Changes in the antioxidative enzyme activities and lipid ... - SciELO

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5- &'; GV CN

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21: '%  DQVJ KP TQQVU CPF UJQQVU WPFGT KFGPVKECNGZRGTKOGPVCNEQPFKVKQPU

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1:++8' 564'55 +0 %#&/+7/ #0& .'#&64'#6'& 9*'#6 5''&.+0)5

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

YKVJQWVGP\[OGCPF8KUVJGCDUQTDCPEGQHVJGUCORNG

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

4'57.65 4GNCVKXG VQ EQPVTQN RNCPVU ITQYVJ QH VJG YJGCV UGGFNKPIUYCUFTCUVKECNN[TGFWEGFFWGVQ%FCPF2DCVCNN VGUVGF EQPEGPVTCVKQPU (KIWTG  (QT %F VJGTG YCU CP CNOQUV EQORNGVG KPJKDKVKQP QH ITQYVJ HTQO  z/ QPYCTFUGXGPCVz/%FITQYVJYCUKPJKDKVGF ` KP DQVJ TQQVU CPF UJQQVU 6JGTGHQTG CNN VJG CPCN[VKECN UVWFKGUYGTGTGUVTKEVGFVQVTGCVOGPVUKPVJGTCPIGQH

$TC\,2NCPV2J[UKQN 



5- &'; GV CN

z/ QH %F (QT 2D ITQYVJ QH TQQVU CPF UJQQVU YCU UKIPKHKECPVN[ TGFWEGF GXGP CV VJG NQYGUV EQPEGPVTCVKQP

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15

A

a

Root

a

Shoot

Length, cm

12 9

b

6 b

c d

3

0

0

50

15

e f

f

c

d

d

d

100

200

500

1000 2000

d

B

a a

Length, cm

12

9 b

6

c d

3

b

e

c d

0

0

200 500 1000 Concentration (µM)

e 2000

(KIWTG%JCPIGUKPVJGTQQVCPFUJQQVNGPIVJQHFQNF YJGCV UGGFNKPIU ITQYP KP RTGUGPEG QH KPETGUKPI EQPEGPVTCVKQPUQH%F%N  #CPF2D 01 $UGRCTCVGN[ 6JG XCNWGU CTG VJG OGCP v 5& QH VJTGG KPFGRGPFGPV GZRGTKOGPVUGCEJYKVJVJTGGTGRNKECVGU6JGOGCPXCNWGU QHCRCTVKEWNCTVKUUWGV[RG GKVJGTTQQVQTUJQQVHQNNQYGF D[VJGUCOGNGVVGTCTGPQVUKIPKHKECPVN[FKHHGTGPV 2 .5&VGUV

$TC\,2NCPV2J[UKQN 

KPUJQQVVKUUWGUTGCEJKPIUKIPKHKECPEGGXGPCVVJGNQYGUV %FEQPEGPVTCVKQPVGUVGF z/YJGTGCUKPTQQVVKUUWGU VJGTGYCUCEQORNGVGNQUUQH51&CEVKXKV[UVCTVKPIHTQO z/QPYCTFU6JGCEVKXKV[QH%#6PGCTN[JCNXGFKPTQQVU CVz/QH%FCPFVJGTGCHVGTUOCNNEJCPIGUYGTGPQVGF CV JKIJGT %F EQPEGPVTCVKQPU (KIWTG  $[ EQPVTCUV KP UJQQV VKUUWGU VJG FGENKPG KP %#6 CEVKXKV[ YCU PQV UQ UJCTRGIKVYCUCVz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z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

(KIWTG  *QYGXGT %FKPFWEGF KPETGCUGF /&# CEEWOWNCVKQPTGCEJGFUVCVKUVKECNUKIPKHKECPEGQPN[CV z/QH%F(QT2D/&#YCUUKIPKHKECPVN[NCTIGTKPTQQV VKUUWGU CV  CPF  z/ VJCP KP EQPVTQN RNCPVU YJGTGCU KP UJQQVU UWEJ FKHHGTGPEG UVCTVGF QEEWTTKPI CV  z/ 2D DWV YKVJ PQ CRRTGEKCDNG EJCPIG CV JKIJGT EQPEGPVTCVKQPU

&+5%755+10 +PVJGRTGUGPVUVWF[VJGOQUVRTQOKPGPVU[ORVQOQH %F CUYGNNCU2DVQZKEKV[YCUHQWPFVQDGVJGUVWPVGF ITQYVJ QH FQNF UGGFNKPIU .KMG QVJGT UVTGUUGU %F UVTGUU KU CNUQ GZRGEVGF VQ CNVGT VJG CEVKXKVKGU QH



1:++8' 564'55 +0 %#&/+7/ #0& .'#&64'#6'& 9*'#6 5''&.+0)5

6

60

A

a

SOD, U mg-1 Protein

2

d d

c

1.6

1.8

B

1.5

1.2

b c a

d

b

c d

0.4

a

c

0

0.8

b

24

0 a

CAT, nkatal mg-1 Protein

36

12

b

CAT, nkatal mg-1 Protein

SOD, U mg-1 Protein

4

a

Shoot

48

Shoot

b

A

Root

Root

b

c

cd cd

B a

1.2 b

c

0.9 a

0.6

b

d c

e d

e

0.3 0

0

b

0.28 POX, mkatal mg-1 Protein

1

C

a

POX, mkatal mg-1 Protein

0.35

c 0.21

d

0.14 0.07 d 0

0

c

b

50 100 CdCl2, µM

c 0.6 0.4

d

e 0.2 e 0

(KIWTG%JCPIGUKPVJGURGEKHKECEVKXKVKGUQHUWRGTQZKFG FKUOWVCUG 51& # ECVCNCUG %#6 $ CPF IWCKCEQN RGTQZKFCUG 21: %KPTQQVCPFUJQQVVKUUWGUQHFQNF YJGCV UGGFNKPIU ITQYP KP RTGUGPEG QH KPETGUKPI EQPEGPVTCVKQPUQH%F%N 5GGNGIGPFVQ(KIWTGHQTFGVCKNU

b

0.8

a 200

a

C

0

d

c

b

200 500 1000 Pb(NO3)2, µM

a 2000

(KIWTG%JCPIGUKPVJGURGEKHKECEVKXKVKGUQHUWRGTQZKFG FKUOWVCUG 51& # ECVCNCUG %#6 $ CPF IWCKCEQN RGTQZKFCUG 21: %KPTQQVCPFUJQQVVKUUWGUQHFQNF YJGCV UGGFNKPIU ITQYP KP RTGUGPEG QH KPETGCUKPI EQPEGPVTCVKQPU QH 2D 01 5GG NGIGPF VQ (KIWTG  HQT FGVCKNU $TC\,2NCPV2J[UKQN 



5- &'; GV CN

80

A

Root

MDA, nmol g-1 FW

Shoot

a

60

ab

ab

b

20

b

0

50

100

100

200

B

80

MDA, nmol g-1 FW

ab

ab 40

a

a

a

a

a

a ab

60 bc c 40

20

c b

0

200 500 1000 Concentration (µM)

2000

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

$TC\,2NCPV2J[UKQN 

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