and purification of the associated anaerobic sulfite reductase. ... reductase was purified from the cytoplasmic fraction of the anaerobically grown cysI mutant and ...
Characterization of Anaerobic Sulfite Reduction by Salmonella typhimurium and Purification of the Anaerobically Induced Sulfite Reductase PATRICK C. HALLENBECK,' MARTA A. CLARK,2 AND ERICKA L. BARRETT3* Departement de Microbiologie et Immunologie, Universite de Montreal, Montreal, Quebec, Canada H3C 3J7,' and Department of Bacteriology2 and Department of Food Science,3 University of California, Davis, California 95616 Received 30 September 1988/Accepted 5 March 1989
Mutants of Salmonella typhimurium that lack the biosynthetic sulfite reductase (cysI and cysJ mutants) retain the ability to reduce sulfite for growth under anaerobic conditions (E. L. Barrett and G. W. Chang, J. Gen. Microbiol., 115:513-516, 1979). Here we report studies of sulfite reduction by a cysI mutant of S. typhimurium and purification of the associated anaerobic sulfite reductase. Sulfite reduction for anaerobic growth did not require a reducing atmosphere but was prevented by an argon atmosphere contaminated with air (
