C1(S6) C2(S15) C3 C4 C5 C6(S5) C7 C8 C9(S4) C10(S7) C11 C12 ...

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AtMYB75. AtMYB114. AtMYB113. AtMYB90. NtAn2. PhAN2. LeAnt1. VvMYBA1. VvMYBA2. ROSEA1. GhMYB10. MdMYB10. PyMYB10. GmMYB096.
A C1(S6)

C2(S15) C3 C4 C5 C6(S5) C7 C8

C9(S4)

C10(S7)

C11

C12 C13 C14(S13)

C15

C16

B AtMYB75 AtMYB75 AtMYB114 AtMYB114 AtMYB113 AtMYB113 AtMYB90 AtMYB90 NtAn2 NtAn2 PhAN2 PhAN2 LeAnt1 LeAnt1 VvMYBA1 VvMYBA1 VvMYBA2 VvMYBA2 ROSEA1 ROSEA1 GhMYB10 GhMYB10 MdMYB10 MdMYB10 PyMYB10 PyMYB10 GmMYB096 GmMYB096 GmMYB080 GmMYB080 GmMYB227 GmMYB227 GmMYB008 GmMYB008 GmMYB241 GmMYB241 IbMYB1 IbMYB1 GmMYB155 GmMYB155 GmMYB192 GmMYB192 AtMYB82 AtMYB82 AtMYB66 AtMYB66 AtMYB0 AtMYB0 AtMYB23 AtMYB23 VvMYB5a VvMYB5a VvMTB5b VvMTB5b AtMYB5 AtMYB5 OsMYB4 OsMYB4 ZmC1 ZmC1 ZmPL ZmPL OsC1 OsC1 GmMYB047 GmMYB047 GmMYB115 GmMYB115 GmMYB058 GmMYB058 GmMYB127 GmMYB127 GmMYB045 GmMYB045 GmMYB024 GmMYB024 GmMYB103 GmMYB103 GmMYB191 GmMYB191 LjTT2b LjTT2b LjTT2c LjTT2c LjTT2a LjTT2a GmMYB193 GmMYB193 GmMYB205 GmMYB205 AtMYB123 AtMYB123 GmMYB033 GmMYB033 GmMYB195 GmMYB195 GmMYB136 GmMYB136 GmMYB175 GmMYB175 GmMYB178 GmMYB178 GmMYB194 GmMYB194 FaMYB1 FaMYB1 GmMYB170 GmMYB170 GmMYB203 GmMYB203 GmMYB177 GmMYB177 GmMYB211 GmMYB211 GmMYB106 GmMYB106 GmMYB006 GmMYB006 GmMYB141 GmMYB141 HvMYB5 HvMYB5 AtMYB3 AtMYB3 AtMYB6 AtMYB6 AtMYB8 AtMYB8 Zm38 Zm38 Zm42 Zm42 AtMYB7 AtMYB7 AtMYB32 AtMYB32 GmMYB002 GmMYB002 PhMYB4 PhMYB4 AtMYB4 AtMYB4 GmMYB005 GmMYB005 GmMYB042 GmMYB042 SbY1 SbY1 ZmP1 ZmP1 ZmP2 ZmP2 GmMYB176 GmMYB176 GmMYB190 GmMYB190 GmMYB200 GmMYB200 VvMYBF1 VvMYBF1 SlMYB12 SlMYB12 AtMYB111 AtMYB111 AtMYB11 AtMYB11 AtMYB12 AtMYB12 GmMYB174 GmMYB174 GmMYB204 GmMYB204 GmMYB197 GmMYB197 GmMYB198 GmMYB198 GmMYB098 GmMYB098 GmMYB122 GmMYB122 GmMYB116 GmMYB116 GmMYB129 GmMYB129 AtMYB20 AtMYB20 AtMYB43 AtMYB43 AtMYB85 AtMYB85 AtMYB42 AtMYB42 GmMYB097 GmMYB097 GmMYB114 GmMYB114 GmMYB101 GmMYB101 GmMYB126 GmMYB126 AtMYB99 AtMYB99 Pp2Pp2 PtMYB1 PtMYB1 AtMYB40 AtMYB40 GmMYB124 GmMYB124 GmMYB238 GmMYB238 GmMYB239 GmMYB239 GmMYB163 GmMYB163 GmMYB232 GmMYB232 GmMYB165 GmMYB165 GmMYB209 GmMYB209 AtMYB103 AtMYB103 GmMYB185 GmMYB185 GmMYB213 GmMYB213 GmMYB184 GmMYB184 GmMYB009 GmMYB009 GmMYB187 GmMYB187 AtMYB55 AtMYB55 GmMYB094 GmMYB094 GmMYB039 GmMYB039 GmMYB023 GmMYB023 GmMYB214 GmMYB214 PtMYB8 PtMYB8 AtMYB50 AtMYB50 AtMYB61 AtMYB61 HvMYB33 HvMYB33 AtMYB86 AtMYB86 GmMYB189 GmMYB189 GmMYB186 GmMYB186 GmMYB057 GmMYB057 GmMYB162 GmMYB162 GmMYB107 GmMYB107 GmMYB121 GmMYB121 GmMYB102 GmMYB102 AtMYB26 AtMYB26 AtMYB67 AtMYB67 GmMYB065 GmMYB065 GmMYB085 GmMYB085 PtMYB4 PtMYB4 GmMYB020 GmMYB020 GmMYB188 GmMYB188 GmMYB179 GmMYB179 GmMYB202 GmMYB202 AtMYB46 AtMYB46 GmMYB015 GmMYB015 GmMYB182 GmMYB182 AtMYB83 AtMYB83 EgMYB2 EgMYB2 GmMYB118 GmMYB118 GmMYB180 GmMYB180 AtMYB13 AtMYB13 AtMYB14 AtMYB14 GmMYB132 GmMYB132 GmMYB201 GmMYB201 NtMYB1 NtMYB1 AtMYB15 AtMYB15 GmMYB158 GmMYB158 GmMYB159 GmMYB159 GmMYB215 GmMYB215 GmMYB223 GmMYB223 GmMYB199 GmMYB199 GmMYB022 GmMYB022 GmMYB134 GmMYB134 GmMYB066 GmMYB066 GmMYB123 GmMYB123 AtMYB72 AtMYB72 AtMYB10 AtMYB10 AtMYB58 AtMYB58 AtMYB63

5’

Length of Exon/Intron of GmMYB Domain

C

Architecture of MYB protein conserved motifs

R2 R3

R2 R3

3

R2 R3

4

5

R2 R3

6

R2 R3

R2 R3

R2 R3

1

1 7 8

R2 R3

R2 R3

R2 R3

R2 R3

1

9

1

10

12 141113

R2 R3

R2 R3

R2 R3

1k

2k

3k

4k

5k 6k

7k

8k

15

D

Expreesion pattern R L F S N

AtMYB75 AtMYB114 AtMYB113 AtMYB90 NtAn2 PhAN2 LeAnt1 VvMYBA1 VvMYBA2 ROSEA1 GhMYB10 MdMYB10 PyMYB10 GmMYB096 GmMYB080 GmMYB227 GmMYB008 GmMYB241 IbMYB1 GmMYB155 GmMYB192 AtMYB82 AtMYB66 AtMYB0 AtMYB23 VvMYB5a VvMTB5b AtMYB5 OsMYB4 ZmC1 ZmPL OsC1 GmMYB047 GmMYB115 GmMYB058 GmMYB127 GmMYB045 GmMYB024 GmMYB103 GmMYB191 LjTT2b LjTT2c LjTT2a GmMYB193 GmMYB205 AtMYB123 GmMYB033 GmMYB195 GmMYB136 GmMYB175 GmMYB178 GmMYB194 FaMYB1 GmMYB170 GmMYB203 GmMYB177 GmMYB211 GmMYB106 GmMYB006 GmMYB141 HvMYB5 AtMYB3 AtMYB6 AtMYB8 Zm38 Zm42 AtMYB7 AtMYB32 GmMYB002 PhMYB4 AtMYB4 GmMYB005 GmMYB042 SbY1 ZmP1 ZmP2 GmMYB176 GmMYB190 GmMYB200 VvMYBF1 SlMYB12 AtMYB111 AtMYB11 AtMYB12 GmMYB174 GmMYB204 GmMYB197 GmMYB198 GmMYB098 GmMYB122 GmMYB116 GmMYB129 AtMYB20 AtMYB43 AtMYB85 AtMYB42 GmMYB097 GmMYB114 GmMYB101 GmMYB126 AtMYB99 Pp2 PtMYB1 AtMYB40 GmMYB124 GmMYB238 GmMYB239 GmMYB163 GmMYB232 GmMYB165 GmMYB209 AtMYB103 GmMYB185 GmMYB213 GmMYB184 GmMYB009 GmMYB187 AtMYB55 GmMYB094 GmMYB039 GmMYB023 GmMYB214 PtMYB8 AtMYB50 AtMYB61 HvMYB33 AtMYB86 GmMYB189 GmMYB186 GmMYB057 GmMYB162 GmMYB107 GmMYB121 GmMYB102 AtMYB26 AtMYB67 GmMYB065 GmMYB085 PtMYB4 GmMYB020 GmMYB188 GmMYB179 GmMYB202 AtMYB46 GmMYB015 GmMYB182 AtMYB83 EgMYB2 GmMYB118 GmMYB180 AtMYB13 AtMYB14 GmMYB132 GmMYB201 NtMYB1 AtMYB15 GmMYB158 GmMYB159 GmMYB215 GmMYB223 GmMYB199 GmMYB022 GmMYB134 GmMYB066 GmMYB123 AtMYB72 AtMYB10 AtMYB58

A C17(S2)

C18 C19 C20(S16)

C21

C22

C23(S9)

C24 C25 C26(S12)

C27(S24) C28 C29(S10) C30

C31(S1)

C32

C33(S14)

GmMYB186 GmMYB186 GmMYB057 GmMYB057 GmMYB162 GmMYB162 GmMYB107 GmMYB107 GmMYB121 GmMYB121 GmMYB102 GmMYB102 AtMYB26 AtMYB26 AtMYB67 AtMYB67 GmMYB065 GmMYB065 GmMYB085 GmMYB085 PtMYB4 PtMYB4 GmMYB020 GmMYB020 GmMYB188 GmMYB188 GmMYB179 GmMYB179 GmMYB202 GmMYB202 AtMYB46 AtMYB46 GmMYB015 GmMYB015 GmMYB182 GmMYB182 AtMYB83 AtMYB83 EgMYB2 EgMYB2 GmMYB118 GmMYB118 GmMYB180 GmMYB180 AtMYB13 AtMYB13 AtMYB14 AtMYB14 GmMYB132 GmMYB132 GmMYB201 GmMYB201 NtMYB1 NtMYB1 AtMYB15 AtMYB15 GmMYB158 GmMYB158 GmMYB159 GmMYB159 GmMYB215 GmMYB215 GmMYB223 GmMYB223 GmMYB199 GmMYB199 GmMYB022 GmMYB022 GmMYB134 GmMYB134 GmMYB066 GmMYB066 GmMYB123 GmMYB123 AtMYB72 AtMYB72 AtMYB10 AtMYB10 AtMYB58 AtMYB58 AtMYB63 AtMYB63 GmMYB025 GmMYB025 GmMYB148 GmMYB148 GmMYB145 GmMYB145 GmMYB152 GmMYB152 AtMYB45 AtMYB45 AtMYB18 AtMYB18 AtMYB19 AtMYB19 GmMYB233 GmMYB233 GmMYB142 GmMYB142 GmMYB149 GmMYB149 GmMYB150 GmMYB150 GmMYB062 GmMYB062 GmMYB034 GmMYB034 GmMYB035 GmMYB035 GmMYB071 GmMYB071 GmMYB095 GmMYB095 GmMYB196 GmMYB196 GmMYB206 GmMYB206 AtMYB17 AtMYB17 GmMYB169 GmMYB169 GmMYB212 GmMYB212 AmMYBML2 AmMYBML2 GmMYB077 GmMYB077 AtMYB16 AtMYB16 GmMYB093 GmMYB093 GmMYB070 GmMYB070 GmMYB092 GmMYB092 AtMYB106 AtMYB106 GmMYB063 GmMYB063 GmMYB052 GmMYB052 AmMYBML3 AmMYBML3 AmMYBML1 AmMYBML1 AtMYB49 AtMYB49 AtMYB39 AtMYB39 GmMYB078 GmMYB078 AtMYB28 AtMYB28 AtMYB76 AtMYB76 AtMYB29 AtMYB29 AtMYB34 AtMYB34 AtMYB51 AtMYB51 AtMYB122 AtMYB122 GmMYB157 GmMYB157 GmMYB171 GmMYB171 GmMYB017 GmMYB017 GmMYB083 GmMYB083 GmMYB210 GmMYB210 GmMYB064 GmMYB064 DcMYB1 DcMYB1 AtMYB102 AtMYB102 AtMYB41 AtMYB41 AtMYB74 AtMYB74 GmMYB061 GmMYB061 GmMYB076 GmMYB076 GmMYB242 GmMYB242 GmMYB079 GmMYB079 GmMYB117 GmMYB117 GmMYB054 GmMYB054 GmMYB059 GmMYB059 AtMYB93 AtMYB93 GmMYB048 GmMYB048 GmMYB053 GmMYB053 AtMYB53 AtMYB53 AtMYB92 AtMYB92 AtMYB9 AtMYB9 AtMYB107 AtMYB107 GmMYB010 GmMYB010 GmMYB069 GmMYB069 GmMYB086 GmMYB086 GmMYB029 GmMYB029 GmMYB073 GmMYB073 GmMYB050 GmMYB050 GmMYB072 GmMYB072 GmMYB153 GmMYB153 GmMYB207 GmMYB207 GmMYB172 GmMYB172 GmMYB173 GmMYB173 AtMYB96 AtMYB96 AtMyb30 AtMyb30 AtMYB94 AtMYB94 GmMYB222 GmMYB222 AtMYB60 AtMYB60 GmMYB160 GmMYB160 GmMYB007 GmMYB007 AtMYB31 AtMYB31 GmMYB004 GmMYB004 GmMYB046 GmMYB046 AtMYB47 AtMYB47 AtMYB95 AtMYB95 GmMYB119 GmMYB119 GmMYB131 GmMYB131 AtMYB35 AtMYB35 AtMYB80 AtMYB80 GmMYB089 GmMYB089 GmMYB144 GmMYB144 GmMYB224 GmMYB224 GmMYB225 GmMYB225 GmMYB221 GmMYB221 GmMYB166 GmMYB166 GmMYB216 GmMYB216 GmMYB167 GmMYB167 GmMYB217 GmMYB217 SlBlind SlBlind AtMYB36 AtMYB36 AtMYB37 AtMYB37 AtMYB38 AtMYB38 GmMYB018 GmMYB018 GmMYB038 GmMYB038 GmMYB164 GmMYB164 GmMYB218 GmMYB218 AtMYB87 AtMYB87 GmMYB013 GmMYB013 GmMYB220 GmMYB220 GmMYB137 GmMYB137 GmMYB219 GmMYB219 AtMYB68 AtMYB68 AtMYB84 AtMYB84 GmMYB168 GmMYB168 GmMYB208 GmMYB208 GmMYB161 GmMYB161 GmMYB040 GmMYB040 GmMYB139 GmMYB139 GmMYB243 GmMYB243 GmMYB244 GmMYB244 GmMYB100 GmMYB100 GmMYB183 GmMYB183 GmMYB113 GmMYB113 AtMYB79 AtMYB79 GmMYB128 GmMYB128 GmMYB135 GmMYB135 AtMYB71 AtMYB71 AtMYB121 AtMYB121 GmMYB108 GmMYB108 GmMYB074 GmMYB074 AtMYB48 AtMYB48 AtMYB59 AtMYB59 GmMYB037 GmMYB037 GmMYB082 GmMYB082 AtMYB27 AtMYB27 GmMYB120 GmMYB120 GmMYB146 GmMYB146 GmMYB027 GmMYB027 GmMYB130 GmMYB130 PsMYB26 PsMYB26 AtMYB21 AtMYB21 AtMYB24 AtMYB24 GmMYB043 GmMYB043 GmMYB026 GmMYB026 GmMYB125 GmMYB125 AtMYB57 AtMYB57

B

5’

Length of Exon/Intron of GmMYB Domain

C

Architecture of MYB protein conserved motifs 17 16

R2 R3

R2 R3

R2 R3

R2 R3

19 20

18

R2 R3

1 21 R2 R3

23

22 \

5

24

1 21 R2 R3 5

R2 R3

R2 R3

R2 R3

R2 R3 R2 R3

26

1 28

1 30

R2 R3

1k

2k

3k

4k

5k 6k

7k

8k

27

31

29

1 32 1

R2 R3 34

R2 R3

25

33

35

3637

38

GmMYB057 GmMYB162 GmMYB107 GmMYB121 GmMYB102 AtMYB26 AtMYB67 GmMYB065 GmMYB085 PtMYB4 GmMYB020 GmMYB188 GmMYB179 GmMYB202 AtMYB46 GmMYB015 GmMYB182 AtMYB83 EgMYB2 GmMYB118 GmMYB180 AtMYB13 AtMYB14 GmMYB132 GmMYB201 NtMYB1 AtMYB15 GmMYB158 GmMYB159 GmMYB215 GmMYB223 GmMYB199 GmMYB022 GmMYB134 GmMYB066 GmMYB123 AtMYB72 AtMYB10 AtMYB58 AtMYB63 GmMYB025 GmMYB148 GmMYB145 GmMYB152 AtMYB45 AtMYB18 AtMYB19 GmMYB233 GmMYB142 GmMYB149 GmMYB150 GmMYB062 GmMYB034 GmMYB035 GmMYB071 GmMYB095 GmMYB196 GmMYB206 AtMYB17 GmMYB169 GmMYB212 AmMYBML2 GmMYB077 AtMYB16 GmMYB093 GmMYB070 GmMYB092 AtMYB106 GmMYB063 GmMYB052 AmMYBML3 AmMYBML1 AtMYB49 AtMYB39 GmMYB078 AtMYB28 AtMYB76 AtMYB29 AtMYB34 AtMYB51 AtMYB122 GmMYB157 GmMYB171 GmMYB017 GmMYB083 GmMYB210 GmMYB064 DcMYB1 AtMYB102 AtMYB41 AtMYB74 GmMYB061 GmMYB076 GmMYB242 GmMYB079 GmMYB117 GmMYB054 GmMYB059 AtMYB93 GmMYB048 GmMYB053 AtMYB53 AtMYB92 AtMYB9 AtMYB107 GmMYB010 GmMYB069 GmMYB086 GmMYB029 GmMYB073 GmMYB050 GmMYB072 GmMYB153 GmMYB207 GmMYB172 GmMYB173 AtMYB96 AtMyb30 AtMYB94 GmMYB222 AtMYB60 GmMYB160 GmMYB007 AtMYB31 GmMYB004 GmMYB046 AtMYB47 AtMYB95 GmMYB119 GmMYB131 AtMYB35 AtMYB80 GmMYB089 GmMYB144 GmMYB224 GmMYB225 GmMYB221 GmMYB166 GmMYB216 GmMYB167 GmMYB217 SlBlind AtMYB36 AtMYB37 AtMYB38 GmMYB018 GmMYB038 GmMYB164 GmMYB218 AtMYB87 GmMYB013 GmMYB220 GmMYB137 GmMYB219 AtMYB68 AtMYB84 GmMYB168 GmMYB208 GmMYB161 GmMYB040 GmMYB139 GmMYB243 GmMYB244 GmMYB100 GmMYB183 GmMYB113 AtMYB79 GmMYB128 GmMYB135 AtMYB71 AtMYB121 GmMYB108 GmMYB074 AtMYB48 AtMYB59 GmMYB037 GmMYB082 AtMYB27 GmMYB120 GmMYB146 GmMYB027 GmMYB130 PsMYB26 AtMYB21 AtMYB24 GmMYB043 GmMYB026 GmMYB125 AtMYB57

D

Expreesion pattern R L F S N

A C34 C34

C35 C35 C36 C36 C37(19) C37(19)

C38(S20) C38(S20)

C39(S18) C39(S18)

C40 C40

C41(S23) C41(S23)

C42(S22) C42(S22)

C43(S21) C43(S21)

C45 C45

C46 C46 C47 C47

GmMYB167 GmMYB167 GmMYB217 GmMYB217 GmMYB217 GmMYB217 SlBlind SlBlind SlBlind SlBlind AtMYB36 AtMYB36 AtMYB36 AtMYB36 AtMYB37 AtMYB37 AtMYB37 AtMYB37 AtMYB38 AtMYB38 AtMYB38 AtMYB38 GmMYB018 GmMYB018 GmMYB018 GmMYB018 GmMYB038 GmMYB038 GmMYB038 GmMYB038 GmMYB164 GmMYB164 GmMYB164 GmMYB164 GmMYB218 GmMYB218 GmMYB218 GmMYB218 AtMYB87 AtMYB87 AtMYB87 AtMYB87 GmMYB013 GmMYB013 GmMYB013 GmMYB013 GmMYB220 GmMYB220 GmMYB220 GmMYB220 GmMYB137 GmMYB137 GmMYB137 GmMYB137 GmMYB219 GmMYB219 GmMYB219 GmMYB219 AtMYB68 AtMYB68 AtMYB68 AtMYB68 AtMYB84 AtMYB84 AtMYB84 AtMYB84 GmMYB168 GmMYB168 GmMYB168 GmMYB168 GmMYB208 GmMYB208 GmMYB208 GmMYB208 GmMYB161 GmMYB161 GmMYB161 GmMYB161 GmMYB040 GmMYB040 GmMYB040 GmMYB040 GmMYB139 GmMYB139 GmMYB139 GmMYB139 GmMYB243 GmMYB243 GmMYB243 GmMYB243 GmMYB244 GmMYB244 GmMYB244 GmMYB244 GmMYB100 GmMYB100 GmMYB100 GmMYB100 GmMYB183 GmMYB183 GmMYB183 GmMYB183 GmMYB113 GmMYB113 GmMYB113 GmMYB113 AtMYB79 AtMYB79 AtMYB79 AtMYB79 GmMYB128 GmMYB128 GmMYB128 GmMYB128 GmMYB135 GmMYB135 GmMYB135 GmMYB135 AtMYB71 AtMYB71 AtMYB71 AtMYB71 AtMYB121 AtMYB121 AtMYB121 AtMYB121 GmMYB108 GmMYB108 GmMYB108 GmMYB108 GmMYB074 GmMYB074 GmMYB074 GmMYB074 AtMYB48 AtMYB48 AtMYB48 AtMYB48 AtMYB59 AtMYB59 AtMYB59 AtMYB59 GmMYB037 GmMYB037 GmMYB037 GmMYB037 GmMYB082 GmMYB082 GmMYB082 GmMYB082 AtMYB27 AtMYB27 AtMYB27 AtMYB27 GmMYB120 GmMYB120 GmMYB120 GmMYB120 GmMYB146 GmMYB146 GmMYB146 GmMYB146 GmMYB027 GmMYB027 GmMYB027 GmMYB027 GmMYB130 GmMYB130 GmMYB130 GmMYB130 PsMYB26 PsMYB26 PsMYB26 PsMYB26 AtMYB21 AtMYB21 AtMYB21 AtMYB21 AtMYB24 AtMYB24 AtMYB24 AtMYB24 GmMYB043 GmMYB043 GmMYB043 GmMYB043 GmMYB026 GmMYB026 GmMYB026 GmMYB026 GmMYB125 GmMYB125 GmMYB125 GmMYB125 AtMYB57 AtMYB57 AtMYB57 AtMYB57 AtMYB62 AtMYB62 AtMYB62 AtMYB62 AtMYB116 AtMYB116 AtMYB116 AtMYB116 GmMYB021 GmMYB021 GmMYB021 GmMYB021 GmMYB044 GmMYB044 GmMYB044 GmMYB044 GmMYB104 GmMYB104 GmMYB104 GmMYB104 GmMYB111 GmMYB111 GmMYB111 GmMYB111 AtMYB112 AtMYB112 AtMYB112 AtMYB112 GmMYB001 GmMYB001 GmMYB001 GmMYB001 GmMYB099 GmMYB099 GmMYB099 GmMYB099 GmMYB016 GmMYB016 GmMYB016 GmMYB016 GmMYB112 GmMYB112 GmMYB112 GmMYB112 GmMYB031 GmMYB031 GmMYB031 GmMYB031 GmMYB133 GmMYB133 GmMYB133 GmMYB133 GmMYB226 GmMYB226 GmMYB226 GmMYB226 AtMYB2 AtMYB2 AtMYB2 AtMYB2 TaPIMP1 TaPIMP1 TaPIMP1 TaPIMP1 GmMYB028 GmMYB028 GmMYB028 GmMYB028 GmMYB110 GmMYB110 GmMYB110 GmMYB110 AtMYB78 AtMYB78 AtMYB78 AtMYB78 AtMYB108 AtMYB108 AtMYB108 AtMYB108 GmMYB014 GmMYB014 GmMYB014 GmMYB014 GmMYB105 GmMYB105 GmMYB105 GmMYB105 AtMYB81 AtMYB81 AtMYB81 AtMYB81 AtMYB104 AtMYB104 AtMYB104 AtMYB104 AtMYB125 AtMYB125 AtMYB125 AtMYB125 GmMYB143 GmMYB143 GmMYB143 GmMYB143 AtMYB97 AtMYB97 AtMYB97 AtMYB97 AtMYB120 AtMYB120 AtMYB120 AtMYB120 AtMYB101 AtMYB101 AtMYB101 AtMYB101 GmMYB075 GmMYB075 GmMYB075 GmMYB075 GmMYB181 GmMYB181 GmMYB181 GmMYB181 GmMYB090 GmMYB090 GmMYB090 GmMYB090 GmMYB109 GmMYB109 GmMYB109 GmMYB109 AtMYB33 AtMYB33 AtMYB33 AtMYB33 AtMYB65 AtMYB65 AtMYB65 AtMYB65 GmMYB055 GmMYB055 GmMYB055 GmMYB055 GmMYB084 GmMYB084 GmMYB084 GmMYB084 OsGAMYB OsGAMYB OsGAMYB OsGAMYB HvGAMYB HvGAMYB HvGAMYB HvGAMYB LtGAMYB LtGAMYB LtGAMYB LtGAMYB GmMYB140 GmMYB140 GmMYB140 GmMYB140 GmMYB151 GmMYB151 GmMYB151 GmMYB151 GmMYB240 GmMYB240 GmMYB240 GmMYB240 AmPHAN AmPHAN AmPHAN AmPHAN GmMYB030 GmMYB030 GmMYB030 GmMYB030 GmMYB154 GmMYB154 GmMYB154 GmMYB154 TaWRS2 TaWRS2 TaWRS2 TaWRS2 ZmPHAN ZmPHAN ZmPHAN ZmPHAN LePHAN LePHAN LePHAN LePHAN AtMYB91 AtMYB91 AtMYB91 AtMYB91 GmMYB049 GmMYB049 GmMYB049 GmMYB049 GmMYB081 GmMYB081 GmMYB081 GmMYB081 AtMYB1 AtMYB1 AtMYB1 AtMYB1 AtMYB25 AtMYB25 AtMYB25 AtMYB25 AtMYB109 AtMYB109 AtMYB109 AtMYB109 AtMYB44 AtMYB44 AtMYB44 AtMYB44 AtMYB77 AtMYB77 AtMYB77 AtMYB77 GmMYB012 GmMYB012 GmMYB012 GmMYB012 GmMYB019 GmMYB019 GmMYB019 GmMYB019 GmMYB011 GmMYB011 GmMYB011 GmMYB011 AtMYB70 AtMYB70 AtMYB70 AtMYB70 AtMYB73 AtMYB73 AtMYB73 AtMYB73 GmMYB041 GmMYB041 GmMYB041 GmMYB041 GmMYB003 GmMYB003 GmMYB003 GmMYB003 GmMYB032 GmMYB032 GmMYB032 GmMYB032 GmMYB036 GmMYB036 GmMYB036 GmMYB036 GmMYB231 GmMYB231 GmMYB231 GmMYB231 GmMYB068 GmMYB068 GmMYB068 GmMYB068 GmMYB087 GmMYB087 GmMYB087 GmMYB087 AtMYB52 AtMYB52 AtMYB52 AtMYB52 PttMYB21a PttMYB21a PttMYB21a PttMYB21a AtMYB54 AtMYB54 AtMYB54 AtMYB54 GmMYB056 GmMYB056 GmMYB056 GmMYB056 GmMYB060 GmMYB060 GmMYB060 GmMYB060 AtMYB69 AtMYB69 AtMYB69 AtMYB69 AtMYB89 AtMYB89 AtMYB89 AtMYB89 AtMYB110 AtMYB110 AtMYB110 AtMYB110 GmMYB230 GmMYB230 GmMYB230 GmMYB230 GmMYB236 GmMYB236 GmMYB236 GmMYB236 AtMYB56 AtMYB56 AtMYB56 AtMYB56 GmMYB228 GmMYB228 GmMYB228 GmMYB228 AtMYB105 AtMYB105 AtMYB105 AtMYB105 AtMYB117 AtMYB117 AtMYB117 AtMYB117 GmMYB229 GmMYB229 GmMYB229 GmMYB229 GmMYB235 GmMYB235 GmMYB235 GmMYB235 GmMYB088 GmMYB088 GmMYB088 GmMYB088 AtMYB64 AtMYB64 AtMYB64 AtMYB64 AtMYB119 AtMYB119 AtMYB119 AtMYB119 AtMYB118 AtMYB118 AtMYB118 AtMYB118 AtMYB98 AtMYB98 AtMYB98 AtMYB98 GmMYB234 GmMYB234 GmMYB234 GmMYB234 AtMYB115 AtMYB115 AtMYB115 AtMYB115 AtMYB22 AtMYB22 AtMYB22 AtMYB22 AtMYB100 AtMYB100 AtMYB100 AtMYB100 GmMYB237 GmMYB237 GmMYB237 GmMYB237 GmMYB3R-1 GmMYB3R-1 GmMYB3R-1 GmMYB3R-1 AtMYB3R3 AtMYB3R3 AtMYB3R3 AtMYB3R3 AtMYB3R5 AtMYB3R5 AtMYB3R5 AtMYB3R5 GmMYB3R-3 GmMYB3R-3 GmMYB3R-3 GmMYB3R-3 GmMYB3R-2 GmMYB3R-2 GmMYB3R-2 GmMYB3R-2 ATMYB3R2 ATMYB3R2 ATMYB3R2 ATMYB3R2 GmMYB3R-4 GmMYB3R-4 GmMYB3R-4 GmMYB3R-4 AtMYB3R1 AtMYB3R1 AtMYB3R1 AtMYB3R1 AtMYB3R4 AtMYB3R4 AtMYB3R4 AtMYB3R4 GmMYB3R-5 GmMYB3R-5 GmMYB3R-5 GmMYB3R-5 GmMYB3R-6 GmMYB3R-6 GmMYB3R-6 GmMYB3R-6 AtMYB88 AtMYB88 AtMYB88 AtMYB88 AtMYB124 AtMYB124 AtMYB124 AtMYB124 GmMYB051 GmMYB051 GmMYB051 GmMYB051 GmMYB138 GmMYB138 GmMYB138 GmMYB138 GmMYB067 GmMYB067 GmMYB067 GmMYB067 GmMYB091 GmMYB091 GmMYB091 GmMYB091 AtMYB3R-like AtMYB3R-like AtMYB3R-like AtMYB3R-like GmMYB147 GmMYB147 GmMYB147 GmMYB147 GmMYB156 GmMYB156 GmMYB156 GmMYB156 AtCDC5 AtCDC5 AtCDC5 AtCDC5 AtMYB4R1 AtMYB4R1 AtMYB4R1 AtMYB4R1 GmMYB4R-1 GmMYB4R-1 GmMYB4R-1 GmMYB4R-1 GmMYB4R-2 GmMYB4R-2 GmMYB4R-2 GmMYB4R-2

B

5’5’

Length of Exon/Intron of GmMYB Domain

C

Architecture of MYB protein conserved motifs 30 30 31 31

11 R2 R3 R2 R3

R2 R3 R2 R3 R2 R3 R2 R3

32 32 33 33

R2 R3 R3 R2

34 34

R2 R3 R3 R2

35 35

R2 R3 R3 R2

R2 R3 R3 R2

R2 R3 R3 R2

36 36

37 39 37 38 38 39

40 40 41 41

R2 R3 R3 R2

11 R2 R3 R2 R3

R2 R3 R3 R2

42 42

R3 R1 R2 R3 R1 R2

43 43

R3 R2 R2 R3

R3 R2 R2 R3

48 48

R1 R2 R2 R3 R1 R2 R2 R3

1k1k 2k2k 3k3k 4k4k 5k5k 6k6k 7k7k 8k8k

45 45 49 49

44 44

46 46 47 47 50 50

GmMYB217 GmMYB217 SlBlind SlBlind AtMYB36 AtMYB36 AtMYB37 AtMYB37 AtMYB38 AtMYB38 GmMYB018 GmMYB018 GmMYB038 GmMYB038 GmMYB164 GmMYB164 GmMYB218 GmMYB218 AtMYB87 AtMYB87 GmMYB013 GmMYB013 GmMYB220 GmMYB220 GmMYB137 GmMYB137 GmMYB219 GmMYB219 AtMYB68 AtMYB68 AtMYB84 AtMYB84 GmMYB168 GmMYB168 GmMYB208 GmMYB208 GmMYB161 GmMYB161 GmMYB040 GmMYB040 GmMYB139 GmMYB139 GmMYB243 GmMYB243 GmMYB244 GmMYB244 GmMYB100 GmMYB100 GmMYB183 GmMYB183 GmMYB113 GmMYB113 AtMYB79 AtMYB79 GmMYB128 GmMYB128 GmMYB135 GmMYB135 AtMYB71 AtMYB71 AtMYB121 AtMYB121 GmMYB108 GmMYB108 GmMYB074 GmMYB074 AtMYB48 AtMYB48 AtMYB59 AtMYB59 GmMYB037 GmMYB037 GmMYB082 GmMYB082 AtMYB27 AtMYB27 GmMYB120 GmMYB120 GmMYB146 GmMYB146 GmMYB027 GmMYB027 GmMYB130 GmMYB130 PsMYB26 PsMYB26 AtMYB21 AtMYB21 AtMYB24 AtMYB24 GmMYB043 GmMYB043 GmMYB026 GmMYB026 GmMYB125 GmMYB125 AtMYB57 AtMYB57 AtMYB62 AtMYB62 AtMYB116 AtMYB116 GmMYB021 GmMYB021 GmMYB044 GmMYB044 GmMYB104 GmMYB104 GmMYB111 GmMYB111 AtMYB112 AtMYB112 GmMYB001 GmMYB001 GmMYB099 GmMYB099 GmMYB016 GmMYB016 GmMYB112 GmMYB112 GmMYB031 GmMYB031 GmMYB133 GmMYB133 GmMYB226 GmMYB226 AtMYB2 AtMYB2 TaPIMP1 TaPIMP1 GmMYB028 GmMYB028 GmMYB110 GmMYB110 AtMYB78 AtMYB78 AtMYB108 AtMYB108 GmMYB014 GmMYB014 GmMYB105 GmMYB105 AtMYB81 AtMYB81 AtMYB104 AtMYB104 AtMYB125 AtMYB125 GmMYB143 GmMYB143 AtMYB97 AtMYB97 AtMYB120 AtMYB120 AtMYB101 AtMYB101 GmMYB075 GmMYB075 GmMYB181 GmMYB181 GmMYB090 GmMYB090 GmMYB109 GmMYB109 AtMYB33 AtMYB33 AtMYB65 AtMYB65 GmMYB055 GmMYB055 GmMYB084 GmMYB084 OsGAMYB OsGAMYB HvGAMYB HvGAMYB LtGAMYB LtGAMYB GmMYB140 GmMYB140 GmMYB151 GmMYB151 GmMYB240 GmMYB240 AmPHAN AmPHAN GmMYB030 GmMYB030 GmMYB154 GmMYB154 TaWRS2 TaWRS2 ZmPHAN ZmPHAN LePHAN LePHAN AtMYB91 AtMYB91 GmMYB049 GmMYB049 GmMYB081 GmMYB081 AtMYB1 AtMYB1 AtMYB25 AtMYB25 AtMYB109 AtMYB109 AtMYB44 AtMYB44 AtMYB77 AtMYB77 GmMYB012 GmMYB012 GmMYB019 GmMYB019 GmMYB011 GmMYB011 AtMYB70 AtMYB70 AtMYB73 AtMYB73 GmMYB041 GmMYB041 GmMYB003 GmMYB003 GmMYB032 GmMYB032 GmMYB036 GmMYB036 GmMYB231 GmMYB231 GmMYB068 GmMYB068 GmMYB087 GmMYB087 AtMYB52 AtMYB52 PttMYB21a PttMYB21a AtMYB54 AtMYB54 GmMYB056 GmMYB056 GmMYB060 GmMYB060 AtMYB69 AtMYB69 AtMYB89 AtMYB89 AtMYB110 AtMYB110 GmMYB230 GmMYB230 GmMYB236 GmMYB236 AtMYB56 AtMYB56 GmMYB228 GmMYB228 AtMYB105 AtMYB105 AtMYB117 AtMYB117 GmMYB229 GmMYB229 GmMYB235 GmMYB235 GmMYB088 GmMYB088 AtMYB64 AtMYB64 AtMYB119 AtMYB119 AtMYB118 AtMYB118 AtMYB98 AtMYB98 GmMYB234 GmMYB234 AtMYB115 AtMYB115 AtMYB22 AtMYB22 AtMYB100 AtMYB100 GmMYB237 GmMYB237 GmMYB3R-1 GmMYB3R-1 AtMYB3R3 AtMYB3R3 AtMYB3R5 AtMYB3R5 GmMYB3R-3 GmMYB3R-3 GmMYB3R-2 GmMYB3R-2 ATMYB3R2 ATMYB3R2 GmMYB3R-4 GmMYB3R-4 AtMYB3R1 AtMYB3R1 AtMYB3R4 AtMYB3R4 GmMYB3R-5 GmMYB3R-5 GmMYB3R-6 GmMYB3R-6 AtMYB88 AtMYB88 AtMYB124 AtMYB124 GmMYB051 GmMYB051 GmMYB138 GmMYB138 GmMYB067 GmMYB067 GmMYB091 GmMYB091 AtMYB3R-like AtMYB3R-like GmMYB147 GmMYB147 GmMYB156 GmMYB156 AtCDC5 AtCDC5 AtMYB4R1 AtMYB4R1 GmMYB4R-1 GmMYB4R-1 GmMYB4R-2 GmMYB4R-2

D

Expreesion pattern pattern Expreesion R L L FF SS N N R

0

2

4

6

Additional file 3. The complete version of Figure 2: Phylogenetic relationships and subgroup designations in MYB proteins from soybean (Gm), Arabidopsis (At) and other plants. (a) Neighbor-joining tree representing relationships among 252 MYB proteins from soybean and 132 MYB proteins from Arabidopsis, including five 3R-MYB proteins from Arabidopsis and six 3R-MYB proteins from soybean. The proteins are clustered into 47 subgroups, which are designated with a subgroup number (e.g., C1) and marked with different alternating tones of a gray background to facilitate subfamily identification with high predictive value. The numbers beside the branches represent bootstrap support values (>50%) from 1000 replications. Sixteen proteins did not fit well into clusters. Colored circles indicate the corresponding intron distribution patterns, as shown in Figure 3. (b) Structure of MYB genes in soybean and Arabidopsis. Exon(s) are indicated by green boxes, MYB domain(s) by red boxes, untranslated region(s) by blue boxes, and spaces between the colored boxes correspond to introns. The sizes of exons and introns can be estimated using the horizontal scale bar. (c) Architecture of conserved protein motifs in 47 subfamilies. The motifs on the right were detected using MEME and are graphically represented as white boxes drawn to scale for a representative plant MYB protein of each subfamily. (d) Expression patterns of MYB genes in soybean and Arabidopsis in different organs. R, root; L, leaf; F, flower; S, seed; N, legume-specific nodulation. In this expression pattern analysis, the highest values among the expression values of the four organs published in the AtGenExpress and SoySeq databases were selected.