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Letter to Editor
Comments on: Assessment of acid phosphatase and alkaline phosphatase in gingival crevicular fluid in growing and adult orthodontic patients: An in vivo study
Having read the article titled, “Assessment of acid phosphatase and alkaline phosphatase in gingival crevicular fluid in growing and adult orthodontic patients: An in vivo study” (Smitha V. Shetty, Anand K. Patil, Sanjay V. Ganeshkar. J Ind Orthod Soc 2015; 49(1): 10‑14) with great interest, we really appreciate the combined efforts of the team for the study undertaken. This article has assessed both the enzymes acid phosphatase and alkaline phosphatase, which is commendable. It reveals the effects of these enzymes on growing patients and fills the research lacunae in that area.
increases with increase in force levels. The severity of crowding plays an important factor in the force levels generated by the archwires. Was there any standardization of the initial amount of crowding in both the groups to avoid any bias?
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However, we would like to know the following: This study was done on 14 patients in each of the two groups. What was the rationale behind the sample size selection and how the sample size determined? Moreover, the mean age group for the growing patients was erroneously mentioned as 11 ± 4 years because all the patients underwent upper 1st premolar extractions, and they would not have erupted in a 7‑year‑old patient. Was there equal gender distribution in both the groups? This is because gender has been proven to be a factor in enzyme secretion and also plays a role in the timing of growth spurt.[1] Two microliter of gingival crevicular fluid was collected from the maxillary canine area and diluted in phosphate buffer saline solution. However, the amount of dilution is not mentioned. This may have an effect on the biochemical assay. If the amount of dilution was mentioned, it would have been easier to compare the results of this study with similar studies. Since oral hygiene plays a significant role in the secretion of enzymes, how were they standardized in both the groups?[2] The time intervals in the text and table do not match. Was it at 1 week, 3 weeks, and 6 weeks? What was the level of significance for the paired and unpaired t‑test for the statistics? “A reversal phase of orthodontic tooth movement” is mentioned in the discussion. It would be helpful for postgraduate students if the necessary literature was quoted for further reading. In this study, the baseline level T0 was taken after the initial alignment and leveling. The level of enzymes generally
S Dilip1, K Ravi2 Professor, 2Professor and HOD Department of Orthodontics, SRM Dental College, Chennai, Tamil Nadu, India 1
1,2
Address for correspondence: Dr. S Dilip, AC 130, Shanthi Colony, Anna Nagar, Chennai - 600 040, Tamil Nadu, India. E-mail:
[email protected]
References 1. Proffit WR. Contemporary Orthodontics. 5th ed., Ch. 4. St. Louis: Mosby, An imprint of Elsevier; 2007. 2. Chapple IL, Glenwright HD, Matthews JB, Thorpe GH, Lumley PJ. Site‑specific alkaline phosphatase levels in gingival crevicular fluid in health and gingivitis: Cross‑sectional studies. J Clin Periodontol 1994;21:409‑14.
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www.jios.in DOI: 10.4103/0301-5742.171312
How to cite this article: Dilip S, Ravi K. Comments on: Assessment of acid phosphatase and alkaline phosphatase in gingival crevicular fluid in growing and adult orthodontic patients: An in vivo study. J Indian Orthod Soc 2015;49:233.
© 2015 Journal of Indian Orthodontic Society | Published by Wolters Kluwer - Medknow 233
