CH2OH OH HO O OH O CH2OH OH OO OH O

CH2OH

CH2OH O Nonreducing ends

O

OH

OH

O O

O

OH

O -1,4 linkage 6 OH CH2OH CH2 CH2OH O

OH

OH

O

O OH

-1,6 linkage

O

OH

O

1

O

CH2OH OH

OH

HO

OH

CH2OH OH

O

O

OH

HO

CH2OH

CH2OH

OH

O O

O OH

4 OH

1

OH

R OH

Figure 4.7.4_fig_001

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_001.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

EC ST

1 5 10 15 20 25 30 35 40 45 50 MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPAVHFGGK RV A 55 60 65 70 75 80 85 90 95 100 FRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVD V L L L G 105 110 115 120 125 130 135 140 145 150 LLPAQQRMKGENWYRGTADAVTQNLKIIRRYKAEYVVILAGDHIYKQDYS 155 160 165 170 175 180 185 190 195 200 RMLIDHVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPP K T 2 D 205 210 215 220 225 230 235 240 245 250 SMPNDPSKSLASMGIYVFDADYLYELLEEDDRDENSSHDFGKDLIPKITE A LG A AA K DA I 255 260 265 270 275 280 285 290 295 300 AGLAYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLASVVPELDMY E M Q H T Q 305 310 315 320 325 330 335 340 345 350 AGLAYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLASVVPELDMY Q HM R E K F I 355 360 365 370 375 380 385 390 395 400 AGLAYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLASVVPELDMY P I D C I 405 410 415 420 425 430 EEDARRFYRSEEGIVLVTREMLRKL-GHKQER Q W

Figure 4.7.4_fig_002

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_002.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

Asn

246

His161

N

O C

NH

NH3 HO

Cys379

SH

O HO

O

HO

OH O

377

Glu

O Lys305

O

NH3+

Arg

O

O– O

NH 300

P

Gly18

HN

H OH

H

OH

Lys15

+

O

Asp21 O–

O

O–

NH3+ O

P

H3N

Tyr355

H H

N

N

HO

NH3 O

N

C N H

Gly354

His356

Figure 4.7.4_fig_003

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_003.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

E. coli Glycogen Synthase 9E 15KTGGLADV 95Y 137DWH 161HN 246NGL

300RL

304QKG 355Y 373PSRFEPCGLTQL

A. tumefaciens Glycogen Synthase 9E 15KTGGLGDV 96Y 138DWG 162HN 246NGL 300RL 304QKG 355Y 373PSRFEPCGLTQL Streptococcus pneumoniae Glycogen Synthase 9E 15KTGGLGDV 95Y 133DWG 163HN 247NGL 301RL 306QKG 356Y 374PSRFEPCGLSQL Pyrococcus abyssi Glycogen Synthase 9E 14KVGGLGDV 89Y 128DWG 151HN 217NGF 257RF 262QKG 317L 335PSYFEPFGLTQL Anabaena sp. PCC 7120 9E 15KVGGMGDV 96Y 129DWH 152HN 262NGI 280RL 284QKG 336Y 334PSRFEPCGISQM C. reinhardtii granule-bound starch synthase 156E 162KTGGLGDV 259Y 294DWH 323HN 416NGL 470RL 474QKG 526Y 544PSMFEPCGLTQL Barley granule-bound starch synthase 84E 90KTGGLGDV 176Y 227DWH 257HN 346NGL 401RL 405QKG 457F 475TSRFEPCGLIQL Maize granule-bound starch synthase 85E 91KTGGMLGDV 177Y 228DWH 258HN 347NGL 402RL 406QKG 459F 477TSRFEPCGLIQL Potato granule-bound starch synthase 89E 95KTGGLGDV 181Y 232DWH 262HN 351NGL 406RL 410QKG 461F 480PSRFEPCGLIQL Rice granule-bound starch synthase 91E 97KTGGLGDV 183Y 234DWH 264HN 353NGL 408RL 412QKG 463F 481PSRFEPCGLIQL Maize soluble starch synthase I 140E 146KTGGLGDV 232Y 277DWH 307HN 401NGL 455RL 459QKG 510F 258PSRFEPCGLNQL Potato soluble starch synthase I 139E 145KTGGLGDV 232Y 277DWH 307HN 401NGL 455RL 459QKG 510F 258PSRFEPCGLNQL Wheat soluble starch synthase I 147E 153KTGGLGDV 239Y 284DWH 314HN 408NGL 462RL 466QKG 517F 235PSRFEPCGLNQL Wheat soluble starch synthase IIa 316E 322KTGGLGDV 403Y 446DWH 476HN 562NGL 621RL 625QKG 676F 694PSRFEPCGLNQL Maize soluble starch synthase IIa 249E 255KTGGLGDV 333Y 376DWH 406HN 492NGL 551RL 555QKG 606F 624PSRFEPCGLNQL Potato soluble starch synthase II 284E 290KTGGLGDV 371Y 414DWH 444HN 530NGL 589RL 593KG 644F 662PSRFEPCGLNQL Potato soluble starch synthase III 788E 794KTGGLGDV 875Y 909DWS 938HN 986NGL 1040RL 1044QKG1100Y 1118PSIFEPCGLTQL Wheat SSIII 1188E 1194KTGGLGDV1275Y1308DWS1337HN 1385NGL 1439RL 1443QKG1499Y 1517PSIFEPCGLTQL

Figure 4.7.4_fig_004

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_004.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

11 10 9 8 7 6 5 4 3

Population, %

2 1 0

6

8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40

6

8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40

11 10 9 8 7 6 5 4 3 2 1 0

D.P.

Figure 4.7.4_fig_005

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_005.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

asd

0

glgB

2

glgX

glgC

4

6

glgA

8

glgP

10

glgD

12

14

Kilobases

Figure 4.7.4_fig_006

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_006.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

E. coli consensus CRP binding site E. coli transcript B S. typhimurium transcript

NAANTGTGANNTNNN-TCACATTT ** * ** * ** * *** AAATTCTGCTCTCGGATCGCTTTT ** * ** * ** * *** AATTCTGCTCTCGGATCGCTTT

Figure 4.7.4_fig_007

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_007.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

Consensus

–35 TTGACA

glgC transcripts

–10 TATAAT

A

**** TTCGCTGGAGAGGATAACCCAGTGATTACGGCTGTCTGGCA

B

******** TTGATCGCAATTAACGCCACGCTTGAGGTAACAGAGATTCTTT

C

*** AGGCGACGGCAATGTCCGTTGCTAAATCGATATGCT

Figure 4.7.4_fig_008

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_008.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date:

D

C

B

A

glg X

glg C CRP Binding Site

mutations that enhance glg C transcription glg R (cis)

+

glg Q (trans)

+

+

+

+

glg A (trans)

+

+

+

+

Figure 4.7.4_fig_009

EcoSal

Final size (w x h)

File name ecosal_4.7.4_fig_009.eps

Date (M/D/Y) 09/10/14

Author's review ___ okay ___ corrections Initials/date: