Characterization of Bacteroides forsythus Isolates - Journal of Clinical ...
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Characterization of Bacteroides forsythus Isolates - Journal of Clinical ...
(Johns-Manville, Denver, Colo.). The carrier gas was nitrogen (30 ml/min), the injection port temperature was 150°C, and the oven temperature was 120°C.
Characterization of Bacteroides forsythus Isolates T. TAKEMOTO,1 H. KURIHARA,1
AND
G. DAHLEN2*
Department of Endodontology and Periodontology, Hiroshima University School of Dentistry, Hiroshima, Japan,1 and Department of Oral Microbiology, Go ¨teborg University, Go ¨teborg, Sweden2 Received 3 September 1996/Returned for modification 18 October 1996/Accepted 7 March 1997
Fifteen Bacteroides forsythus strains freshly isolated from patients with periodontitis were used together with three collection strains and one type strain for characterization of growth on various media; determination of enzymatic profiles, antibiotic susceptibility profiles, 16S rRNA ribotypes, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) outer membrane protein profiles, and pathogenicity; and gas chromatography analysis by using a wound chamber model in rabbits. All strains were stimulated by N-acetylmuramic acid, while one strain needed a further supplement such as yeast extract for optimal growth. All strains showed trypsin-like activity. While 10 different ribotypes were found, the SDS-PAGE profiles revealed similar patterns for all strains. All strains were sensitive to penicillin G (MICs,
