ported the purification of iota-toxin from cultures of C. per- fringens type E. ..... purified variant components showed only one band of approx- imately 43 kDa on ...
Characterization of the Enzymatic Component of Clostridium perfringens Iota-Toxin MASAHIRO NAGAHAMA, YOSHIHIKO SAKAGUCHI, KEIKO KOBAYASHI, SADAYUKI OCHI, AND JUN SAKURAI* Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan Received 4 October 1999/Accepted 20 January 2000
The iotaa component (ia) of Clostridium perfringens ADP ribosylates nonmuscle /␥ actin and skeletal muscle ␣-actin. Replacement of Arg-295 in ia with alanine led to a complete loss of NADⴙ-glycohydrolase (NADase) and ADP-ribosyltransferase (ARTase); that of the residue with lysine caused a drastic reduction in NADase and ARTase activities (