Caffeine intake antagonizes salt sensitive hypertension ... - Nature

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Caffeine+Compound C. 3mM 10mM. αENaC. 170. 140. 95. 72. 55. 43. 34. 21. 17. Marker. (kDa). Cont. 1mM. Caffeine+Compound C. 3mM 10mM. AMPKα. 170.
Caffeine intake antagonizes salt  sensitive hypertension through  improvement of renal sodium handling Supplementary Information Hao Yu#, Tao Yang#, Peng Gao, Xing Wei, Hexuan Zhang, Shiqiang Xiong, Zongshi Lu, Li Li, Xiao Wei, Jing Chen, Yu Zhao, William J. Arendshorst, Qianhui Shang*, Daoyan Liu, Zhiming Zhu* #Authorship

Note: Hao Yu and Tao Yang contributed equally

to this work. *Corresponding author: Zhiming Zhu and Qianhui Shang, E-mail: [email protected] or [email protected] Phone numbers: +86-23-68767849 Fax numbers: +8623-68705094

Supplementary Figure 1. Original Western Blot figures in Figure 4a. Black arrowhead indicates the target band of antibody which is presented in manuscript. αENaC (kDa) Marker 170 140 95 72 55

βENaC

Control

Caffeine (kDa) 170 140 95 72 55 43

43

34

34

21

Marker

Control

Caffeine

17

γENaC (kDa)Marker 170 140 95 72 55 43 34 21

Control Caffeine

34 kDa

Marker

(kDa) 170 140 95 72 55 43 34 21

17

GAPDH

NCC

17

Control

Caffeine

Control

Caffeine

Supplementary Figure 2 Original Western Blot figures in Figures 5a and 5b. Black arrowhead indicates the target band of antibody which is presented in manuscript. .

(a)

αENaC (kDa) Marker 170 140 95 72 55

SGK1

Control

10mM Caffeine

43 34

(kDa) Marker 170 140 95 72 55 43 34

Control

10mM Caffeine

21 17

ERK1/2 (kDa) 170 140 95 72 55 43 34 21

Marker

PKCα

Control

10mM Caffeine (kDa) 170 140 95 72 55 43 34 21

Control

10mM Caffeine

Control

10mM Caffeine

17

17

AMPKα (kDa) Marker 170 140 95 72 55 43 34 21 17

Marker

Control

GAPDH

10mM Caffeine (kDa) 170 140 95 72 55 43 34 21 17

Marker

Supplemental Figure 2 (b)

αENaC Cont Caffeine 1mM 3mM 10mM (kDa) Marker 170 140 95 72 55 43 34

AMPKα

Cont

Caffeine 1mM 3mM 10mM

(kDa) Marker 170 140 95 72 55 43 34 21 17

p-AMPKα Cont (kDa) Marker 170 140 95 72 55 43 34 21

Caffeine

GAPDH

1mM 3mM 10mM

(kDa) Marker 170 140 95 72 55 43 34 21

(kDa) Marker

Caffeine 1mM 3mM 10mM

17

17 αENaC

Cont

Cont Caffeine+Compound C 1mM 3mM 10mM

170 140 95 72 55 43 34

AMPKα (kDa) Marker 170 140 95 72 55 43 34 21

Cont Caffeine+Compound C 1mM 3mM 10mM

17 p-AMPKα (kDa) Marker 170 140 95 72 55 43 34 21 17

Cont Caffeine+Compound C 1mM 3mM 10mM

GAPDH (kDa) Marker 170 140 95 72 55 43 34 21 17

Cont Caffeine+Compound C 1mM 3mM 10mM