1st Ca2+ dissociation from CaM N-terminus ... Equilibrium binding of 1st Ca2+ to CaM C- .... Equilibrium binding of CaM1N1C to AC8 N- terminus. 14.8 Β΅M. [4]Β ...
Parameter Description 2+ Ca binding to CaM 1π 1st Ca2+ binding to CaM N-terminus πππ 1π 1st Ca2+ dissociation from CaM N-terminus ππππ Equilibrium binding of 1st Ca2+ to CaM NπΎπ·1π terminus 2π 2nd Ca2+ binding to CaM N-terminus πππ 2π 2nd Ca2+ dissociation from CaM N-terminus ππππ Equilibrium binding of 2nd Ca2+ to CaM NπΎπ·2π terminus 1πΆ 1st Ca2+ binding to CaM C-terminus πππ 1πΆ 1st Ca2+ dissociation from CaM C-terminus ππππ Equilibrium binding of 1st Ca2+ to CaM CπΎπ·1πΆ terminus 2πΆ 2nd Ca2+ binding to CaM C-terminus πππ 2πΆ 2nd Ca2+ dissociation from CaM C-terminus ππππ Equilibrium binding of 2nd Ca2+ to CaM CπΎπ·2πΆ terminus CaM binding to AC1 π΄πΆ1πΆππ0 CaM0 binding to AC1 πππ π΄πΆ1πΆππ0 CaM0 dissociation from AC1 ππππ π΄πΆ1πΆππ0 Equilibrium binding of CaM0 to AC1 πΎπ· π΄πΆ1πΆππ1π CaM1N binding to AC1 πππ π΄πΆ1πΆππ1π CaM1N dissociation from AC1 ππππ Equilibrium binding of CaM1N to AC1 πΎπ·π΄πΆ1πΆππ1π π΄πΆ1πΆππ1πΆ CaM1C binding to AC1 πππ π΄πΆ1πΆππ1πΆ CaM1C dissociation from AC1 ππππ Equilibrium binding of CaM1C to AC1 πΎπ·π΄πΆ1πΆππ1πΆ π΄πΆ1πΆππ1π1πΆ CaM1N1C binding to AC1 πππ π΄πΆ1πΆππ1π1πΆ CaM1N1C dissociation from AC1 ππππ πΎπ·π΄πΆ1πΆππ1π1πΆ Equilibrium binding of CaM1N1C to AC1 π΄πΆ1πΆππ2π CaM2N binding to AC1 πππ π΄πΆ1πΆππ2π CaM2N dissociation from AC1 ππππ Equilibrium binding of CaM2N to AC1 πΎπ·π΄πΆ1πΆππ2π π΄πΆ1πΆππ2πΆ CaM2C binding to AC1 πππ π΄πΆ1πΆππ2πΆ CaM2C dissociation from AC1 ππππ Equilibrium binding of CaM2C to AC1 πΎπ·π΄πΆ1πΆππ2πΆ π΄πΆ1πΆππ2π1πΆ CaM2N1C binding to AC1 πππ π΄πΆ1πΆππ2π1πΆ CaM2N1C dissociation from AC1 ππππ π΄πΆ1πΆππ2π1πΆ Equilibrium binding of CaM2N1C to AC1 πΎπ· π΄πΆ1πΆππ1π2πΆ CaM1N2C binding to AC1 πππ
Value Range
Value Used
Reference(s)
25.0-260.0 ΞΌM-1s-1 1000.0-4000.0 s-1
100.0 ΞΌM-1s-1 2500.0 s-1
15.0-40.0 ΞΌM
25.0 ΞΌM
[1] [1] [1]
50.0-300.0 ΞΌM-1s-1 500.0-1000.0+ s-1
150.0 ΞΌM-1s-1 750.0 s-1
3.3-9.0 ΞΌM
5.0 ΞΌM
1.2-9.6 ΞΌM-1s-1 10.0-70.0 s-1
4.0 ΞΌM-1s-1 40.0 s-1
7.3-12.0 ΞΌM
10.0 ΞΌM
5.0-25.0 ΞΌM-1s-1 8.5-10.0 s-1
10.0 ΞΌM-1s-1 9.25 s-1
0.4-1.7 ΞΌM
0.925 ΞΌM
0.00166 ΞΌM-1s-1 0.9 s-1 542.0 ΞΌM 0.00166 ΞΌM-1s-1 0.9 s-1 542.0 ΞΌM 0.00830 ΞΌM-1s-1 0.9 s-1 108.0 ΞΌM 0.00830 ΞΌM-1s-1 0.9 s-1 108.0 ΞΌM 0.156 ΞΌM-1s-1 0.9 s-1 5.78 ΞΌM 0.064 ΞΌM-1s-1 0.9 s-1 14.1 ΞΌM 0.778 ΞΌM-1s-1 0.9 s-1 1.16 ΞΌM 0.064 ΞΌM-1s-1
[1] [1] [1] [1] [1] [1] [1] [1] [1]
[2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2]
CaM1N2C dissociation from AC1 Equilibrium binding of CaM1N2C to AC1 CaM4 binding to AC1 π΄πΆ1πΆππ4 CaM4 dissociation from AC1 ππππ Equilibrium binding of CaM4 to AC1 πΎπ·π΄πΆ1πΆππ4 Ca2+ binding to AC1-CaM π΄πΆ11π 1st Ca2+ binding to AC1-CaM N-terminus πππ π΄πΆ11π 1st Ca2+ dissociation from AC1-CaM Nππππ terminus Equilibrium binding of 1st Ca2+ to AC1-CaM πΎπ·π΄πΆ11π N-terminus π΄πΆ12π 2nd Ca2+ binding to AC1-CaM N-terminus πππ π΄πΆ12π 2nd Ca2+ dissociation from AC1-CaM Nππππ terminus Equilibrium binding of 2nd Ca2+ to AC1-CaM πΎπ·π΄πΆ12π N-terminus π΄πΆ11πΆ 1st Ca2+ binding to AC1-CaM C-terminus πππ π΄πΆ11πΆ 1st Ca2+ dissociation from AC1-CaM Cππππ terminus π΄πΆ11πΆ Equilibrium binding of 1st Ca2+ to AC1-CaM CπΎπ· terminus π΄πΆ12πΆ 2nd Ca2+ binding to AC1-CaM C-terminus πππ π΄πΆ12πΆ 2nd Ca2+ dissociation from AC1-CaM Cππππ terminus π΄πΆ12πΆ Equilibrium binding of 2nd Ca2+ to AC1-CaM πΎπ· C-terminus CaM binding to AC8 N-terminus π΄πΆ8ππ‘πΆππ0 CaM0 binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ0 CaM0 dissociation from AC8 N-terminus ππππ Equilibrium binding of CaM0 to AC8 NπΎπ·π΄πΆ8ππ‘πΆππ0 terminus π΄πΆ8ππ‘πΆππ1π CaM1N binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ1π CaM1N dissociation from AC8 N-terminus ππππ π΄πΆ8ππ‘πΆππ1π Equilibrium binding of CaM1N to AC8 NπΎπ· terminus π΄πΆ8ππ‘πΆππ1πΆ CaM1C binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ1πΆ CaM1C dissociation from AC8 N-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ1πΆ Equilibrium binding of CaM1C to AC8 Nterminus π΄πΆ8ππ‘πΆππ1π1πΆ CaM1N1C binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ1π1πΆ CaM1N1C dissociation from AC8 N-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ1π1πΆ Equilibrium binding of CaM1N1C to AC8 Nterminus π΄πΆ1πΆππ1π2πΆ ππππ πΎπ·π΄πΆ1πΆππ1π2πΆ π΄πΆ1πΆππ4 πππ
0.9 s-1
0.15 ΞΌM
[3] [4] [5] [5] [2]
100.0 ΞΌM-1s-1 2500.0 s-1
[6] [8]
25.0 ΞΌM
[2]
150.0 ΞΌM-1s-1 8.0 s-1
[6] [8]
0.0533 ΞΌM
[2]
4.0 ΞΌM-1s-1 8.0 s-1
[6] [8]
2.0 ΞΌM
[2]
10.0 ΞΌM-1s-1 1.2 s-1
[6] [8]
0.12 ΞΌM
[2]
0.00828 ΞΌM-1s-1 1.0 s-1
[2] [3] [4]
14.1 ΞΌM 6.0 ΞΌM-1s-1 0.9 s-1
121.0 ΞΌM 0.00828 ΞΌM-1s-1 1.0 s-1 121.0 ΞΌM 0.0676 ΞΌM-1s-1 1.0 s-1 14.8 ΞΌM 0.0676 ΞΌM-1s-1 1.0 s-1 14.8 ΞΌM
[2] [3] [4] [2] [3] [4] [2] [3] [4]
CaM2N binding to AC8 N-terminus π΄πΆ8ππ‘πΆππ2π CaM2N dissociation from AC8 N-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ2π Equilibrium binding of CaM2N to AC8 Nterminus π΄πΆ8ππ‘πΆππ2πΆ CaM2C binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ2πΆ CaM2C dissociation from AC8 N-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ2πΆ Equilibrium binding of CaM2C to AC8 Nterminus π΄πΆ8ππ‘πΆππ2π1πΆ CaM2N1C binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ2π1πΆ CaM2N1C dissociation from AC8 N-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ2π1πΆ Equilibrium binding of CaM2N1C to AC8 Nterminus π΄πΆ8ππ‘πΆππ1π2πΆ CaM1N2C binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ1π2πΆ CaM1N2C dissociation from AC8 N-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ1π2πΆ Equilibrium binding of CaM1N2C to AC8 Nterminus π΄πΆ8ππ‘πΆππ4 CaM4 binding to AC8 N-terminus πππ π΄πΆ8ππ‘πΆππ4 CaM4 dissociation from AC8 N-terminus ππππ π΄πΆ8ππ‘πΆππ4 Equilibrium binding of CaM4 to AC8 NπΎπ· terminus 2+ Ca binding to AC8(N-terminus)-CaM π΄πΆ8ππ‘1π 1st Ca2+ binding to AC8(N-terminus)-CaM Nπππ terminus π΄πΆ8ππ‘1π 1st Ca2+ dissociation from AC8(N-terminus)ππππ CaM N-terminus π΄πΆ8ππ‘1π Equilibrium binding of 1st Ca2+ to AC8(NπΎπ· terminus)-CaM N-terminus π΄πΆ8ππ‘2π 2nd Ca2+ binding to AC8(N-terminus)-CaM Nπππ terminus π΄πΆ8ππ‘2π 2nd Ca2+ dissociation from AC8(N-terminus)ππππ CaM N-terminus Equilibrium binding of 2nd Ca2+ to AC8(NπΎπ·π΄πΆ8ππ‘2π terminus)-CaM N-terminus π΄πΆ8ππ‘1πΆ 1st Ca2+ binding to AC8(N-terminus)-CaM Cπππ terminus π΄πΆ8ππ‘1πΆ 1st Ca2+ dissociation from AC8(N-terminus)ππππ CaM C-terminus Equilibrium binding of 1st Ca2+ to AC8(NπΎπ·π΄πΆ8ππ‘1πΆ terminus)-CaM C-terminus π΄πΆ8ππ‘2πΆ 2nd Ca2+ binding to AC8(N-terminus)-CaM Cπππ terminus π΄πΆ8ππ‘2πΆ 2nd Ca2+ dissociation from AC8(N-terminus)ππππ CaM C-terminus π΄πΆ8ππ‘πΆππ2π πππ
0.00828 ΞΌM-1s-1 1.0 s-1 121.0 ΞΌM 1.25 ΞΌM-1s-1 1.0 s-1 0.8 ΞΌM 0.0676 ΞΌM-1s-1 1.0 s-1 14.8 ΞΌM 1.25 ΞΌM-1s-1 1.0 s-1 0.8 ΞΌM
[2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4]
0.8 ΞΌM
[5] [5] [2]
100.0 ΞΌM-1s-1
[6]
2500.0 s-1
[7]
25.0 ΞΌM
[2]
150.0 ΞΌM-1s-1
[6]
750.0 s-1
[8]
5.0 ΞΌM
[2]
4.0 ΞΌM-1s-1
[6]
4.9 s-1
[8]
1.23 ΞΌM
[2]
10.0 ΞΌM-1s-1
[6]
0.5 s-1
[8]
1.25 ΞΌM-1s-1 1.0 s-1
Equilibrium binding of 2nd Ca2+ to AC8(Nterminus)-CaM C-terminus CaM binding to AC8 C-terminus π΄πΆ8ππ‘πΆππ0 CaM0 binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ0 CaM0 dissociation from AC8 C-terminus ππππ Equilibrium binding of CaM0 to AC8 CπΎπ·π΄πΆ8ππ‘πΆππ0 terminus π΄πΆ8ππ‘πΆππ1π CaM1N binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ1π CaM1N dissociation from AC8 C-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ1π Equilibrium binding of CaM1N to AC8 Cterminus π΄πΆ8ππ‘πΆππ1πΆ CaM1C binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ1πΆ CaM1C dissociation from AC8 C-terminus ππππ π΄πΆ8ππ‘πΆππ1πΆ Equilibrium binding of CaM1C to AC8 CπΎπ· terminus π΄πΆ8ππ‘πΆππ1π1πΆ CaM1N1C binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ1π1πΆ CaM1N1C dissociation from AC8 C-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ1π1πΆ Equilibrium binding of CaM1N1C to AC8 Cterminus π΄πΆ8ππ‘πΆππ2π CaM2N binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ2π CaM2N dissociation from AC8 C-terminus ππππ π΄πΆ8ππ‘πΆππ2π Equilibrium binding of CaM2N to AC8 CπΎπ· terminus π΄πΆ8ππ‘πΆππ2πΆ CaM2C binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ2πΆ CaM2C dissociation from AC8 C-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ2πΆ Equilibrium binding of CaM2C to AC8 Cterminus π΄πΆ8ππ‘πΆππ2π1πΆ CaM2N1C binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ2π1πΆ CaM2N1C dissociation from AC8 C-terminus ππππ πΎπ·π΄πΆ8ππ‘πΆππ2π1πΆ Equilibrium binding of CaM2N1C to AC8 Cterminus π΄πΆ8ππ‘πΆππ1π2πΆ CaM1N2C binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ1π2πΆ CaM1N2C dissociation from AC8 C-terminus ππππ π΄πΆ8ππ‘πΆππ1π2πΆ Equilibrium binding of CaM1N2C to AC8 CπΎπ· terminus π΄πΆ8ππ‘πΆππ4 CaM4 binding to AC8 C-terminus πππ π΄πΆ8ππ‘πΆππ4 CaM4 dissociation from AC8 C-terminus ππππ Equilibrium binding of CaM4 to AC8 CπΎπ·π΄πΆ8ππ‘πΆππ4 terminus Ca2+ binding to AC8(C-terminus)-CaM π΄πΆ8ππ‘1π 1st Ca2+ binding to AC8(C-terminus)-CaM Nπππ terminus πΎπ·π΄πΆ8ππ‘2πΆ
0.05 ΞΌM
[2]
0.00267 ΞΌM-1s-1 1.0 s-1
[2] [3] [4]
375.0 ΞΌM 0.00267 ΞΌM-1s-1 1.0 s-1 375.0 ΞΌM 0.00267 ΞΌM-1s-1 1.0 s-1 375.0 ΞΌM 0.00267 ΞΌM-1s-1 1.0 s-1 375.0 ΞΌM 1.25 ΞΌM-1s-1 1.0 s-1 0.8 ΞΌM 0.00267 ΞΌM-1s-1 1.0 s-1 375.0 ΞΌM 1.25 ΞΌM-1s-1 1.0 s-1 0.8 ΞΌM 0.00267 ΞΌM-1s-1 1.0 s-1 375.0 ΞΌM
[2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4]
0.8 ΞΌM
[5] [5] [2]
100.0 ΞΌM-1s-1
[6]
1.25 ΞΌM-1s-1 1.0 s-1
π΄πΆ8ππ‘1π ππππ
πΎπ·π΄πΆ8ππ‘1π π΄πΆ8ππ‘2π πππ π΄πΆ8ππ‘2π ππππ
πΎπ·π΄πΆ8ππ‘2π π΄πΆ8ππ‘1πΆ πππ π΄πΆ8ππ‘1πΆ ππππ
πΎπ·π΄πΆ8ππ‘1πΆ π΄πΆ8ππ‘2πΆ πππ π΄πΆ8ππ‘2πΆ ππππ
πΎπ·π΄πΆ8ππ‘2πΆ
1st Ca2+ dissociation from AC8(C-terminus)CaM N-terminus Equilibrium binding of 1st Ca2+ to AC8(Cterminus)-CaM N-terminus 2nd Ca2+ binding to AC8(C-terminus)-CaM Nterminus 2nd Ca2+ dissociation from AC8(C-terminus)CaM N-terminus Equilibrium binding of 2nd Ca2+ to AC8(Cterminus)-CaM N-terminus 1st Ca2+ binding to AC8(C-terminus)-CaM Cterminus 1st Ca2+ dissociation from AC8(C-terminus)CaM C-terminus Equilibrium binding of 1st Ca2+ to AC8(Cterminus)-CaM C-terminus 2nd Ca2+ binding to AC8(C-terminus)-CaM Cterminus 2nd Ca2+ dissociation from AC8(C-terminus)CaM C-terminus Equilibrium binding of 2nd Ca2+ to AC8(Cterminus)-CaM C-terminus
CaM binding to CaN πππΆππ0 CaM0 binding to CaN πππ πππΆππ0 CaM0 dissociation from CaN ππππ Equilibrium binding of CaM0 to CaN πΎπ·πππΆππ0 πππΆππ1π CaM1N binding to CaN πππ πππΆππ1π CaM1N dissociation from CaN ππππ Equilibrium binding of CaM1N to CaN πΎπ·πππΆππ1π πππΆππ1πΆ CaM1C binding to CaN πππ πππΆππ1πΆ CaM1C dissociation from CaN ππππ πππΆππ1πΆ Equilibrium binding of CaM1C to CaN πΎπ· πππΆππ1π1πΆ CaM1N1C binding to CaN πππ πππΆππ1π1πΆ CaM1N1C dissociation from CaN ππππ πππΆππ1π1πΆ Equilibrium binding of CaM1N1C to CaN πΎπ· πππΆππ2π CaM2N binding to CaN πππ πππΆππ2π CaM2N dissociation from CaN ππππ πππΆππ2π Equilibrium binding of CaM2N to CaN πΎπ· πππΆππ2πΆ CaM2C binding to CaN πππ πππΆππ2πΆ CaM2C dissociation from CaN ππππ Equilibrium binding of CaM2C to CaN πΎπ·πππΆππ2πΆ πππΆππ2π1πΆ CaM2N1C binding to CaN πππ πππΆππ2π1πΆ CaM2N1C dissociation from CaN ππππ
2500.0 s-1
[7]
25.0 ΞΌM
[2]
150.0 ΞΌM-1s-1
[6]
1.6 s-1
[8]
0.0107 ΞΌM
[2]
4.0 ΞΌM-1s-1
[6]
40.0 s-1
[8]
10.0 ΞΌM
[2]
10.0 ΞΌM-1s-1
[6]
9.25 s-1
[8]
0.925 ΞΌM
[2]
0.0000000798 ΞΌM-1s-1 0.000319 s-1
[2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3]
3999.0 ΞΌM 0.00000665 ΞΌM-1s-1 0.000319 s-1 48.0 ΞΌM 0.00001334 ΞΌM-1s-1 0.000319 s-1 23.9 ΞΌM 0.000665 ΞΌM-1s-1 0.000319 s-1 0.480 ΞΌM 0.000416 ΞΌM-1s-1 0.000319 s-1 0.768 ΞΌM 0.000123 ΞΌM-1s-1 0.000319 s-1 2.59 ΞΌM 0.0416 ΞΌM-1s-1 0.000319 s-1
Equilibrium binding of CaM2N1C to CaN CaM1N2C binding to CaN πππΆππ1π2πΆ CaM1N2C dissociation from CaN ππππ Equilibrium binding of CaM1N2C to CaN πΎπ·πππΆππ1π2πΆ πππΆππ4 CaM4 binding to CaN 0.0089-46.0 ΞΌM-1s-1 πππ πππΆππ4 CaM4 dissociation from CaN 0.000085-0.0039 s-1 ππππ Equilibrium binding of CaM4 to CaN 0.000028-0.024 ΞΌM πΎπ·πππΆππ4 2+ Ca binding to CaN-CaM ππ1π 1st Ca2+ binding to CaN-CaM N-terminus πππ ππ1π 1st Ca2+ dissociation from CaN-CaM Nππππ terminus ππ1π Equilibrium binding of 1st Ca2+ to CaN-CaM NπΎπ· terminus ππ2π 2nd Ca2+ binding to CaN-CaM N-terminus πππ ππ2π 2nd Ca2+ dissociation from CaN-CaM Nππππ terminus Equilibrium binding of 2nd Ca2+ to CaN-CaM πΎπ·ππ2π N-terminus ππ1πΆ 1st Ca2+ binding to CaN-CaM C-terminus πππ ππ1πΆ 1st Ca2+ dissociation from CaN-CaM Cππππ terminus ππ1πΆ Equilibrium binding of 1st Ca2+ to CaN-CaM CπΎπ· terminus ππ2πΆ 2nd Ca2+ binding to CaN-CaM C-terminus πππ ππ2πΆ 2nd Ca2+ dissociation from CaN-CaM Cππππ terminus ππ2πΆ Equilibrium binding of 2nd Ca2+ to CaN-CaM πΎπ· C-terminus CaM binding to CaMKII πΎπΆππ0 CaM0 binding to CaMKII πππ πΎπΆππ0 CaM0 dissociation from CaMKII ππππ Equilibrium binding of CaM0 to CaMKII πΎπ·πΎπΆππ0 πΎπΆππ1π CaM1N binding to CaMKII πππ πΎπΆππ1π CaM1N dissociation from CaMKII ππππ Equilibrium binding of CaM1N to CaMKII πΎπ·πΎπΆππ1π πΎπΆππ1πΆ CaM1C binding to CaMKII πππ πΎπΆππ1πΆ CaM1C dissociation from CaMKII ππππ Equilibrium binding of CaM1C to CaMKII πΎπ·πΎπΆππ1πΆ πΎπΆππ1π1πΆ CaM1N1C binding to CaMKII πππ πΎπΆππ1π1πΆ CaM1N1C dissociation from CaMKII ππππ Equilibrium binding of CaM1N1C to CaMKII πΎπ·πΎπΆππ1π1πΆ πΎπΆππ2π CaM2N binding to CaMKII πππ πΎπ·πππΆππ2π1πΆ πππΆππ1π2πΆ πππ
0.000498 ΞΌM
[4] [2] [3] [4] [9-11] [9-11] [9-11]
100.0 ΞΌM-1s-1 30.0 s-1
[6] [2]
0.3 ΞΌM
[12]
150.0 ΞΌM-1s-1 12.0 s-1
[6] [2]
0.08 ΞΌM
[12]
4.0 ΞΌM-1s-1 0.4 s-1
[6] [2]
0.1 ΞΌM
[12]
10.0 ΞΌM-1s-1 0.6 s-1
[6] [2]
0.06 ΞΌM
[12]
0.0038 ΞΌM-1s-1 5.5 s-1
[1] [1] [2] [1] [1] [2] [1] [1] [2] [1] [1] [2] [1]
0.00768 ΞΌM 0.0102 ΞΌM-1s-1 0.000319 s-1 0.0311 ΞΌM 0.64 ΞΌM-1s-1 0.000319 s-1
1.45 mM 0.022 ΞΌM-1s-1 3.1 s-1 141.0 ΞΌM 0.059 ΞΌM-1s-1 6.1 s-1 103.0 ΞΌM 0.33 ΞΌM-1s-1 3.4 s-1 10.3 ΞΌM 0.12 ΞΌM-1s-1
CaM2N dissociation from CaMKII Equilibrium binding of CaM2N to CaMKII CaM2C binding to CaMKII πΎπΆππ2πΆ CaM2C dissociation from CaMKII ππππ Equilibrium binding of CaM2C to CaMKII πΎπ·πΎπΆππ2πΆ πΎπΆππ2π1πΆ CaM2N1C binding to CaMKII πππ πΎπΆππ2π1πΆ CaM2N1C dissociation from CaMKII ππππ Equilibrium binding of CaM2N1C to CaMKII πΎπ·πΎπΆππ2π1πΆ πΎπΆππ1π2πΆ CaM1N2C binding to CaMKII πππ πΎπΆππ1π2πΆ CaM1N2C dissociation from CaMKII ππππ Equilibrium binding of CaM1N2C to CaMKII πΎπ·πΎπΆππ1π2πΆ πΎπΆππ4 CaM4 binding to CaMKII πππ πΎπΆππ4 CaM4 dissociation from CaMKII ππππ πΎπΆππ4 Equilibrium binding of CaM4 to CaMKII πΎπ· Ca2+ binding to CaMKII-CaM πΎ1π 1st Ca2+ binding to CaMKII-CaM N-terminus πππ πΎ1π 1st Ca2+ dissociation from CaMKII-CaM Nππππ terminus πΎ1π Equilibrium binding of 1st Ca2+ to CaMKIIπΎπ· CaM N-terminus πΎ2π 2nd Ca2+ binding to CaMKII-CaM N-terminus πππ πΎ2π 2nd Ca2+ dissociation from CaMKII-CaM Nππππ terminus πΎ2π Equilibrium binding of 2nd Ca2+ to CaMKIIπΎπ· CaM N-terminus πΎ1πΆ 1st Ca2+ binding to CaMKII-CaM C-terminus πππ πΎ1πΆ 1st Ca2+ dissociation from CaMKII-CaM Cππππ terminus πΎ1πΆ Equilibrium binding of 1st Ca2+ to CaMKIIπΎπ· CaM C-terminus πΎ2πΆ 2nd Ca2+ binding to CaMKII-CaM C-terminus πππ πΎ2πΆ 2nd Ca2+ dissociation from CaMKII-CaM Cππππ terminus Equilibrium binding of 2nd Ca2+ to CaMKIIπΎπ·πΎ2πΆ CaM C-terminus CaM binding to MLCK ππΎπΆππ0 CaM0 binding to MLCK πππ ππΎπΆππ0 CaM0 dissociation from MLCK ππππ ππΎπΆππ0 Equilibrium binding of CaM0 to MLCK πΎπ· ππΎπΆππ1π CaM1N binding to MLCK πππ ππΎπΆππ1π CaM1N dissociation from MLCK ππππ ππΎπΆππ1π Equilibrium binding of CaM1N to MLCK πΎπ· πΎπΆππ2π ππππ πΎπ·πΎπΆππ2π πΎπΆππ2πΆ πππ
1.7 s-1
14.0-60.0 ΞΌM-1s-1 1.1-2.3 s-1
0.731 ΞΌM 30.0 ΞΌM-1s-1 1.7 s-1
0.04-0.08 ΞΌM
0.0567 ΞΌM
[1] [1] [1] [1] [1] [1] [1] [2] [1] [1] [2] [1] [1] [1]
76.0 ΞΌM-1s-1 300.0 s-1
[1] [1]
3.95 ΞΌM
[2]
76.0 ΞΌM-1s-1 29.6 s-1
[1] [1]
0.39 ΞΌM
[2]
44.0 ΞΌM-1s-1 33.0 s-1
[1] [1]
0.75 ΞΌM
[2]
44.0 ΞΌM-1s-1 2.7 s-1
[1] [1]
0.0614 ΞΌM
[2]
0.00717 ΞΌM-1s-1 0.132 s-1
[2] [3] [4] [2] [3] [4]
16.5-23.5 ΞΌM
14.2 ΞΌM 0.92 ΞΌM-1s-1 6.8 s-1
1.6-8.4 ΞΌM
7.39 ΞΌM 1.9 ΞΌM-1s-1 1.9 s-1 1.0 ΞΌM 5.2 ΞΌM-1s-1 3.8 s-1
6.0-60.0
s-1
0.49-4.9 s-1
18.4 ΞΌM 0.00717 ΞΌM-1s-1 0.132 s-1 18.4 ΞΌM
CaM1C binding to MLCK ππΎπΆππ1πΆ CaM1C dissociation from MLCK ππππ Equilibrium binding of CaM1C to MLCK πΎπ·ππΎπΆππ1πΆ ππΎπΆππ1π1πΆ CaM1N1C binding to MLCK πππ ππΎπΆππ1π1πΆ CaM1N1C dissociation from MLCK ππππ ππΎπΆππ1π1πΆ Equilibrium binding of CaM1N1C to MLCK πΎπ· ππΎπΆππ2π CaM2N binding to MLCK πππ ππΎπΆππ2π CaM2N dissociation from MLCK ππππ ππΎπΆππ2π Equilibrium binding of CaM2N to MLCK πΎπ· ππΎπΆππ2πΆ CaM2C binding to MLCK πππ ππΎπΆππ2πΆ CaM2C dissociation from MLCK ππππ Equilibrium binding of CaM2C to MLCK πΎπ·ππΎπΆππ2πΆ ππΎπΆππ2π1πΆ CaM2N1C binding to MLCK πππ ππΎπΆππ2π1πΆ CaM2N1C dissociation from MLCK ππππ πΎπ·ππΎπΆππ2π1πΆ Equilibrium binding of CaM2N1C to MLCK ππΎπΆππ1π2πΆ CaM1N2C binding to MLCK πππ ππΎπΆππ1π2πΆ CaM1N2C dissociation from MLCK ππππ πΎπ·ππΎπΆππ1π2πΆ Equilibrium binding of CaM1N2C to MLCK ππΎπΆππ4 CaM4 binding to MLCK πππ ππΎπΆππ4 CaM4 dissociation from MLCK ππππ Equilibrium binding of CaM4 to MLCK πΎπ·ππΎπΆππ4 2+ Ca binding to MLCK-CaM ππΎ1π 1st Ca2+ binding to MLCK-CaM N-terminus πππ ππΎ1π 1st Ca2+ dissociation from MLCK-CaM Nππππ terminus Equilibrium binding of 1st Ca2+ to MLCK-CaM πΎπ·ππΎ1π N-terminus ππΎ2π 2nd Ca2+ binding to MLCK-CaM N-terminus πππ ππΎ2π 2nd Ca2+ dissociation from MLCK-CaM Nππππ terminus ππΎ2π Equilibrium binding of 2nd Ca2+ to MLCK-CaM πΎπ· N-terminus ππΎ1πΆ 1st Ca2+ binding to MLCK-CaM C-terminus πππ ππΎ1πΆ 1st Ca2+ dissociation from MLCK-CaM Cππππ terminus ππΎ1πΆ Equilibrium binding of 1st Ca2+ to MLCK-CaM πΎπ· C-terminus ππΎ2πΆ 2nd Ca2+ binding to MLCK-CaM C-terminus πππ ππΎ2πΆ 2nd Ca2+ dissociation from MLCK-CaM Cππππ terminus ππΎ2πΆ Equilibrium binding of 2nd Ca2+ to MLCK-CaM πΎπ· C-terminus
28.0-110.0 ΞΌM-1s-1 0.031-0.56 s-1
0.776 ΞΌM 55.5 ΞΌM-1s-1 0.132 s-1
0.0011-0.22 ΞΌM
0.00238 ΞΌM
[2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [13-15] [13-15] [13-15]
100.0 ΞΌM-1s-1 2500.0 s-1
[6] [7]
25.0 ΞΌM
[2]
150.0 ΞΌM-1s-1 2.3 s-1
[6] [15-18]
0.015 ΞΌM
[2]
4.0 ΞΌM-1s-1 40.0 s-1
[6] [7]
10.0 ΞΌM
[2]
10.0 ΞΌM-1s-1 0.39 s-1
[6] [15-18]
0.039 ΞΌM
[2]
ππΎπΆππ1πΆ πππ
0.00717 ΞΌM-1s-1 0.132 s-1 18.4 ΞΌM 0.00717 ΞΌM-1s-1 0.132 s-1 18.4 ΞΌM 2.34 ΞΌM-1s-1 0.132 s-1 0.0564 ΞΌM 0.170 ΞΌM-1s-1 0.132 s-1 0.776 ΞΌM 2.34 ΞΌM-1s-1 0.132 s-1 0.0564 ΞΌM 0.170 ΞΌM-1s-1 0.132 s-1
2.3-12.5 s-1
0.39-0.7
s-1
CaM binding to Ng πππΆππ0
CaM0 binding to Ng CaM0 dissociation from Ng
28.0 ΞΌM-1s-1 36.0 s-1
[19] [19]
πππΆππ0
Equilibrium binding of CaM0 to Ng CaM1N binding to Ng CaM1N dissociation from Ng
1.29 ΞΌM 28.0 ΞΌM-1s-1 36.0 s-1
[19] [19] [19]
πΎπ· πππΆππ1πΆ πππ πππΆππ1πΆ ππππ
πππΆππ1π
Equilibrium binding of CaM1N to Ng CaM1C binding to Ng CaM1C dissociation from Ng
1.29 ΞΌM 23.0 ΞΌM-1s-1 35.0 s-1
[19] [19] [19]
πππΆππ1πΆ
Equilibrium binding of CaM1C to Ng CaM1N1C binding to Ng CaM1N1C dissociation from Ng
1.52 ΞΌM 23.0 ΞΌM-1s-1 35.0 s-1
[19] [19] [19]
Equilibrium binding of CaM1N1C to Ng CaM2N binding to Ng CaM2N dissociation from Ng
1.52 ΞΌM 28.0 ΞΌM-1s-1 36.0 s-1
[19] [19] [19]
πΎπ· πππΆππ2πΆ πππ πππΆππ2πΆ ππππ
πππΆππ2π
Equilibrium binding of CaM2N to Ng CaM2C binding to Ng CaM2C dissociation from Ng
1.29 ΞΌM 2.0 ΞΌM-1s-1 136.0 s-1
[19] [19] [19]
πππΆππ2πΆ
Equilibrium binding of CaM2C to Ng CaM2N1C binding to Ng CaM2N1C dissociation from Ng
68.0 ΞΌM 23.0 ΞΌM-1s-1 35.0 s-1
[19] [19] [19]
πΎπ· πππΆππ1π2πΆ πππ πππΆππ1π2πΆ ππππ
πππΆππ2π1πΆ
Equilibrium binding of CaM2N1C to Ng CaM1N2C binding to Ng CaM1N2C dissociation from Ng
1.52 ΞΌM 2.0 ΞΌM-1s-1 136.0 s-1
[19] [19] [19]
πππΆππ1π2πΆ
Equilibrium binding of CaM1N2C to Ng CaM4 binding to Ng CaM4 dissociation from Ng
68.0 ΞΌM 2.0 ΞΌM-1s-1 136.0 s-1
[19] [19] [19]
πππΆππ4 Equilibrium binding of CaM4 to Ng πΎπ· 2+ Ca binding to Ng-CaM ππ1π 1st Ca2+ binding to Ng-CaM N-terminus πππ ππ1π 1st C a2+ dissociation from Ng-CaM N-terminus π
68.0 ΞΌM
[19]
100.0 ΞΌM-1s-1 2500.0 s-1
[19] [19]
Equilibrium binding of 1st Ca2+ to Ng-CaM Nterminus 2nd Ca2+ binding to Ng-CaM N-terminus 2nd Ca2+ dissociation from Ng-CaM N-terminus
25.0 ΞΌM
[19]
150.0 ΞΌM-1s-1 750.0 s-1
[19] [19]
Equilibrium binding of 2nd Ca2+ to Ng-CaM Nterminus 1st Ca2+ binding to Ng-CaM C-terminus 1st Ca2+ dissociation from Ng-CaM C-terminus
5.0 ΞΌM
[19]
426.0 ΞΌM-1s-1 5830.0 s-1
[19] [19]
πππ πππΆππ0 ππππ πΎπ· πππΆππ1π πππ πππΆππ1π ππππ
πΎπ· πππΆππ1π1πΆ πππ πππΆππ1π1πΆ ππππ πππΆππ1π1πΆ
πΎπ· πππΆππ2π πππ πππΆππ2π ππππ
πΎπ· πππΆππ2π1πΆ πππ πππΆππ2π1πΆ ππππ
πΎπ· πππΆππ4 πππ πππΆππ4 ππππ
πππ ππ1π πΎπ· ππ2π
πππ ππ2π ππππ ππ2π
πΎπ·
ππ1πΆ
πππ ππ1πΆ ππππ
ππ1πΆ
πΎπ·
ππ2πΆ
πππ ππ2πΆ ππππ ππ2πΆ
πΎπ·
Equilibrium binding of 1st Ca2+ to Ng-CaM Cterminus 2nd Ca2+ binding to Ng-CaM C-terminus 2nd Ca2+ dissociation from Ng-CaM C-terminus
13.7 ΞΌM
[19]
21.5 ΞΌM-1s-1 418.0 s-1
[19] [19]
Equilibrium binding of 2nd Ca2+ to Ng-CaM Cterminus
19.4 ΞΌM
[19]
0.135 ΞΌM-1s-1 0.01 s-1
CaM binding to NOS ππππΆππ0 CaM0 binding to NOS πππ ππππΆππ0 CaM0 dissociation from NOS ππππ ππππΆππ0 Equilibrium binding of CaM0 to NOS πΎπ· ππππΆππ1π CaM1N binding to NOS πππ ππππΆππ1π CaM1N dissociation from NOS ππππ ππππΆππ1π Equilibrium binding of CaM1N to NOS πΎπ· ππππΆππ1πΆ CaM1C binding to NOS πππ ππππΆππ1πΆ CaM1C dissociation from NOS ππππ ππππΆππ1πΆ Equilibrium binding of CaM1C to NOS πΎπ· ππππΆππ1π1πΆ CaM1N1C binding to NOS πππ ππππΆππ1π1πΆ CaM1N1C dissociation from NOS ππππ πΎπ·ππππΆππ1π1πΆ Equilibrium binding of CaM1N1C to NOS ππππΆππ2π CaM2N binding to NOS πππ ππππΆππ2π CaM2N dissociation from NOS ππππ Equilibrium binding of CaM2N to NOS πΎπ·ππππΆππ2π ππππΆππ2πΆ CaM2C binding to NOS πππ ππππΆππ2πΆ CaM2C dissociation from NOS ππππ Equilibrium binding of CaM2C to NOS πΎπ·ππππΆππ2πΆ ππππΆππ2π1πΆ CaM2N1C binding to NOS πππ ππππΆππ2π1πΆ CaM2N1C dissociation from NOS ππππ πΎπ·ππππΆππ2π1πΆ Equilibrium binding of CaM2N1C to NOS ππππΆππ1π2πΆ CaM1N2C binding to NOS πππ ππππΆππ1π2πΆ CaM1N2C dissociation from NOS ππππ πΎπ·ππππΆππ1π2πΆ Equilibrium binding of CaM1N2C to NOS ππππΆππ4 CaM4 binding to NOS πππ ππππΆππ4 CaM4 dissociation from NOS ππππ ππππΆππ4 Equilibrium binding of CaM4 to NOS πΎπ· Ca2+ binding to NOS-CaM πππ1π 1st Ca2+ binding to NOS-CaM N-terminus πππ πππ1π 1st Ca2+ dissociation from NOS-CaM Nππππ terminus πππ1π Equilibrium binding of 1st Ca2+ to NOS-CaM πΎπ· N-terminus πππ2π 2nd Ca2+ binding to NOS-CaM N-terminus πππ
0.11-660.0 ΞΌM-1s-1 0.0023-3.7 s-1
0.008 ΞΌM 1.25 ΞΌM-1s-1 0.01 s-1
0.005-5.6 ΞΌM
0.008 ΞΌM
[2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [2] [3] [4] [20-22] [20-22] [20-24]
100.0 ΞΌM-1s-1 2500.0 s-1
[6] [25]
25.0 ΞΌM
[2]
150.0 ΞΌM-1s-1
[6]
0.074 ΞΌM 0.135 ΞΌM-1s-1 0.01 s-1 0.074 ΞΌM 0.135 ΞΌM-1s-1 0.01 s-1 0.074 ΞΌM 0.135 ΞΌM-1s-1 0.01 s-1 0.074 ΞΌM 0.135 ΞΌM-1s-1 0.01 s-1 0.074 ΞΌM 1.25 ΞΌM-1s-1 0.01 s-1 0.008 ΞΌM 0.135 ΞΌM-1s-1 0.01 s-1 0.074 ΞΌM 1.25 ΞΌM-1s-1 0.01 s-1
πππ2π ππππ
πΎπ·πππ2π πππ1πΆ πππ πππ1πΆ ππππ
πΎπ·πππ1πΆ πππ2πΆ πππ πππ2πΆ ππππ
πΎπ·πππ2πΆ
2nd Ca2+ dissociation from NOS-CaM Nterminus Equilibrium binding of 2nd Ca2+ to NOS-CaM N-terminus 1st Ca2+ binding to NOS-CaM C-terminus 1st Ca2+ dissociation from NOS-CaM Cterminus Equilibrium binding of 1st Ca2+ to NOS-CaM C-terminus 2nd Ca2+ binding to NOS-CaM C-terminus 2nd Ca2+ dissociation from NOS-CaM Cterminus Equilibrium binding of 2nd Ca2+ to NOS-CaM C-terminus
750.0 s-1
[25]
5.0 ΞΌM
[2]
4.0 ΞΌM-1s-1 40.0 s-1
[6] [7]
10.0 ΞΌM
[2]
10.0 ΞΌM-1s-1 1.0 s-1
[6] [25]
0.1 ΞΌM
[2]
References: [1] S. Pepke et al., βA Dynamic Model of Interactions of Ca2+, Calmodulin, and Catalytic Subunits of Ca2+/Calmodulin-Dependent Protein Kinase II,β PLoS Comput. Biol., vol. 6, no. 2, p. e1000675, 2010. [2] Calculated from the relationship πΎπ· =
ππππ πππ
[3] Assume that Ca2+ binding does not affect the rate of protein dissociation from CaM [4] Calculated from the thermodynamic principle of microscopic reversibility [5] M. Kim et al., βColocalization of Protein Kinase A with Adenylyl Cyclase Enhances Protein Kinase A Activity during Long-Lasting LongTerm-Potentiation,β PLoS Comput. Biol., vol. 7, no. 6, p. e1002084, 2011. [6] Assume that protein binding does not affect the rate of Ca2+ association to CaM [7] Assume that protein binding does not affect the fast dissociation rate(s) of Ca2+ from CaM [8] N. Masada et al., βDistinct Mechanisms of Calmodulin Binding and Regulation of Adenylyl Cyclases 1 and 8,β Biochem., vol. 51, no. 40, pp. 7917-7929, 2012. [9] A.R. Quintana et al., βKinetics of calmodulin binding to calcineurin,β BBRC, vol. 334, pp. 674-680, 2005. [10] M.J. Hubbard and C.B. Klee, βCalmodulin Binding by Calcineurin,β J. Biol. Chem., vol. 262, no. 31, pp. 15062-15070, 1987. [11] E. Takano, M. Hatanaka, and M. Maki, βReal-time analysis of the calcium-dependent interaction between calmodulin and a synthetic oligopeptide of calcineurin by a surface plasmon resonance biosensor,β FEBS Letters, vol. 352, pp. 247-250, 1994.* [12] P.M. Stemmer and C.B. Klee, βDual Calcium Ion Regulation of Calcineurin by Calmodulin and Calcineurin B,β Biochem., vol. 33, pp. 6859-6866, 1994. [13] K. Torok and D.R. Trentham, βMechanism of 2-Chloro-(Ξ΅-amino-Lys75)-[6-[4-(N,N-diethylamino)phenyl]-1,3,5-triazin-4-yl]calmodulin Interactions with Smooth Muscle Myosin Light Chain Kinase and Derived Peptides,β Biochem., vol. 33, pp. 12807-12820, 1994.
[14] R. Kasturi, C. Vasulka, and J.D. Johnson, βCa2+, Caldesmon, and Myosin Light Chain Kinase Exchange with Calmodulin,β J. Biol. Chem., vol. 268, pp. 7958-7964, 1993. [15] M.C. Potier et al., βThe Human Myosin Light Chain Kinase (MLCK) from Hippocampus: Cloning, Sequencing, Expression, and Localization to 3qcen-q21,β Genomics, vol. 29, no. 3, pp. 562-570, 1995.β [17] J.D. Johnson et al., βEffects of Myosin Light Chain Kinase and Peptides on Ca2+ Exchange with the N- and C-terminal Ca2+ Binding Sites of Calmodulin,β J. Biol. Chem., pp. 761-767, 1996. [18] O.B. Peersen, T.S. Madsen, and J.J. Falke, βIntermolecular tuning of calmodulin by target peptides and proteins: Differential effects of Ca2+ binding and implications for kinase activation,β Protein Sci., vol. 6, pp. 794-807, 1997.* [19] Y. Kubota, J. Putkey, and M. Waxham, βNeurogranin Controls the Spatiotemporal Pattern of Postsynaptic Ca2+/CaM signaling,β Biophys. J., vol. 93, pp. 3848-3859, 2007. [20] J.L. McMurry et al., βRate, Affinity and Calcium Dependence of Nitric Oxide Synthase Isoform Binding to the Primary Physiological Regulator Calmodulin,β FEBS J., vol. 278, pp. 4943-4954, 2011. [21] G. Wu, V. Berka, and A. Tsai, βBinding Kinetics of Calmodulin with Target Peptides of Three Nitric Oxide Synthase Isozymes,β J. Inorg. Biochem., vol. 105, pp. 1226-1237, 2011.* [22] M. Zoche et al., βDistinct Molecular Recognition of Calmodulin-Binding Sites in the Neuronal and Macrophage Nitric Oxide Synthases: A Surface Plasmon Resonance Study,β Biochem., vol. 35, pp. 8742-8747, 1996.* [23] E.A. Sheta, K. McMillan, and B.S. Siler Masters, βEvidence for a Bidomain Structure of Constitutive Cerebellar Nitric Oxide Synthase,β J. Biol. Chem., vol. 269, no. 21, pp. 15147-15153, 1994. [24] B.A. Weissman et al., βActivation and Inactivation of Neuronal Nitric Oxide Synthase: Characterization of Ca2+-dependent [125I]Calmodulin Binding,β Eur. J. Pharm., vol. 435, pp. 9-18, 2002. [25] A. Persechini, H.D. White, and K.J. Gansz, βDifferent Mechanisms for Ca2+ Dissociation from Complexes of Calmodulin with Nitric Oxide Synthase or Myosin Light Chain Kinase,β J. Biol. Chem., vol. 271, no. 1, pp. 62-67, 1996. *Not used in calculating average value because study used oligopeptide instead of protein β Used
to establish equivalence of smooth muscle and hippocampal MLCK
β‘Used
to select rates of MLCK association to and dissociation from Ca2+4CaM
Β§Used
to select rates of Ca2+ dissociation from NOS-CaM