26 Bph32 Bph32 Bph32
ryza nivara
Bph32
Oryza sativa
Oryza sativa
0.6 2.3 1.3 3
1
Spain Micronesia Costa Rica
Oryza sativa Oryza sativa Oryza sativa
Bph32 84 Bph10 ， 389B bph32 389B bph32 389B bph32 389B bph32
Malaysia
Oryza sativa
bph32
389B
9
Poland
Oryza sativa
bph32
389B
9
Russian Federation Kazakhstan China China Peru Portugal
Oryza sativa
bph32
389B
9
Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa
bph32 389B bph32 389B bph32 389B bph32 389B bph32
389B
9 9 9 9 9
Oryza sativa
3 9 9 9
389B
389B China SORNAVARI Mali
Oryza sativa Oryza sativa
Rathu Heenati Sri Lankan
Oryza sativa
Kasalath (Ka) Stg 9544-32 BritishHondurasCreole
India United States
Oryza sativa Oryza sativa
bph32
ka
bph32
ka
Belize
Oryza sativa
bph32
ka
9
Bombilla THAVALU Ardito Toga IR80310-12-B1-3-B
Spain Sri Lanka Italy India
Oryza sativa Oryza sativa Oryza sativa Oryza sativa
bph32 ka
9 9 9 9
Philippines
Oryza sativa
Unknown,bph32
Oryza sativa
bph32
SK
9
Oryza sativa Oryza sativa
bph32
SK
bph32
SK
9 9
Oryza sativa
bph32
p
9
Oryza sativa
bph32
TC
9
Oryza sativa Oryza sativa Oryza sativa
bph32
TC
bph32
TC
bph32
TC
9 9 9
Oryza rufipogon
bph32
Or
Red Khosha Afghanistan Cerma Safut-Khosha Afghanistan P-35 India WAB450-24-23-P33-HB Cote D'Ivoire IRRI-IRGCPhilippines 101508 FIROOZ Iran TCHAMPA Iran Nam Dawk Thailand Mai Oryza India rufipogon Oryza glumipatula TOg-7147 Montakcl B805D-MR16-8-3 Gasym-Hany Dara
Liberia Senegal Egypt Indonesia Azerbaijan Indonesia
9 9
bph32 389B bph32 Bph3 ， 389B bph32
1 9 9
bph32 ka bph32 ka bph32 ka ka
3
Oryza Or bph32 glumipatula Oryza glaberrima bph32 Or Or Oryza sativa bph32
9 9
Oryza sativa
bph32
Or
9
Oryza sativa Oryza sativa
bph32
Or
bph32
Or
9 9
IR77186-122Philippines 2-2-3 IR77542-551-Philippines 1-1-1-1-2 Y58S China TOg 6804 Nigeria CG 14 Cote D'Ivoire IR 58614-B-BPhilippines 8-2 E B Gopher United States WarrangalCulture-1252 India Sel. No. 388 Uruguay Chin Chin Panama Eiko France NORIN-8 Japan PI 298967-1 Australia NSGC 5953 Myanmar WAB462-10-31 Cote D'Ivoire ARC-10633 India HG-24 Burkina Faso TOG-7102 Mali TOg-7267 Cameroon Romeno Portugal TOg-7161a Senegal HKG 98 Mali Karabaschak Bulgaria TOg-7257 Chad II-32B China CHENGHUI44 China 8 CHENGHUI32 China 03 ASWINA-330 Bangladesh
Oryza sativa
Unknown,bph32
3
Oryza sativa
Unknown,bph32
3
Oryza sativa bph32 Oryza glaberrima bph32 Oryza glaberrima bph32
9 9 9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa
bph32 bph32 bph32 bph32 bph32 bph32
9 9 9 9 9 9
Oryza sativa
bph32
9
Oryza sativa Oryza sativa Oryza glaberrima Oryza glaberrima Oryza sativa Oryza glaberrima Oryza sativa Oryza sativa Oryza sativa Oryza sativa
bph32 bph32 bph32 bph32 bph32 bph32 bph32 bph32 bph32 bph32
9 9 9 9 9 9 9 9 9 9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Vary-TarvaOsla Somewake BHIM-DHAN MOROBEREK AN Won-Son-ZoNo.-11 IARI-6621 IR75870--8-12-B-6-1-1-B Luxiang90B Lemont TOg 7131 Celiaj CAROLINO 164 163B Niwahutaw Mochi Angkrang CSORNUJ R 67 N-2703 WIR 3039 K8C-263-3 Chun 118-33 Jumli dhan INIAP 7 P 79 ChacareiroUruguay Doble-Carolina Hsin Hsing Pai Ku
Oryza sativa
bph32
9
Oryza sativa Oryza sativa
bph32 bph32
9 9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Philippines
Oryza sativa
Unknown,bph32
3
China United States Senegal Azerbaijan
Oryza sativa Oryza sativa Oryza glaberrima Oryza sativa
bph32 bph32 bph32 bph32
9 9 9 9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa
bph32 bph32 bph32 bph32 bph32 bph32 bph32 bph32 bph32 bph32
9 9 9 9 9 9 9 9 9 9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Portugal Japan Nepal Guinea Korea India
Chad China Japan Cambodia Hungary Senegal Nepal Tajikistan Suriname China Nepal Ecuador India Uruguay Uruguay Taiwan
Sapundali Local India TD 70 Thailand IR 2061-214-23 Philippines DJ-24 Bangladesh TAINUNG 45 Taiwan HB-6-2 Hungary 9311 Nipponbare Japan Taichung Native (TN1) Taiwan
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa
bph32
9
Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa
bph32 bph32 bph32 bph32 bph32
9 9 9 9 9
Oryza sativa
bph32
9
*Rice marterials were from International Rice Research Institute, Dale Bumpers National Rice Research Center, US Department of Agriculture-Agricultural Research Service (USDA-ARS) and Sichuan Academy of Agricultural Sciences.# Leaves were collected from Guangxi Academy of Agricultural Sciences. **Only major BPH resistance genes are listed.
