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centrifuge AB FH SBM CD FH FH and SBM separation press ... - MDPI
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centrifuge AB FH SBM CD FH FH and SBM separation press ... - MDPI
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CD34+ HSC/
HSPC
. FSC. SSC. CD56. CD3 granulocytes lymphocytes. CD3+CD56+ NKT cells. CD3-CD56+ NK cells. CD3. CD4. CD45RA. CD25. CD3+CD4+ TÂ ...
A
C
FH and SBM B
FH
separation
press through cell strainer
FH
centrifuge D supernatant cells
SBM
centrifuge
Exclusion of debris and dead cells
PBMC
FSC
CD34+ HSC/HSPC
SSC
SSC
SSC
CD14+ monocytes
CD14
CD34
CD16+ granulocytes
granulocytes
SSC
CD3
CD16
FSC
IgD-CD27+ B cells
CD3-CD56+ NK cells
IgD+CD27+ B cells
CD27
CD3
CD3+CD56+ NKT cells
IgD+CD19+ B cells
IgD
CD56
CD25+CD45RA- T helper cells CD3+CD4+ T helper cells
CD4
CD25
CD25+CD45RA+ T helper cells
CD3
CD45RA
CD25+CD127- Treg cells
CD45RA
CD25
CD3+CD8+ cytotoxicT cells
CD8
CD3
CCR7+CD3+CD4+ T helper cells
CCR7
CD127
CD127
CD127+CD3+CD4+ T helper cells
CD45RA
CD45RA+ cytotoxicT cells
CD25
SSC
lymphocytes
CD19
CD19+CD3- B cells
CD45RA
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