B.Sc.Biotechnology Semester III & IV Syllabus.pdf

Curriculum, B.Sc. Biotechnology, Semester III & IV

SAURASHTRA UNIVERSITYRAJKOT (Faculty of Science)

COURSE STRUCTURE & SYLLABUS FOR UNDERGRADUATE PROGRAMME IN

BIOTECHNOLOGY (CORE COURSE FOR SEMESTER III & IV) As per Choice Based Credit System (CBCS) Recommended by University Grant Commission (UGC) Government of India, New Delhi)

Effective from June - 2010

Saurashtra University, Rajkot, Gujarat (INDIA) 1 of 16


SKELETO N OF CO MPLETE COURSE CONTE NT O F UNDE R GRADUATE BIOTECHNOL OGY SEME STER I -to VI SEMESTER

I

II

III

IV

V

VI

PAPER NO. & CODE

TITLE OF THE PAPER

CREDIT

BT-101 (Theory)

Introduction to Biotechnology and Cell Biology

4

BT -101 (Practical)

-do-

3

BT -201 (Theory)

Fundamentals of Bioche mistry , Biocomputing and Biostatistics

4

BT -201 (Practical)

-do-

3

BT -301 (Theory)

Basic aspects of cellular metabolism

4

BT -301 (Practical)

-do-

3

BT -401 (Theory)

Environmental Biotechnology

4

BT -401 (Practical)

-do-

3

BT -501 (Theory)

Bioprocess and Biochemical Engineering

4

BT -501 (Practical)

-do-

3

BT -502 (Theory)

Genetics and Molecular Biology

4

BT -502 (Practical)

-do-

3

BT -503 (Theory)

Immunology

4

BT -503 (Practical)

-do-

3

BT -601 (Theory)

Principles of Biotechnology Applied to Plants and Animals

4

BT -601 (Practical)

-do-

3

BT -602 (Theory)

Analytical Techniques in Biotechnology

4

BT -602 (Practical)

-do-

3

BT -603 (Theory)

Advanced Molecular Techniques and Bioinformatics

4

BT -603 (Practical)

-do-

3



COURSE STRUCTURE FOR UG PROGRAMME BIOTECHNOLOGY- 301 SEMESTER- III Hours/week Credit

Exam hours

Internal External marks marks

Total marks

Semester

Course

Title

III

BT-301 (Theory)


6

4

3

25

50

75

III

BT-301 (Practical)


6

3

4

Nil

25

25

Total credits

7

Total marks

100

General instructions 1. 2. 3. 4. 5. 6.

The medium of instruction will be english for theory and practical courses There will be 6 lectures / week / theory paper / semester. Each lecture will be of 55 mins. There will be 2 practical / week / paper / batch. Each practical will be of 3 periods Each semester theory paper will be of “five” units. There will be 60 hrs. of theory teaching / paper / semester. Each theory paper / semester will be of 75 marks. There will be 25 marks for internal evaluation and 50 marks for external evaluation. Each practical paper / semester will be of 25 marks. So, total marks of theory and practical for each paper will be 100 i.e. 75 + 25 = 100)

Saurashtra University, Rajkot, Gujarat (INDIA)

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Skeleton of Theory Examination Paper - External) (SEMESTER – III) SECTION - I

Marks – 1x30 = 30

Ques. 1 Attempt the following Multiple Choice Questions in the given time period (The questions will be of MCQ type each carrying one mark. All the units will be covered in this section.) SECTION - II Ques.1 Answer specifically- (attempt any three out of six)

Ques.2

Marks – 40 Units 1 to 5

3x2= 6 Marks

Short Questions - (attempt any three out of six)

Units 1 to 5

3x3= 9 Marks

Answer in detail – (attempt any one out of three)

Units 1 to 5

5x1= 5 Marks

Answer specifically- (attempt any three out of six)

Units 1 to 5

3x2= 6 Marks


Units 1 to 5

3x3= 9 Marks


Units 1 to 5

5x1= 5 Marks

General Instructions: 1) Time duration of each Theory Paper will be of Three (3) Hrs. 2) Total Marks of each Theory Paper will be 70 Marks. 3) There will be TWO Sections in Question Paper. Section ONE will be of Objective type Questions while Section TWO will be Descriptive. There will be TWO Questions in this section with internal option.

4) Section ONE will be of 30 Marks and Section TWO of 70 marks. 5) Both the Sections are compulsory and all questions in Section TWO are compulsory.

6) Both the sections are to be written in the separate answer sheet.



Skeleton & Question Paper for Practical Examination (SEMESTER – III) SECTION- I: EXAMINER –I (EXTERNAL) Ex. No.

Detail of Exercise

Marks

Day of exercise

1

Perform Quantitative Estimation Of Protein By Biuret method/DNA by DPA method/RNA by Orcinol method/Reducing sugar by Nelson Somogyi method

07

1st Day

2

Certified Journal Submission

03

1st Day

3

Viva-voce

02

1st Day

Total Marks

12

SECTION- II: EXAMINER –II (INTERNAL) Detail of Exercise

Marks

Day to begin the exercise

1

Perform the assay of given enzyme

07

1st Day

2

Spotting

04

1st Day

3

Viva-voce

02

1st Day

Ex. No.

Total Marks

13

Instructions to the candidates 1. All the students have to remain present at the examination centre 15 minutes before the scheduled time for examination. 2. Students have to carry with them certified journal, I-card or examination receipt, and other necessary requirements for examination. 3. Candidate should not leave the laboratory without the permission of examiner. 4. Use of calculator is allowed but the use of mobile phones is strictly prohibited. 5. The candidate has to leave the laboratory only after the submission of all the answer sheets of the exercises performed.



Saurashtra University, Rajkot Sy llabu s for Biotechn ology S emester - III (With effect from June 2011)

B T - 3 0 1 - Basic aspects of Cellular Metabolism ( Th e o ry ) UNIT – 1 Teachi ng H rs. –1 2, Total Credit – (T 1.1

0.8)

Enzymes: - General properties, Nomenclature and Classification. Biocatalyst and chemical catalyst, Coenzymes and Cofactors.

1.2

Catalytic Mechanism (Proximity and Orientation effects, Acid base Catalysis, Covalent Catalysis and Metal ion catalysis and Transition state analog).

1.3

Enzyme Kinetics (derivation of Michaelis–Menten constant, linear transformation of the equation).

1.4

Enzyme Inhibition: Mechanism and Types (Irreversible and Reversible).

1.5

Allosteric Enzymes and Isoenzymes.

UNIT – 2 (T Teachi ng H rs. –1 2, Total Credit –

0.8)

2.1

Mechanism of Enzyme Regulation: Covalent and Allosteric Regulation.

2.2

Protein Structure: The Primary level of protein structure, Secondary structure (α Helix, β sheet and fibrous proteins), Tertiary structure and Quaternary structure.

2.3

Protein Folding (Role of Disulphide & Molecular Chaperons), Example of Ribonucleases A.

2.4

Overview of Protein- Protein Interaction & DNA- Protein Interaction.

2.5

Protein Sequencing.

UNIT – 3 Teachi ng H rs. –1 2, Total Credit – (T 3.1

0.8)

Introduction to Metabolism, Types of Metabolic reactions: Fermentation- Anaerobic Fate of Pyruvate

3.2

Carbohydrate Metabolism: Glycolysis, Acetyl CoA formation (PDH complex),

3.3

Oxidative processes: Citric Acid cycle & Pentose Phosphate pathway

3.4

Electron transport and Oxidative Phosphorylation.

3.5

Gluconeogenesis: Carbohydrate biosynthesis



UNIT – 4 (T Teachi ng H rs. –1 2, Total Credit – 4.1

0.8)

Amino acid Metabolism: Transamination, Decarboxylation, Oxidative deamination &

Urea cycle 4.2

Oxidation of Lipid via β -oxidation.

4.3

Photosynthesis in Plants.

4.4

Photosynthesis in Bacteria.

4.5

Inborn errors in Metabolism.

UNIT – 5 (T Teachi ng H rs. –1 2, Total Credit – 5.1

0.8)

Role of membrane in transportation of molecules and signal Transduction: Membrane structure and Fluid Mosaic Model

5.2

Movement of molecule across membrane: The mechanisms of transportation

5.3

Membrane proteins and their role in transportation and transduction of signals; domains and micro domains of membrane proteins an basic understanding

5.4

Signal transduction, cascades: The G-Proteins – membrane receptors and detail mechanism of message transfer and secondary messenger

5.5

Role of hormones as messenger in regulation of cellular metabolism



B T - 3 0 1 - Basic Aspects of Cellular Metabolism (Practical)

Practical hrs.-3 hrs/ da y for 2 day s/Week. T o t a l credit- 3 Total 6 hrs./week

Exp.1. To demonstrate working operations of spectrophotometer. Exp.2. Estimation of Protein by Biuret method. Exp.3. Estimation of Reducing Sugar by Nelson- Somogyi method Exp.4. Estimation of RNA by Orcinol method. Exp. 5 Estimation of DNA by DPA method. Exp. 6 Estimation of Chlorophyll. Exp. 7, 8, 9

Assaying of various enzymes (any three): a) Amylases by KI-I2 method. b) Phenol oxidase (Potato). c) Phosphatases d) Urease. e) Invertase by GOD/POD and DNSA method. f) Proteolytic enzymes (Trypsin or Pepsin). g) Lipases (Germinating castor seeds).

Exp.10,11,12 Enzyme Kinetics: a) Effect of Substrate concentration (Determination of Km and Vmax). b) Determine temperature optima of the enzyme. c) Effect of pH on enzyme activity. Exp. 13

Effect of Enzyme Inhibitors on Enzyme Activity.



References 1. Lenhinger. Principles of Biochemistry, Nelson & Cox, 4th Edition. 2. Voet & Voet Donald. 3rd Edition. Fundamentals of Biochemistry, J/W. 3. Mathews, Van Holde, Biochemistry, 3rd Edition Pearson Education. 4. Garret and Grisham, Biochemistry, Thomsan Edition, 3rd Edn. 5. U Satyanarayan, Biochemistry, 3rd Edn, Books and Allied Pvt. Ltd. 6. Salisburry and Rose, Plant Physiology, 4th Edn, Wadsworth Pub. 7. Arthur M. Lask, Introduction to Protein Science, Oxford ublication. 8. Stryer – Biochemistry. W.H.Freeman & Co. 9. Price & Steven, Fundamentals of Enzymology,3rd Edition 10. Cohn and Stumph. Outline of Biochemistry. Wiley eastern. 11. Creigntion, proteins: Structure & Molecular Properties, Freeman Pub. 12. Zube’s Biochemistry.4th Edition Macmillan. 13. Switzer and Garrity. Experimental Biochemistry WH Freeman.2nd Edition 14. Hames and Hooper. 2000. Instant notes in Biochemistry. BIOS Sci. Publ. 15. Smith G.Biotechnology. Cambeidge Univ. Press. 16. Geoffrey Cooper. The cell with CD- Rom. Sinauer Asso. Incorp. 17. Elliott & Elliot.3rd Edition Biochemistry and molecular bilogy. 18. Seidman and Moore. 2000. Basic laboratory methods for biotechnology. Longman 19. Boyer, Concepts in biochemistry. Thomson 20. A.V.S.S. Rama Rao, A Text book of Biochemistry, , UBS Publisher 21. S.R. Thimmaiah, Standard methods in Biochemical Analysis, Kalayani Pub. 22. Sawhney and Randhir Singh, Introductory Practical Biochemistry, Narosa Pub. 23. Beedu Sashidar Rao & Vijay Deshpande, Experimental Biochemistry, I K Int. Pvt. Ltd. 24. Plumner. An introduction to practical Biochemistry,3rd Edition 25. J.Jayraman. Lab Manual in Biochemistry.



COURSE STRUCTURE FOR UG PROGRAMME BIOTECHNOLOGY- 401 SEMESTER- IV Hours/week Credit

Exam Internal External Total hours marks Marks Marks

Semester

Course

Title

IV

BT-401 (Theory)


6

4

3

25

50

75

IV

BT-401 (Practical)


6

3

4

Nil

25

25

Total credits

7

Total marks

100

General Instructions 1. 2. 3. 4. 5. 6.

The medium of instruction will be english for theory and practical courses There will be 6 lectures / week / theory paper / semester. Each lecture will be of 55 mins. There will be 2 practical / week / paper / batch. Each practical will be of 3 periods Each semester theory paper will be of “five” units. There will be 60 hrs. of theory teaching / paper / semester. Each theory paper / semester will be of 75 marks. There will be 25 marks for internal evaluation and 50 marks for external evaluation. Each practical paper / semester will be of 25 marks. So, total marks of theovry and practical for each paper will be 100 i.e. 75 + 25 = 100)


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Skeleton of Theory Examination Paper -External (SEMESTER – IV) SECTION - I

Marks – 1x30 = 30

Ques. 1 Attempt the following Multiple Choice Questions in the given time period (The questions will be of MCQ type each carrying one mark. All the units will be covered in this section.) SECTION - II Ques.1

Ques.2

Marks – 40


Units 1 to 5

3x2= 6 Marks


Units 1 to 5

3x3= 9 Marks


Units 1 to 5

5x1= 5 Marks


Units 1 to 5

3x2= 6 Marks


Units 1 to 5

3x3= 9 Marks


Units 1 to 5

5x1= 5 Marks

General Instructions: 1) Time duration of each Theory Paper will be of Three (3) Hrs. 2) Total Marks of each Theory Paper will be 70 Marks. 3) There will be TWO Sections in Question Paper. Section ONE will be of Objective type Questions while Section TWO will be Descriptive. There will be TWO Questions in this section with internal option.

4) Section ONE will be of 30 Marks and Section TWO of 70 marks. 5) Both the Sections are compulsory and all questions in Section TWO are compulsory. 6) Both the sections are to be written in the separate answer sheet.


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Skeleton & Question Paper for Practical Examination (SEMESTER – IV) SECTION- I: EXAMINER –I (EXTERNAL) Ex. No.

Detail of Exercise Estimation of Nitrate/Nitrite/Chloride/Sulphate/PhosphorousPO4 of the given sample

1

Marks

Day of exercise

07

1st Day

2

Certified Journal Submission

03

1st Day

3

Viva-voce

02

1st Day

Total Marks

12

SECTION- II: EXAMINER –II (INTERNAL) Ex. No. 1

Detail of Exercise Check the DO/Ca-Mg of given water sample

Marks Day to begin the exercise 07

1st Day

2

Spotting

04

1st Day

3

Viva-voce

02

1st Day

Total Marks

13

Instructions to the Candidates 1. All the students have to remain present at the examination centre 15 minutes before the scheduled time for examination. 2. Students have to carry with them Certified journal, I-card or examination receipt, and other necessary requirements for examination. 3. Candidate should not leave the laboratory without the permission of examiner. 4. Use of calculator is allowed but the use of Mobile phones is strictly prohibited. 5. The candidate has to leave the laboratory only after the submission of all the answer sheets of the exercises performed.


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Saurashtra University, Rajkot Syllabus for Biotechnology Semester - IV (With effect from June 2011) B T - 4 0 1 - Environmental Biotechnology

(Theory) UNIT: 1 Teaching Hrs. –12, Total Credit – 0.8) (T 1.1 Terrestrial Biomes: - Deserts, Grasslands, Tundra & Forests and Aquatic Biomes: Freshwater & Saline Ecosystem 1.2 Biogeochemical Cycles: Nitrogen, Carbon & Sulfur cycle 1.3 Biodiversity: - Factors affecting biodiversity, Biodiversity conservation 1.4 Interaction within, between & among populations 1.5 Population Ecology, Population characteristics, Models of population growth and Interactions

UNIT: 2 (T Teaching Hrs. –12, Total Credit – 0.8) 2.1

Diversity of metabolic processes among bacteria. Metabolism of organic & Inorganic matter

2.2

Overview of Biodegradation

2.3

Degradation of Basic Structures found in Hydrocarbons & Xenobiotics

2.4

Biodegradation of Xenobiotics, PCBs (Poly Chlorinated Biphenyls), DDT, Nitrobenzene

2.5

Biomagnifiction

UNIT: 3 (T Teaching Hrs. –12, Total Credit – 0.8) 3.1 Physical, chemical & Biological properties of water and Wastewater 3.2 Primary & Secondary treatment, (Biological oxidation processes), E.g. Trickling Filters & Activated sludge 3.3 Tertiary treatment processes 3.4 Conventional Air Pollutants & Acid rain & Acid mine drainage 3.5 An overview of process of Bioremediation Saurashtra University, Rajkot, Gujarat (INDIA)

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UNIT: 4 (T Teaching Hrs. –12, Total Credit–

0.8)

4.1 Treatment of solid wastes (Anaerobic digestion and composting) 4.2 Biofertilizers 4.3 Bioplastics 4.4 Bioleaching 4.5 Biocontrol

UNIT: 5 (T Teaching Hrs. –12, Total Credit–

0.8)

5.1 Early life forms and processes of formation of the present complex life forms 5.2 Theories of origin of organic evolution; Charles Darwin, Lamarck and Wallace 5.3 Understanding a species, morphological and biological species concept, theory natural selection 5.4 Types of reproductive isolation and species formation, allopathic, sympatric and paratactic speciation 5.5 An overview of phylogenetic studies


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B T - 4 0 1 - Environmental Biotechnology (Practical)

Practical hrs.-3hrs/day for 2 days/Week. T o t a l credit- 3 Total 6 hrs./week

Analysis of Waste Water Exp. 1. Color, Turbidity, Odor and pH Exp. 2. TS, TDS and TSS Estimation Exp. 3. NH4-N Estimation Exp. 4. NO2-N Estimation Exp. 5. NO3-N Estimation Exp. 6. Organic nitrogen Estimation Exp. 7. Chloride Estimation Exp. 8. Sulfate Estimation Exp. 9. Ca-Mg Hardness Exp. 10. Phosphorus Phosphate Estimation Exp. 11. Dissolved oxygen (DO) Exp. 12. Biochemical Oxygen Demand (BOD) Exp. 13. Chemical Oxygen Demand (COD) Exp. 14. Bacteriological analysis by MPN technique Exp. 15. Isolation of nitrogen fixers from soil


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LIST OF REFERENCES 1. Manoj Tiwari & Kapil Khulbe, Environmental studies, IK International 2. Bimal Bhattacharya & Rintu Banerjee, Environmental Biotechnology, Oxford Pub. 3. H.R. Singh, Environmental Biology, S. Chand Pub. 4. P.D. Sharma, Environmental Microbiology, Naroosa Pub. 5. Nuzhat Ahmed, Industrial And Environmental Biotechnology, Horizon press 6. S.K.Agrawal, Advanced Environmental Biotechnology, APH pub. 7. Gareth M. Evans & Judith C. Furlong, Environmental Biotechnology, Wiley pub. 8. K. Omasa, Pollution & Plant Biotechnology, Springer Int Edn 9. Indu Shekhar Thakur, Environmental Biotechnology, IK International 10. William P.Cunningham, Environmental Science, McGraw Hill 11. Pradipta Kumar Mohapatra, Textbook of Environmental Biotechnology, IK Int. 12. A. Mackenzie, Instant notes in Ecology, Viva books Pvt Ltd 13. Rajvaidhya, Environmental Biochemistry, APH Pub 14. Ahmed, Industrial & Envi. Biotech, Horizon 15. Bitton, Wastewater Microbiology - 2 ed, Wiley 16. Purohit Shammi, Environmental Sciences, Student Edi 17. Eugene Odum ,Ecology, Oxford 18. Gerba & Pepler, Environment microbiology 19. Hammer. Water and Wastewater technology. Prentice-Hall. 20. APHA. Water and Wastewater analysis. 21. Scragg, A. H. 1999. Environment Biotechnology. Longman. 22. Rittman & Mc Carthy. Environ. Biotechnology. Principles & application. McGraw-Hill. 23. N.P.Cheremisinoff. 1999. Biotechnology for waste and wastewater treatment. Noyes Pub. 24. Michael Heal. (Ed). Environ monitoring & biodiagnostics of hazardous Contaminants. 25. Milton, Wainwright. 1999. An Introdution to Environ. Biotechnology. Kluwer Academy.


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