Directions. Ready to use sterile poured plates of CAE (Citrate Azide
Enterococcus) agar plate, requires no preparation of media & helps to obtain
exact no. of the ...
CAE (Citrate Azide Enterococcus) Agar Plate
MP1310
For identification of Enterococci in meat, meat products, dairy products and other food stuffs
Directions Ready to use sterile poured plates of CAE (Citrate Azide Enterococcus) agar plate, requires no preparation of media & helps to obtain exact no. of the microorganisms. These plates are very useful in detecting the presence of microorganisms by conventional inoculation method; also growth promotion test can be carried out by ISO 11130. Or Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation Enterococci are widely distributed in the environment, principally inhabiting the human and animal gastrointestinal tract. The resistance of Enterococci to pasteurization temperatures and their adaptability to different substrates and growth conditions (low and high temperature, extreme pH and salinity) implies that they can be found either in food products manufactured from raw materials or in heat-treated food products (4). In general, Enterococci serve as a good index of sanitation and proper holding conditions. CAE (Citrate Azide Enterococcus) Agar Base was initially formulated by Burkwall and Hartmann (1) and later modified by Reuter (2). The medium is used for the identification of enterococci in food products. Casein enzymic hydrolysate and yeast extract serve as sources of carbon, nitrogen, amino acids, vitamins and other essential nutrients. Potassium dihydrogen phosphate has a buffering action. Sodium citrate along with sodium azide helps to inhibit the accompanying contaminating flora. Polysorbate 80 serves as the fatty acid source. Enterococci reduce the colourless 2, 3, 5 Triphenyl Tetrazolium Chloride (FD057) to form a red coloured complex, formazone, thereby imparting a red colour to the enterococcal colonies (3).
Quality Control Appearance Sterile CAE (Citrate Azide Enteroccocus)Agar in 90 mm disposable plates. Colour Yellow coloured medium Quantity of medium 25ml of medium in disposable plate Reaction 6.80- 7.20 Cultural Response Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Sterility test Please refer disclaimer Overleaf.
HiMedia Laboratories
Technical Data
Passes release criteria Cultural Response Organism
