IJSEM Papers in Press. Published August 1, 2013 as doi:10.1099/ijs.0.049726-0
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Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of
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chick pea (Cicer arietinum L.)
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Kathiravan, R., 1Jegan, S., 1Ganga, V., 1*Prabavathy, V.R., 3Tushar, L., 2Sasikala, Ch. and
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Ramana, Ch.V.
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Microbiology Department, M.S. Swaminathan Research Foundation, 3rd Cross street, Taramani
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institutional area, Chennai 600113, India
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University Hyderabad, Kukatpally, Hyderabad 500085, India
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University, Gachibowli, Hyderabad, 500046, India
Bacterial Discovery laboratory, Centre for Environment, Institute of Science and Technology, JNT
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central
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Corresponding author: Dr. V. R. Prabavathy E-mail:
[email protected],
[email protected]
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The GenBank accession numbers for 16S rRNA, recA, dnaK, thrC and nifH genes of strain
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MSSRFBL1T are JQ230000, KC189949, KC189950, KC189951 and KC189952, respectively.
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A comparison of the total rhizobiaceae family, 16S rRNA, recA, dnaK, thrC, polar lipids and
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hopanoids are available as supplementary material with the online version of this paper.
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Taxonomic position of a strain MSSRFBL1T isolated from chickpea rhizosphere soil from
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Kannivadi, India was determined. Strain MSSRFBL1T forms bluish black colonies, stains Gram-
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negative, motile, aerobic, capable of fixing dinitrogen, oxidase negative and catalase positive. Q-10
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was the major respiratory quinone. Major fatty acids of strain MSSRFBL1T were C18:1ω7c and
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C19:0cycloω8c. Minor amounts of C18:0, C12:0, C14:03OH, C18:03OH, C16:0, C16:1ω6c/C16:1ω7c,
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C17:0iso3OH, C18:0 and C20:1ω7c were also present. Polar lipids include diphosphatidylglycerol,
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phosphatidylethanolamine;
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unidentified
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bishomohopanediol, adenosylhopane, and 2β- methyl bacteriohopanetetrol are the major hopanoids
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of strain MSSRFBL1T. The genomic G+C content was 71 mol %. EzTaxon-e based BLAST
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analysis of 16S rRNA gene indicated its highest similarity with Ensifer adhaerens LMG20216T
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(97.3 %) and other members of the genus Ensifer (
