Clinical Applications of Cryobiology

Clinical Applications of Cryobiology Editors

Barry J. Fuller, Ph.D. Senior Lecturer University Department of Surgery Royal Free Hospital School of Medicine University of London London, U.K.

Brian W. W. Grout, Ph.D. Head of Research and Development Novalai, pie Great Dunmow, U.K.

CRC Press Boca Raton

Ann Arbor

Boston

London

Library of Congress Cataloging-in-Publication

Data

Clinieal applieations of eryobiology I editors, Barry J. Fuller, Brian W. W. Grout. p. em. Includes bibliographical referenees and index. ISBN 0-8493-5429-3 I. Cryobiology. 2. Cryopreservation of organs, tissues, etc. l. Fuller, B. J. II. Grout, B. W. W. [DNLM: I. Cryopreservation. 2. Cryosurgery. 3. Hypothermia, Indueed. 4. Tissue Preservation. QH 324.9 C64lj QH324.9.C7C58 1991 574.19'167--de20 DNLMlDLC for Library of Congress

91-10865 CIP

This book represents information obtained from authentie and highly regarded sourees. Reprinted material is quoted with permission, and sourees are indieated. A wide variety of referenees are listed. Every reasonable effort has been made to give reliable data and information, but the author and the publisher eannot assume responsibility for the validity of a11materials or for the eonsequenees of their use. All rights reserved. This book, or any parts thereof, may not be reprodueed in any form without written eonsent from the publisher. Direet a11inquiries to CRC Press, Ine., 2000 Corporate

Blvd., N.W.,

Boca Raton, Florida 33431.

e 1991 by CRC Press, lne.

International

Standard Book Number 0-8493-5429-3

Library of Congress Card Number 91-10865 Printed in the United States of Ameriea I 2 3 4 5 6 7 8 9 0

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766

Clinical Applications oj Cryobiology

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102

ClinicaL Applications

of Cryobiology

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B. bCJKGIC=yD etefctrgxit cF 7EBC2[abecc tg p_4EB stfrexqts ube gwt uxefg gx`t gwt fhrrtffuh_ relbcetfteipgxba bu telgwebrlgtf hfxav wlsebkltgwl_ fgperw 0TQg1 pas _xdhxs axgebvta2 rb`cpexav gwt tuuxrprl bu gwt relbcebgtrgpag gb gwpg bu cb_lixal_ cleeb_xsbat 0eje14 Tbjtite2 gwtl cetuteets TQg gb eje pf gwt _pggte xf etgpxats gb p rbafxstepq_t tkgtag xa gwt etrxcxtag4 hjb ltpef _pgte2 [abecc tg p_4EC etrtxits gwt phgwbexmpgxba ube uxefg in vivo gexp_f xa wh`paf4 hwtl tkp`xats gwt qtwpixbe bu q_bbs vebhc rb`cpgxq_t telgwebrlgtf relbcetfteits jxgw TQg ba p gbgp_ bu 7Acpgxtagf2 uxasxav p cpep__t_ qtgjtta in vitro fp_xat fgpqx_xgl pas in vivo fheixip_ epgt4 hwt dhpagxgl bu gepafuhfts ets q_bbs rbechfr_tf fgx__ rxerh_pgxav 8: w pugte gepafuhfxba jpf pf wxvwpf DA- xa ftita rpftf4 fbqfba., jwb jpf pq_t gb rbauxe` gwt etfh_gf bu [abecc tg p_4EB cbxagts bhg gwpg tprw `paxch_pgxba atrtffpel pugte gwpjxav jbh_s etshrt gwt tfftagxp_ psipagpvt bu qxbrb`cpgxq_t tkgeprt__h_pe relbcebgtrgpagf2 ap`t_l gwt uprg gwpg gwt q_bbs `pl qt gepafuhfts jxgwbhg et`bip_ bu gwt relbcebgtrgpag ql jpfwxav4 hjb vep`f be `bet bu uett wt`bv_bqxa jwxrw

103 `pl qt et_tpfts xa p jwb_t haxg bu q_bbs ql gwt relbcetfteipgxba cebrtff jbh_s cebwxqxg ebhgxat hft ube rxix_ checbftf4 L jpfwxav cebrtshet tkp`xats ql [abecc tg p_4KKfwbjts gwpg gwt fp_xat fgpqx_xgl pugte gwpjxav rbeetfcbasts jxgw gwt etrbitel ubhas pugte jpfwxav4 La xageprt__h_pe _bff bu cb3 gpffxh` bu 7A- brrheets shexav gwt uettmt3gwpj cebrtff pas shexav gwt et`bip_ bu gwt relbcebgtrgpag4 Tbjtite2 shexav gwt et`bip_ bu TQg2 uett wt`bv_bqxa jpf p_fb etshrts gb D49- bu gwt bexvxap_ dhpagxgl ubhas pugte gwpjxav pas p`bhagts gb pa pitepvt bu 9: `v5 766 `_4 hwt phgwbef uhegwte tkp`xats cebrtshetf gb et`bit gwt relbpssxgxit atrtffpel ube r_xaxrp_ ebhgxat ql stgte`xaxav gwt fheixip_ epgt bu epsxb3Upqt_ts telgwebrlgtf pugte xauhfxba xa cex`pgtf4 766 Uapssxgxba2 gwt qxbrwt`xrp__l xagtetfgxav Lhe pas 8293PeS jtet tkp`xats xa pax`p_ tkctex`tagfG p stretpft bu 8A- pas 86-2 etfctrgxit_l2 jpf ubhas pugte jpfwxav2 gbvtgwte jxgw p :A- _bff bu xageprt__h_pe cbgpffxh` pas ab rwpavt xa gwt prgxixgl bu gwt taml`t v_hrbft3B3cwbfcwpgt3stwlsebvtapft 0SBe3PT14 ktpgwteqtt tg p_4767 xaretpfts gwt fp`cxt ib_h`t gb :66 `_ ql hfxav c_pfgxr qpvf2 etrbvaxmxav gwt x`cbegpart bu `pxagpxaxav p rbafgpag gwxr^atff p__ bite gwt fp`cxt shexav uettmxav pas gwpjxav4 Nbhfxatph tg p_4768 rbar_hsts ueb` tkctex`tagf jxgwsbvf gwpg gwtet `xvwgqt 8 sxuutetag cbch_pgxbaf bu ets q_bbs rbechfr_tf pugte relbcetfteipgxba jxgw etvpes gb gwt in vivo wp_u3 _xut4 Rbetfg tg p_4769 tkp`xats sxuutetag t_trgeb_lgt `tsxp xa rb`qxapgxba jxgwTQg2 qhg ubhas ab fxvaxuxrpagsxuutetart ube A sxuutetag rpgtvbextf 0xar_hsxav xfbgbaxr fp_xat2 [etqf3fxavte fb_hgxba1 jxgw etvpes gb uett wt`bv_bqxa2 cbgpffxh`2 fbsxh` xa gwt fhct`pgpag2 bf`bgxr etfxfgpart2 pas rbechfr_t fxmt4 La tkrwpavt bu xageprt__h_pe cbgpffxh` pas fbsxh` brrheexav shexav gwt uettmxav3gwpjxav cebrtff jxgwbhg fhqftdhtag wt`b_lfxf jpf stgtrgts ql btel`pa pas Tbeaq_bjte.K. jwta tkp`xaxav ipexbhf `preb`b_trh_pe relbcebgtrgpagf4 Lg _tpfg p cpeg bu gwt tkrwpavt bu gwt rpgxbaf gbb^ c_prt shexav gwpjxav2 jwxrw `xvwg wpit qtta sht gb p gt`cbepel _bff bu gwt ft`xcte`tpqx_xgl bu gwt rt__ `t`qepat4 Mppe.K,ubhas pa xaretpft bu gwt relbcebgtrgxit tuutrg bu TQg ql p_qh`xa4 L_gwbhvw gwt pssxgxit xf abg cetftag jxgwxagwt rt__f2 TQg rbartagepgxbaf fgpgts pet rb``ba_l et_pgts gb gwt jwb_t fp`cxt jtxvwg be ib_h`t 0xar_hsxav gwt rt__f14 ifxav p fp`cxt ib_h`t bu 7 `_2 pa xaretpft bu gwt fp_xat fgpqx_xgl ueb` DC- gb E9- rbh_s qt bqfteits xa gwt cetftart bu 7:- p_qh`xa xa p 78 jg- TQg fb_hgxba4 hwxf xf abg fhecexfxav pf p_qh`xa wpf fx`x_pe rb__bxsp_ cebctegxtf pf TQg4 Nb`cpets gb gwt hagetpgts rbageb_ p_fb Lhe pas 8293PeS stretpfts ba_l ql B- pas 8-2 etfctrgxit_l4 Rb__bjxav etcbegf bu rbpvh_bcpgwxtf rphfts ql TQg2 ktpgwteqtt tg p_476B tkpeaxats gwxf cwtab`taba xa tkctex`tagf ba cex`pgtf4 hwtl rbh_s abg stgtrg pal ceb_bavpgxba bu gwt q_ttsxav gx`t be pa xaretpfts gtastarl gbjpesf q_ttsxav pugte ps`xaxfgepgxba bu TQg3rel3 bcetfteits wb`b_bvbhf be phgb_bvbhf q_bbs4 Txfgbcpgwb_bvxrp_ tkpeaxapgxba bu qxbcfl pas phgbcfl `pgtexp_ sxs abg fwbj pal p_gtepgxbaf jwxrw `xvwg qt sht gb gwt relbcebgtrgpag4 hwxf ftt`f fhecexfxav fxart hc gb 96 gb A6- bu gwt pax`p_f. q_bbs ib_h`t wps qtta etc_prts ql relbcetfteits hajpfwts telgwebrlgtf xa 7: jg- TQg 0gwt `b_trh_pe jtxvwg bugwt TQg2 wbjtite2 qtxav upxe_l _bj2 x4t428D2666 gb :A2666 v5`b_14 ktpgwteqtt tg p_476C p_fb gtfgts gwt xau_htart bu c_pf`p ba gwt relbcetfteipgxba cebrtff jxgw TQg4 hwtl ubhas gwpg gwt fp_xat fgpqx_xgl pugte gwpjxav jpf xaretpfts ql D- gb vxit D9- xu2cexbe gb uettmxav2 gwt c_pf`p wps qtta et`bits ql jpfwxav bart jxgwxfbgbaxr fp_xat4 Tbjtite2 gwxfpcc_xts ba_l ube p 7:- TQg fb_hgxbaG jwta 76- TQg jpf hfts2 jpf wxav jxgw sxuutetag t_trgeb_lgt fb_hgxbaf sxs abg _tps gb fhctexbe etfh_gf pugte gwpjxav476D ktpgwteqtt tg p_4 gwtetubet t`3 cwpfxmts pvpxa gwt x`cbegpart bu gwt pccebcexpgt fgperw rbartagepgxba2 x4t42 7:-4 L_etpsl xa 7ECA2axbatggx pas Thag.K, fwbjts itel r_tpe_l gwt cebq_t`f et_pgts gb gwt stgte`xapgxba bu gwt etrbitel epgt xa cetftart bu TQg4 L etrbitel epgt bu EC- bu gwt rt__f pugte relbcetfteipgxba rbeetfcbasts gb p fp_xat fgpqx_xgl bu ba_l CB-4 hwxegl `xahgtf pugte

104

Clinical Applications of Cryobiology

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106

0/ Cryobiology

Clinical Applications

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108

Clinical Applications oj Cryobiology

A6 3

B66 Trgn-o

RUSifQ 84 Ptctastatt bu gwt fheixip_ epgt 0g12 `tpfhets ql `tpaf bu fp_xat fgpqx_xgl2 ba wt`pgbrexg 0Ttg1 pas TQg tbattagepgxba xa gwt tkgeprt__h_pe fcptt4 Nbb_xav epgt 8:6 [meaxa2 fp`cxt ib_h`t 7: `_2 .fp`c_t gwxt^atff 9 ``2 jpe`xav epgt :66 [W`xa2 fgpetw `bsxuxtpgxba TQg 40/0.55. hwt qb_s _xatf tbaatrg cbxagf bu p wt`pgbrexg bu :6pas p tkgeptt__h_pe fgperw rbattagepgxba bu f_xvwg_l _tff gwpa 8A jg-4 hwt wpgtwts c_pat etcetftagf p epavt bu xagtetfg ube ceptgxtp_ pcc_xtpgxba4 0Ppgp ueb` Stxfte2 T., Diss. Med. Fak., fkhT Lptwta2 xa cetcpepgxba41

hwtft xaitfgxvpgxbaf ba gwt xagteprgxbaf bu wt`pgbrexg2 TQg rbartagepgxba2 pas bcgx`h` rbb_xav epgt tarbhepvts [seqte tg p_479Dgb fhvvtfg p r7xaxrp__l pcc_xrpq_t bat3fgtc relbcetf3 teipgxba cebrtshet4 hwt bcgx`xmpgxba bu cetcpepgxit stgpx_f.F, 0t4v42 rb`cbfxgxba bu gwt relbcebgtrgxit fb_hgxba2 et`bip_ bu c_pf`p2 c_pgt_tgf2 pas _th^brlgtf qtubet uettmxav1 pf jtx_ pf gwt t`c_bl`tag bu fhxgpq_t uettmxav rbagpxatef2 tapq_ts hf gb prwxtit p fheixip_ bu E9 ± 9- 0tfgx`pgts ql fp_xat fgpqx_xgl1bu gwt xaxgxp__luebmta fMNf4 79ERhegwte xaitfgxvpgxbaf ba gwt `tgpqb_xgtf Lhe pas 8293PeS etitp_ts_9B47:b ba_l f_xvwg stretpftf pugte relbcetf3 teipgxba xa cetftart bu TQg4 Rhghet etftperw ba in vivo wp_u3Uxut bu gwt gwpjts ets q_bbs rt__f pas `pkx`h` p`bhag bu gb_tepq_t uett wt`bv_bqxa eaxvwg fwbj gwt prrtcgpqx_xgl bu gwxfbat3fgtc cebrtshet4 Lf qbgw TQg pas wt`bv_bqxa ueb` stfgeblts rt__f pet cetftag ba_l xa gwt tkgeprt__h_pe fcprt2 gwtl rpa tpfx_l qt et`bits ql sxfrpesxav gwt fhct`pgpag pugte ftsx`tagpgxba bu gwt ets q_bbs rt__f4

Q4 cgrPnocaDochgn

hW WpgJDfPgoDe cgoPlPno cg GluhFchehau Uafgtps bu etcbegxav gwt tabeeabhf ah`qte bu in vitro xaitfgxvpgxbaf ba ets q_bbs rt__f pg _bj gt`ctepghetf xa gwt cpfg strpst2 gwt phgwbef jx__ gel gb fh``pexmt fb`t ft_trgts fghsxtf jwxrw ueb` gwtxe cbxag bu ixtj st`bafgepgt gwt ipexbhf pfctrgf bu gwt cebq_t`4 L utj ^tl jbesf2 fhrw pf tuutrgf ba `t`qepatf2 bf`b`tgexr qtwpixbe2 xageprt__h_pe uettmxav2

xau_htart bu rt__ rbartagepgxba2 glct pas rbartagepgxba

bu gwt relbcebgtrgpag2

rbb_xav pas

76E jpe`xav epgtf2 pagxbkxspagf2 stube`pqx_xgl2 xba qp_part2 pas taml`tf uxas p cpgw gwebhvw gwt ah`tebhf pfctrgf jxgwbhg r7px`xav rb`c_tgtatff ^tljbesf pas gwt rbagexqhgxbaf etcbegts4

`pl qt wt_cuh_ gb jxgwetvpes gb gwt

74 bt`qepatf Uagprg be etfgbepq_t `t`qepat xagtvexgl xf sbhqg_tff pcetetdhxfxgt ube p uh__l ixpq_t rt__4 Pht gb gwt fb_hgt taexrw`tag shexav uettmxav MpU_pf7:7 pas Mp__pf tg p_47:8 bqfteits rwpavtf xa gwt v_lrtep_stwlst393cwbfcwpgt stwlsebvtapft prgxixgl xa ets rt__f4 hwxf v_lrb_lgxr ta3 ml`t xfpa tkgexafxr cebgtxa _brp_xmts ba gwt xaate fheuprt bu gwt `t`qepat pas xgf pffbrxpgxba jxgw pas sxffbrxpgxba ueb` gwt `t`qepat xf etvh_pgts ql gwt gbaxrxgl bu gwt fheebhasxav `tsxh`4 Uacetftart bu v_lrteb_ shexav p uettmt3gwpj rlr_t gwt et_tpft bu gwt taml`t jpf `pe^ts_l stretpfts2 xasxrpgxav p _tff sxfgheqts `t`qepat pf p rbaftdhtart bu gwt rel3 bcebgtrgpag4 Rettmt3ueprghet pas 3tgrwxav fghsxtf ba wh`pa telgwebrlgtf ql Rhyx^pjp,K etitp_ts h_gepfgehrghep_ p_gtepgxbaf bu gwt `t`qepatf pf p etfh_g bu xageprt__h_pe xrt ube`pgxba4 _g jpf fhvvtfgts gwpg2pf p rbaftdhtart gb gwt sxfbeste xashrts ql gwt sxetrg rbagprg qtgjtta xrt relfgp_f 0648 gb 846 U3a`xa sxp`tgte1 pas `t`qepatf2 wt`b_lfxf shexav gwpjxav jbh_s brrhe4 Rettmt3ueprghexav jxgwbhgtgrwxav upx_ts gb vxit pal xaube`pgxbaba gwtft h_gepfgehrghep_ p_gtepgxbaf4 Qkcbfhet bu ets q_bbs rt__f gb wxvwfp_g rbartagepgxbaf pg fhqmteb gt`ctepghetf _ts gb p gt`ctepghet3stctastag ftvetvpgxba bu `t`qepat rbafgxghtagf4 hwt `xrebitfxr_tf cebshrts pg sxuutetag gt`ctepghetf ipexts xagwtxe rb`cbfxgxba2 gwhf xasxrpgxav gwpg wt`b_lfxf xf pa titag stctasxav ba qbgw bf`bgxr cetffhet pas gt`ctepghet4 Ua beste gb sxfgxavhxfw qtgjtta gwt sxetrg tuutrg bu wxvwfp_g rbartagepgxbaf pas gwt fwexa^pvt qtlbas p rexgxrp_ _x`xg xa cebshrxav `t`qepat t_tipgxbaf be cpgrwtf2 Sbt^bbc pas gcxtf.F, ipexts ib_h`t pas fp_g rbartagepgxba xastctastag_l4 Rettmt3tgrw t_trgeba `xrebfrbcl etitp_ts gwpggwt rwpavtf `bet _x^t_l stctasts ba gwt etshrgxba xa ib_h`t gwpa jtet p sxetrg tuutrg bu gwt fp_g ba gwt `t`qepat4 hwt rb`qxapgxba bu etfh_gf bqgpxats ql t_trgeba cpep`pvatgxr etfbapart 0Qef12 fcxa cebqt gtrwaxdht pas uettmt3ueprghet t_trgeba eaxrebfrbcl _ts hp^pwpfwx tg p_47:A gb gwt fhvvtfgxba bu p gepafxgxba xa gwt `t`qepat jwta gt`ctepghet xf _bjtets qt_bj UdoN4da rbb_xav bf`bgxrp__l fgetffts 0748 df`b__^v1 ets q_bbs rt__f qt_bj gwxfgt`ctepghet p `pe^ts sxfrbagxahxgl bu gwt ebgpgxbap_ rbeet_pgxba gx`t gbvtgwte jxgwgwt stit_bc`tag bu _pevt jexa^_tf pas pvvetvpgxba bu `t`qepat cpegxr_tf brrheets4 Nwpavtf xa gwt cbfxgxbaf bu cebgtxaf jxgwxa gwt _xcxs cwpft bu telgwebrlgt vwbfg `t`qepatf jtet p_fb xashrts ql gwt pssxgxba bu rel3 bcebgtrgpagf 0v_lrteb_2 7283cebcpatsxb_1 pf jt__ pf ql gwt uettmt3gwpj cebrtshet xgft_u4 hwxf jpf st`bafgepgts ql abltif^l tg p_47:B hfxav p u_hbetfrtart dhtarwxav gtrwaxdht jxgwgwt cebqt cletat4 ctitegwt_tff2 gwt relbcebgtrgpagf rbh_s cetitag fb`t rwpavtf xa gwt fgehrghet bu gwt `t`qepat cebgtxaf sht gb _bj gt`ctepghetf4 84 df`b`tgexr Mtwpixbe L et3tkp`xapgxba bu gwt bf`b`tgexr qtwpixbe bu wh`pa telgwebrlgtf ql kxtfg pas ggtcba^hf.,K 3 gp^xav xagb prrbhag pa bf`bgxrp__l xaprgxit jpgte ueprgxba 3 fwbjts pa p_`bfg xstp_ etfcbaft gb fbsxh` rw_bexst fb_hgxbaf xarbartagepgxbaf pf wxvwpf 94Abf`b__^v2 jwtetpf `pe^ts wt`b_lfxf p_etpsl qtvpa pg _bjte rbartagepgxbaf4 Lrrbesxav gb gwt atpe_l _xatpe Mbl_t3jpa.g Tbuu c_bg bqgpxats2 pqbhg 8:- bu gwt jpgte xa xfbgbaxr ets q_bbs rt__f xf bf`bgxrp__l xaprgxit4 hwt `pybe cpeg bu gwxfbf`bgxrp__l xaprgxit jpgte 0atpe_l CA-1 rpa qt prrbhagts ube ql gwt wlsepgxba bu wt`bv_bqxa4 Lssxgxbap_ uhasp`tagp_ tixstart rba3 rt`xav ctatgepgxav relbcebgtrgpagf jpf vxita ql btel`pa pas Pbhv_pf2', ap`t_l gwt pccebcexpgt rbartagepgxba bu fp_g be bgwte abactatgepgxav fb_hgt gwpg fwbh_s qt cetftag xa gwt tkgeprt__h_pe fcprt gb cetitag wt`b_lfxf pugte tdhx_xqepgxba bu gwt relbcebgtrgpag prebff gwt rt__ `t`qepat4 Uabeste gb pibxs p rwpavt xaib_h`t sht gb gwt pssxgxba bugwt relbcebgtrgpag2 gwt fp_g rbartagepgxba `hfg qt ^tcg rbafgpag pg p `b_pe 0`b__U fb_hgxba2 2$ epgwte gwpa p `b_p_ 0`b__^v T80, ;$ qpfxf4 Qfctrxp__l jwta _pevte ib_h`tf bu gwt relbcebgtrgxit rb`3

110

Clinical Applications of Cryobiology

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445

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