Caffeine intake antagonizes salt sensitive hypertension through improvement of renal sodium handling Supplementary Information Hao Yu#, Tao Yang#, Peng Gao, Xing Wei, Hexuan Zhang, Shiqiang Xiong, Zongshi Lu, Li Li, Xiao Wei, Jing Chen, Yu Zhao, William J. Arendshorst, Qianhui Shang*, Daoyan Liu, Zhiming Zhu* #Authorship
Note: Hao Yu and Tao Yang contributed equally
to this work. *Corresponding author: Zhiming Zhu and Qianhui Shang, E-mail:
[email protected] or
[email protected] Phone numbers: +86-23-68767849 Fax numbers: +8623-68705094
Supplementary Figure 1. Original Western Blot figures in Figure 4a. Black arrowhead indicates the target band of antibody which is presented in manuscript. αENaC (kDa) Marker 170 140 95 72 55
βENaC
Control
Caffeine (kDa) 170 140 95 72 55 43
43
34
34
21
Marker
Control
Caffeine
17
γENaC (kDa)Marker 170 140 95 72 55 43 34 21
Control Caffeine
34 kDa
Marker
(kDa) 170 140 95 72 55 43 34 21
17
GAPDH
NCC
17
Control
Caffeine
Control
Caffeine
Supplementary Figure 2 Original Western Blot figures in Figures 5a and 5b. Black arrowhead indicates the target band of antibody which is presented in manuscript. .
(a)
αENaC (kDa) Marker 170 140 95 72 55
SGK1
Control
10mM Caffeine
43 34
(kDa) Marker 170 140 95 72 55 43 34
Control
10mM Caffeine
21 17
ERK1/2 (kDa) 170 140 95 72 55 43 34 21
Marker
PKCα
Control
10mM Caffeine (kDa) 170 140 95 72 55 43 34 21
Control
10mM Caffeine
Control
10mM Caffeine
17
17
AMPKα (kDa) Marker 170 140 95 72 55 43 34 21 17
Marker
Control
GAPDH
10mM Caffeine (kDa) 170 140 95 72 55 43 34 21 17
Marker
Supplemental Figure 2 (b)
αENaC Cont Caffeine 1mM 3mM 10mM (kDa) Marker 170 140 95 72 55 43 34
AMPKα
Cont
Caffeine 1mM 3mM 10mM
(kDa) Marker 170 140 95 72 55 43 34 21 17
p-AMPKα Cont (kDa) Marker 170 140 95 72 55 43 34 21
Caffeine
GAPDH
1mM 3mM 10mM
(kDa) Marker 170 140 95 72 55 43 34 21
(kDa) Marker
Caffeine 1mM 3mM 10mM
17
17 αENaC
Cont
Cont Caffeine+Compound C 1mM 3mM 10mM
170 140 95 72 55 43 34
AMPKα (kDa) Marker 170 140 95 72 55 43 34 21
Cont Caffeine+Compound C 1mM 3mM 10mM
17 p-AMPKα (kDa) Marker 170 140 95 72 55 43 34 21 17
Cont Caffeine+Compound C 1mM 3mM 10mM
GAPDH (kDa) Marker 170 140 95 72 55 43 34 21 17
Cont Caffeine+Compound C 1mM 3mM 10mM
