©2005 FASEB
The FASEB Journal express article 10.1096/fj.05-3673fje. Published online June 28, 2005.
Cathepsin S, a novel biomarker of adiposity: relevance to atherogenesis Soraya Taleb,* Danièle Lacasa,* Jean-Philippe Bastard,† Christine Poitou,* Raffaella Cancello,* Veronique Pelloux,* Nathalie Viguerie,‡ Arriel Benis,*,§ Jean-Daniel Zucker,*,§ Jean-Luc Bouillot,║ Christiane Coussieu,¶ Arnaud Basdevant,* Dominique Langin,‡ and Karine Clement* *INSERM, “Avenir” Team and CHRU Pitié Salpétrière, Hôtel-Dieu Nutrition Department, Centre de Recherche en Nutrition Humaine, F-75004 Paris; †INSERM U680 and CHRU Tenon Hospital, Biochemistry Department, Paris F-75970; ‡INSERM U586, Louis Bugnard Institute, University Paul Sabatier and CHRU Toulouse France, F-31059 Toulouse; §Paris-Nord University, LIM & BIO, Bobigny F-93000; ║Hôtel-Dieu Surgery Department, Paris F-75004; and ¶CHRU Pitié Salpétrière, Hôtel-Dieu Hospital, Hormonology Department, Paris F-75004, France Corresponding author: Dr. Karine Clément, INSERM “Avenir,” Nutrition Department, HôtelDieu, Place du parvis Notre-Dame, 75004 Paris, France. E-mail:
[email protected] ABSTRACT The molecular mechanisms by which obesity increases the risk of cardiovascular diseases are poorly understood. The purpose of this study was to identify candidate biomarkers overexpressed in adipose tissue of obese subjects that could link expanded fat mass to atherosclerosis. We compared gene expression profile in subcutaneous adipose tissue (scWAT) of 28 obese and 11 lean subjects using microarray technology. This analysis identified 240 genes significantly overexpressed in scWAT of obese subjects. The genes were then ranked according to the correlation between gene expression and body mass index (BMI). In this list, the elastolytic cysteine protease cathepsin S was among the highly correlated genes. RT-PCR and Western blotting confirmed the increase in cathepsin S mRNA (P=0.006) and protein (P
